<i>Poria cocos</i> Polysaccharides Regulate Anxiety and Depression-like Behaviors Induced by Lipopolysaccharide through NF-<i>κ</i>B and NLRP3 Signaling Pathways

SHI Yunjing; LI Yuxia

doi:10.13386/j.issn1002-0306.2022080276

Volume 44 Issue 12

Jun. 2023

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Science and Technology of Food Industry > 2023 > 44(12): 371-377. > DOI: 10.13386/j.issn1002-0306.2022080276

SHI Yunjing, LI Yuxia. Poria cocos Polysaccharides Regulate Anxiety and Depression-like Behaviors Induced by Lipopolysaccharide through NF-κB and NLRP3 Signaling Pathways[J]. Science and Technology of Food Industry, 2023, 44(12): 371−377. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080276.

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Poria cocos Polysaccharides Regulate Anxiety and Depression-like Behaviors Induced by Lipopolysaccharide through NF-κB and NLRP3 Signaling Pathways

SHI Yunjing^1,,
LI Yuxia^2, ,

1.
Hebei University of Chinese Medicine, Shijiazhuang 050020, China
2.
Luohe Medical College, Luohe 462002, China

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Received Date: August 29, 2022
Available Online: April 10, 2023

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Abstract

Abstract

Objective: To explore the effect of Poria cocos polysaccharides (Poria cocos polysaccharide, PPS) on anxiety and depression-like behaviors induced by lipopolysaccharide (LPS) and analyze its mechanism. Methods: BV-2 cells were divided into control group, LPS group, LPS+fluoxetine group and LPS+PPS (PPS of 4, 8, 16 μmol/L, respectively). After 24 h treatment, the level of intracellular reactive oxygen species (ROS) was detected by dichlorodihydrofluorescein dihydroacetate (DCFH-DA) fluorescence probe, the level of NO in culture supernatant was detected by griess method, and the levels of TNF-α and IL-1β in cell supernatant were detected by enzyme linked immunosorbent assay (ELISA). The levels of CD206, CD16/32 and NF-κB p65 were detected by western blot. In animal experiment, mice were divided into control group, model group, LPS+fluoxetine group, LPS+PPS low dose group (20 mg/kg) and LPS+PPS high dose group (80 mg/kg). Depression-like behavior test was performed, and the concentration of TNF-α, IL-1β and IL-18 in hippocampus was detected. The protein levels of CD206, CD16/32, NF-κB p65, NLRP3, ASC and Cleaved caspase-1 in hippocampus were analyzed. Results: Compared with LPS group, 4,8,16 μmol/L PPS significantly decreased the relative fluorescence intensity of ROS (P<0.01), decreased the levels of NO, TNF-α and IL-1β (P<0.01), decreased the levels of CD16/32 and NF-κB p65 (P<0.05), and increased the level of CD206 (P<0.05). Compared with the model group, the preference rate, crossing times and modification times in LPS+PPS low dose group and high dose LPS+PPS group significantly increased (P<0.01), while the tail suspension time and forced swimming immobility time significantly decreased (P<0.01). Compared with the model group, the levels of IL-1β, IL-18 and TNF-α in LPS+PPS low dose group and LPS+PPS high dose group significantly decreased (P<0.01), CD16/32 and NF-κB p65 level were significantly down-regulated, CD206 was significantly up-regulated, and the levels of NLRP3, ASC and Cleaved caspase-1 significantly decreased. Conclusion: Poria cocos polysaccharide can alleviate LPS-induced anxiety and depression-like behavior by inhibiting NF-κB and NLRP3 signal pathways.
- Poria cocos polysaccharide,
- NF-κB signal pathway,
- NLRP3 signal pathway,
- lipopolysaccharide,
- anxiety,
- depression-like behavior

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References

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