Effect of Ammonia Pretreatment on Enzymatic Sugar Production from Corn Stover

LI Jianxun; LI Xin; WANG Yumeng; ZHANG Wenjuan; WEI Jiping; SONG Wenjun

doi:10.13386/j.issn1002-0306.2022090155

Volume 44 Issue 12

Jun. 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(12): 216-222. > DOI: 10.13386/j.issn1002-0306.2022090155

LI Jianxun, LI Xin, WANG Yumeng, et al. Effect of Ammonia Pretreatment on Enzymatic Sugar Production from Corn Stover[J]. Science and Technology of Food Industry, 2023, 44(12): 216−222. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090155.

Citation:

PDF (3522 KB)

Effect of Ammonia Pretreatment on Enzymatic Sugar Production from Corn Stover

1.
College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China
2.
College of Biotechnology, Tianjin Modern Vocational and Technical College, Tianjin 300350, China

More Information

Received Date: September 14, 2022
Available Online: April 19, 2023

Graphical Abstract

Abstract

Abstract

To make comprehensive use of maize stover, cellulase degradation of maize stover was accelerated. In this paper, maize straw was used as raw material, and reducing sugar yield was the main indicator. The reducing sugar yield was determined by the DNS method after enzymatic digestion of maize straw by ammonification pretreatment, and the effects of ammonification agent type, concentration, solid content and ammonification time on the enzymatic sugar production from maize straw were investigated. The results showed that the highest reducing sugar yield of 314.18 mg/mL was achieved under this condition with ammonium carbonate as the ammoniating agent, ammoniating agent concentration of 20%, solid content of 50% and ammoniating time of 11 d, which was 51.80% higher compared to direct enzymatic digestion of the straw. In addition, it was found by SEM that the structure of lignin and cellulose changed after the ammoniated pretreatment of rice straw with ammonium carbonate, with the surface structure becoming rough and loose and the cellulose exposed, which was more favorable to the action of cellulase. In addition, FTIR revealed that the absorption peaks of maize straw at 2920 and 1650 cm⁻¹ were weakened after ammoniation treatment, and the reduction of their peaks represented to some extent the destruction of the lignin structure. Overall, the enzymatic digestion of maize stover was more favorable after the ammoniated pretreatment of maize stover with ammonium carbonate.
- maize stover,
- lignin,
- cellulose,
- ammoniated pretreatment,
- cellulase

FullText(HTML)

References (35)

