Proteomics Analysis of the Effect of Uracil on Pullulan Polysaccharide Production by <i>Aureobasidium pullulans</i>

CHEN Shiwei; TANG Shuxian; WANG Genan; WU Chengyuan; ZHANG Shuhui; ZHAO Tingbin; YIN Haisong; ZHENG Zhiqiang; QIAO Changsheng

doi:10.13386/j.issn1002-0306.2021120231

Volume 43 Issue 16

Aug. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(16): 18-25. > DOI: 10.13386/j.issn1002-0306.2021120231

CHEN Shiwei, TANG Shuxian, WANG Genan, et al. Proteomics Analysis of the Effect of Uracil on Pullulan Polysaccharide Production by Aureobasidium pullulans[J]. Science and Technology of Food Industry, 2022, 43(16): 18−25. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120231.

Citation:

PDF (7608 KB)

Proteomics Analysis of the Effect of Uracil on Pullulan Polysaccharide Production by Aureobasidium pullulans

1.
College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China
2.
Tianjin Huizhi Baichuan Bioengineering Co., Ltd., Tianjin 300457, China
3.
College of Bioengineering, Tianjin Modern Vocational Technology College, Tianjin 300457, China
4.
Institute of Systems Engineering, Academy of Military Sciences, Beijing 101300, China
5.
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education and Tianjin Key Laboratory of Industrial Microbiology, Tianjin 300457, China
6.
Tianjin Engineering Center of Microbial Metabolism and Fermentation Process Control, Tianjin 300457, China

More Information

Received Date: December 20, 2021
Available Online: June 12, 2022

Graphical Abstract

Abstract

Abstract

In order to understand the mechanism of uracil on the growth by Aureobasidium pullulans and synthesis of pullulan, the optimal dosage and time of uracil in pullulan polysaccharide fermentation were studied. The label-free quantitative method and liquid chromatography-tandem mass spectrometry were used to compare the protein components in the late fermentation stage (88 h) by Aureobasidium pullulans and the differential proteins were analyzed by bioinformatics. The results showed that adding 0.5g/L uracil at 48 h had the most significant increasing in pullulan polysaccharide yield, which was verified on 5 L fermenter from 70.13 to 86.27 g/L, increasing by 23%. Protein component analysis identified 80 different proteins, including 40 increases protein and 40 lower protein (difference>2, P<0.05), the protein of the differences of clustering analysis, GO function enrichment, KEGG pathway analysis showed that the differences in protein involved in cellular processes, metabolic processes, and other important biological processes. The differential proteins were mainly involved in glycolysis, metabolism of fructose and mannose, metabolism of glyoxylic acid and dicarboxylic acid, pyruvate metabolism and TCA cycle, which ultimately led to the change of pullulan polysaccharide yield. These results would provide a molecular basis for further understanding the metabolic mechanism of pullulan polysaccharides produced by Aureobasidium pullulans.
- pllulan,
- uacil,
- proteomics,
- luid chromatography-tandem mass spectrometry

FullText(HTML)

References (23)

