Effect of Ammonia Pretreatment on Enzymatic Sugar Production from Corn Stover

LI Jianxun; LI Xin; WANG Yumeng; ZHANG Wenjuan; WEI Jiping; SONG Wenjun

doi:10.13386/j.issn1002-0306.2022090155

Volume 44 Issue 12

Jun. 2023

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Science and Technology of Food Industry > 2023 > 44(12): 216-222. > DOI: 10.13386/j.issn1002-0306.2022090155

LI Jianxun, LI Xin, WANG Yumeng, et al. Effect of Ammonia Pretreatment on Enzymatic Sugar Production from Corn Stover[J]. Science and Technology of Food Industry, 2023, 44(12): 216−222. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090155.

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Effect of Ammonia Pretreatment on Enzymatic Sugar Production from Corn Stover

1.
College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China
2.
College of Biotechnology, Tianjin Modern Vocational and Technical College, Tianjin 300350, China

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Received Date: September 14, 2022
Available Online: April 19, 2023

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Abstract

Abstract

To make comprehensive use of maize stover, cellulase degradation of maize stover was accelerated. In this paper, maize straw was used as raw material, and reducing sugar yield was the main indicator. The reducing sugar yield was determined by the DNS method after enzymatic digestion of maize straw by ammonification pretreatment, and the effects of ammonification agent type, concentration, solid content and ammonification time on the enzymatic sugar production from maize straw were investigated. The results showed that the highest reducing sugar yield of 314.18 mg/mL was achieved under this condition with ammonium carbonate as the ammoniating agent, ammoniating agent concentration of 20%, solid content of 50% and ammoniating time of 11 d, which was 51.80% higher compared to direct enzymatic digestion of the straw. In addition, it was found by SEM that the structure of lignin and cellulose changed after the ammoniated pretreatment of rice straw with ammonium carbonate, with the surface structure becoming rough and loose and the cellulose exposed, which was more favorable to the action of cellulase. In addition, FTIR revealed that the absorption peaks of maize straw at 2920 and 1650 cm⁻¹ were weakened after ammoniation treatment, and the reduction of their peaks represented to some extent the destruction of the lignin structure. Overall, the enzymatic digestion of maize stover was more favorable after the ammoniated pretreatment of maize stover with ammonium carbonate.
- maize stover,
- lignin,
- cellulose,
- ammoniated pretreatment,
- cellulase

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References (35)

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