Anti-aging Effects and Mechanisms of Recombinant Human-derived Collagen on Aging Mouse Induced by D-Galactose

LI Jia; AN Miaoqing; LÜ Chenhao; GUO Rongxiang; DU Bing; HE Qing

doi:10.13386/j.issn1002-0306.2022070013

Volume 44 Issue 10

May 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(10): 343-352. > DOI: 10.13386/j.issn1002-0306.2022070013

LI Jia, AN Miaoqing, LÜ Chenhao, et al. Anti-aging Effects and Mechanisms of Recombinant Human-derived Collagen on Aging Mouse Induced by D-Galactose[J]. Science and Technology of Food Industry, 2023, 44(10): 343−352. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070013.

Citation:

PDF (2856 KB)

Anti-aging Effects and Mechanisms of Recombinant Human-derived Collagen on Aging Mouse Induced by D-Galactose

1.
Jiangsu Aland Nutrition Co., Ltd., Taizhou 214500, China
2.
College of Food Science, South China Agricultural University, Guangzhou 510000, China

More Information

Received Date: July 03, 2022
Available Online: March 20, 2023

Graphical Abstract

Abstract

Abstract

In order to explore the anti-aging effect and mechanism of recombinant recombinant human-derived collagen (RHC), the aging mouse model was constructed through 300 mg/kg D-galactose intraperitoneal injection, and different doses of RHC were used to study the changes of hyaluronic acid, collagen, lipofuscin, hydroxyproline content, antioxidant enzyme activity, key genes and protein expression related to epidermal cell proliferation and differentiation and collagen synthesis. The results showed that compared with the aging model group (SLM), the intervention groups that was gavaged RHC had significantly less skin folds, higher collagen content (P<0.05) and superoxide dismutase (SOD), while lower malondialdehyde (MDA) content in the skins (P<0.0001). Compared with the SLM group, antioxidant indicators such as glutathione peroxidase (GSH-Px) content were increased in the RHC intervention groups, while lipofuscin content and pathological scores were reduced, and significant effect were also observed. In addition, the expression levels of key genes and protein (EGFR, TIMP-1, COL1A2, COL3A1, TGF-βⅠ, TGF-βⅡ) were increased, while those of MMP-1, MMP-3 in the skin of mouse were decreased in the RHC intervention groups. In conclusion, RHC has a good anti-aging effect on the skin, and its mechanism may be related to increase antioxidant effect, promote the EGFR, TGF pathway, and modulate the expression balance of MMPs and TIMPs, promote the proliferation and differentiation of epidermal cells, and the synthesis of collagen.
- recombinant human-derived collagen,
- D-galactose,
- aging model,
- anti-aging,
- lipofuscin

FullText(HTML)

References (45)

