Formulation Optimization and Functional Evaluation of Immunomodulatory Teabags

LIU Haixin; LI Wei; QIAO Yashi; CAO Yuwang; ZHANG Boju; LIU Caixia

doi:10.13386/j.issn1002-0306.2022080176

Volume 44 Issue 12

Jun. 2023

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Science and Technology of Food Industry > 2023 > 44(12): 388-396. > DOI: 10.13386/j.issn1002-0306.2022080176

LIU Haixin, LI Wei, QIAO Yashi, et al. Formulation Optimization and Functional Evaluation of Immunomodulatory Teabags[J]. Science and Technology of Food Industry, 2023, 44(12): 388−396. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080176.

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Formulation Optimization and Functional Evaluation of Immunomodulatory Teabags

College of Traditional Chinese Medicine and Food Engineering, Shanxi University of Traditional Chinese Medicine, Jinzhong 030619, China

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Received Date: August 17, 2022
Available Online: April 17, 2023

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Abstract

Objective: The aim of this study was to develop a functional teabags for enhancing immunity with green tea, Astragali radix, Codonopsis radix, Lycii fructus and Jujubae fructus. Methods: Sensory evaluation was used as indicator, the best formula of the teabags was optimized by orthogonal test combined with fuzzy mathematics comprehensive evaluation method. And the total polysaccharide content was detected by the phenol-sulfuric acid method. SPF-grade Kunming mice were randomly divided into blank control group, model control group, positive control group and teabags group with low-, medium- and high- dose (0.52, 1.04, 1.56 g/kg BW). Except for the blank control group, the immunosuppressive model was prepared by intraperitoneally injected with cyclophosphamidein in all groups, and the body weight and organ index of mice were measured after 21 days of continuous administration. After those processes, CD3⁺ cells, CD4⁺ cells, CD8⁺ cells and CD4⁺CD8⁺ cells were detected by flow cytometry in the spleen of mice. In addition, the level of interferon-γ (IFN-γ) in mouse serum was detected by ELISA. Results: In the optimal formulation of the teabags, the amounts of Astragali radix, Codonopsis radix, Lycii fructus, Jujubae fructus and green tea were 13.00%, 26.30%, 20.50%, 20.50% and 19.70% of the total mass of the teabags contents, respectively. In addition, the content of polysaccharide was 33.84±0.52 mg/g. The efficacy test results showed that compared with the blank control group, the body weight and thymus index of mice in the model control group were significantly declined (P<0.05), CD3⁺ cells, CD4⁺ cells, CD8⁺ cells and CD4⁺CD8⁺ cells ratio were significantly reduced (P<0.05), and IFN-γ content in serum significantly decreased (P<0.05), which indicated that the immunosuppressive model was established successfully. Compared with the model control group, the body weight, thymus index, CD3⁺ cells, CD4⁺ cells, CD8⁺ cells and CD4⁺CD8⁺ cells ratio, and the serum IFN-γ content of mice in the low dose group significantly increased (P<0.05). The thymus index, CD4⁺ cells, CD8⁺ cells and CD4⁺CD8⁺ cells ratio of mice in the medium dose group significantly increased (P<0.05). Additionally, the thymus index of mice in the high dose group significantly increased (P<0.05). Overall, the immunomodulation effect of the teabags in the low-dose group was better than that of the medium- and high-dose group. Conclusion: Immunomodulatory teabags had an immune-enhancing effect, and its effect is related to the regulation of the T cell immune mechanism.
- green tea,
- Astragali radix,
- Codonopsis radix,
- formulation optimization,
- sensory evaluation,
- polysaccharide,
- immunomodulation