References

[1]	丁攀, 叶芳, 张轲, 等. 玉米秸秆资源的综合利用及方式[J]. 河南农业大学学报,2020(16):17. [DING P, YE F, ZHANG K, et al. Comprehensive utilization and methods of corn stover resources[J]. Agriculture of Henan,2020(16):17. DING P, YE F, ZHANG K, et al. Comprehensive utilization and methods of corn stover resources[J]. Agriculture of Henan, 2020(16): 17.
[2]	杜林雪. 玉米秸秆生态循环利用研究[D]. 邯郸: 河北工程大学, 2022 DU L X. Study on ecological recycling of corn straw[D]. Handan: Hebei University of Engineering, 2022.
[3]	王立新. 玉米秸秆综合利用现状和发展前景[J]. 农业开发与装备,2021(1):66−67. [WANG L X. Present situation and development prospect of comprehensive utilization of corn stover[J]. Agricultural Development & Equipments,2021(1):66−67. doi: 10.3969/j.issn.1673-9205.2021.01.030 WANG L X. Present situation and development prospect of comprehensive utilization of corn stover[J]. Agricultural Development & Equipments, 2021(1): 66-67. doi: 10.3969/j.issn.1673-9205.2021.01.030
[4]	郝展宏, 沙野, 米国华. 东北地区玉米秸秆覆盖技术应用现状与对策[J]. 玉米科学,2021,29(3):100−110. [HAO Z H, SHA Y, MI G H. Current application status of maize residue mulching in Northeast China and the policy recommendation[J]. Journal of Maize Sciences,2021,29(3):100−110. HAO Z H, SHA Y, MI G H. Current application status of maize residue mulching in Northeast China and the policy recommendation[J]. Journal of Maize Sciences, 2021, 29(3): 100-110.
[5]	孙炬仁. 玉米秸秆营养成分的影响因素及不同收获期对饲用价值的影响研究进展[J]. 饲料研究,2021,44(18):154−157. [SUN J R. Research progress on influencing factors of corn stalk nutritional components and the effect of different harvest periods on feeding value[J]. Feed Research,2021,44(18):154−157. [SUN J R. Research progress on influencing factors of corn stalk nutritional components and the effect of different harvest periods on feeding value[J]. Feed Research, , 2021, 44(18): 154-157.
[6]	GAO D H, HAARMEYER C, BALAN V, et al. Lignin triggers irreversible cellulase loss during pretreated lignocellulosic biomass saccharification[J]. Biotechnology for Biofuels,2014,7:175. doi: 10.1186/s13068-014-0175-x
[7]	徐鹏, 徐晓东, 李洋, 等. 木质纤维素降解菌在秸秆堆肥中的研究现状与进展[J]. 北方园艺,2022(8):122−128. [XU P, XU X D, LI Y, et al. Research status and progress of lignocellulosic degrading bacteria in straw compost[J]. Northern Horticulture,2022(8):122−128. XU P, XU X D, LI Y, et al. Research status and progress of lignocellulosic degrading bacteria in straw compost[J]. Northern Horticulture, 2022(8): 122-128.
[8]	ZHANG L, YOU T, ZHOU T, et al. Synergistic effect of white-rot fungi and alkaline pretreatments for improving enzymatic hydrolysis of poplar wood[J]. Industrial Crops and Products,2016,86:155−162. doi: 10.1016/j.indcrop.2016.03.041
[9]	HANS M, GARG S, PELLEGRINI V O A, et al. Liquid ammonia pretreatment optimization for improved release of fermentable sugars from sugarcane bagasse[J]. Journal of Cleaner Production,2021,281:123922. doi: 10.1016/j.jclepro.2020.123922
[10]	崔美, 黄仁亮, 苏荣欣, 等. 木质纤维素新型预处理与顽抗特性[J]. 化工学报,2012,63(3):677−687. [CUI M, HUANG R L, SU R X, et al. An overview on lignocellulose pretreatment and recalcitrant characteristics[J]. CIESC Journal,2012,63(3):677−687. doi: 10.3969/j.issn.0438-1157.2012.03.002 CUI M, HUANG R L, SU R X, et al. An overview on lignocellulose pretreatment and recalcitrant characteristics[J]. CIESC Journal, 2012, 63(03): 677-687. doi: 10.3969/j.issn.0438-1157.2012.03.002
[11]	LI X, SHI Y, KONG W, et al. Improving enzymatic hydrolysis of lignocellulosic biomass by bio-coordinated physicochemical pretreatment-A review[J]. Energy Reports,2022,8:696−709.
[12]	DONG L L, CAO G L, WU J W, et al. High-solid pretreatment of rice straw at cold temperature using NaOH/Urea for enhanced enzymatic conversion and hydrogen production[J]. Bioresource Technology, 2019, 287: 121399.
