Construction of Heterotrophic Axenic System of <i>Chlorella sorokiniana</i> Based on Antibiotic Sensitivity

CAI Weixin; LIU Xinyu; MA Ruijuan; CHEN Jianfeng; XIE Youping

doi:10.13386/j.issn1002-0306.2022080251

Volume 44 Issue 12

Jun. 2023

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Science and Technology of Food Industry > 2023 > 44(12): 148-156. > DOI: 10.13386/j.issn1002-0306.2022080251

CAI Weixin, LIU Xinyu, MA Ruijuan, et al. Construction of Heterotrophic Axenic System of Chlorella sorokiniana Based on Antibiotic Sensitivity[J]. Science and Technology of Food Industry, 2023, 44(12): 148−156. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080251.

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Construction of Heterotrophic Axenic System of Chlorella sorokiniana Based on Antibiotic Sensitivity

Marine Biological Manufacturing Center of Fuzhou Institute of Oceanography, Fuzhou University, Fuzhou 350108, China

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Received Date: August 23, 2022
Available Online: April 19, 2023

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Abstract

Abstract

To construct a heterotrophic axenic system of Chlorella sorokiniana FZU60, the symbiotic bacteria were isolated and identified, and the effects of eight common antibiotics (cefotaxime, kanamycin, neomycin, ampicillin, streptomycin, tetracycline, chloramphenicol, and erythromycin) on the cell growth of symbiotic bacteria and C. sorokiniana FZU60 were investigated. Results showed that the main symbiotic bacteria were Bacillus (78.76%) and Methylobacterium (3.14%). Besides, 10 mg/L cefotaxime, 10 mg/L tetracycline, 100 mg/L kanamycin, and 100 mg/L erythromycin showed excellent inhibitory effect on these two symbiotic bacteria but no obvious effect on the cell growth of C. sorokiniana FZU60. Based on the above-mentioned results, the inhibition effect of different antibiotic combinations on symbiotic bacteria of C. sorokiniana FZU60 was further investigated. It was found that the heterotrophic axenic system could be successfully constructed by using the combination of 10 mg/L cefotaxime, 10 mg/L tetracycline, and 100 mg/L kanamycin. Moreover, C. sorokiniana FZU60 could maintain stable and axenic growth after three generations. The experimental results would provide theoretical reference and experimental basis for the preparation of axenic microalgal seed for high-cell-density heterotrophic cultivation of C. sorokiniana.
- Chlorella sorokiniana,
- heterotrophic cultivation,
- antibiotic,
- axenic cultivation

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