References

[1]	GIBBS P A, SEVIOUR R J. Pullulan, polysaccharides in medicinal applications[J]. Carbohydrate Polymers,1996:59−86.
[2]	LEATHERS T D. Polysaccharides Ⅱ: Polysaccharides from eukaryotes[M]. 2002, 6: 1-35.
[3]	PETROV P, SHINGEL K, SCRIPKO A, et al. The biosynthesis of pullulan by strain BMP-97 of Aureobasidium pullulans[J]. Biotekhnologiya,2002:36−48.
[4]	MERT H, ÖZKAHRAMAN B, DAMAR H. A novel wound dressing material: Pullulan grafted copolymer hydrogel via UV copolymerization and crosslinking[J]. Journal of Drug Delivery Science and Technology,2020,60:101962. doi: 10.1016/j.jddst.2020.101962
[5]	HAN K, LIU Y, LIU Y, et al. Characterization and film-forming mechanism of egg white/pullulan blend film[J]. Food Chem,2020,315:126201. doi: 10.1016/j.foodchem.2020.126201
[6]	KRAŚNIEWSKA K, POBIEGA K, GNIEWOSZ M. Pullulan-biopolymer with potential for use as food packaging[J]. International Journal of Food Engineering, 2019, 15(9).
[7]	SINGH R S, KAUR N, HASSAN M, et al. Pullulan in biomedical research and development-a review[J]. Int J Biol Macromol,2021,166:694−706. doi: 10.1016/j.ijbiomac.2020.10.227
[8]	CARVER J D, WALKER W A. The role of nucleotides in human nutrition[J]. The Journal of Nutritional Biochemistry,1995,6(2):58−72. doi: 10.1016/0955-2863(94)00019-I
[9]	WEST T P. Pyrimidine base supplementation effects curdlan production in Agrobacterium sp. ATCC 31749[J]. J Basic Microbiol,2006,46(2):153−157. doi: 10.1002/jobm.200510067
[10]	LEE J S, WEE J W, LEE H Y, et al. Effects of ascorbic acid and uracil on exo-polysaccharide production with Hericium erinaceus in liquid culture[J]. Biotechnology and Bioprocess Engineering,2010,15(3):453−459. doi: 10.1007/s12257-008-0265-3
[11]	SALEH N E, ABDELKHALEK N, LASHEN S, et al. Dietary inosine and uracil efficacy on immune and growth genes expression, physiological performance and intestinal topography of juvenile sea bream (Sparus aurata)[J]. Aquaculture Research,2021,52(8):3935−3948. doi: 10.1111/are.15237
[12]	SHENG L, ZHU G, TONG Q. Effect of uracil on pullulan production by Aureobasidium pullulans CGMCC1234[J]. Carbohydr Polym,2014,101:435−437. doi: 10.1016/j.carbpol.2013.09.063
[13]	张攀, 张伊凡, 成刚刚, 等. 基于代谢组学分析MgSO₄·7H₂O和K₂HPO₄协同对普鲁兰多糖生物合成的影响[J]. 食品科技,2021,46(4):1−8. [ZHANG P, ZHANG Y F, CHENG G G, et al. Analysis of the effect of MgSO₄·7H₂O and K₂HPO₄ on pullulan biosynthesis based on metabolomics[J]. Food Science,2021,46(4):1−8. ZHANG P, ZHANG Y F, CHENG G G, et al. Analysis of the effect of MgSO₄·7H₂O and K₂HPO₄ on pullulan biosynthesis based on metabolomics[J]. Food Science, 2021, 46(4): 1-8.
[14]	谈梦飞, 高谦, 王萌, 等. Tween-60对出芽短梗霉合成多糖代谢组学的分析[J]. 食品科技,2018,43(9):1−6. [TAN M F, GAO Q, WANG M, et al. Metabolomics analysis of Tween-60 on pullulan by Aureobsidium pullulans[J]. Food Science,2018,43(9):1−6. TAN M F, GAO Q, WANG M, et al. Metabolomics analysis of Tween-60 on pullulan by Aureobsidium pullulans[J]. Food Science, 2018, 43(9): 1-6.
[15]	JACEK R W, ALEXANDRE Z, NAGARJUNA N, et al. Universal sample preparation method for proteome analysis[J]. Nat Methods,2009,6(5):359−362. doi: 10.1038/nmeth.1322
[16]	ZHOU D, ZHANG Q, LI P, et al. Combined transcriptomics and proteomics analysis provides insight into metabolisms of sugars, organic acids and phenols in UV-C treated peaches during storage[J]. Plant Physiol Biochem,2020,157:148−159. doi: 10.1016/j.plaphy.2020.10.022
[17]	COX J, MANN M. MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification[J]. Nat Biotechnol,2008,26(12):1367−1372. doi: 10.1038/nbt.1511
[18]	WILLIAMS E G, WU Y, JHA P, et al. Systems proteomics of liver mitochondria function[J]. Science,2016,352(6291):aad0189. doi: 10.1126/science.aad0189
[19]	GUO H, WANG L, DENG Y, et al. Novel perspectives of environmental proteomics[J]. Sci Total Environ,2021,788:147588. doi: 10.1016/j.scitotenv.2021.147588
[20]	VAN S E. Hexokinase/glucokinase[J]. Encyclopedia of Biological Chemistry, 2013: 543−547.
[21]	WEI X, LIU G L, JIA S L, et al. Pullulan biosynthesis and its regulation in Aureobasidium spp[J]. Carbohydrate Polymers,2021:251.
[22]	RYAN D G, FREZZA C, O'NEILL L A. TCA cycle signalling and the evolution of eukaryotes[J]. Curr Opin Biotechnol,2021,68:72−88.
[23]	XUE J, LI T, CHEN T T, et al. Regulation of malate-pyruvate pathway unifies the adequate provision of metabolic carbon precursors and NADPH in Tetradesmus obliquus[J]. Algal Research,2021:57.

Supplements (0)

Cited By

Cited by

Periodical cited type(2)

1.	谭婉静，张笑薇，白泓，李晓凤，余以刚，肖性龙. 丁香油与月桂酸对金黄色葡萄球菌的协同抑制作用. 现代食品科技. 2022(02): 278-285+265 .
2.	于航，王海默，钟世勋，吕月琴，刘兆胜，孙汝江. 抗菌肽APSH-07与抗生素对常见致病菌的体外协同抗菌效果研究. 饲料工业. 2021(16): 22-26 .