HE Xinyu, LI Zhihong, GAO Qian, et al. Study on the Enrichment Ability of Organic Calcium in Mycelium of Four Edible Fungi[J]. Science and Technology of Food Industry, 2023, 44(5): 82−87. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070104.
Citation: HE Xinyu, LI Zhihong, GAO Qian, et al. Study on the Enrichment Ability of Organic Calcium in Mycelium of Four Edible Fungi[J]. Science and Technology of Food Industry, 2023, 44(5): 82−87. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070104.

Study on the Enrichment Ability of Organic Calcium in Mycelium of Four Edible Fungi

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  • Received Date: July 12, 2022
  • Available Online: January 02, 2023
  • Objective: The edible fungi with high calcium enrichment rate and calcium organic degree were screened from sulfur fungus, Poria cocos, Monascus and Armillaria, and the effect of calcium enrichment on the content of the main active ingredient, dentate acid, was studied when the sulfur fungus with high calcium enrichment were cultured in liquid. Methods: The bioaccumulation method was used to study the effect of inorganic calcium on the growth of four edible fungi. The organic degree of calcium in mycelia was analyzed by static leaching method, and the content of the main active ingredient, dentate acid, was analyzed by HPLC. Results: In solid culture, the growth of sulfur fungus was better than that of Poria cocos, Armillaria and Monascus at the right amount of calcium addition. When the calcium addition amount was 100 mg/L in liquid culture, the calcium enrichment rate of sulfur fungus was significantly higher than those of Poria cocos, Armillaria and Monascus. When the calcium concentration of sulfur fungus liquid culture was 100 mg/L, the calcium enrichment rate was 93.51%, the degree of calcium organization was 85.85%, and the content of the dentate acid was 18.34 mg/g. Therefore, sulfur fungus has the potential to become a new functional calcium supplement product.
  • [1]
    顾清. 钙的生理功能及代谢的研究进展(综述)[J]. 中国食品卫生杂志,2002,14(6):29−33. [GU Qing. Research progress of physiological function and metabolism of calcium (Review)[J]. Chinese Journal of Food Hygiene,2002,14(6):29−33. doi: 10.3969/j.issn.1004-8456.2002.06.012
    [2]
    张爱民, 薛建平. 膳食营养要素钙的代谢及生理功能研究进展[J]. 淮北煤师院学报(自然科学版),2001,22(4):49−54. [ZHANG Aimin, XUE Jianping. Research progress in metabolism and physiological function of dietary nutrient element calcium[J]. Journal of Huaibei Coal Teachers College (Natural Science Edition),2001,22(4):49−54.
    [3]
    潘恩霖. 钙与人体健康[J]. 福建轻纺,2002(7):22−26. [PAN Enlin. Calcium and human health[J]. Fujian Light Textile,2002(7):22−26. doi: 10.3969/j.issn.1007-550X.2002.07.007
    [4]
    HERRERARODRIGUEZ S E, GARCIAMARQUEZ E, PADILLACAMBEROS E, et al. Evaluation of an ionic calcium fiber supplement and its impact on bone health preservation in a dietary calcium deficiency mice model[J]. Nutrients,2022,14(3):422−422. doi: 10.3390/nu14030422
    [5]
    李海潮, 韦开卫, 王斌, 等. 原子吸收法测定31种蔬菜水果中的微量元素含量[J]. 国外医学(医学地理分册),2013,34(3):187−190. [LI Haichao, WEI Kaiwei, WANG Bin, et al. Determination of trace elements in 31 kinds of vegetables and fruits by atomic absorption spectrometry[J]. Foreign Medical Sciences (Medical Geography),2013,34(3):187−190. doi: 10.3969/j.issn.1001-8883.2013.03.014
    [6]
    MAYS S, BRICKLEY M. Is dietary deficiency of calcium a factor in rickets? Use of current evidence for our understanding of the disease in the past[J]. International Journal of Paleopathology,2022,36:36−44. doi: 10.1016/j.ijpp.2021.11.001
    [7]
    BOUILLON R. Nutritional rickets: Calcium or vitamin D deficiency?[J]. The American Journal of Clinical Nutrition,2021,114(1):3−4. doi: 10.1093/ajcn/nqab121
    [8]
    鲍建民. 钙的生理功能及吸收利用[J]. 微量元素与健康研究,2006(4):65−66. [BAO Jianmin. Physiological function and absorption and utilization of calcium[J]. Trace Elements and Health Research,2006(4):65−66. doi: 10.3969/j.issn.1005-5320.2006.04.028
    [9]
    高爱民. 人体必需的营养元素——钙[J]. 微量元素与健康研究,2005,22(1):66−66. [GAO Aimin. Essential nutrient element of human body-calcium[J]. Trace Elements and Health Research,2005,22(1):66−66. doi: 10.3969/j.issn.1005-5320.2005.01.032
    [10]
    MEGAN R B, ALEXANDER E T. Intestinal absorption and renal reabsorption of calcium throughout postnatal development[J]. Experimental Biology and Medicine,2017,242(8):840−849. doi: 10.1177/1535370217699536
    [11]
    SUNMIN P, SUNA K, KIM D S. Severe calcium deficiency increased visceral fat accumulation, down-regulating genes associated with fat oxidation, and increased insulin resistance while elevating serum parathyroid hormone in estrogen-deficient rats[J]. Nutrition Research,2020,73(C):48−57.
    [12]
    卜庆梅, 王丽娟, 刘林德, 等. 3种食用菌菌丝体富钙的研究[J]. 食品科学,2009,30(21):172−174. [BU Qingmei, WANG Lijuan, LIU Linde, et al. Study on calcium enrichment in mycelium of three edible fungi[J]. Food Science,2009,30(21):172−174. doi: 10.3321/j.issn:1002-6630.2009.21.040
    [13]
    李慧英. 蛹虫草富铁液体深层发酵条件的优化[J]. 食品研究与开发,2017,38(7):153−159. [LI Huiying. Optimization of fermentation conditions of iron-rich Cordyceps militaris[J]. Food Research and Development,2017,38(7):153−159. doi: 10.3969/j.issn.1005-6521.2017.07.034
    [14]
    姜宁, 朱淑彤, 苏浩雨, 等. 猴头菌菌丝富硒特性及富硒深层发酵的研究[J]. 天然产物研究与开发,2021,33(5):810−818, 830. [JIANG Ning, ZHU Shutong, SU Haoyu, et al. Study on selenium-rich characteristics of Hericiumhericium mycelia and its deep fermentation[J]. Natural Product Research and Development,2021,33(5):810−818, 830. doi: 10.16333/j.1001-6880.2021.5.013
    [15]
    SABA E, SONG Y M, JEON B R, et al. Acetyl eburicoic acid from Laetiporus sulphureus var. miniatus suppresses inflammation in murine macrophage RAW 264.7 cells[J]. Mycobiology,2015,43(2):131−136. doi: 10.5941/MYCO.2015.43.2.131
    [16]
    王娟. 硫磺菌和灰盖鬼伞发酵产物中活性成分的初步研究[D]. 杨凌: 西北农林科技大学, 2007

