Screening, Identification and Biological Characterization of <i>Weizmannia coagulans</i> BC66

KONG Sufen; ZHU Mingming; ZHU Jianguo; FANG Shuguang

doi:10.13386/j.issn1002-0306.2022080352

Volume 44 Issue 15

Jul. 2023

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Science and Technology of Food Industry > 2023 > 44(15): 134-142. > DOI: 10.13386/j.issn1002-0306.2022080352

KONG Sufen, ZHU Mingming, ZHU Jianguo, et al. Screening, Identification and Biological Characterization of Weizmannia coagulans BC66[J]. Science and Technology of Food Industry, 2023, 44(15): 134−142. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080352.

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Screening, Identification and Biological Characterization of Weizmannia coagulans BC66

Wuhan Wecare Probiotic Research Institute Co., Ltd., Wuhan 430000, China

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Received Date: September 01, 2022
Available Online: June 05, 2023

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Abstract

Abstract

The objective of this study was to isolate and screen probiotics for application in production of microecological preparation. A bacillus strain was screened from the feces of a healthy piglet based on heat treatment and lactic acid-producing characteristics. This strain was identified to be Weizmannia coagulans assigned as BC66 by means of morphological, physiological, biochemical and molecular identification. The strain Weizmannia coagulans BC66 was evaluated in vitro for its probiotic potential viz., the tolerance to gastrointestinal tract conditions (low pH, bile salt tolerance), antimicrobial activity, antibiotic susceptibility. The strain BC66 showed the resistance to low acidity and high bile salts concentration with the survival rate of 98% at pH3.0 and 79% at bile salt 0.3% respectively. The viability of the strain was 92% after 10 min treatment in 90 ℃ water bath. Moreover, the strain was able to act against Escherichia coli and Salmonella and was sensitive to most of the antibiotics tested. Strain BC66 did not exhibit any hemolytic activity. The results indicated that Weizmannia coagulans BC66 had excellent probiotic properties. This strain could be used as a potential candidate probiotic and eventually be explored further for potential application in feed additives.
- Weizmannia coagulans,
- screening,
- antimicrobial activity,
- sensitive,
- microecological preparation

