Determination of Chlorate and Perchlorate Amounts in Meat by High Performance Liquid Chromatography-Tandem Mass Spectrometry

YU Yanli; LI Jie

doi:10.13386/j.issn1002-0306.2022060254

Volume 44 Issue 11

May 2023

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Science and Technology of Food Industry > 2023 > 44(11): 265-270. > DOI: 10.13386/j.issn1002-0306.2022060254

YU Yanli, LI Jie. Determination of Chlorate and Perchlorate Amounts in Meat by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2023, 44(11): 265−270. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022060254.

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Determination of Chlorate and Perchlorate Amounts in Meat by High Performance Liquid Chromatography-Tandem Mass Spectrometry

YU Yanli^1, ,,
LI Jie²

1.
Shanghai Sanda University, Shanghai 201209, China
2.
Shanghai Denuo Product Service Co., Ltd., Shanghai 200436, China

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Received Date: June 27, 2022
Available Online: April 01, 2023

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Abstract

Abstract

A method for simultaneous determination of chlorate and perchlorate amounts in meat by high performance liquid chromatography-tandem mass spectrometry was developed. Samples were extracted with acetonitrile/water (13:7, V:V ) and centrifuged. The obtained supernatants were purified on a PRiME HLB solid phase extraction column, followed by an Acclaim Trinity P1 ion exchange column with the gradient elution of acetonitrile and 20 mmol/L ammonium acetate as the mobile phase to afford chlorate and perchlorate. MS/MS was performed in ESI⁺ and multiple reaction monitoring mode with an internal standard for quantification. The results showed chlorate and perchlorate had calibration curves with good linearity in the ranges of 2~200.0 ng/mL and 1~100.0 ng/mL respectively, with corresponding correlation coefficients being 0.9983 and 0.9998. The detection limits of the method were 2.0 and 1.0 μg/kg, respectively. The recovery rates of chlorate and perchlorate were 91.0%~105.7% and 94.2%~110.5% respectively, at the addition levels of 6.0, 20, 40, 200 μg/kg, and 3.0, 10, 20, 100 μg/kg. The relative standard deviations for chlorate and perchlorate were 1.8%~9.7% and 0.7%~9.4% respectively. This method would be easy to use and has high detection sensitivity and good stability, and could well meet the requirements of determining chlorate and perchlorate amounts in meat.
- chlorate,
- perchlorate,
- high performance liquid chromatography-tandem mass spectrometry,
- meat

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References

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