Rapid Detection of Three Foodborne Pathogenic Bacteria in School Catering by Isothermal Multiple Self-matching-initiated Amplification Technique

JIAO Qiang; CHEN Wansheng; ZHOU Nan; ZHANG Qingfa; LIU Zhiyong

doi:10.13386/j.issn1002-0306.2022110196

Volume 44 Issue 18

Sep. 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(18): 371-377. > DOI: 10.13386/j.issn1002-0306.2022110196

JIAO Qiang, CHEN Wansheng, ZHOU Nan, et al. Rapid Detection of Three Foodborne Pathogenic Bacteria in School Catering by Isothermal Multiple Self-matching-initiated Amplification Technique[J]. Science and Technology of Food Industry, 2023, 44(18): 371−377. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022110196.

Citation:

PDF (3528 KB)

Rapid Detection of Three Foodborne Pathogenic Bacteria in School Catering by Isothermal Multiple Self-matching-initiated Amplification Technique

1.
Henan Province Food Inspection Research Institute, Key Laboratory of Food Safety Quick Testing and Smart Supervision Technology for State Regulation, Zhengzhou 450000, China
2.
Guangzhou DI’AO Bio-technology Co., Ltd., Guangzhou 510507, China

More Information

Received Date: November 16, 2022
Available Online: July 13, 2023

Graphical Abstract

Abstract

Abstract

Objective: In this paper, a detection method suitable for rapid identification of three pathogenic bacteria from food in the school catering was studied. Method: Based on isothermal multiple self-matching-initiated amplification technology (IMSA), strain-specific primers were designed for Salmonella invA gene, Escherichia coli O157:H7/NM rfbE gene and Listeria monocytogenes prfA gene to test the sensitivity, specificity, shortest enrichment time, and minimum detection limit of bacterial content. Food microbiology inspection according to National Food Safety Standards (GB/T 4789.4-2016, GB/T 4789.30-2016, GB/T 4789.36-2016) were the reference methods, and compared the consistency of the two methods. Result: The results showed that the sensitivity of our method to Salmonella, Escherichia coli O157:H7/NM and Listeria monocytogenes were 2.14×10³, 2.79×10³ and 3.62×10³ CFU/mL respectively, the specificity was 100%, the shortest enrichment time of artificial contamination were 6, 8 and 8 hours respectively. The detection limits of the lowest bacteria content were 2.14, 2.79 and 3.62 CFU/25 g respectively, and the consistency of the results of 74 food samples using IMSA method and the reference method was 100%. Conclusion: IMSA technology of pathogenic bacteria detection in food had the advantage of having high sensitivity, strong specificity and accurate results, which could complete the detection of three pathogenic bacteria in food in a short time and was suitable for the rapid identification of pathogenic bacteria in school catering.

FullText(HTML)

References (26)

