Effects of Theabrownin on Brain<italic/> Metabolites in<italic/> D-galactose-induced Aging in Mice

LEI Shuwen; XIE Guihua; ZHANG Zhifang; MIAO Yue; ZHAO Chunyan; CHEN Dehong; HOU Yan; GONG Jiashun

doi:10.13386/j.issn1002-0306.2022050204

Volume 44 Issue 5

Feb. 2023

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Science and Technology of Food Industry > 2023 > 44(5): 43-52. > DOI: 10.13386/j.issn1002-0306.2022050204

LEI Shuwen, XIE Guihua, ZHANG Zhifang, et al. Effects of Theabrownin on Brain Metabolites in D-galactose-induced Aging in Mice[J]. Science and Technology of Food Industry, 2023, 44(5): 43−52. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022050204.

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Effects of Theabrownin on Brain Metabolites in D-galactose-induced Aging in Mice

1.
College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China
2.
College of Tea, Yunnan Agricultural University, Kunming 650201, China
3.
College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China

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Received Date: May 18, 2022
Available Online: December 29, 2022

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Abstract

Abstract

The objective of the study is to investigate the effect of theabrownin on the immunohistochemistry of brain tissue in aging mice, screen the differential metabolites and analyze the metabolic pathways involved. A mouse aging model was established by subcutaneous injection of D-galactose in mice. Immunohistochemical and UHPLC-QE-MS metabolomics techniques were used to detect brain samples of normal control mice, D-galactose model mice and theabrownin-treated (pretreatment and treatment) mice. The results showed that the areal density of choline acetyltransferase positive expression was significantly increased (P<0.05) after theabrownin intervention. Prophylactic administration of theabrownin could significantly regulate 17 metabolites in brain tissue of aging mice, mainly involving glycerophospholipid metabolism, histidine metabolism, TCA cycle, glyoxylate and dicarboxylate metabolism and beta-alanine metabolism. Theabrownin treatment could significantly regulate 32 metabolites in brain tissue of aging mice, mainly involving glycerophospholipid metabolism, histidine metabolism, taurine and hypotaurine metabolism, valine, leucine and isoleucine degradation, phenylalanine, tyrosine and tryptophan biosynthesis. Carnosine, LysoPC(P-18:1(9Z)), PS(20:5(5Z,8Z,11Z,14Z,17Z)/18:1(9Z)), PC(15:0/15:0), D-arabinose 5-phosphate, cholesterol sulfate, adenosine diphosphate ribose, glycerophospholipid metabolism and histidine metabolism were common in the theabrownin pretreatment and theabrownin treatment groups. Theabrownin can play an anti-aging role by regulating lipid metabolism, energy metabolism, amino acid metabolism and other processes in mice.
- theabrownin,
- immunocytochemistry,
- brain metabonomics,
- anti-aging

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