Simultaneous Determination of Five Phenolic Acids and Isoquercitrin in Sweet Potato (<i>Ipomoea batatas</i> (L.) Lam) Leaves by HPLC

FU Zhifeng; ZHOU He; SHI Yan; LU Jianwen

doi:10.13386/j.issn1002-0306.2022050099

Volume 44 Issue 5

Feb. 2023

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Science and Technology of Food Industry > 2023 > 44(5): 292-298. > DOI: 10.13386/j.issn1002-0306.2022050099

FU Zhifeng, ZHOU He, SHI Yan, et al. Simultaneous Determination of Five Phenolic Acids and Isoquercitrin in Sweet Potato (Ipomoea batatas (L.) Lam) Leaves by HPLC[J]. Science and Technology of Food Industry, 2023, 44(5): 292−298. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022050099.

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Simultaneous Determination of Five Phenolic Acids and Isoquercitrin in Sweet Potato (Ipomoea batatas (L.) Lam) Leaves by HPLC

1.
Food and Drug Inspection Sub Center of Jiujiang Inspection, Testing and Certification Center, Jiujiang 332000, China
2.
Stare Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
3.
Selenium Resources Development and Utilization Center of Yichun, Yichun 336000, China

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Received Date: May 10, 2022
Available Online: January 10, 2023

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Abstract

Abstract

In this study, a reversed-phase high performance liquid-chromatography coupled to ultraviolet detection (RP-HPLC/UV) method for simultaneous determination of six major bioactive constituents (caffeic acid, chlorogenic acid, 3,4-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid, 4,5-dicaffeoyl quinic acid and isoquercetin) in sweet potato leaves was developed and validated after optimization of various chromatographic conditions and other experimental parameters. The chromatographic separation was achieved on an Agilent ZORBAX SB-C₁₈ reserved-phase column (4.6 mm×250 mm, 5 μm) using a gradient elution program with a mobile phase consisting of 0.1% formic acid aqueous solution (solvent A) and acetonitrile (solvent B) (0~12 min, 10%~25% B; 12~18 min, 25% B), at a flow rate of 0.8 mL/min, an operating temperature of 30 ℃, and a wavelength of 326 nm. The calibration curves were linear in the range of 0.25~25.0, 0.5~50.0, 5.0~100.0, 10.0~200.0, 5.0~100.0, 0.5~50.0 μg/mL for caffeic acid, chlorogenic acid, 3,4-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid, 4,5-dicaffeoyl quinic acid and isoquercetin, respectively. The present method was fast (18 min), and demonstrated acceptable values for linearity (R²≥0.998), stability (RSD≤1.97%), recovery (95.14%~103.22%) and precision (RSD≤1.56%). Additionally, the new method was successfully applied to determine components in sweet potato leaves from ten different regions of Jiangxi Province, and the results indicated that the sample harvested in Fengcheng city of Yichun contained higher total phenol content than those collected in other regions.
- HPLC,
- caffeoylquinic acids,
- isoquercitrin,
- simultaneous determination,
- sweet potato leaves

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References (33)