References

[1]	TURNHEIM K. When drug therapy gets old: Pharmacokinetics and pharmacodynamics in the elderly[J]. Experimental Gerontology,2003,38(8):843−853. doi: 10.1016/S0531-5565(03)00133-5
[2]	魏姝玥, 王海英, 李爱兰, 等. 脂肪干细胞对D-半乳糖致裸鼠皮肤老化的拮抗作用及对皮肤恢复功能的影响[J]. 中国美容医学,2020,29(1):62−67. [WEI Z Y, WANG H Y, LI A L, et al. Antagonistic effect of adipose-derived stem cells on skin aging induced by D-galactose in nude mice and its effect on skin recovery[J]. Chinese Journal of Aesthetic Medicine,2020,29(1):62−67. doi: 10.15909/j.cnki.cn61-1347/r.003476
[3]	CHATTOPADHYAY S, RAINES R T. Review collagen-based biomaterials for wound healing[J]. Biopolymers,2014,101(8):21−33.
[4]	蔡东联. 不可忽视老年斑的危害[J]. 求医问药,2012(10):41. [CAI D L. Don't ignore the dangers of age spots[J]. Seek Medical,2012(10):41.
[5]	OGRODNIK M, SALMONOWICZ H, GLADYSHEV V N. Integrating cellular senescence with the concept of damage accumulation in aging: Relevance for clearance of senescent cells[J]. Aging Cell,2019,18(1):e12841. doi: 10.1111/acel.12841
[6]	KUDRYAVTSEVA A V, KRASNOV G S, DMITRIEV A A, et al. Mitochondrial dysfunction and oxidative stress in aging and cancer[J]. Oncotarget,2016,7(29):44879−44905. doi: 10.18632/oncotarget.9821
[7]	TERMAN A, KURZ T, NAVRATIL M, et al. Mitochondrial turnover and aging of long-lived postmitotic cells: The mitochondrial-lysosomal axis theory of aging[J]. Antioxidants & Redox Signaling,2010,12(4):503−535.
[8]	SARETZKI G. Telomeres, telomerase and ageing[J]. Sub-Cellular Biochemistry,2018,9:221−308.
[9]	DAVALLI P, MITIC T, CAPORALI A, et al. ROS, cell senescence, and novel molecular mechanisms in aging and age-related diseases[J]. Oxidative Medicine and Cellular Longevity,2016,2016:3565127.
[10]	AUNG-HTUT M T, AYER A, BREITENBACH M, et al. Oxidative stresses and ageing[J]. Subcell Biochem,2012,57:13−54.
[11]	GOMEZ-LINTON D R, ALAVEZ S, ALARCON-AGUILA A, et al. Some naturally occurring compounds that increase longevity and stress resistance in model organisms of aging[J]. Biogerontology,2019,20(5):583−603. doi: 10.1007/s10522-019-09817-2
[12]	米钰, 惠俊峰, 范代娣, 等. 类人胶原蛋白生物相容性实验研究[J]. 西北大学学报( 自然科学版),2004,34(1):66−72. [MI Y, HUI J F, FAN D D, et al. The biocompatibility of human-like collagen[J]. Journal of Northwest University (Natural Science Edition),2004,34(1):66−72.
[13]	YOON D, YOON D, CHA H J, et al. Enhancement of wound healing efficiency mediated by artificial dermis functionalized with EGF or NRG1[J]. Biomedical Materials (Bristol, England),2018,13(4):45007. doi: 10.1088/1748-605X/aaac37
[14]	余建军, 赵铧. 重组类人胶原蛋白研究应用进展[J]. 日用化学品科学,2022,45(2):48−51. [YU J J, ZHAO H. Progress in the research and application of recombinant human collagen[J]. Daily Chemicals Science,2022,45(2):48−51. doi: 10.3969/j.issn.1006-7264.2022.02.011
[15]	YUAN T, ZHANG L, LI K F, et al. Collagen hydrogel as an immunomodulatory scaffold in cartilage tissue engineering[J]. Journal of Biomedical Materials Research. Part B, Applied Biomaterials,2014,102(2):337−344. doi: 10.1002/jbm.b.33011
[16]	AN B, LIN Y S, BRODSKY B, et al. Collagen interactions: Drug design and delivery[J]. Advanced Drug Delivery Reviews,2016,97:69−84. doi: 10.1016/j.addr.2015.11.013
[17]	JIA Q, LUO Y. The selective roles of chaperone systems on overexpression of human-like collagen in recombinant Escherichia coli[J]. J Ind Microbiol Biotechnol,2014,41(11):1667−1675. doi: 10.1007/s10295-014-1500-x
[18]	TANG Y, YANG X, HANG B, et al. Efficient production of hydroxylated human-like collagen via the co-expression of three key genes in Escherichia coli origami (DE3)[J]. Applied Biochemistry and Biotechnology,2016,178(7):1458−1470. doi: 10.1007/s12010-015-1959-6
[19]	齐志峰, 郑双进, 张晓瑜, 等. 射频联合重组人源胶原蛋白凝胶修复面部皮肤屏障功能的研究[J]. 中国医疗美容,2020,10(3):48−52. [QI Z F, ZHENG S J, ZHANG X Y, et al. Radiofrequency combined with recombinant human collagen-gel to repair the function of facial skin barrier[J]. China Medical Cosmetology,2020,10(3):48−52. doi: 10.19593/j.issn.2095-0721.2020.03.013
[20]	郭亚媛. 重组人胶原蛋白水凝胶复合成纤维细胞修复皮肤缺损的实验研究[D]. 西安: 西北大学, 2019. GUO Y Y. The study of recombinant human collagen hydrogel containing fibroblastsin repairing skin defect[D]. Xi'an: Northwest University, 2019.
[21]	牛佳玮, 莫玲, 王子锃, 等. 恭城油茶减轻D-半乳糖致小鼠衰老的氧化应激[J]. 现代食品科技,2021,37(4):1−6. [NIU J W, MO L, WANG Z C, et al. Gongcheng oil tea attenuates oxidative stress in d-galactose-induced aging mice[J]. Modern Food Science & Technology,2021,37(4):1−6. doi: 10.13982/j.mfst.1673-9078.2021.4.0913
[22]	XIAO LIAN F, XIAO FANG Z, SHI QI L, et al. Preparation of aminated fish scale collagen and oxidized sodium alginate hybrid hydrogel for enhanced full-thickness wound healing[J]. International Journal of Biological Macromolecules,2020,164:626−637. doi: 10.1016/j.ijbiomac.2020.07.058
[23]	JING C, PAN W, YAN NAN L, et al. Double crosslinked HLC-CCS hydrogel tissue engineering scaffold for skin wound healing[J]. International Journal of Biological Macromolecules,2020,155:625−635. doi: 10.1016/j.ijbiomac.2020.03.236