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[1]	WU S W, XUAN Z Z, LI F, et al. Work-recreation balance, health-promoting lifestyles and suboptimal health status in southern China: A cross-sectional study[J]. International Journal of Environmental Research and Public Health,2016,13(3):339−355. doi: 10.3390/ijerph13030339
[2]	MA H D, DENG Y R, TIAN Z G, et al. Traditional Chinese medicine and immune regulation[J]. Clinical Reviews in Allergy & Immunology,2013,44(3):229−241.
[3]	罗江琼, 石雨, 罗理勇, 等. 茶与药茶保健功效的研究进展[J]. 保健医学研究与实践,2021,18(5):170−176. [LUO J Q, SHI Y, LUO L Y, et al. Research progreson the health benefits oftea and herbal tea[J]. Health Medicine Research and Practice,2021,18(5):170−176. LUO J Q, SHI Y, LUO L Y, et al. Research progreson the health benefits oftea and herbal tea[J]. Health Medicine Research and Practice, 2021, 18(5): 170-176.
[4]	李辉. 关于山西药茶产业现状及发展探讨[J]. 食品安全导刊,2021(32):186−188. [LI H. Discussion on present situation and development of Shanxi medicinal tea industry[J]. China Food Safety Magazine,2021(32):186−188. doi: 10.3969/j.issn.1674-0270.2021.32.spaqdk202132077 LI H. Discussion on present situation and development of Shanxi medicinal tea industry[J]. China Food Safety Magazine, 2021(32): 186-188. doi: 10.3969/j.issn.1674-0270.2021.32.spaqdk202132077
[5]	王超楠, 程东岩, 王健, 等. 黄芪及复方黄芪制剂双向免疫调节作用研究进展[J]. 中华中医药学刊,2021,39(5):126−129. [WANG C N, CHENG D Y, WANG J, et al. Research progress on bidirectional immunoregulation of Huangqi (Astragali radix) and its compound preparations[J]. Chinese Archives of Traditional Chinese Medicine,2021,39(5):126−129. WANG C N, CHENG D Y, WANG J, et al. Research progress on bidirectional immunoregulation of Huangqi (Astragali radix) and its compound preparations[J]. Chinese Archives of Traditional Chinese Medicine, 2021, 39(5): 126-129.
[6]	ELABD H, WANG H P, SHAHEEN A, et al. Huangqi (Astragali radix) nanoparticles markedly improve immune and antioxidative responses; and protection against Aeromonas veronii in Nile tilapia Oreochromis niloticus[J]. Fish and Shellfish Immunology,2020,97:248−256. doi: 10.1016/j.fsi.2019.12.025
[7]	陈冬梅, 蒙洁, 刘佳佳, 等. 基于网络药理学的党参增强免疫功能机制研究[J]. 中华中医药学刊,2020,38(2):184−187. [CHEN D M, MENG J, LIU J J, et al. Network pharmacology-based study on mechanism of Codonopsis pilosula enhancing immune function[J]. Archives of Traditional Chinese Medicine,2020,38(2):184−187. CHEN D M, MENG J, LIU J J, et al. Network pharmacology-based study on mechanism of Codonopsis pilosula enhancing immune function[J]. Archives of Traditional Chinese Medicine, 2020, 38(2): 184-187.
[8]	孙正霄, 肖顺丽, 刘陆, 等. 枸杞咀嚼片免疫调节及抗氧化活性分析[J]. 中国实验方剂学杂志,2022,28(8):46−53. [SUN Z X, XIAO S L, LIU L, et al. Immunomodulatory and antioxidant activity of Gouqi chewable tablets[J]. Chinese Journal of Experimental Traditional Medical Formulae,2022,28(8):46−53. SUN Z X, XIAO S L, LIU L, et al. Immunomodulatory and antioxidant activity of Gouqi chewable tablets[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2022, 28(8): 46-53.
[9]	王超, 王一民, 郭飞, 等. 大枣多糖对CFS大鼠的免疫调节作用[J]. 西北大学学报(自然科学版),2015,45(1):73−78. [WANG C, WANG Y M, GUO F, et al. Immune function of Ziziphus jujube polysaccharides on chronic fatigue syndrome rats[J]. Journal of Northwest University (Natural Science Edition),2015,45(1):73−78. WANG C, WANG Y M, GUO F, et al. Immune function of Ziziphus jujube polysaccharides on chronic fatigue syndrome rats[J]. Journal of Northwest University (Natural Science Edition), 2015, 45(1): 73-78.