[13]	何士成, 彭太兵, 孙曼钰, 等. 碱处理中温度对不同底物特性木质纤维素结构及酶解的影响[J]. 江苏农业科学,2017,45(21):292−296. [HE S C, PENG T B, SUN M Y, et al. Effect of temperature on structure and enzymatic hydrolysis of lignocellulose with different substrate properties in alkali treatment[J]. Jiangsu Agricultural Sciences,2017,45(21):292−296. HE S C, PENG T B, SUN M Y, et al. Effect of temperature on structure and enzymatic hydrolysis of lignocellulose with different substrate properties in alkali treatment[J]. Jiangsu Agricultural Sciences, 2017, 45(21): 292-296.
[14]	AGBOR V B, CICEK N, SPARLING R, et al. Biomass pretreatment: Fundamentals toward application[J]. Biotechnology Advances,2011,29(6):675−685. doi: 10.1016/j.biotechadv.2011.05.005
[15]	罗立娜, 丁清华, 公维佳, 等. 尿素氨化预处理改善稻秸干法厌氧发酵特性[J]. 农业工程学报,2015,31(19):234−239. [LUO L N, DING Q H, GONG W J, et al. Urea ammoniated pretreatment improving dry anaerobic fermentation characteristics of rice straw[J]. Transactions of the Chinese Society of Agricultural Engineering,2015,31(19):234−239. doi: 10.11975/j.issn.1002-6819.2015.19.033 LUO L N, DING Q H, GONG W J, et al. Urea ammoniated pretreatment improving dry anaerobic fermentation characteristics of rice straw[J]. Transactions of the Chinese Society of Agricultural Engineering, 2015, 31(19): 234-239. doi: 10.11975/j.issn.1002-6819.2015.19.033
[16]	王忠江, 邹舰洋, 曹振, 等. 尿素预处理玉米秸秆降解木质素动力学研究[J]. 农业机械学报,2020,51(11):321−328. [WANG Z J, ZOU J Y, CAO Z, et al. Delignification kinetics of corn stover with urea pretreatment[J]. Transactions of the Chinese Society for Agricultural Machinery,2020,51(11):321−328. doi: 10.6041/j.issn.1000-1298.2020.11.035 WANG Z J, ZOU J Y, CAO Z, et al. Delignification kinetics of corn stover with urea pretreatment[J]. Transactions of the Chinese Society for Agricultural Machinery, 2020, 51(11): 321-328. doi: 10.6041/j.issn.1000-1298.2020.11.035
[17]	ZHANG H, WU J. Statistical optimization of aqueous ammonia pretreatment and enzymatic saccharification of corn stover for enhancing sugars production[J]. Environmental Technology & Innovation,2022,26:102238.
[18]	HUANG X, DING Y, LIAO X, et al. Microbial lipid production from enzymatic hydrolysate of corn stover pretreated by combining with biological pretreatment and alkalic salt soaking[J]. Industrial Crops and Products,2018,124:487−494. doi: 10.1016/j.indcrop.2018.08.030
[19]	YUAN H R, ZHANG Y T, LI X J, et al. Effects of ammoniation pretreatment at low moisture content on anaerobic digestion performance of rice straw[J]. Bioresources,2014,9(4):6707−6718.
[20]	LI X A, KIM T H. Low-liquid pretreatment of corn stover with aqueous ammonia[J]. Bioresource Technology,2011,102(7):4779−4786. doi: 10.1016/j.biortech.2011.01.008
[21]	宋振华, 朱潇静, 姜文廷, 等. 不同氨处理对玉米秸秆糖化效果的影响[J]. 江西农业学报,2019,31(6):107−110. [SONG Z H, ZHU X J, JIANG W Y, et al. Effect of different ammonia pretreatments on saccharification of corn straw[J]. Acta Agriculturae Jiangxi,2019,31(6):107−110. SONG Z H, ZHU X J, JIANG W Y, et al. Effect of different ammonia pretreatments on saccharification of corn straw[J]. Acta Agriculturae Jiangxi, 2019, 31(6): 107-110.
[22]	裴芳霞. 超声波辅助黄孢原毛平革菌对酒糟酶解糖化的影响[D]. 兰州: 兰州理工大学, 2016 PEI F X. Effect ofultrasound-assisted Phanerochaete chrysosporium pretreatment on enzymatic saccharification of distillers grains[D]. Lanzhou: Lanzhou University of Technology, 2016.
[23]	张明珠, 郝明欣, 桑喆. 玉米淀粉的扫描电镜分析方法[J]. 理化检验-物理分册,2022,58(8):21−24,28. [ZHANG M Z, HAO M X, SANG J. Scanning electron microscope analysis method for corn starch J]. Physical Testing and Chemical Analysis (Part A:Physical Testing),2022,58(8):21−24,28.
