Uncertainty Evaluation for Determination of 6 Sildenafil Derivatives in Oral Liquid by UPLC-Q/Orbitrap HRMS

ZHANG Shenping; HOU Chao; ZHANG Ni; DU Ruyun; ZHOU Jing; LIU Yang

doi:10.13386/j.issn1002-0306.2022060278

Volume 44 Issue 11

May 2023

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Science and Technology of Food Industry > 2023 > 44(11): 280-286. > DOI: 10.13386/j.issn1002-0306.2022060278

ZHANG Shenping, HOU Chao, ZHANG Ni, et al. Uncertainty Evaluation for Determination of 6 Sildenafil Derivatives in Oral Liquid by UPLC-Q/Orbitrap HRMS[J]. Science and Technology of Food Industry, 2023, 44(11): 280−286. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022060278.

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Uncertainty Evaluation for Determination of 6 Sildenafil Derivatives in Oral Liquid by UPLC-Q/Orbitrap HRMS

Shanghai Institute of Quality Inspection and Technical Research, Key Laboratory of Milk and Dairy Products Detection and Monitoring Technology for State Market Regulation, Shanghai 200233, China

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Received Date: June 27, 2022
Available Online: March 29, 2023

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Abstract

Abstract

In order to ensure the accuracy and reliability of high resolution mass spectrometry for the determination of sildenafil derivatives in aphrodisiac oral liquid, it is necessary to evaluate the uncertainty of the determination results. In this study, the content of 6 sildenafil derivatives in oral liquid health food was determined by ultra-high performance liquid chromatography with quadrupole/electrostatic field Orbitrap high resolution mass spectrometry (UPLC-Q/Orbitrap HRMS). The mathematical model was established and the measurement uncertainty was evaluated according to JJF 1059.1-2012 “Evaluation and Expression of Measurement Uncertainty”. When the contents of N-demethyl sildenafil, dimethyl thiosildenafil, dimethyl sildenafil, hydroxythiocarmo sildenafil, thiocarmo sildenafil and thiosildenafil in oral solution were 0.90, 1.04, 1.22, 2.01, 1.51 and 1.14 mg/kg, the extended uncertainties were 0.07, 0.09, 0.10, 0.16, 0.13 and 0.09 mg/kg (k=2), respectively. The results show that the uncertainty mainly comes from UPLC-Q/Orbitrap HRMS, standard solution preparation, recovery and random effect.
- UPLC-Q/Orbitrap HRMS,
- oral liquid,
- sildenafil derivatives,
- uncertainty,
- the expanded uncertainty

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