    WANG Juan. Preliminary study on the active constituents in the fermentation products of Sulfurobacillus vulgaris and Ambrosia chinensis[D]. Yangling: Northwest A & F University, 2007.
    [17]
    闫梅霞, 关一鸣, 胡清秀, 等. 硫磺菌菌丝体粗多糖对小鼠Lewis肺癌的体内抑制[J]. 食用菌学报,2011,18(1):46−48. [YAN Meixia, GUAN Yiming, HU Qingxiu, et al. Antitumor activity of crude polysaccharide from Laetiporus sulphureus[J]. Journal of Edible Fungi,2011,18(1):46−48. doi: 10.3969/j.issn.1005-9873.2011.01.007
    [18]
    李慧君, 王天合, 尤朋涛, 等. 不同产地茯苓对肾阳虚下焦水肿大鼠的利水渗湿作用研究[J]. 中药新药与临床药理,2021,32(5):632−638. [LI Huijun, WANG Tianhe, YOU Pengtao, et al. Effects of Poria cocos from different habitats on the hydration and osmosis in rats with focal edema under kidney Yang deficiency[J]. Chinese Journal of New Drugs and Clinical Pharmacology,2021,32(5):632−638. doi: 10.19378/j.issn.1003-9783.2021.05.006
    [19]
    王悦, 田双双, 刘晓谦, 等. 茯苓多糖的提取、结构及药理作用研究进展[J]. 世界中医药,2021,16(17):2548−2555. [WANG Yue, TIAN Shuangshuang, LIU Xiaoqian, et al. Research progress on extraction, structure and pharmacological effects of polysaccharides from Poria cocos[J]. World Journal of Chinese Medicine,2021,16(17):2548−2555. doi: 10.3969/j.issn.1673-7202.2021.17.006
    [20]
    马美荣, 张坤, 李洪媛, 等. 麸皮红曲发酵工艺研究[J]. 中国调味品,2022,47(7):167−170, 181. [MA Meirong, ZHANG Kun, LI Hongyuan, et al. Study on fermentation technology of wheat bran Monascus[J]. Chinese Condiments,2022,47(7):167−170, 181. doi: 10.3969/j.issn.1000-9973.2022.07.031
    [21]
    胡德, 李正龙, 赵威, 等. 蜜环菌菌丝与菌索活性成分含量研究[J]. 食品安全质量检测学报,2022,13(6):1748−1754. [HU De, LI Zhenglong, ZHAO Wei, et al. Study on the content of active ingredients in mycelia and mycordia of Armillaria[J]. Chinese Journal of Food Safety and Quality,2022,13(6):1748−1754. doi: 10.3969/j.issn.2095-0381.2022.6.spaqzljcjs202206007
    [22]
    李金平, 何美静, 李梦雪,等. 浅谈硫磺多孔菌的研究[J]. 生物化工,2019,5(6):129−130, 134. [LI Jinping, HE Meijing, LI Mengxue, et al. Discussion on the research progress of sulfur porous bacteria[J]. Biochemical Engineering,2019,5(6):129−130, 134. doi: 10.3969/j.issn.2096-0387.2019.06.038
    [23]
    张琳, 汪鋆植, 余海立,等. 齿孔酸对高尿酸血症黄嘌呤氧化酶活性的影响[J]. 生物资源,2017,39(2):102−107. [ZHANG Lin, WANG Yunzhi, YU Haili, et al. Effects of eburicoic acid on xanthine oxidase activity of hyperuricemia[J]. Bioresources,2017,39(2):102−107.
    [24]
    HUANG G J, DENG J S, HUANG S S, et al. Hepatoprotective effects of eburicoic acid and dehydroeburicoic acid from Antrodia camphorata in a mouse model of acute hepatic injury[J]. Food Chemistry,2013,141(3):3020−3027. doi: 10.1016/j.foodchem.2013.03.061
    [25]
    SUN X W, LI Q G. The main components analysis of Laetiporus sulphureu crude extract and its hepatoprotective effect on carbon tetrachloride-induced hepatic fibrosis in rats[J]. Applied Mechanics and Materials,2014,3252(568-570):1934−1939.
    [26]
    WANG J Z, ZHANG P, HE H B, et al. Eburicoic acid from Laetiporus sulphureus (Bull.: Fr.) Murrill attenuates inflammatory responses through inhibiting LPS-induced activation of PI3K/Akt/mTOR/NF-κB pathways in RAW264.7 cells[J]. Naunyn-Schmiedeberg's Archives of Pharmacology,2017,390(8):845−856. doi: 10.1007/s00210-017-1382-3
    [27]
    李红霞, 李国江, 张俊杰,等. EDTA络合滴定钙镁指示剂的改进[J]. 河北理工学院学报,2003(4):142−150. [LI Hongxia, LI Guojiang, ZHANG Junjie, et al. Improvement of indicators in titration analysis of calcium and magnesium with EDTA[J]. Journal of Hebei Institute of Technology,2003(4):142−150.
    [28]
    牛江梅, 沈乐琴, 孙翔. 湿法消化方法测定食品中铜、锌[J]. 中国公共卫生,2003(5):109. [NIU Jiangmei, SHEN Leqin, SUN Xiang. Determination of copper and zinc in food by wet digestion method[J]. Chinese Public Health,2003(5):109. doi: 10.11847/zgggws2003-19-05-69
    [29]
    张新国, 马国弟, 刘子预, 等. 有机钙转化微生物的筛选、鉴定及其富钙研究[J]. 中国食品学报,2017,17(9):251−259. [ZHANG Xinguo, MA Guodi, LIU Ziyu, et al. Screening and identification of organic calcium enriched microorganism from natural environment and it's characteristic[J]. Chinese Journal of Food Science and Technology,2017,17(9):251−259. doi: 10.16429/j.1009-7848.2017.09.032
    [30]
    秦义. 富锌灵芝液态发酵研究[D]. 杨凌: 西北农林科技大学, 2007