References

[1]	ROBERT L S, JOHN B, DONALD J S, et al. An economic evaluation of PulseNet: A network for foodborne disease surveillance[J]. American Journal of Preventive Medicine,2016,50(5S1):S66−S73.
[2]	岳思远, 苏亮, 任鹏程, 等. 食源性致病菌生长延滞期建模的研究进展[J]. 食品科学,2019,40(1):313−318. [YUE Saiyuan, SU Liang, REN Pengcheng, et al. Progress in modeling of foodborne pathogen growth at lag phase[J]. Food Science,2019,40(1):313−318. YUE Saiyuan, SU Liang, REN Pengcheng, et al. Progress in modeling of foodborne pathogen growth at lag phase[J]. Food Science, 2019, 40(1): 313-318.
[3]	樊晓洁. 食源性致病菌大肠杆菌O157: H7检测方法的研究进展[J]. 食品安全质量检测学报,2020,11(7):2144−2149. [FAN Xiaojie. Research progress on detection methods of foodborne pathogenic Escherichia coli O157: H7[J]. Journal of Food Safety and Quality,2020,11(7):2144−2149. FAN Xiaojie. Research progress on detection methods of foodborne pathogenic Escherichia coli O157: H7[J]. Journal of Food Safety and Quality, 2020, 11(7): 2144-2149.
[4]	SCALLAN E , HOEKSTRA R M , MAHON B E , et al. An assessment of the human health impact of seven leading foodborne pathogens in the United States using disability adjusted life years[J]. Epidemiology and Infection,2015,143(13):2795−2804. doi: 10.1017/S0950268814003185
[5]	刘卫德, 刘绪平, 章瑛, 等. 中成药中沙门氏菌实时荧光PCR检测法的建立及优化[J]. 中国医药生物技术,2022,17(1):59−63. [LIU Weide, LIU Xuping, ZHANG Ying, et al. Establishment and optimization of real-time fluorescent PCR detection method for Salmonella in Chinese patent medicine[J]. Chinese Medicinal Biotechnology,2022,17(1):59−63. LIU Weide, LIU Xuping, ZHANG Ying, et al. Establishment and optimization of real-time fluorescent PCR detection method for Salmonella in Chinese patent medicine[J]. Chinese Medicinal Biotechnology, 2022, 17(1): 59-63.
[6]	李琴, 仵军红, 贾涛. 学校及托幼机构食堂餐饮食品的风险评估[J]. 食品工业,2022,43(4):335−337. [LI Qin, WU Junhong, JIA Tao. Risk assessment of schools and kindergartens[J]. The Food Industry,2022,43(4):335−337. LI Qin, WU Junhong, JIA Tao. Risk assessment of schools and kindergartens[J]. The Food Industry, 2022, 43(4): 335-337.
[7]	郑玲玲, 陈仲元. 高校食堂快检实验室检测项目探讨[J]. 食品安全导刊,2022(3):1−3. [ZHENG Lingling, CHEN Zhongyuan. Discussion on the test items of fast inspection laboratory in university canteen[J]. China Food Safety Magazine,2022(3):1−3. ZHENG Lingling, CHEN Zhongyuan. Discussion on the test items of fast inspection laboratory in university canteen[J]. China Food Safety Magazine, 2022(3): 1-3.
[8]	DING XIONG, KAI NIE, LEI SHI, et al. Improved detection limit in rapid detection of human enterovirus 71 and coxsackievirus A16 by a novel reverse transcription-isothermal multiple-self-matching-initiated amplification assay[J]. Journal of Clinical Microbiology,2014,52(6):1862−70. doi: 10.1128/JCM.03298-13
[9]	李鑫娜, 聂凯, 马学军, 等. 埃博拉病毒扎伊尔亚型多引物自配引发等温扩增方法的建立[J]. 病毒学报,2016,32(1):1−6. [LI Xinna, NIE Kai, MA Xuejun, et al. Detection of the Zaire subtype of the Ebola virus by isothermal multiple self-matching initiated amplification[J]. Chinese Journal of Virology,2016,32(1):1−6. LI Xinna, NIE Kai, MA Xuejun, et al. Detection of the Zaire subtype of the Ebola virus by isothermal multiple self-matching initiated amplification[J]. Chinese Journal of Virology, 2016, 32(1): 1-7.
[10]	乐振窍, 许泽仰, 张细玲, 等. FQ-PCR与IMSA检测转基因豆奶外源基因的比较研究[J]. 大豆科学,2018,37(6):943−949,954. [LE Zhenqiao, XU Zeyang, ZHANG Xiling, et al. Comparison of FQ-PCR and IMSA for detecting exogenous genes in genetically modified soymilk[J]. Soybean Science,2018,37(6):943−949,954. LE Zhenqiao, XU Zeyang, ZHANG Xiling, et al. Comparison of FQ-PCR and IMSA for Detecting Exogenous Genes in Genetically Modified Soymilk[J]. Soybean Science, 2018, 37(6): 943-949, 954.
[11]	LIU Wenxin, YUAN Chaowen, ZHANG Liguo, et al. Establishment and application of isothermal multiple-self-matching-initiated amplification (IMSA) in detecting type II heat-labile enterotoxin of Escherichia coli[J]. Plos One,2019,14(5):1−14.