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References

[1]	HAMMER B W. Bacteriological studies on the coagulation of evaporated milk[J]. Iowa Agriculture Experiment Station Research Bulletin,1915(19):119−132.
[2]	NYANGALE E, FARMER S, CASH H, et al. Bacillus coagulans GBI-30, 6086 modulates Faecalibacterium prausnitzii in older men and women[J]. The Journal of Nutrition,2015,145(7):1446−1452. doi: 10.3945/jn.114.199802
[3]	陈秀秀, 孙洪浩, 孙小涵, 等. 一株凝结芽孢杆菌的分离鉴定及益生性分析[J]. 饲料博览,2020(11):1−5. [CHEN X X, SUN H H, SUN X H, et al. Analysis of probiotic and isolation, identification of Bacillus coagulans[J]. Feed Review,2020(11):1−5. CHEN X X, SUN H H, SUN X H, et al. Analysis of probiotic and isolation, identification of Bacillus coagulans[J]. Feed Review, 2020(11): 1-5.
[4]	ZHOU Y H, ZENG Z H, XU Y B, et al. Application of Bacillus coagulans in animal husbandry and its underlying mechanisms[J]. Animals,2020,10(3):454. doi: 10.3390/ani10030454
[5]	JAMES G, DAS B, JOSE S, et al. Bacillus as an aquaculture friendly microbe[J]. Aquaculture International,2021,29:323−353. doi: 10.1007/s10499-020-00630-0
[6]	SAXENA A K, KUMAR M, CHAKDAR H, et al. Bacillus species in soil as a natural resource for plant health and nutrition[J]. Journal of Applied Microbiology,2019,128:1583−1594.
[7]	WANG J, ZHAO L. Study on the application of Bacillus coagulans to purify aquaculture water[J]. Advanced Materials Research,2012,343-344:988−993.
[8]	刘彬, 粟胜兰, 张雅惠, 等. 饲用凝结芽孢杆菌的菌种特性及作用机制[J]. 饲料工业,2022,43(5):40−44. [LIU B, LI S L, ZHANG Y H, et al. Strain property and action mechanism of Bacillus coagulans in feed[J]. Feed Industry,2022,43(5):40−44. LIU B, LI S L, ZHANG Y H, et al. Strain property and action mechanism of Bacillus coagulans in feed[J]. Feed Industry, 2022, 43(5): 40-44.
[9]	苗华彪, 李正洲, 赵元昊, 等. 凝结芽孢杆菌在生猪养殖中的应用及其作用机理的研究进展[J]. 中国畜牧兽医,2020,47(8):2433−2444. [MIAO H B, LI Z Z, ZHAO Y H, et al. Advance on the application and mechanism of Bacillus coagulans in pig breeding[J]. China Animal Husbandry and Veterinary Medicine,2020,47(8):2433−2444. MIAO H B, LI Z Z, ZHAO Y H, et al. Advance on the application and mechanism of Bacillus coagulans in pig breeding[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2433-2444.
[10]	辛国芹, 汪祥燕, 曹斌, 等. 一株产酸凝结芽孢杆菌的筛选及耐受特性研究[J]. 中国酿造,2022,41(4):111−115. [XIN G Q, WANG X Y, CAO B, et al. Screening and tolerance property of an acid-producing Bacillus coagulans[J]. China Brewing,2022,41(4):111−115. XIN G Q, WANG X Y, CAO B, et al. Screening and tolerance property of an acid-producing Bacillus coagulans[J]. China Brewing, 2022, 41(4): 111-115.
[11]	胡鹏钰, 于俊娟, 王鹏, 等. 母乳来源益生菌的筛选及潜在益生特性研究[J]. 食品与发酵工业,2021,47(14):190−195. [HU P Y, YU J J, WANG P, et al. Screening of probiotics from breast milk and its potential probiotic characteristics[J]. Food and Fermentation Industries,2021,47(14):190−195. HU P Y, YU J J, WANG P, et al. Screening of probiotics from breast milk and its potential probiotic characteristics[J]. Food and Fermentation Industries, 2021, 47(14): 190-195.
[12]	吴丹阳. 凝结芽孢杆菌T242益生性及功能特性的研究[D]. 大连: 大连工业大学, 2016. WU D Y. Study on probiotic properties and functionality of Bacillus coagulans T242[D]. Dalian: Dalian Polytechnic University, 2016.
[13]	方曙光, 孔素芬, 刘建明. 一种凝结芽孢杆菌菌粉芽孢的检测方法: 中国, 202010644874.6[P]. 2020-10-30. FANG S G, KONG S F, LIU J M. A method for detecting Bacillus coagulans powder spores: China, 202010644874.6[P]. 2020-10-30.
[14]	董文豪, 宫晓炜, 郑福英, 等. 猪源地衣芽孢杆菌的分离筛选及其特性[J]. 甘肃农业大学学报,2021(5):8−11. [DONG W H, GONG X W, ZHENG F Y, et al. Isolation, screening and biological characteristics of Bacillus licheniformis from pigs[J]. Journal of Gansu Agricultural University,2021(5):8−11. DONG W H, GONG X W, ZHENG F Y, et al. Isolation, screening and biological characteristics of Bacillus licheniformis from pigs[J]. Journal of Gansu Agricultural University, 2021(5): 8-11.
