Optimization of Microwave Extraction of Active Ingredients Technology, Fingerprint Analysis and Anti-phytopathogenic Activity of <i>Cremastra appendiculata</i> (D. Don) Makino

YU Yixin; CHEN Longhui; CAO Dan; WANG Dong; LIU Hongxing; TANG Weizhuo

doi:10.13386/j.issn1002-0306.2022060225

Volume 44 Issue 5

Feb. 2023

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Science and Technology of Food Industry > 2023 > 44(5): 204-212. > DOI: 10.13386/j.issn1002-0306.2022060225

YU Yixin, CHEN Longhui, CAO Dan, et al. Optimization of Microwave Extraction of Active Ingredients Technology, Fingerprint Analysis and Anti-phytopathogenic Activity of Cremastra appendiculata (D. Don) Makino[J]. Science and Technology of Food Industry, 2023, 44(5): 204−212. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022060225.

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Optimization of Microwave Extraction of Active Ingredients Technology, Fingerprint Analysis and Anti-phytopathogenic Activity of Cremastra appendiculata (D. Don) Makino

College of Biological and Chemical Engineering, Changsha University, Changsha 410022, China

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Received Date: June 23, 2022
Available Online: January 02, 2023

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Abstract

Abstract

In this present paper, the optimization of microwave extraction (ME) of Cremastra appendiculata (D. Don) Makino was performed using the single factor design and response surface methodology. The ME extraction yield along with the contents of three major components of C. appendiculata Makino, including militarine, dactylorhin A, and colchicine was selected as the comprehensive indicators to evaluate the extraction process. Subsequently, the HPLC fingerprint of the above three components from 13 batches of C. appendiculata Makino was investigated. Finally, the anti-fungal activity of C. appendiculata Makino extract was evaluated by conducting a disk-diffusion method. The results demonstrated that the optimal ME technologies of C. appendiculata Makino were the ethanol concentration of 55%, the solid-liquid ratio of 1:30 g/mL, and the extraction time of 17 min. Under the above conditions, the extract yield was 12.83%. HPLC fingerprint analysis results showed that the contents of three major components displayed a consistent trend in all 13 tested samples. Militarine was found existed in 13 batches of C. appendiculata Makino, while dactylorhin A and colchicine were not detected in one sample. The results indicated a content difference in three active ingredients in C. appendiculata Makino from different areas. The anti-phytopathogenic activity results showed that the C. appendiculata Makino extract did not shown inhibitory activity against Colletotrichum gloeosporioides, Phytophthora capsica, Botrytis cinerea, Alternaria solani, Pseudomonas fluorescens, Xanthomnas oryzae, and Fusarium graminearum with their inhibition zones were less than 6 mm. However, the C. appendiculata Makino extract exhibited inhibitory activity toward to Ralstonia solanacearum with the diameter of the inhibition zone was 8.5 mm, showed antibacterial selectivity. In this study, the optimal ME technologies of C. appendiculata Makino was determined, and the established method the contents of three major components of C. appendiculata Makino was stable and feasible.These results can provide the basis for the quality control and comprehen-sive utilization of C. appendiculata Makinos.