[10]	戴瑜婷, 张雪燕, 王艺璇, 等. 黄芪的现代研究进展及其质量标志物的预测分析[J]. 中国中药杂志,2022,47(7):1754−1764. [DAI Y T, ZHANG X Y, WANG Y X, et al. Research progress on Astragali radix and prediction of its quality markers (Q-markers)[J]. China Journal of Chinese Materia Medica,2022,47(7):1754−1764. DAI Y T, ZHANG X Y, WANG Y X, et al. Research progress on Astragali radix and prediction of its quality markers (Q-markers)[J]. China Journal of Chinese Materia Medica, 2022, 47(7): 1754-1764.
[11]	边惠琴, 武晓玉, 夏鹏飞, 等. 党参的研究进展及质量标志物的预测分析[J]. 华西药学杂志,2022,37(3):337−344. [BIAN H Q, WU X Y, XIA P F, et al. Research progress of Codonopsis radix and predictive analysis on quality markers[J]. West China Journal of Pharmaceutical Sciences,2022,37(3):337−344. BIAN H Q, WU X Y, XIA P F, et al. Research progress of Codonopsis radix and predictive analysis on quality markers[J]. West China Journal of Pharmaceutical Sciences, 2022, 37(3): 337-344.
[12]	宋艳梅, 张启立, 崔治家, 等. 枸杞子化学成分和药理作用的研究进展及质量标志物的预测分析[J]. 华西药学杂志,2022,37(2):206−213. [SONG Y M, ZHANG Q L, CUI Z J, et al. Research progress on chemical constituents and pharmacological effects of lyciifructus and its quality markers prediction and analysis[J]. West China Journal of Pharmaceutical Sciences,2022,37(2):206−213. SONG Y M, ZHANG Q L, CUI Z J, et al. Research progress on chemical constituents and pharmacological effects of lyciifructus and its quality markers prediction and analysis[J]. West China Journal of Pharmaceutical Sciences, 2022, 37(2): 206-213.
[13]	ZHU W, ZHOU S, LIU J, et al. Prebiotic, immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide[J]. Biomedicine & Pharmacotherapy,2019,121:109591.
[14]	WANG W, LI X, CHEN K, et al. Extraction optimization, characterization and immunity activity of polysaccharides from Fructus jujubae[J]. International Journal of Biological Macromolecules,2020,160:380−389. doi: 10.1016/j.ijbiomac.2020.05.158
[15]	陆建良, 梁月荣, 龚淑英, 等. 茶汤色差与茶叶感官品质相关性研究[J]. 茶叶科学,2002,22(1):57−61. [LU J L, LIANG Y R, GONG S Y, et al. Studies on relationship between liquor chromaticity and organoleptic quality of tea[J]. Journal of Tea Science,2002,22(1):57−61. doi: 10.3969/j.issn.1000-369X.2002.01.012 LU J L, LIANG Y R, GONG S Y, et al. Studies on relationship between liquor chromaticity and organoleptic quality of tea[J]. Journal of Tea Science, 2002, 22(1): 57-61. doi: 10.3969/j.issn.1000-369X.2002.01.012
[16]	李玉珍, 肖怀秋. 模糊数学评价法在食品感官评价中的应用[J]. 中国酿造,2016,35(5):16−19. [LI Y Z, XIAO H Q. Application of fuzzy mathematics evaluation method in food sensory evaluation[J]. China Brewing,2016,35(5):16−19. doi: 10.11882/j.issn.0254-5071.2016.05.004 LI Y Z, XIAO H Q. Application of fuzzy mathematics evaluation method in food sensory evaluation[J]. China Brewing, 2016, 35(5): 16-19. doi: 10.11882/j.issn.0254-5071.2016.05.004
[17]	刘艳红, 季春艳, 程璐, 等. 模糊综合评定结合正交试验优化梨汁玫瑰饮料[J]. 保鲜与加工,2021,21(8):55−60. [LIU Y H, JI C Y, CHENG L, et al. Optimization of pear-rose beverage by fuzzy comprehensive evaluation combined with orthogonal experiment[J]. Storage and Process,2021,21(8):55−60. LIU Y H, JI C Y, CHENG L, et al. Optimization of pear-rose beverage by fuzzy comprehensive evaluation combined with orthogonal experiment[J]. Storage and Process, 2021, 21(8): 55-60.
[18]	任亚敏, 罗双群, 方爱丽, 等. 玛咖曲奇饼干生产配方优化及货架期预测[J]. 食品工业科技,2019,40(19):164−169. [REN Y M, LUO S Q, FANG A L, et al. Optimization of production formula and shelf life forecast of maca cookie[J]. Science and Technology of Food Industry,2019,40(19):164−169. REN Y M, LUO S Q, FANG A L, et al. Optimization of production formula and shelf life forecast of maca cookie[J]. Science and Technology of Food Industry, 2019, 40(19): 164-169.