[12]	王琪, 徐文娟, 石盼盼. IMSA技术快速检测肠出血大肠杆菌O157: H7方法的建立及应用[J]. 食品工业科技,2021,42(17):263−269. [WANG Qi, XU Wenjuan, SHI Panpan. Development and application of IMSA for rapid detection of enterohaemorrhage Escherichia coli O157: H7[J]. Science and Technology of Food Industry,2021,42(17):263−269. WANG Qi, XU Wenjuan, SHI Panpan. Development and application of IMSA for rapid detection of enterohaemorrhage Escherichia coli O157: H7[J]. Science and Technology of Food Industry, 2021, 42(17): 263-269.
[13]	RAO S, LINKE L, DOSTER E, et al. Genomic diversity of class I integrons from antimicrobial resistant strains of Salmonella typhimurium isolated from livestock, poultry and humans[J]. PLoS One,2020,15(12):e0243477−e0243477. doi: 10.1371/journal.pone.0243477
[14]	KUPPUSWAMY N K, TOMAS D. Real-time PCR method for detection of Salmonella spp. in environmental samples[J]. Appl Environ Microbiol,2017,83(14):e00644−17.
[15]	ABENI B, ISOKEN H I, ETINOSA O I. Prevalence of antimicrobial resistance and virulence gene elements of Salmonella serovars from ready-to-eat (RTE) shrimps[J]. Frontiers in Microbiology,2019,10:1613. doi: 10.3389/fmicb.2019.01613
[16]	TÓTH ISTVÁN, BAGYINSZKY EVA, SVÁB DOMONKOS. Multiplex PCR typing scheme based on Escherichia coli O157: H7 Sakai prophage (Sp) associated genes[J]. International Journal of Infectious Diseases: IJID: Official Publication of the International Society for Infectious Diseases,2022,120:68−76. doi: 10.1016/j.ijid.2022.04.015
[17]	MOSTAFA B, FIROUZ E, SHAHRAM N, et al. Identification of E. coli O157: H7 by using specific primers forrfbE and stx2b genes[J]. Iranian South Medical Journal,2017,20(3):267−277.
[18]	WITTE A K, MESTER P, ROSSMANITH P. qPCR Validation on the basis of the Listeria monocytogenes prfA assay[J]. Methods Mol Biol,2021,2220:41−53.
[19]	AWANG M S, YAZMIN B, HAIRUL H H, et al. Advancement in Salmonella detection methods: From conventional to electrochemical-based sensing detection[J]. Biosensors,2021,11(9):346−346. doi: 10.3390/bios11090346
[20]	顾雨熹, 王锦, 陈帅, 等. 食品中两种沙门氏菌检测方法的比较[J]. 粮食储藏,2022,51(2):35−40. [GU Yuxi, WANG Jin, CHEN Shuai, et al. Comparison of two detection methods for Salmonella spp. in food[J]. Grain Storage,2022,51(2):35−40. GU Yuxi, WANG Jin, CHEN Shuai, et al. comparison of two detection methods for Salmonella spp. in food[J]. Grain Storage, 2022, 51(2): 35-40.
[21]	康招娣, 李红娜, 袁飞. 快速检测技术在食源性沙门氏菌检测中的应用研究进展[J]. 中国食品卫生杂志,2022,34(4):848−855. [KANG Zhaodi, LI Hongna, YUAN Fei. Research progress on the application of rapid detection technology for foodborne Salmonella[J]. Chinese Journal of Food Hygiene,2022,34(4):848−855. KANG Zhaodi, LI Hongna, YUAN Fei. Research progress on the application of rapid detection technology for foodborne Salmonella[J]. Chinese Journal of Food Hygiene, 2022, 34(4): 848-855.
[22]	WANG Minglu, ZHANG Yilun, TIAN Fangyuan, et al. Overview of rapid detection methods for Salmonella in foods: Progress and challenges[J]. Foods,2021,10(10):2402. doi: 10.3390/foods10102402
[23]	范龙兴, 宁保安, 孙智勇, 等. 基于化学发光磁酶免疫分析技术检测单增李斯特菌[J]. 食品研究与开发,2017,38(3):124−129. [FAN Longxing, NING Baoan, SUN Zhiyong, et al. Detection of Listeria monocytogenes through chemiluminescent magnetic enzyme immunoassay[J]. Food Research and Development,2017,38(3):124−129. FAN Longxing, NING Baoan, SUN Zhiyong, et al. Detection of Listeria monocytogenes through chemiluminescent magnetic enzyme immunoassay[J]. Food Research and Development, 2017, 38(3): 124-129.
[24]	苏丹, 吴天琪, 杨雨, 等. 量子点标记免疫分析技术在食品安全检测中的应用现状[J]. 食品研究与开发,2022,43(10):210−216. [SU Dan, WU Tianqi, YANG Yu, et al. Application of quantum dots-based immunoassay in food safety detection[J]. Food Research and Development,2022,43(10):210−216. SU Dan, WU Tianqi, YANG Yu, et al. Application of quantum dots-based immunoassay in food safety detection[J]. Food Research and Development, 2022, 43(10): 210-216.
[25]	NUGRAHA R, NURILMALA M, NURJANAH, et al. Detection of Salmonella sp. in fisheries product using real-time PCR[J]. IOP Conference Series:Earth and Environmental Science,2019,404:012012−012012.
[26]	CHEN Xuelong, WANG Honghai, LIU Chang, et al. Technical note: Development of a closed-tube isothermal multiple self-matching-initiated amplification assay for visual detection of Staphylococcus aureus in milk samples[J]. Journal of Dairy Science,2020,104(3):3569−3574.