[15]	CHIDRE P, REVANASIDDAPPA K C, et al. Probiotic potential of Lactobacilli with antagonistic activity against pathogenic strains: An in vitro validation for the production of inhibitory substances[J]. Biomedical Journal,2017,40(5):270−283. doi: 10.1016/j.bj.2017.06.008
[16]	徐秀伟. 饲用抑菌活性乳酸菌共培养筛选、优化及抗性分析[D]. 合肥: 安徽农业大学, 2016. XU X W. Screening and optimization of fodder antibacterial lactic acid bacteria co-culture and resistance analysis[D]. Hefei: Anhui Agricultural University, 2016.
[17]	HALDER D, MANDA M, CHATTERJEE S S, et al. Indigenous probiotic lactobacillus isolates presenting antibiotic like activity against human pathogenic bacteria[J]. Biomedicines,2017,5(31):1−11.
[18]	余祖玲, 余祖华, 贾艳艳, 等. 降解纤维素凝结芽孢杆菌的分离鉴定及生物学特性研究[J]. 河南科技大学学报,2022,43(5):84−91, 99. [YU Z L, YU Z H, JIA Y Y, et al. Isolation, identificationand biological characteristic of acellulase-producing Bacillus coagulans[J]. Journal of Henan University of Science and Technology (Natural Science),2022,43(5):84−91, 99. YU Z L, YU Z H, JIA Y Y, et al. Isolation, identificationand biological characteristic of acellulase-producing Bacillus coagulans[J]. Journal of Henan University of Science and Technology (Natural Science), 2022, 43(5): 84-91, 99.
[19]	严涛, 朱建国, 姜甜, 等. 一株凝结芽孢杆菌的分离筛选及产孢条件优化[J]. 微生物学通报,2018,45(2):238−249. [YAN T, ZHU J G, JIANG T, et al. Isolation and optimization on spore-forming conditions of Bacillus coagulans[J]. Microbiology China,2018,45(2):238−249. YAN T, ZHU J G, JIANG T, et al. Isolation and optimization on spore-forming conditions of Bacillus coagulans[J]. Microbiology China, 2018, 45(2): 238-249.
[20]	布坎南. 伯杰细菌鉴定手册[M]. 中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组, 译. 北京: 科学出版社, 1984: 744−745. BUCHANAN. Berger bacterial identification manual[M]. Translation Group of "Berger Bacterial Identification Manual", Institute of Microbiology, Chinese Academy of Sciences, trans. Beijing: Science Press, 1984: 744−745.
[21]	余培斌, 杜晶, 陈建新, 等. 高温好氧堆肥过程中芽孢杆菌的筛选、鉴定及应用[J]. 食品与发酵工业,2020,46(12):199−205, 212. [YU P B, DU J, CHEN J X, et al. Study on screening and identificotion of Bacillus in the process of high-temperature aerobic composting and its application[J]. Food and Fermentation Industries,2020,46(12):199−205, 212. YU P B, DU J, CHEN J X, et al. Study on screening and identificotion of Bacillus in the process of high-temperature aerobic composting and its application[J]. Food and Fermentation Industries , 2020 , 46(12): 199– 205, 212.
[22]	柯轲, 方端, 高福, 等. 凝结芽孢杆菌生物学特性、益生机制及其生物学功能[J]. 畜禽业,2021(4):6−9. [KE K, FANG D, GAO F, et al. Biological characteristics, probiotic mechanism and biological functions of Bacillus coagulans[J]. Livestock and Poultry Industry,2021(4):6−9. KE K, FANG D, GAO F, et al. Biological characteristics, probiotic mechanism and biological functions of Bacillus coagulans[J]. Livestock and Poultry Industry, 2021(4): 6-9.
[23]	SNOEDK V, COX E, VERDONCK F, et al. Influence of porcine intestinal pH and gastric digestion on antigenicity of F4 fimbriae for oral immunization[J]. Veterinary Microbiology,2004,98(1):45−53. doi: 10.1016/j.vetmic.2003.10.020
[24]	HYRONIMUS B, LE M C, HADJ S A, et al. Acid and bile tolerance of spore-forming lactic acid bacteria[J]. International Journal of Food Microbiology,2000(61):193−197.
[25]	高盛, 乔宇, 张宇微, 等. 人母乳源乳酸菌的筛选、鉴定及益生活性的初步研究[J]. 食品工业科技,2017(10):205−210. [GAO S, QIAO Y, ZHANG Y W, et al. Isolation, identification and probiotic characterization of latic acid bacteria in human breast milk[J]. Science and Technology of Food Industry,2017(10):205−210. GAO S, QIAO Y, ZHANG Y W, et al. Isolation, identification and probiotic characterization of latic acid bacteria in human breast milk[J]. Science and Technology of Food Industry, 2017(10): 205-210.
