Determination of 37 Kinds of Fatty Acids in Infant Milk Powder by Gas Chromatography-Hydrogen Flame Ionization Detector

LUO Yeli; LI Guowei; DONG Mei; ZHAO Bi; QIANG Xin

doi:10.13386/j.issn1002-0306.2022040281

Volume 44 Issue 11

May 2023

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Science and Technology of Food Industry > 2023 > 44(11): 245-253. > DOI: 10.13386/j.issn1002-0306.2022040281

LUO Yeli, LI Guowei, DONG Mei, et al. Determination of 37 Kinds of Fatty Acids in Infant Milk Powder by Gas Chromatography-Hydrogen Flame Ionization Detector[J]. Science and Technology of Food Industry, 2023, 44(11): 245−253. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022040281.

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Determination of 37 Kinds of Fatty Acids in Infant Milk Powder by Gas Chromatography-Hydrogen Flame Ionization Detector

1.
Weinan Inspection and Research Institute, Weinan 714000, China
2.
Fuping County Inspection and Testing Center (Shaanxi Sheep Milk Product Quality Supervision and Inspection Center), Fuping 711700, China

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Received Date: April 24, 2022
Available Online: April 05, 2023

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Abstract

Abstract

Objective: To establish an analytical method for the determination of 37 kinds of fatty acid in milk powder by gas chromatography-hydrogen flame ionization detector (GC-FID). Methods: Infant milk powder samples were methylated by acetyl chloride-methanol method (10% acetyl chloride methanol solution in water bath at 80 ℃±1 ℃ for 2 h). The supernatant was collected by centrifugation, passed through a 0.22 μm organic filter membrane, and separated through SP-2560 (100 m×250 μm×0.2 μm). Then, the detection was operated by gas chromatography, and the external standard method was used for quantification. Results: The linearity of 37 kinds of fatty acid methyl esters was good in the concentration range of 0.002~0.2 mg/mL, and the correlation coefficients were all greater than 0.999. The recovery rate fell in 85.5%~100.0% for the low gradient, 91.2%~104.2% for the medium gradient, and 86.8%~103.8% for the high gradient. The precision was between 0.88% and 3.81%, and the actual sample determination results met the relevant standards. Conclusion: This method is a more rapid and better separation degree of fatty acid detection technology established on the basis of national standard, which has good practical value. The method has good stability, low cost, good reproducibility and high accuracy, and it is suitable for the determination of 37 kinds of fatty acid in infant milk powder.
- fatty acid,
- acetyl chloride-methanol method,
- gas chromatography,
- infant milk powder

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