[19]	席嘉佩, 詹萍, 田洪磊, 等. 基于感官模糊综合评价法与价值工程评价法的市售烤羊肉质量分析评价[J]. 食品科学,2019,40(7):60−67. [XI J P, ZHAN P, TIAN H L, et al. Quality analysis and evaluation of commercial roast mutton based on fuzzy comprehensive sensory evaluation and value engineering evaluation[J]. Science and Technology of Food Industry,2019,40(7):60−67. doi: 10.7506/spkx1002-6630-20180108-082 XI J P, ZHAN P, TIAN H L, et al. Quality analysis and evaluation of commercial roast mutton based on fuzzy comprehensive sensory evaluation and value engineering evaluation[J]. Science and Technology of Food Industry, 2019, 40(7): 60-67. doi: 10.7506/spkx1002-6630-20180108-082
[20]	刘艳红, 孙瑞雪, 刘玉洁, 等. 模糊感官评定结合响应面优化香椿苏打饼干工艺[J]. 包装与食品机械,2021,39(6):1−8. [LIU Y H, SUN R X, LIU Y J, et al. Optimization of the technology of toon soda biscuit by combination of fuzzy sensory evaluation and response surface[J]. Packaging and Food Machinery,2021,39(6):1−8. LIU Y H, SUN R X, LIU Y J, et al. Optimization of the technology of toon soda biscuit by combination of fuzzy sensory evaluation and response surface[J]. Packaging and Food Machinery, 2021, 39(6): 1-8.
[21]	程文龙, 王丽, 李雪, 等. 模糊数学感官评价法优化芦笋果糕加工工艺[J]. 食品研究与开发,2021,42(23):59−66. [CHENG W L, WANG L, LI X, et al. Optimization of processing technology of asparagus fruit cake by fuzzy mathematics sensory evaluation method[J]. Food Research and Development,2021,42(23):59−66. doi: 10.12161/j.issn.1005-6521.2021.23.010 CHENG W L, WANG L, LI X, et al. Optimization of processing technology of asparagus fruit cake by fuzzy mathematics sensory evaluation method[J]. Food Research and Development, 2021, 42(23): 59-66. doi: 10.12161/j.issn.1005-6521.2021.23.010
[22]	宋雁, 贾旭东, 崔文明, 等. 不同途径和剂量环磷酰胺建立小鼠免疫抑制模型的对比研究[J]. 中国食品卫生杂志,2013,25(3):218−225. [SONG Y, JIA X D, CUI W M, et al. Comparison research of immunosuppression models induced by different ways and doses of cyclophosphamide in mice[J]. Chinese Journal of Food Hygiene,2013,25(3):218−225. SONG Y, JIA X D, CUI W M, et al. Comparison research of immunosuppression models induced by different ways and doses of cyclophosphamide in mice[J]. Chinese Journal of Food Hygiene, 2013, 25(3): 218-225.
[23]	胡炀. 补肾固表颗粒对免疫低下小鼠的影响及安全性评价[D]. 晋中: 山西中医药大学, 2020. HU Y. Effect of Bushen Gubiao Granules on immunocompromised mice and its safety evaluation[D]. Jinzhong: Shanxi University of Traditional Chinese Medicine, 2020.
[24]	FENG S B, DING H Y, LIU L H, et al. Astragalus polysaccharide enhances the immune function of RAW264.7 macrophages via the NF-κB p65/MAPK signaling pathway[J]. Experimental and Therapeutic Medicine,2021,21(1):20−27.
[25]	LI W F, HU X Y, WANG S P, et al. Characterization and anti-tumor bioactivity of Astragalus polysaccharides by immunomodulation[J]. International Journal of Biological Macromolecules,2020,145:985−997. doi: 10.1016/j.ijbiomac.2019.09.189
[26]	GAO Z, ZHANG C, JING L, et al. The structural characterization and immune modulation activitives comparison of Codonopsis pilosula polysaccharide (CPPS) and selenizing CPPS (sCPPS) on mouse in vitro and vivo[J]. International Journal of Biological Macromolecules,2020,160:814−822. doi: 10.1016/j.ijbiomac.2020.05.149
[27]	钟金凤, 方热军. 环磷酰胺免疫抑制机制及在动物模型上的应用[J]. 中国免疫学杂志,2016,32(10):1541−1546. [ZHONG J F, FANG R J. Immunosuppressive mechanism of cyclophosphamide and its application in animal models[J]. Chinese Journal of Immunology,2016,32(10):1541−1546. doi: 10.3969/j.issn.1000-484X.2016.10.029 ZHONG J F, FANG R J. Immunosuppressive mechanism of cyclophosphamide and its application in animal models[J]. Chinese Journal of Immunology, 2016, 32(10): 1541-1546. doi: 10.3969/j.issn.1000-484X.2016.10.029
[28]	刘荣珍, 李凤文, 赵鹏, 等. 绿茶对小鼠通便作用的研究[J]. 实验动物科学与管理,2005,22(4):4−5,9. [LIU R Z, LI F W, ZHAO P, et al. Study of green tea on mice purgative function[J]. Laboratory Animal Science,2005,22(4):4−5,9. LIU R Z, LI F W, ZHAO P, et al. Study of green tea on mice purgative function[J]. Laboratory Animal Science, 2005, 22(4): 4-5, 9.