[24]	LEI H, CHEN HUI ZHU, DAI DI F. Optimization of human-like collagen composite polysaccharide hydrogel dressing preparation using response surface for burn repair[J]. Carbohydrate Polymers, 2020, 239.
[25]	HUI Z, YU XIAO L, CAN WEN C, et al. Responsive drug-delivery microcarriers based on the silk fibroin inverse opal scaffolds for controllable drug release[J]. Applied Materials Today, 2020, 19(C): 100540.
[26]	FAROKHI M, MOTTAGHITALAB F, REIS R L, et al. Functionalized silk fibroin nanofibers as drug carriers: Advantages and challenges[J]. Journal of Controlled Release, 2020, 321(C): 324-347.
[27]	宋晓娟. 类人胶原蛋白治疗颜面再发性皮炎疗效观察及研究[J]. 吉林医学,2016,37(9):2259−2260. [SONG X J. Study on the efficacy of human-like collagen in the treatment of facial reoccurring dermatitis[J]. Jilin Medical Journal,2016,37(9):2259−2260. doi: 10.3969/j.issn.1004-0412.2016.09.088
[28]	KONG S Z, LI JC, LI S D, et al. Anti-aging effect of chitosan oligosaccharide on d-galactose-induced subacute aging in mice[J]. Mar Drugs,2018,16(6):181. doi: 10.3390/md16060181
[29]	薛文, 秦菊, 努尔买买提·阿穆提, 等. 不同ELISA和AGID试剂盒检测马传染性贫血病毒抗体的比较[J]. 中国动物检疫,2022,39(7):103−107. [XUE W, QIN J, NURMAIMAITI·A, et al. Comparison of different ELISA and AGID kits for equine infectious anemia virus antibodies[J]. Chinese Animal Quarantine,2022,39(7):103−107.
[30]	钟召兵. 澳州进口与中国本地荷斯坦牛血清抗氧化指标和微量元素含量比较研究[J]. 中国奶牛,2022(7):16−19. [ZHONG Z B. Comparative study on antioxidant index and trace element content of Australian imported and local Holstein cattle in China[J]. Chinese Dairy Cows,2022(7):16−19. doi: 10.19305/j.cnki.11-3009/s.2022.07.004
[31]	林才云, 王联珠, 许加超, 等. 培养条件对副溶血性弧菌AI-2活性及其合成关键基因表达水平的影响[J]. 食品安全质量检测学报,2020,11(9):2924−2929. [LIN C Y, WANG L Z, XU J C, et al. Effects of culture conditions on AI-2 activity of V. parahaemolyticus and the expression levels of key genes for synthesis[J]. Food Safety and Quality Testing Journal,2020,11(9):2924−2929.
[32]	康俞莉, 赵颖, 章强强. 荧光原位杂交法观察小鼠皮肤组织切片中型无绿藻感染的研究[J]. 中华皮肤科杂志,2014,47(9):646−649. [KANG Y L, ZHAO Y, ZHANG Q Q. Observation of medium-free green algae infection in mouse skin tissue sections by fluorescence in situ hybridization[J]. The Chinese Journal of Dermatology,2014,47(9):646−649.
[33]	熊慧, 戢丹菊, 杨家林, 等. 九龙虫对衰老小鼠皮肤的影响[J]. 鄂州大学学报,2021,28(5):108−110. [XIONG H, YANG J L, LIU D H, et al. Effects of Kowloon worm on skin of aged mice[J]. The Journal of Ezhou University,2021,28(5):108−110. doi: 10.16732/j.cnki.jeu.2021.05.037
[34]	颜泽清, 娄红军, 王元. 透明质酸对犬皮肤水分的改善效果研究[J]. 中国工作犬业,2019(9):14−15. [YAN Z Q, LOU H J, WANG Y. Study on the effect of hyaluronic acid on the improvement of skin moisture in dogs[J]. China Working Dog,2019(9):14−15.
[35]	TOMOKO, ASAI T, OIKAWA F, et al. Food-derived collagen peptides, Prolyl-Hydroxyproline (Pro-Hyp), and Hydroxyprolyl-Glycine (Hyp-Gly) enhance growth of primary cultured mouse skin fibroblast using fetal bovine serum free from hydroxyprolyl peptide[J]. International Journal of Molecular Sciences,2019,21(1):229.
[36]	GILCHREST B A, GARMYS M, YEAR M. Aging and photoaging effect gene expression in cultured human keratinocyte[J]. Arch Dermatol,1994,130(1):82−86. doi: 10.1001/archderm.1994.01690010086013
[37]	李卫, 元发芝. 皮肤衰老及抗衰老研究进展[J]. 中华医学美学美容杂志,2004,10(1):60−61. [LI W, YUAN F Z. Advances in skin ageing and anti-ageing research[J]. Chinese Journal of Medical Aesthetics and Cosmetology,2004,10(1):60−61.
[38]	伍春. 党参多糖对D-半乳糖致衰老小鼠皮肤抗氧化作用及基因表达谱的影响[D]. 兰州: 甘肃中医学院, 2014 WU C. Effect of Dangshen polysaccharide on skin antioxidant effect and gene expression profile of D-galactose-induced aging mice[D]. Lanzhou: College of Traditional Chinese Medicine, 2014.
[39]	王振富. 党参对大强度训练大鼠心肌线粒体抗氧化能力的的影响[J]. 江苏医药,2011,37(13):1515−1518. [WANG Z F. Effect of Dangshen on the antioxidant capacity of myocardial mitochondria in intensively trained rats[J]. Jiangsu Medicine,2011,37(13):1515−1518.
[40]	王岚, 张帆, 王艳, 等. 甘肃党参水提物对 D-半乳糖所致衰老小鼠脑组织 SOD、MDA 影响的实验研究[J]. 中国中医药科技,2010,17(2):148−149. [WANG L, ZHANG F, WANG Y, et al. Experimental study on the effect of Gansu Dangshen water extract on brain tissue SOD and MDA in aging mice caused by D-galactose[J]. Chinese TCM Science and Technology,2010,17(2):148−149.
[41]	WILSON J, BALKWILL F. The role of cytokines in the epithelial cancer microenvironment[J]. Seminars in Cancer Biology,2002,12(2):113−120.
[42]	SHEN B C, LIEN H C, H N, et al. Increased expression and activation of gelatinolytic matrix metalloproteinases is associated with the progression and recurrence of human cervical cancer[J]. Cancer Research,2003,63(10):6537−6542.
[43]	NIETO N. 335 Kupffer cells induce a fibrogenic response by transactivating the COL1A1 and COL1A2 promoters and by decreasing collagen I degradation[J]. Journal of Hepatology,2006,44(S2):S129−S129.
[44]	QUAN T, HE T, KANG S, et al. Ultraviolet Irradiation alters transforming growth factor β/smad pathway in human skin in vivo[J]. Journal of Investigative Dermatology,2002,119(2):499−506. doi: 10.1046/j.1523-1747.2002.01834.x
[45]	马作霖. 乳清粉调节D-半乳糖诱导衰老小鼠肠道菌群结构及抗衰老作用研究[D]. 兰州: 甘肃农业大学, 2021. MA Z L. Study on intestinal flora structure and anti-aging effect of D-galactose [D]. Lanzhou: Gansu Agricultural University, 2021.

