HU Hangyu, YAO Mengke, SUN Ting, et al. Improvement Effect of the EPS Produced by Long Shelf-life Yogurt Culture on DSS-Induced Enteritis[J]. Science and Technology of Food Industry, 2023, 44(12): 378−387. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080243.
Citation: HU Hangyu, YAO Mengke, SUN Ting, et al. Improvement Effect of the EPS Produced by Long Shelf-life Yogurt Culture on DSS-Induced Enteritis[J]. Science and Technology of Food Industry, 2023, 44(12): 378−387. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080243.

Improvement Effect of the EPS Produced by Long Shelf-life Yogurt Culture on DSS-Induced Enteritis

More Information
  • Received Date: August 22, 2022
  • Available Online: April 10, 2023
  • In order to study the relieving effect of extracellular polysaccharides (EPS-A,EPS-B) which were extracted and purified from long shelf-life yogurt fermented by long shelf-life yogurt culture A and culture B, their molecular characteristics, structural morphology and monosaccharide composition were determined, and the mouse weight, influence of colon histopathology, the expression of inflammatory factors, the content of myeloperoxidase (MPO) and tight junction proteins (ZO-1, Occludin) in mice were also analyzed through constructing an animal model of colonic inflammation in mice by dextran sulfate sodium (DSS). The results showed that there were significantly differences on the molecular morphology and monosaccharide compositions between EPS-A and EPS-B. The EPS-A was slightly loose in structure, composed of three monosaccharides (galactosamine hydrochloride, galactose and glucose) with a molar ratio of 0.345:0.21:0.435. The structure of EPS-B was compact and it composed of four monosaccharides (galactosamine hydrochloride, glucosamine hydrochloride, galactose and glucose) with a molar ratio of 0.421:0.05:0.207:0.322. The weight of IBD mice was recovered in all the exopolysaccharide treatment groups, but there was no significant decrease in the disease activity index (DAI) score. The colon morphology of the high dose group of EPS-A was close to that of the blank control group, and there was no significant difference in colon length and colon coefficient between the two groups (P>0.05), the same below). Combined with the expression of inflammatory factor (TNF-α, IL-1β, IL-10) in different treatment groups, the high dose group of EPS-A had the best response to those inflammatory factors, and there was no significant difference between it and the drug treatment group (P>0.05). The expression of ZO-1 and occludin protein in the high-dose and low-dose groups of EPS-A was significantly improved and same as the drug treatment group (P<0.05). The expression of ZO-1 protein in the high-dose group of EPS-B was also significantly higher than that in the model group, but the effect was not as good as that in the EPS-A group (P<0.05). There was no significant improvement in reducing MPO activity in different extracellular polysaccharide treatment groups compared with the model group (P>0.05). In summary, it could be seen that EPS-A can significantly improve the enteritis of mice, and its effect was better than that of EPS-B.
  • [1]
    SULTAN S, EL-MOWAFY M, ELGAML A, et al. Metabolic influences of gut microbiota dysbiosis on inflammatory bowel disease[J]. Front Physiol,2021,12:715506. doi: 10.3389/fphys.2021.715506
    [2]
    JEONG D Y, KIM S, SON M J, et al. Induction and maintenance treatment of inflammatory bowel disease: A comprehensive review[J]. Autoimmun Rev,2019,18(5):439−454. doi: 10.1016/j.autrev.2019.03.002
    [3]
    陈国伟, 邱春红, 田灵敏, 等. 食源性天然产物中多糖干预炎症性肠病的研究进展[J]. 食品科学,2019,40(13):281−287. [CHEN G W, QIU C H, TIAN L M, et al. Recent progress in food-derived natural polysaccharideintervention in inflammatory bowel disease[J]. Food Science,2019,40(13):281−287. doi: 10.7506/spkx1002-6630-20180619-371