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References

[1]	国家药典委员会. 中华人民共和国药典: 一部[M]. 北京: 中国医药科技出版社, 2015: 32 Chinese Pharmacopoeia Commission. Pharmacopoeia of the People’s Republic of China: One[M]. Beijing: China Medical Science Press, 2015: 32.
[2]	李琦, 陈宇纵, 幸海量. 山慈菇的化学成分及药理作用研究进度[J]. 药学实践杂志,2014,32:250−253. [LI Q, CHEN Y Z, XING H L. Research progress of Shancigu on chemical composition and pharmacological effects[J]. Journal of Pharmaceutical Practice,2014,32:250−253.
[3]	SHIM J S, KIM J H, LEE J, et al. Anti-angiogenic activity of a homoisoflavanone from Cremastra appendiculata[J]. Planta Medica,2004,70(2):171−173. doi: 10.1055/s-2004-815496
[4]	SUN L N. Research progress on chemical constituents and pharmacological activities of pseudo phosphorous stem of Cremastra appendiculata[J]. Chinese Traditional and Herbal Drugs,2018:5672−5682.
[5]	薛震, 李帅, 王素娟, 等. 山慈菇Cremastra appendiculata化学成分[J]. 中国中药杂志,2005,30(7):511−513. [XUE Z, LI S, WANG S J, et al. Studies on chemical constituents from the corm of Cremastra appendiculata[J]. China J Chin Mater Med,2005,30(7):511−513. doi: 10.3321/j.issn:1001-5302.2005.07.009
[6]	LIU L, LI J, ZENG K W, et al. Three new phenanthrenes from Cremastra appendiculata (D. Don) Makino[J]. Chinese Chemical Letters,2013,24(8):737−739. doi: 10.1016/j.cclet.2013.03.045
[7]	韩少伟, 王超, 崔保松, 等. 独蒜兰中丁二酸苄酯苷类化学成分的研究[J]. 中国中药杂志,2015,40(5):908−914. [HAN S W, WANG C, CUI B S, et al. Studies on glucosyloxybenzyl 2-isobutylmalates of Pleione bulbocodioides[J]. China J Chin Mater Med,2015,40(5):908−914.
[8]	郭淳, 龚永杰, 孙放, 等. 山慈菇水煎剂对人乳腺癌T-47D细胞增殖和迁移的影响[J]. 中医学报,2017,32(10):1832−1835. [GUO C, GONG Y J, SUN F, et al. Effect of iphigenia decoction on proliferation and migration of human breast cancer cell line T-47D[J]. Journal of Chinese Medicine,2017,32(10):1832−1835. doi: 10.16368/j.issn.1674-8999.2017.10.482
[9]	LIU J, HE C, TANG Y, et al. Cremastra appendiculata (D. Don) Makino, a potential anti-tumor traditional Chinese medicine[J]. Journal of Ethnopharmacology,2021:114357.
[10]	凌永梅, 谢典, 朱聪, 等. 山慈菇中总酚酸的提取工艺优化[J]. 江西科技师范大学学报,2021(6):106−110. [LING Y M, XIE D, ZHU C, et al. Optimization of extraction process of total phenolic acids from Cremastrae pseudobulbus[J]. Journal of Jiangxi Normal University of Science and Technology,2021(6):106−110. doi: 10.3969/j.issn.1007-3558.2021.06.020
[11]	王萌萌, 谢典, 周育芳, 等. 山慈菇中总黄酮的提取工艺优化[J]. 中国药房,2019,30(14):1949−1952. [WANG M M, XIE D, ZHOU Y F. Optimization of the extraction technology of total flavonoids from Typhonium divaricatum[J]. Chinese Pharmacy,2019,30(14):1949−1952.
[12]	王静, 肖秋肖, 肖红琴, 等. 响应面法优化山慈菇多糖的水提醇沉法工艺[J]. 贵州农业科学,2021,49(7):134−141. [WANG J, XIAO Q X, XIAO H Q, et al. Optimization of water-extraction and alcohol-precipitation method for Cremastra appendiculata polysaccharide by response surface method[J]. Guizhou Agricultural Sciences,2021,49(7):134−141. doi: 10.3969/j.issn.1001-3601.2021.07.022
[13]	ANI A, IOAN C, VASILE L. Microwave extraction of active principles from medicinal plants[J]. UPB Scientific Bulletin, Series B,2012,74(2):1454−2331.
[14]	DELAZAR A, NAHAR L, HAMEDEYAZDAN S, et al. Microwave-assisted extraction in natural products isolation[J]. Natural Products Isolation,2012:89−115.