[1]	WANG Wei, ZHANG Xu, ZHANG Jia-min, JI Li-li, KANG Jun, BAI Ting. The Processing and Storage Characteristics of Sichuan Sauce-flavored Air-dried Sausage and Its Characteristics of “Shallow Fermentation”[J]. Science and Technology of Food Industry, 2021, 42(1): 82-88. DOI: 10.13386/j.issn1002-0306.2020030005
[2]	LIU Na, PAN Xing-lu, ZHANG Shuang, YANG Qing-xi, JI Ming-shan, ZHANG Zhi-hong. Effect of Storage and Preservation and Processing on the Flutriafol Residues in Strawberry and Processed Products[J]. Science and Technology of Food Industry, 2018, 39(14): 219-222. DOI: 10.13386/j.issn1002-0306.2018.14.041
[3]	LIU Zhi-dong, CHEN Xue-zhong, HUANG Hong-liang, WANG Yi-hong, LIU Qin, CHEN Yong, QU Ying-hong, QU Tai-chun, SONG Xue-feng. Progress in processing and storaging technologies of Antarctic krill (Euphausia superba) powder[J]. Science and Technology of Food Industry, 2016, (16): 357-361. DOI: 10.13386/j.issn1002-0306.2016.16.063
[4]	DONG Yi-wei, GUO Quan-you, LI Bao-guo, JIANG Chao-jun, GU Tian-sheng. Identification of dominated spoilage organisms and quality changes in lightly salted Mylopharyngodon piceus during processing and storage[J]. Science and Technology of Food Industry, 2015, (23): 306-310. DOI: 10.13386/j.issn1002-0306.2015.23.055
[5]	XU Rong-xiong, DENG Rui-jun. Study on the processing of diced pineapple jam[J]. Science and Technology of Food Industry, 2015, (23): 252-255. DOI: 10.13386/j.issn1002-0306.2015.23.043
[6]	CHEN Xue-hong, QIN Wei-dong, MA Li-hua, DAI Xiao-juan. Effect of processing conditions on quality of fruit and vegetable juices[J]. Science and Technology of Food Industry, 2014, (01): 355-362. DOI: 10.13386/j.issn1002-0306.2014.01.046
[7]	YE Bin-qing, TAO Ning-ping, WANG Xi-chang, ZHU Qing-cheng. Research progress in nutritional evaluation, storage and processing of Cololabis saira and comprehensive utilization of its processing by-products[J]. Science and Technology of Food Industry, 2013, (22): 367-370. DOI: 10.13386/j.issn1002-0306.2013.22.013
[8]	蓝浆果原汁及果汁饮料加工技术[J]. Science and Technology of Food Industry, 1999, (05): 38-40. DOI: 10.13386/j.issn1002-0306.1999.05.013
[9]	发酵法桑汁饮料加工工艺的研究[J]. Science and Technology of Food Industry, 1999, (05): 35-37. DOI: 10.13386/j.issn1002-0306.1999.05.012
[10]	绿色蔬菜在贮存、加工过程中绿色损失的机制、途径及其控制[J]. Science and Technology of Food Industry, 1999, (05): 19-21. DOI: 10.13386/j.issn1002-0306.1999.05.005