    CHEN G W, QIU C H, TIAN L M, et al. Recent progress in food-derived natural polysaccharideintervention in inflammatory bowel disease[J]. Food Science, 2019, 40(13): 281-287. doi: 10.7506/spkx1002-6630-20180619-371
    [4]
    ROUND J L, LEE S M, LI J, et al. The toll-like receptor 2 pathway establishes colonization by a commensal of the human microbiota[J]. Science,2011,332(6032):974−977. doi: 10.1126/science.1206095
    [5]
    HAYASHI A, SATO T, KAMADA N, et al. A single strain of Clostridium butyricum induces intestinal IL-10- producing macrophages to suppress acute experimental colitis in mice[J]. Cell Host Microbe,2013,13(6):711−722. doi: 10.1016/j.chom.2013.05.013
    [6]
    胡丹丹. 植物乳杆菌LP17-1对小鼠结肠炎缓解作用的研究及其胞外多糖分离提取[D]. 长沙: 中南林业科技大学, 2021

    HU D D. Study on the alleviating effect of Lactobacillus plantarum LP17-1 on colitis in miceand the isolation and extraction of extracellular[D]. Changsha: Central South University of Forestry and Technology, 2021.
    [7]
    JIANG Y, YANG Z. A functional and genetic overview of exopolysaccharides produced by Lactobacillus plantarum[J]. Journal of Functional Foods,2018,47:229−240. doi: 10.1016/j.jff.2018.05.060
    [8]
    MIN Z, XIAONA H, AZIZ T, et al. Exopolysaccharides from Lactobacillus plantarum YW11 improve immune response and ameliorate inflammatory bowel disease symptoms[J]. Acta Biochim Pol,2020,67(4):485−493.
    [9]
    ZHOU X, QI W, HONG T, et al. Exopolysaccharides from Lactobacillus plantarum NCU116 regulate intestinal barrier function via STAT3 signaling pathway[J]. J Agric Food Chem,2018,66(37):9719−9727. doi: 10.1021/acs.jafc.8b03340
    [10]
    伍素芳. 常温饮用型酸奶生产工艺的优化[J]. 饮料工业,2020,23(6):62−66. [WU S F. Optimization ofproduction process for long life drinking yoghurt[J]. Beverage Industry,2020,23(6):62−66.

    WU S F. Optimization ofproduction process for long life drinking yoghurt[J]. Beverage Industry, 2020, 23(6): 62-66.
    [11]
    WANG J, ZHAO X, TIAN Z, et al. Characterization of an exopolysaccharide produced by Lactobacillus plantarum YW11 isolated from Tibet Kefir[J]. Carbohydr Polym,2015,125:16−25. doi: 10.1016/j.carbpol.2015.03.003
    [12]
    WANG Y, LI C, LIU P, et al. Physical characterization of exopolysaccharide produced by Lactobacillus plantarum KF5 isolated from Tibet Kefir[J]. Carbohydrate Polymers,2010,82(3):895−903. doi: 10.1016/j.carbpol.2010.06.013
    [13]
    LI W, JI J, CHEN X, et al. Structural elucidation and antioxidant activities of exopolysaccharides from Lactobacillus helveticus MB2-1[J]. Carbohydr Polym,2014,102:351−359. doi: 10.1016/j.carbpol.2013.11.053
    [14]
    吴淑娟, 王新军, 张武胜, 等. 石榴汁对DSS诱导的小鼠溃疡性结肠炎的作用[J/OL]. 食品科学: 1−13[2023-04-26]. http://kns.cnki.net/kcms/detail/11.2206.TS.20220915.1817.022.html.

    WU S J, WANG X J, ZHANG W S, et al. Effects of pomegranate juice on DSS-induced ulcerative colitis inmice[J/OL]. Food Science, 2022: [2023-04-26]. http://kns.cnki.net/kcms/detail/11.2206.TS.20220915.1817.022.html.
    [15]
    JANG Y J, KIM W K, HAN D H, et al. Lactobacillus fermentum species ameliorate dextran sulfate sodium-induced colitis by regulating the immune response and altering gut microbiota[J]. Gut Microbes,2019,10(6):696−711. doi: 10.1080/19490976.2019.1589281
    [16]
    许良中, 杨文涛. 免疫组织化学反应结果的判断标准[J]. 中国癌症杂志, 1996, 6(4): 229-231