[26]	涂家霖, 赵珊, 周钦育, 等. 凝结芽孢杆菌13002产芽孢条件优化[J]. 食品工业科技,2021,42(6):88−96, 102. [TU J L, ZHAO S, ZHOU Q Y, et al. Optimizing spore-forming conditions of Bacillus coagulans 13002[J]. Science and Technology of Food Industry,2021,42(6):88−96, 102. TU J L, ZHAO S, ZHOU Q Y, et al. Optimizing spore-forming conditions of Bacillus coagulans 13002[J]. Science and Technology of Food Industry, 2021, 42(6): 88-96, 102.
[27]	金迅, 刘冬梅, 阮晖, 等. 凝结芽孢杆菌13002的益生特性[J]. 食品工业与科技,2018,39(6):97−101. [JIN X, LIU D M, RUAN H, et al. Probiotic properties of Bacillus coagulans 13002[J]. Science and Technology of Food Industry,2018,39(6):97−101. JIN X, LIU D M, RUAN H, et al. Probiotic properties of Bacillus coagulans 13002[J]. Science and Technology of Food Industry, 2018, 39(6): 97-101.
[28]	GöZDE K, ZERRIN E. Potential use of Bacillus coagulans in the food industry[J]. Foods,2018,7(92):1−10.
[29]	黄遵锡, 程志斌. 饲粮添加凝结芽孢杆菌与枯草芽孢杆菌对断奶仔猪生长性能的影响[J]. 饲料工业,2017,38(21):1−6. [HUANG Z X, CHENG Z B. Effects of Bacillus coagulans and Bacillus subtilis on the growth performance in weaned piglets[J]. Feed Industry,2017,38(21):1−6. HUANG Z X, CHENG Z B. Effects of Bacillus coagulans and Bacillus subtilis on the growth performance in weaned piglets[J]. Feed Industry, 2017, 38(21): 1-6.
[30]	ZHEN W, SHAO Y, GONG X, et al. Effect of dietary Bacillus coagulans supplementation on growth performance and immune responses of broiler chickens challenged by Salmonella enteritidis[J]. Poult Sci,2018,97(8):2654−2666. doi: 10.3382/ps/pey119
[31]	黄遵锡, 程志斌. 饲粮不同添加量的凝结芽孢杆菌对仔猪生长性能及肠道健康的影响[J]. 饲料工业,2020,41(15):1−7. [HUANG Z X, CHENG Z B. Effects of dietary supplementation of probiotic Bacillus coagulans at various dosages on the growth performance and gut health in weanling piglets[J]. Feed Industry,2020,41(15):1−7. HUANG Z X, CHENG Z B. Effects of dietary supplementation of probiotic Bacillus coagulans at various dosages on the growth performance and gut health in weanling piglets[J]. Feed Industry, 2020, 41(15): 1-7.
[32]	陈宇, 刘水灵, 高欢, 等. 凝结芽孢杆菌的生物学特性及其在畜禽生产中的应用[J]. 当代畜牧,2020(5):6−9. [CHEN Y, LIU S L, GAO H, et al. Biological characteristics of Bacillus coagulans and its application in livestock production[J]. Contemporary Animal Husbandry,2020(5):6−9. CHEN Y, LIU S L, GAO H, et al. Biological characteristics of Bacillus coagulans and its application in livestock production[J]. Contemporary Animal Husbandry, 2020(5): 6-9.
[33]	FU L, WANG C, RUAN X, et al. Preservation of large yellow croaker (Pseudosciaena crocea) by coagulin L1208, a novel bacteriocin produced by Bacillus coagulans L1208[J]. Int J Food Microbiol,2018,266:60−68. doi: 10.1016/j.ijfoodmicro.2017.11.012
[34]	柳成东, 肖明霞, 李琦华, 等. 饲用益生菌凝结芽孢杆菌抑菌性能的研究[J]. 中国畜牧杂志,2021,57(10):236−241. [LIU C D, XIAO M X, LI Q H, et al. Study on the antibacterial activity properties of the feed probiotic Bacillus coagulans[J]. Chinese Journal of Animal Science,2021,57(10):236−241. LIU C D, XIAO M X, LI Q H, et al. Study on the antibacterial activity properties of the feed probiotic Bacillus coagulans[J]. Chinese Journal of Animal Science, 2021, 57(10): 236-241.
[35]	Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; twenty-second informational supplement[M]. CLSI document M100-S22. Wayne Pennsylvania: Clinical and Laboratory Standards Institute, 2013: 64−65.
[36]	MU Y, CONG Y. Bacillus coagulans and its applications in medicine[J]. Beneficial Microbes,2019,10(6):1−10.

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