[24]	张立娟, 王书涛, 杨哲, 等. XRF, PXRD和FTIR的广藿香主要组分测定与表征[J]. 光谱学与光谱分析,2017,37(12):3889−3892. [ZHANG L J, WANG S T, YANG Z, et al. The determination and xharacterization of main components in patchouli based on the XRF, pXRD and FTIR[J]. Spectroscopy and Spectral Analysis,2017,37(12):3889−3892. ZHANG L J, WANG S T, YANG Z, et al. The determination and xharacterization of main components in patchouli based on the XRF, pXRD and FTIR[J]. Spectroscopy and Spectral Analysis, 2017, 37(12): 3889-3892.
[25]	徐忠, 汪群慧, 姜兆华. 氨预处理对大豆秸秆纤维素酶解产糖影响的研究[J]. 高校化学工程学报,2004(6):773−776. [XU Z, WANG Q H, JIANG Z H. Effect of ammonia pretreatment on sugar production by cellulose hydrolysis of soybean straw[J]. Journal of Chemical Engineering of Chinese Universities,2004(6):773−776. doi: 10.3321/j.issn:1003-9015.2004.06.019 XU Z, WANG Q H, JIANG Z H. Effect of ammonia pretreatment on sugar production by cellulose hydrolysis of soybean straw[J]. Journal of Chemical Engineering of Chinese Universities, 2004, (06): 773-776. doi: 10.3321/j.issn:1003-9015.2004.06.019
[26]	WANG L L, ZHANG K, XU Y, et al. High-solid pretreatment of corn stover using urea for enzymatic saccharification[J]. Bioresource Technology,2018,259:83−90. doi: 10.1016/j.biortech.2018.03.023
[27]	AHMED M A, KIM I, KIM G Y, et al. Ammonium carbonate as a catalyst for lignocellulose pretreatment and a nitrogen source for fermentation[J]. Sustainable Energy Technologies and Assessments,2016,16:64−68. doi: 10.1016/j.seta.2016.05.002
[28]	马旭光, 刘素君, 江滔, 等. 高含固率木质纤维素厌氧发酵物总DNA的4种提取方法比较[J]. 中国沼气,2020,38(2):28−35. [MA X G, LIU S J, JIANG T, et al. Comparison of four methods for extracting microbial total DNA from lignocellulosic digestate with high solid content[J]. China Biogas,2020,38(2):28−35. doi: 10.3969/j.issn.1000-1166.2020.02.004 [MA X G, LIU S J, JIANG T, et al. Comparison of four methods for extracting microbial total DNA from lignocellulosic digestate with high solid content[J]. China Biogas, 2020, 38(2): 28-35. doi: 10.3969/j.issn.1000-1166.2020.02.004
[29]	吴晋锴, 孙辰, 刘荣厚. 氨处理时间对小麦秸秆厌氧发酵产沼气的影响[J]. 太阳能学报,2013,34(9):1547−1550. [WU J K, SUN C, LIU R H. Effects of urea treatment time on anaerobic fermentation of wheat straw biogas production[J]. Acta Energiae Solaris Sinica,2013,34(9):1547−1550. doi: 10.3969/j.issn.0254-0096.2013.09.010 [WU J K, SUN C, LIU R H. Effects of urea treatment time on anaerobic fermentation of wheat straw biogas production[J]. Acta Energiae Solaris Sinica. 2013, 34(09): 1547-1550. doi: 10.3969/j.issn.0254-0096.2013.09.010
[30]	KIM I, LEE B, SONG D, et al. Effects of ammonium carbonate pretreatment on the enzymatic digestibility and structural features of rice straw[J]. Bioresource Technology,2014,166:353−357. doi: 10.1016/j.biortech.2014.04.101
[31]	KIM I, REHMAN M S U, HAN J I. Enhanced glucose yield and structural characterization of corn stover by sodium carbonate pretreatment[J]. Bioresource Technology,2014,152:316−320. doi: 10.1016/j.biortech.2013.10.069
[32]	YOO C G, KIM H, LU F C, et al. Understanding the physicochemical characteristics and the improved enzymatic saccharification of corn stover pretreated with aqueous and gaseous ammonia[J]. Bioenergy Research,2016,9(1):67−76. doi: 10.1007/s12155-015-9662-6
[33]	吴雪君. 糙皮侧耳降解利用木质纤维素的机理研究[D]. 北京: 北京林业大学, 2017. WU X J. A study on the mechanism of degradation and utilization of lignocellulose by Pleurotus ostreatus [D]. Beijing, Beijing Forestry University, 2017.
[34]	邢军梅. 微波结合P. chrysosporium预处理对酒糟酶解糖化效果的影响[D]. 兰州: 兰州理工大学, 2017 XING J M. Combined effect of microwave and P. chrysosporium pretreatments of distillers grains for fermentable sugars by enzymatic hydrolysis[D]. Lanzhou: Lanzhou University of Technology, 2017.
[35]	赵超. 玉米秸秆液氨/过氧化氢联合预处理的试验研究[D]. 南京: 南京农业大学, 2014 ZHAO C. Expermental study on the combined ammonia/hydrogen peroxide peroxide pretreatment of cornstover [D]. Nanjing: Nanjing Agricultural University, 2014.