Research on the Anti-aging Effect of <i>Rosa xanthina</i> Lindl Fruits Based on <sup>1</sup>H NMR Metabolomics

NIU Dan; CHEN Lei; REN Jing; WANG Jindong

doi:10.13386/j.issn1002-0306.2022080287

Volume 44 Issue 12

Jun. 2023

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Science and Technology of Food Industry > 2023 > 44(12): 10-17. > DOI: 10.13386/j.issn1002-0306.2022080287

NIU Dan, CHEN Lei, REN Jing, et al. Research on the Anti-aging Effect of Rosa xanthina Lindl Fruits Based on ¹H NMR Metabolomics[J]. Science and Technology of Food Industry, 2023, 44(12): 10−17. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080287.

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Research on the Anti-aging Effect of Rosa xanthina Lindl Fruits Based on ¹H NMR Metabolomics

1.
Shanxi Provincial Drug Evaluation Center (Shanxi Institute of Medicine and Life Sciences), Taiyuan 030006, China
2.
Center for Drug Inspection of Shanxi Province (Center for Vaccine Inspection of Shanxi Province), Taiyuan 030031, China

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Received Date: August 29, 2022
Available Online: April 19, 2023

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Abstract

Abstract

Objective: The anti-aging effect of the fruits of Rosa xanthina Lindl (RF) was investigated by the ¹H NMR based metabolomics using D-galactose-induced aging rats. Methods: Rat subacute senile model was established by subcutaneous injection of D-galactose (400 mg/kg), and the anti-aging effects of RF were studied at three different doses (1.6, 3.2, 6.4 g/kg). On the 30th day, rat serum was collected and the serum superoxide dismutase (SOD), lipid peroxide (LPO) and hydroxyproline (Hyp) were determined. The anti-aging effects of high, middle, low dose of RF were investigated by ¹H NMR metabolomics, and combined with multivariate statistical analysis. Results: When compared with the model group, RF extracts could increase serum SOD and Hyp levels (P<0.01) and decrease LPO level (P<0.05, P<0.01) significantly. For the rats in the model group, 13 differential metabolites in serum were changed (P<0.05, P<0.01) significantly. After treatment by RF, seven metabolites including lipid, arginine, glycerol, trimethylamine N-Oxide (TMAO), glycine, alanine and N-acetyl glycoprotein were regulated in the high dose group, and then three metabolites including TMAO, N-acetyl glycoprotein and leucine were altered in the middle dose group, while only phosphatidylcholine (PC) was decreased in the low dose group (P<0.05, P<0.01) significantly. The best ranking of Efficacy index (EI) in three groups was high, middle and low, indicating that the high dose of RF showed the best anti-aging effect. Conclusion: RF exhibited the anti-aging effect probably through regulating oxidative stress, promoting collagen synthesis, and also related with various metabolic pathways, such as amino acid metabolism and gut microbiota metabolism.
- Rosa xanthina Lindl fruits extract,
- metabolomics,
- anti-aging,
- D-galactose

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