    QIN Yi. Liquid fermentation of zinc-rich Ganoderma lucidum[D]. Yangling: Northwest A & F University, 2007.
    [31]
    孙希雯, 李奇庚. 金针菇富锌条件及锌结合形态的研究[J]. 微生物学报,1997(1):40−46. [SUN Xiwen, LI Qigeng. Study on zinc enrichment conditions and Zn-binding morphology of Flammulinavelina[J]. Acta MicrobiologicaSinica,1997(1):40−46. doi: 10.13343/j.cnki.wsxb.1997.01.007
    [32]
    孙文军, 汪鋆植, 张盼, 等. 硫磺菌液体培养条件的优化研究[J]. 三峡大学学报(自然科学版),2015,37(3):100−103. [SUN Wenjun, WANG Junzhi, ZHANG Pan, et al. Study of optimum culture medium of Laetiporus sulphureus liquid fermentation[J]. Journal of China Three Gorges University (Natural Science Edition),2015,37(3):100−103.
    [33]
    王伟霞, 李福后, 陈立国. 茯苓菌丝体富集钙的研究[J]. 食用菌,2007(1):9−10. [WANG Weixia, LI Fuhou, CHEN Liguo. Study on calcium enrichment in mycelia of Poria cocos[J]. Edible Fungi,2007(1):9−10. doi: 10.3969/j.issn.1000-8357.2007.01.005
    [34]
    许玉灿. 有机锌的富集载体及检测方法综述[J]. 生物技术世界,2016(3):15−16. [XU Yucan. Review on enrichment carriers and detection methods of organic zinc[J]. Biotechnology World,2016(3):15−16.
    [35]
    张婷, 钱亮亮, 王伟霞, 等. 斑玉蕈菌丝体富集钙的研究[J]. 北方园艺,2022(4):104−109. [ZHANG Ting, QIAN Liangliang, WANG Weixia, et al. Study on calcium enrichment in the mycelium of carygoides[J]. Northern Horticulture,2022(4):104−109.

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