    XU LZ, YANG W T. Judgment criteria of immunohistochemical reaction results[J].China Oncology, 1996, 6(4): 229-231.
    [17]
    ALE E C, PEREZLINDO M J, PAVON Y, et al. Technological, rheological and sensory characterizations of a yogurt containing an exopolysaccharide extract from Lactobacillus fermentum Lf2, a new food additive[J]. Food Res Int,2016,90:259−267. doi: 10.1016/j.foodres.2016.10.045
    [18]
    LI C, LI W, CHEN X, et al. Microbiological, physicochemical and rheological properties of fermented soymilk produced with exopolysaccharide (EPS) producing lactic acid bacteria strains[J]. LWT-Food Science and Technology,2014,57(2):477−485. doi: 10.1016/j.lwt.2014.02.025
    [19]
    SHUHONG Y, MEIPING Z, HONG Y, et al. Biosorption of Cu2+, Pb2+ and Cr6+ by a novel exopolysaccharide fromArthrobacter ps-5[J]. Carbohydr Polym,2014,101:50−56. doi: 10.1016/j.carbpol.2013.09.021
    [20]
    DU R, YU L, YU N, et al. Characterization of exopolysaccharide produced by Levilactobacillus brevis HDE-9 and evaluation of its potential use in dairy products[J]. Int J Biol Macromol,2022,217:303−311. doi: 10.1016/j.ijbiomac.2022.07.057
    [21]
    KAVITA K, MISHRA A, JHA B. Extracellular polymeric substances from two biofilm forming Vibrio species: Characterization and applications[J]. Carbohydr Polym,2013,94(2):882−888. doi: 10.1016/j.carbpol.2013.02.010
    [22]
    SHINGEL K I. Determination of structural peculiarities of dexran, pullulan and γ-irradiated pullulan by Fourier-transform IR spectroscopy[J]. Carbohydrate Research,2002,337(16):1445−1451. doi: 10.1016/S0008-6215(02)00209-4
    [23]
    İSPIRLI H, SAGDIC O, YıLMAZ M T, et al. Physicochemical characterisation of an α-glucan from Lactobacillus reuteri E81 as a potential exopolysaccharide suitable for food applications[J]. Process Biochemistry,2019,79:91−96. doi: 10.1016/j.procbio.2018.12.015
    [24]
    MIAO M, MA Y, HUANG C, et al. homopolysaccharide from Lactobacillus reuteri SK24.003[J]. Carbohydr Polym,2015,131:377−383. doi: 10.1016/j.carbpol.2015.05.066
    [25]
    曹明泽, 王旭荣, 王磊, 等. 小鼠溃疡性结肠炎模型的建立与评价[J]. 中国畜牧兽医,2016,43(1):171−175. [[CAO M Z, WANG X R, WANG L, et al. Physicochemical properties of a water soluble extracellular[J]. China Animal Husbandry & Veterinary Medicine,2016,43(1):171−175.

    [CAO M Z, WANG X R, WANG L, et al. Physicochemical properties of a water soluble extracellular [J]. ChinaAnimal Husbandry &Veterinary Medicine, 2016, 43(1): 171-175.
    [26]
    ZHANG Z, LI S, CAO H, et al. The protective role of phloretin against dextran sulfate sodium-induced ulcerative colitis in mice[J]. Food Funct,2019,10(1):422−431. doi: 10.1039/C8FO01699B
    [27]
    LI B, ALLI R, VOGEL P, et al. IL-10 modulates DSS-induced colitis through a macrophage-ROS-NO axis[J]. Mucosal Immunol,2014,7(4):869−878. doi: 10.1038/mi.2013.103
    [28]
    SOUFLI I, TOUMI R, RAFA H, et al. Overview of cytokines and nitric oxide involvement in immuno- pathogenesis of inflammatory bowel diseases[J]. World J Gastrointest Pharmacol Ther,2016,7(3):353−360. doi: 10.4292/wjgpt.v7.i3.353
    [29]
    POPIVANOVA B K, KITAMURA K, WU Y, et al. Blocking TNF-alpha in mice reduces colorectal carcinogenesis associated with chronic colitis[J]. J Clin Invest,2008,118(2):560−570.
    [30]
    TUMMALA P, ROOKE M, DAHLSTROM J E, et al. Glutathione transferase omega 1 confers protection against azoxymethane-induced colorectal tumourformation[J]. Carcinogenesis,2021,42(6):853−863.
    [31]
    李玥, 钱家鸣. 双歧杆菌对葡聚糖硫酸钠(DSS)诱导的小鼠结肠炎肠道黏膜的保护作用及其对NF-κB的影响[J]. 现代消化及介入诊疗,2010,15(3):131−135. [LI Y, QIAN J M. The probiotics, bifidobacterium, ameliorates dextran sulfate sodium induced colitis in mice[J]. Modern Digestion & Intervention,2010,15(3):131−135.