[29]	TSUDA M, HOSONO A, YANAGIBASHI T, et al. Prior stimulation of antigen-presenting cells with Lactobacillus regulates excessive antigen-specific cytokine responses in vitro when compared with bacteroides[J]. Cytotechnology,2007,55(2-3):89−101. doi: 10.1007/s10616-007-9104-1
[30]	陈远兴, 申茜, 王骥, 等. 红芪口服液增强免疫力的药理作用研究[J]. 天津中医药,2022,39(4):498−502. [CHEN Y X, SHEN Q, WANG J, et al. Pharmacological effect of Hongqi oral liquid on enhancing immunity[J]. Tianjin Journal of Traditional Chinese Medicine,2022,39(4):498−502. CHEN Y X, SHEN Q, WANG J, et al. Pharmacological effect of Hongqi oral liquid on enhancing immunity[J]. Tianjin Journal of Traditional Chinese Medicine, 2022, 39(4): 498-502.
[31]	塔来提·吐尔干, 张瑞青, 那布拉江·艾合买提, 等. 纳秒刀消融大鼠肝泡型包虫病灶对外周血免疫细胞的影响[J]. 中华肝胆外科杂志,2021,27(4):295−299. [TALAITI·T, ZHANG R Q, NABULAJIANG·A, et al. Experimental study on the peripheral blood immune cells NK, NKT and T cells induced by rat hepatic vesicular hydatid ablation with nanosecond knife at different energy levels[J]. Chinese Journal of Hepatobiliary Surgery,2021,27(4):295−299. doi: 10.3760/cma.j.cn113884-20200707-00358 TALAITI·Tuergan, ZHANG R Q, NABULAJIANG·Aihemaiti, et al. Experimental study on the peripheral blood immune cells NK, NKT and T cells induced by rat hepatic vesicular hydatid ablation with nanosecond knife at different energy levels[J]. Chinese Journal of Hepatobiliary Surgery, 2021, 27(4): 295-299. doi: 10.3760/cma.j.cn113884-20200707-00358
[32]	徐自慧, 王雅蕾, 万亮琴, 等. 肝癌小鼠外周血及脾脏T淋巴细胞亚群的变化及其意义[J]. 现代生物医学进展,2019,19(8):1406−1409. [XU Z H, WANG Y L, WAN L Q, et al. The changes of T cell subsets in peripheral blood and spleen and its significance in hepatoma mice[J]. Progress in Modern Biomedicine,2019,19(8):1406−1409. XU Z H, WANG Y L, WAN L Q, et al. The changes of T cell subsets in peripheral blood and spleen and its significance in hepatoma mice[J]. Progress in Modern Biomedicine, 2019, 19(8): 1406-1409.
[33]	TINAGO W, COGHLAN E, MACKEN A, et al. Clinical, immunological and treatment-related factors associated with normalised CD4⁺/CD8⁺ T-cell ratio: Effect of naive and memory T-cell subsets[J]. PLoS One,2014,9(5):e97011. doi: 10.1371/journal.pone.0097011
[34]	LIGHVANI A A, FRUCHT D M, JANKOVIC D, et al. T-bet is rapidly induced by interferon-gamma in lymphoid and myeloid cells[J]. Proceedings of the National Academy of Sciences of the United States of America,2001,98(26):15137−15142. doi: 10.1073/pnas.261570598
[35]	吕婧, 高燕, 李晨, 等. 基于斑马鱼模式生物的西洋参皂苷类成分增强免疫作用研究[J]. 中草药,2020,51(14):3728−3733. [LÜ J, GAO Y, LI C, et al. Effect of American ginseng saponins on enhancing immunity based on zebrafish model organisms[J]. Chinese Traditional and Herbal Drugs,2020,51(14):3728−3733. doi: 10.7501/j.issn.0253-2670.2020.14.016 LÜ J, GAO Y, LI C, et al. Effect of American ginseng saponins on enhancing immunity based on zebrafish model organisms[J]. Chinese Traditional and Herbal Drugs, 2020, 51(14): 3728-3733. doi: 10.7501/j.issn.0253-2670.2020.14.016
[36]	张旭杰, 贺卫超, 张伟杰. 芩连止泻汤对小儿慢性腹泻相关免疫功能的影响[J]. 世界中西医结合杂志,2020,15(4):691−693. [ZHANG X J, HE W C, ZHANG W J. Effect of Qinlian Zhixie Decoction on immune function related to chronic diarrhea in children[J]. World Journal of Integrated Traditional and Western Medicine,2020,15(4):691−693. ZHANG X J, HE W C, ZHANG W J. Effect of Qinlian Zhixie Decoction on immune function related to chronic diarrhea in children[J]. World Journal of Integrated Traditional and Western Medicine, 2020, 15(4): 691-693.

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