Supplements (0)

Cited By

Cited by

Periodical cited type(8)

1.	王佳，丁方莉，安宇，曾雪莹，张智慧，李思楠，徐开媛，周芳，王颖，张璐，徐炳政，孙泽堃. 芸豆-蓝靛果复合发酵液制备工艺优化及其抗氧化活性. 食品工业科技. 2025(03): 222-231 . 本站查看
2.	王虎玄，柯西娜，王聪，朱亚南，孙宏民. 苹果酵素的制备及其抗氧化功能研究. 陕西科技大学学报. 2023(03): 37-46 .
3.	杨彬彦，党娅，黎坤怡. 蓝莓酵素复合菌种发酵工艺优化及品质分析. 中国酿造. 2023(12): 165-169 .
4.	陈洲琴，张祝兰，程贤，林仙菊，杨煌建，严雪浪，朱爱明，连云阳. 枇杷酵素发酵过程生物学特性和主要功效酶活性研究. 福建农业科技. 2023(10): 23-28 .
5.	秦宇蒙，王艳丽，周笑犁，董平坤，吴栋斐. 番茄酵素自然发酵过程中主要功效酶的变化. 食品工业科技. 2022(20): 60-66 . 本站查看
6.	蒋家璇，韩盼盼，孔振杨，程陆陆，姚沛琳. 百香果酵素自然发酵过程中代谢产物及抗氧化活性研究. 农产品加工. 2022(19): 10-13+17 .
7.	任秀秀，余冬丽，郭涛，郭正江，LUO Liu. 餐余酵素中益生菌的分离培养及鉴定. 贵州工程应用技术学院学报. 2021(03): 61-67 .
8.	张焱梅，甘玉芬，丁学梅，马海燕，伍凤莲，冯玉兰. 植物酵素的活性功效及其食用方面的研究进展. 甘肃科技纵横. 2021(08): 25-28 .