References

[1]	NGUYEN H C, CHEN C C, LIN K H, et al. Bioactive compounds, antioxidants, and health benefits of sweet potato leaves[J]. Molecules,2021,26(7):1820. doi: 10.3390/molecules26071820
[2]	JANG Y, KOH E. Antioxidant content and activity in leaves and petioles of six sweet potato (Ipomoea batatas L.) and antioxidant properties of blanched leaves[J]. Food Science and Biotechnology,2019,28(2):337−345. doi: 10.1007/s10068-018-0481-3
[3]	TAIRA J, UEHARA M, TSUCHIDA E, et al. Inhibition of the β-catenin/Tcf signaling by caffeoylquinic acids in sweet potato leaf through down regulation of the Tcf-4 transcription[J]. Journal of Agricultural and Food Chemistry,2014,62(1):167−172. doi: 10.1021/jf404411r
[4]	LUO D, MU T H, SUN H N. Profiling of phenolic acids and flavonoids in sweet potato (Ipomoea batatas L.) leaves and evaluation of their anti-oxidant and hypoglycemic activities[J]. Food Bioscience,2021,39:100801. doi: 10.1016/j.fbio.2020.100801
[5]	NTCHAPDA F, TCHATCHOUANG F C, MIAFFO D, et al. Hypolipidemic and anti-atherosclerogenic effects of aqueous extract of Ipomoea batatas leaves in diet-induced hypercholesterolemic rats[J]. Journal of Integrative Medicine,2021,19(3):243−250. doi: 10.1016/j.joim.2021.02.002
[6]	ISHIDA H, SUZUNO H, SUGIYAMA N, et al. Nutritive evaluation on chemical components of leaves, stalks and stems of sweet potatoes (Ipomoea batatas poir)[J]. Food Chemistry,2000,68(3):359−367. doi: 10.1016/S0308-8146(99)00206-X
[7]	CHEN Y, FU Z F, TU Z C, et al. Influence of in vitro gastrointestinal digestion on the bioavailability and antioxidant activity of polyphenols from Ipomoea batatas leaves[J]. International Journal of Food Science & Technology,2017,52(5):1131−1137.
[8]	ZHANG C C, LIU D Q, WU L H, et al. Chemical characterization and antioxidant properties of ethanolic extract and its fractions from sweet potato (Ipomoea batatas L.) leaves[J]. Foods,2019,9(1):15. doi: 10.3390/foods9010015
[9]	NAVEED M, HEJAZI V, ABBAS M, et al. Chlorogenic acid (CGA): A pharmacological review and call for further research[J]. Biomedicine & Pharmacotherapy,2018,97:67−74.
[10]	CHEN S, LIU J, DONG G Q, et al. Flavonoids and caffeoylquinic acids in Chrysanthemum morifolium Ramat flowers: A potentially rich source of bioactive compounds[J]. Food Chemistry,2021,344:128733. doi: 10.1016/j.foodchem.2020.128733
[11]	LIAN W W, DU G H. Caffeic acid[M]//Natural small molecule drugs from plants. Springer, Singapore, 2018: 19−23.
[12]	LI C, TAN F, YANG J J, et al. Antioxidant effects of Apocynum venetum tea extracts on D-galactose-induced aging model in mice[J]. Antioxidants,2019,8(9):381. doi: 10.3390/antiox8090381
[13]	STASZOWSKA-KARKUT M, MATERSKA M. Phenolic composition, mineral content, and beneficial bioactivities of leaf extracts from black currant (Ribes nigrum L.), raspberry (Rubus idaeus), and aronia (Aronia melanocarpa)[J]. Nutrients,2020,12(2):463. doi: 10.3390/nu12020463
[14]	EL-HAWARY S S, MOHAMMED R, EL-DIN M E, et al. Comparative phytochemical analysis of five Egyptian strawberry cultivars (Fragaria×ananassa Duch.) and antidiabetic potential of festival and red merlin cultivars[J]. RSC Advances,2021,11(27):16755−16767. doi: 10.1039/D0RA10748D
[15]	FU Z F, TU Z C, ZHANG L, et al. Antioxidant activities and polyphenols of sweet potato (Ipomoea batatas L.) leaves extracted with solvents of various polarities[J]. Food Bioscience,2016,15:11−18. doi: 10.1016/j.fbio.2016.04.004
[16]	WAY M L, JONES J E, NICHOLS D S, et al. A comparison of laboratory analysis methods for total phenolic content of cider[J]. Beverages,2020,6(3):55. doi: 10.3390/beverages6030055
[17]	BAE I K, HAM H M, JEONG M H, et al. Simultaneous determination of 15 phenolic compounds and caffeine in teas and mate using RP-HPLC/UV detection: Method development and optimization of extraction process[J]. Food Chemistry,2015,172:469−475. doi: 10.1016/j.foodchem.2014.09.050
[18]	RUSSO M, FANALI C, TRIPODO G, et al. Analysis of phenolic compounds in different parts of pomegranate (Punica granatum) fruit by HPLC-PDA-ESI/MS and evaluation of their antioxidant activity: Application to different Italian varieties[J]. Analytical and Bioanalytical Chemistry,2018,410(15):3507−3520. doi: 10.1007/s00216-018-0854-8