[1]	YUE Huimin, LI Haixin, LUO Ruiping, ZHAO Liang. Study on the Enrichment Methods and Application of LAMP Technology for Detection of Listeria monocytogenes in Frozen Meat Products[J]. Science and Technology of Food Industry, 2021, 42(24): 128-135. DOI: 10.13386/j.issn1002-0306.2021050043
[2]	WANG Qi, XU Wenjuan, SHI Panpan. Development and Application of IMSA for Rapid Detection of Enterohaemorrhage Escherichia coli O157:H7[J]. Science and Technology of Food Industry, 2021, 42(17): 263-269. DOI: 10.13386/j.issn1002-0306.2020100219
[3]	YOU Zhen-dan, CHEN Chuan-jun, JIANG Yu-han, PU Chun-ru, JIANG Yan, DU Juan-lan, ZHANG Bai-yu, YAN Li-heng, YAN Qi-gui, HAN Guo-quan. Research Progress of Rapid Detection of Listeria monocytogenes in Ready-to-eat Food[J]. Science and Technology of Food Industry, 2020, 41(10): 358-362. DOI: 10.13386/j.issn1002-0306.2020.10.060
[4]	ZHONG Hai-xia, ZHOU Han-yan, LUO Huan, XU Yi, ZHANG Ren-yu, YANG Zheng-ming, CHEN Zhi-guang. Research Progress of Isothermal Amplification in the Detection of Pathogenic Bacteria in Food[J]. Science and Technology of Food Industry, 2019, 40(7): 362-367. DOI: 10.13386/j.issn1002-0306.2019.07.063
[5]	MA Hui-juan, XU Hui, LI Li-li, LIU Shu, JIA Tao, LI Xin-li, GAO Hong, ZHANG Hou-sen. Comparison of two methods for detection of Listeria monocytogenes in foods[J]. Science and Technology of Food Industry, 2018, 39(7): 263-267. DOI: 10.13386/j.issn1002-0306.2018.07.047
[6]	WANG Jian-chang, HU Lian-xia, SUN Xiao-xia, LI Jing. Establishment of real time fluorescence single primer isothermal amplification for Listeria monocytogenes[J]. Science and Technology of Food Industry, 2017, (16): 275-279. DOI: 10.13386/j.issn1002-0306.2017.16.052
[7]	CUI Hai-ying, BAI Mei, DAI Jin-ming, TAO Gui-ze, LIN Lin. Antimicrobial activity of cold nitrogen plasma against Escherichia coli O157∶ H7 biofilms on cucumber[J]. Science and Technology of Food Industry, 2017, (02): 162-165. DOI: 10.13386/j.issn1002-0306.2017.02.022
[8]	FENG Ke, HU Wen-zhong, JIANG Ai-li, Xu Yong-ping, SA-ren-gao-wa. Research progress in molecular biology detection methods of Listeria monocytogenes[J]. Science and Technology of Food Industry, 2014, (04): 392-396. DOI: 10.13386/j.issn1002-0306.2014.04.044
[9]	WANG Fei, ZHANG Ji-lun, NING Xi-bin. Antibacterial effect of compound lactobacillus on E.coli O157∶H7[J]. Science and Technology of Food Industry, 2013, (21): 83-86. DOI: 10.13386/j.issn1002-0306.2013.21.047
[10]	Resarch progress in rapid detection of Listeria monocytogenes in fresh agricultural products[J]. Science and Technology of Food Industry, 2013, (11): 361-364. DOI: 10.13386/j.issn1002-0306.2013.11.062

Supplements (0)

Cited By

Cited by

Periodical cited type(3)

1.	邹婕，杨漫漫，胡晓亮，田志革. 沙门氏菌快速检测技术研究进展. 质量安全与检验检测. 2024(02): 73-78 .
2.	牛会敏，焦强，李若菡，刘智勇. 学校食堂餐饮食品加工过程致病性微生物监测结果. 中国学校卫生. 2024(06): 895-898 .
3.	孙千雪，周炳武，王婷，蔡琳雅，胡珊珊，刘晔. 基于分子生物学方法的食品中肠出血性大肠杆菌O157∶H7检测技术研究进展. 粮食与油脂. 2024(11): 6-13 .