    LI Y, QIAN J M. The probiotics, bifidobacterium, ameliorates dextran sulfate sodium induced colitis in mice[J]. Modern Digestion & Intervention, 2010, 15(3): 131-135.
    [32]
    EOM T, KIM Y S, CHOI C H, et al. Current understanding of microbiota- and dietary-therapies for treating inflammatory bowel disease[J]. J Microbiol,2018,56(3):189−198. doi: 10.1007/s12275-018-8049-8
    [33]
    ARIKE L, HOLMEN-LARSSON J, HANSSON G C. Intestinal Muc2 mucin O-glycosylation is affected by microbiota and regulated by differential expression of glycosyltranferases[J]. Glycobiology,2017,27(4):318−328.
    [34]
    徐婧, 徐豪明, 周有连, 等. 抗生素清肠对丁酸梭菌保护肠炎小鼠肠黏膜屏障的影响及初步机制研究[J]. 现代消化及介入诊疗,2021,26(2):208−213,221. [XU J, XU H M, ZHOU Y L, et al. Effect ofantibiotic cocktail on the intestinal mucosal barrier of mice with colitis protected by Clostridium butyricum[J]. Modern Digestion & Intervention,2021,26(2):208−213,221.

    XU J, XU H M, ZHOU Y L, et al. Effect ofantibiotic cocktail on the intestinal mucosal barrier of mice with colitis protected by Clostridium butyricum[J]Modern Digestion & Intervention, 2021, 26(2): 208-213+221.
    [35]
    FERREIRA S S, PASSOS C P, MADUREIRA P, et al. Structure-function relationships of immunostimulatory polysaccharides: A review[J]. Carbohydrate Polymers,2015,132:378−396. doi: 10.1016/j.carbpol.2015.05.079
    [36]
    刘袆帆, 郭烁璇, 林映妤, 等. 石斛多糖的构效关系研究进展[J]. 现代食品科技,2021,37(1):308−338. [LIUH F, GUO S X, LIN Y S, et al. The relationship between the structure and function of dendrobium polysaccharides: A review[J]. Modern Food Science and Technology,2021,37(1):308−338.

    LIUH F, GUO S X, LIN Y S, et al. The relationship between the structure and function of dendrobium polysaccharides: A review[J]. Modern Food Science and Technology, 2021, 37(1): 308-338.
    [37]
    KANG H, CHOI H S, KIM J E, et al. Exopolysaccharide-overproducing Lactobacillus paracasei KB28 induces cytokines in mouse peritoneal macrophages via modulation of NF-kappabeta and MAPKs[J]. J Microbiol Biotechnol,2011,21(11):1174−1178. doi: 10.4014/jmb.1105.05026
  • Cited by

    Periodical cited type(2)

    1. 常旭龙,周青霞,马文聪,詹圳铭,姚欣鑫,周爱梅. 不同发酵剂发酵酸奶的风味及理化特性的研究. 食品工业科技. 2025(08): 263-271 . 本站查看
    2. 徐畅,刘天一,刘文佳,张俐敏,莫继先. 微生物胞外多糖的来源、生物合成及功能研究进展. 生物技术进展. 2024(03): 368-376 .

    Other cited types(1)

Catalog

    Article Metrics

    Article views (146) PDF downloads (8) Cited by(3)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return