[19]	MUSTAFA A M, ANGELONI S, ABOUELENEIN D, et al. A new HPLC-MS/MS method for the simultaneous determination of 36 polyphenols in blueberry, strawberry and their commercial products and determination of antioxidant activity[J]. Food Chemistry,2022,367:130743. doi: 10.1016/j.foodchem.2021.130743
[20]	钟伟. 红薯叶中多酚类物质的抗氧化及抗肿瘤细胞增殖作用研究[D]. 广州: 华南理工大学, 2015 ZHONG W. Research evaluation of antioxidant and antitumour activities of Ipomoea batatas leaves[D]. Guangzhou: South China University of Technology, 2015
[21]	Q2 (R1) Validation of analytical procedures: Text and methodology[S].2005.
[22]	覃日宏, 陈汝旭, 盘涌, 等. HPLC法同时测定五指毛桃中6个黄酮类成分的含量[J]. 药物分析杂志,2020,40(12):2244−2249. [QIN R H, CHEN R X, PAN Y, et al. Simultaneous determination of six flavonoids in Ficushirta Vahl. by HPLC[J]. Chinese Journal of Pharmaceutical Analysis,2020,40(12):2244−2249. doi: 10.16155/j.0254-1793.2020.12.19
[23]	TSOLMON B, FANG Y, YANG T, et al. Structural identification and UPLC-ESI-QTOF-MS-MS analysis of flavonoids in the aquatic plant Landoltia punctata and their in vitro and in vivo antioxidant activities[J]. Food Chemistry,2021,343:128392. doi: 10.1016/j.foodchem.2020.128392
[24]	LIU J, MU T H, SUN H N, et al. Optimization of ultrasonic-microwave synergistic extraction of flavonoids from sweet potato leaves by response surface methodology[J]. Journal of Food Processing and Preservation,2019,43(5):e13928. doi: 10.1111/jfpp.13928
[25]	马帅, 王纪华, 高媛, 等. 超高效液相色谱-串联质谱法同时测定5个产地花椰菜和西兰花中的23种酚酸类化合物[J]. 食品科学,2018,39(4):176−187. [MA S, WANG J H, GAO Y, et al. Simultaneous determination of twenty-three phenolic acids in Cauliflower (Brassica oleracea L. var. botrytis L.) and Broccoli (B. oleracea L. var. italica) from five producing places by ultra performance liquid chromatography-tandem mass spectrometry[J]. Food Science,2018,39(4):176−187. doi: 10.7506/spkx1002-6630-201804027
[26]	ZHANG A, WAN L, WU C Y, et al. Simultaneous determination of 14 phenolic compounds in grape canes by HPLC-DAD-UV using wavelength switching detection[J]. Molecules,2013,18(11):14241−14257. doi: 10.3390/molecules181114241
[27]	HUANG R T, LU Y F, INBARAJ B S, et al. Determination of phenolic acids and flavonoids in Rhinacanthus nasutus (L.) kurz by high-performance-liquid-chromatography with photodiode-array detection and tandem mass spectrometry[J]. Journal of Functional Foods,2015,12:498−508. doi: 10.1016/j.jff.2014.12.002
[28]	段云飞, 吴光斌, 叶洪, 等. HPLC法同时测定采后莲雾果实7种有机酸的含量[J]. 食品科学,2021,42(4):175−180. [DUAN Y F, WU G B, YE H, et al. Simultaneous determination of seven organic acids in wax apple (Syzygium samarangenese [Blume] Merrill & L. M. Perry) fruit during postharvest storage by high performance liquid chromatography[J]. Food Science,2021,42(4):175−180. doi: 10.7506/spkx1002-6630-20191025-287
[29]	朱亚珠. 甘薯叶中咖啡酰奎尼酸类物质的分离纯化和高效液相色谱法分析[J]. 食品工业科技,2015,36(10):73−77. [ZHU Y Z. Purification of caffeoylquinic acids from sweet potato leaves and their analysis by high performance liquid chromatography[J]. Science and Technology of Food Industry,2015,36(10):73−77. doi: 10.13386/j.issn1002-0306.2015.10.006
[30]	MAKORI S I, MU T H, SUN H N. Total polyphenol content, antioxidant activity, and individual phenolic composition of different edible parts of 4 sweet potato cultivars[J]. Natural Product Communications, 2020, 15(7): 1934578X20936931.
[31]	SUÁREZ S, MU T H, SUN H N, et al. Antioxidant activity, nutritional, and phenolic composition of sweet potato leaves as affected by harvesting period[J]. International Journal of Food Properties,2020,23(1):178−188. doi: 10.1080/10942912.2020.1716796
[32]	XIANG G, YANG H Y, YANG L, et al. Multivariate statistical analysis of tobacco of different origin, grade and variety according to polyphenols and organic acids[J]. Microchemical Journal,2010,95(2):198−206. doi: 10.1016/j.microc.2009.12.001
[33]	ZHANG D Y, WAN Y, HAO J Y, et al. Evaluation of the alkaloid, polyphenols, and antioxidant contents of various mulberry cultivars from different planting areas in eastern China[J]. Industrial Crops and Products,2018,122:298−307. doi: 10.1016/j.indcrop.2018.05.065