[15]	LETALLIER M, BUDZINSKI H. Microwave assisted extraction of organic compounds[J]. Analusis,1999,27(3):259−270. doi: 10.1051/analusis:1999116
[16]	焦士龙. 微波提取中药有效成分实验研究[D]. 天津: 天津大学, 2006: 130 JIAO S L. Experimental study on microwave extracting efficacious components from Chinese herbal medicine[D]. Tianjin: Tianjin University, 2006, 130.
[17]	刘帅, 高丽莉, 潘丹阳. 微波辅助提取薏苡仁油工艺优化[J]. 食品工业科技,2019,40(8):145−150. [LIU S, GAO L L, PAN D Y. optimization of microwave-assisted extraction technique of cox seed oil[J]. Science and Technology of Food Industry,2019,40(8):145−150. doi: 10.13386/j.issn1002-0306.2019.08.025
[18]	ELBOUGHDIRI N. Effect of time, solvent-solid ratio, ethanol concentration and temperature on extraction yield of phenolic compounds from olive leaves[J]. Engineering, Technology & Applied Science Research,2018,8(2):2805−2808.
[19]	RADOJKOVIĆ M, ZEKOVIĆ Z, JOKIĆ S, et al. Optimization of solid-liquid extraction of antioxidants from black mulberry leaves by response surface methodology[J]. Food Technology and Biotechnology,2012,50(2):167−176.
[20]	LI J, LIU Y, FAN L, et al. Antioxidant activities of polysaccharides from the fruiting bodies of Zizyphus jujuba cv. Jinsixiaozao[J]. Carbohydrate Polymers,2011,84(1):390−394. doi: 10.1016/j.carbpol.2010.11.051
[21]	吴伟, 崔光华, 陆彬. 试验设计中多指标的优化: 星点设计和总评“归一值”的应用[J]. 中国药学杂志,2000(8):26−29. [WU W, CUI G H, LU B. Optimization of multiple variables: Application of central composite design and overall desirability[J]. Chinese Journal of Pharmaceuticals,2000(8):26−29.
[22]	文冉, 吕青涛, 李娜, 等. 基于综合加权评分-Box-Behnken响应面法优化半枝莲的提取工艺[J]. 中药材,2020,43(5):1181−1185. [WEN R, LÜ Q T, LI N, et al. Optimization of extraction process of Scutellaria barbata based on box-behnken response surface methodology combined with comprehensive weighted scoring analysis[J]. Chinese Medicinal Materials,2020,43(5):1181−1185.
[23]	唐鹏程, 王文渊, 雷干农, 等. 综合评分法优化橘皮中有效成分的提取工艺[J]. 食品研究与开发,2021,42(15):79−83. [TANG P C, WANG W Y, LEI G N, et al. Optimization of extraction technology for active components of orange peel by comprehensive scoring[J]. Food Research and Development,2021,42(15):79−83. doi: 10.12161/j.issn.1005-6521.2021.15.013
[24]	廖建良, 吴国祥, 曾令达, 等. 沉香提取物的抑菌活性[J]. 江苏农业科学,2013,41(6):285−287. [LIAO J L, WU G X, ZENG L D, et al. Antibacterial activity of agarwood extract[J]. Jiangsu Agricultural Sciences,2013,41(6):285−287. doi: 10.3969/j.issn.1002-1302.2013.06.105
[25]	崔保松, 宋杰, 李帅, 等. RP-HPLC同时测定山慈菇药材中dactylorhin A和militarine的含量[J]. 中国中药杂志,2013,38(24):4347−4350. [CUI B S, SONG J, LI S, et al. Determination of dactylorhin A and militarine in three varieties of Cremastrae pseudobulbus/Pleiones pseudobulbus by HPLC[J]. China J Chin Mater Med,2013,38(24):4347−4350.
[26]	吕林锋, 蒋永海, 朱希, 等. 山慈菇HPLC指纹图谱质量评价[J]. 中国现代应用药学,2016,33(10):1276−1280. [LÜ L F, JIANG Y H, ZHU X, et al. Study on HPLC fingerprints of Cremastrae pseudobulbus pleiones pseudobulbus[J]. Chin J Mod Appl Pharm,2016,33(10):1276−1280. doi: 10.13748/j.cnki.issn1007-7693.2016.10.013
[27]	管伦兴, 储益平. 云南丽江山慈菇品种考证及有效成分秋水仙碱含量的研究[J]. 中药与临床,2015,6(3):1−3. [GUAN L X, CHU Y P. Species textual researchand study on colchicines content of Shancigu in Lijiang of Yunnan[J]. Chin J New Drugs Clin Rem,2015,6(3):1−3.