Exploring the Intervention Effects of Mulberry Polyphenols on Nonylphenol-Induced Neurobehavioral Toxicity Based on Thyroid Hormone and Deiodinase

LI Zhongyi; KE Qianhua; LIU Huan; HAN Wenna; ZHU Siyu; LIU Chunhong

doi:10.13386/j.issn1002-0306.2021120223

Volume 43 Issue 18

Sep. 2022

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Science and Technology of Food Industry > 2022 > 43(18): 390-398. > DOI: 10.13386/j.issn1002-0306.2021120223

LI Zhongyi, KE Qianhua, LIU Huan, et al. Exploring the Intervention Effects of Mulberry Polyphenols on Nonylphenol-Induced Neurobehavioral Toxicity Based on Thyroid Hormone and Deiodinase[J]. Science and Technology of Food Industry, 2022, 43(18): 390−398. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120223.

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Exploring the Intervention Effects of Mulberry Polyphenols on Nonylphenol-Induced Neurobehavioral Toxicity Based on Thyroid Hormone and Deiodinase

LI Zhongyi^{1, 2,},
KE Qianhua^{1, 2},
LIU Huan^{1, 2},
HAN Wenna^{1, 2},
ZHU Siyu^{1, 2},
LIU Chunhong^{1, 2, ,}

1.
College of Food Science, South China Agricultural University, Guangzhou 510642, China
2.
Key Laboratory of Food Quality and Safety in Guangdong Province, Guangzhou 510642, China

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Received Date: December 20, 2021
Available Online: July 17, 2022

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Abstract

Abstract

The purpose of this study was to explore the intervention effects of mulberry polyphenols on neurobehavioral toxicity induced by nonylphenol (NP). 70 SPF male SD rats were randomly divided into seven groups: Blank control group (corn oil+saline), Mulberry control group [corn oil+mulberry crude extract (MCE)], NP exposure group (NP+saline), mulberry group (NP+MCE), anthocyanin group (NP+anthocyanin), resveratrol group (NP+resveratrol), combined group (NP+anthocyanin+resveratrol). The gavage amount of MCE was calculated according to 120 mg/kg·bw polyphenol content, and the anthocyanin and resveratrol content corresponded to the content in MCE. Open field test (OFT) and elevated plus maze (EPM) were used to evaluate the intervention effects of mulberry polyphenols on neurobehavioral toxicity of NP, and the levels of thyroxine (FT3, FT4), thyroid-stimulating hormone (TSH) and deiodinase (DIO1, DIO2, DIO3) were measured. The results showed that compared with NP group, all four intervention groups had varying degrees of intervention effects on the decrease of body weight growth rate, food utilization rate and organ coefficient of rats induced by NP (P<0.05 or P<0.01). The total distance of movement in OFT, the times of entering the central area, the total time of entering the open arm and staying time in EPM were significantly higher in the intervention groups than those in the NP group (P<0.05 or P<0.01), the levels of FT3, FT4 and TSH in serum increased significantly (P<0.05), while the content of deiodinase in liver decreased but had no significant difference. In summary: The MCE, anthocyanin and resveratrol could improve the neurobehavioral toxicity induced by NP, which might be related to the regulation of thyroid hormone, thyrotropin and deiodinase.
- nonylphenol,
- mulberry polyphenol,
- thyroid hormone,
- open field test,
- elevated cross maze

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[1]	CAPELA D, POISSENOT K, DOMBRET C, et al. Effects of combined exposure of adult male mice to di-(2-ethylexyl)phthalate and nonylphenol on behavioral and neuroendocrine responses[J]. Chemosphere,2019,221:573−582. doi: 10.1016/j.chemosphere.2019.01.071
[2]	王晨. 商务部公告了对原产于印度和台湾地区的进口壬基酚反倾销措施期终复审裁定[J]. 精细与专用化学品,2019,27(4):8. [WANG C. Ministry of commerce announces final review of anti-dumping measures against imports of nonylphenol originating in India and Taiwan[J]. Fine and Specialty Chemicals,2019,27(4):8. WANG C. Ministry of Commerce Announces Final Review of anti-dumping measures against imports of nonylphenol originating in India and Taiwan[J]. Fine and Specialty Chemicals, 2019, 27(4): 8.
[3]	陈慰双. 我国水环境中壬基酚的污染现状及生态风险评估[D]. 青岛: 中国海洋大学, 2013. CHEN W S. Pollution status and ecological risk assessment of nonylphenol in water environment in China[D]. Qingdao: Ocean University of China, 2013.
[4]	殷伟, 范德玲, 汪贞, 等. 天津市地表水体与沉积物中7种高关注酚类化合物的污染特征与生态风险分析[J]. 生态毒理学报,2020,15(1):230−241. [YIN W, FAN D L, WANG Z, et al. Pollution characteristics and ecological risk analysis of 7 highly concerned phenolic compounds in surface water and sediments in Tianjin[J]. Journal of Ecotoxicology,2020,15(1):230−241. doi: 10.7524/AJE.1673-5897.20190125002 YIN W, FAN D L, WANG Z, et al. Pollution characteristics and ecological risk analysis of 7 highly concerned phenolic compounds in surface water and sediments in Tianjin[J]. Journal of Ecotoxicology, 2020, 15(1): 230-241. doi: 10.7524/AJE.1673-5897.20190125002
[5]	HONG Y J, FENG C L, YAN Z F, et al. Nonylphenol occurrence, distribution, toxicity and analytical methods in freshwater[J]. Environmental Chemistry Letters,2020,18(6):2095−2106. doi: 10.1007/s10311-020-01060-3
[6]	陈益欧. 苏州市主要水体、饮用水及餐饮用具壬基酚污染状况调查及其去除方法研究[D]. 苏州: 苏州大学, 2013. CHEN Y O. Investigation and removal method of nonylphenol pollution in main water, drinking water and catering utensils in Suzhou[D]. Suzhou: Suzhou University, 2013.
[7]	孙沛雯, 王中卫, 李翔宇, 等. 不同水体中环境类雌激素污染状况调查与分析[J]. 干旱环境监测,2020,34(1):44−48. [SUN P W, WANG Z W, LI X G, et al. Investigation and analysis of environmental estrogenic pollution in different water bodies[J]. Drought Environment Monitoring,2020,34(1):44−48. doi: 10.3969/j.issn.1007-1504.2020.01.010 SUN P W, WANG Z W, LI X G, et al. Investigation and analysis of environmental estrogenic pollution in different water bodies[J]. Drought Environment Monitoring, 2020, 34(1): 44-48. doi: 10.3969/j.issn.1007-1504.2020.01.010
[8]	SHELLEY L K, ROSS P S, MILLER K M, et al. Toxicity of atrazine and nonylphenol in juvenile rainbow trout (Oncorhynchus mykiss): Effects on general health, disease susceptibility and gene expression[J]. Aquat Toxicol,2012,217(26):124−125.
[9]	MIEGE C, PERETTI A, LABADIE P, et al. Occurrence of priority and emerging organic compounds in fishes from the Rhone River (France)[J]. Anal Bioanal Chem,2012,404(9):2721−2735. doi: 10.1007/s00216-012-6187-0
[10]	LIU C, SUN Y K, SONG Y T, et al. Nonylphenol diethoxylate inhibits apoptosis induced in PC12 cells[J]. Environmental Toxicology,2016,31(11):1389−1398. doi: 10.1002/tox.22144
[11]	ARONZON C M, SVARTZ G V, COLL C S P. Synergy between diazinon and nonylphenol in toxicity during the early development of the Rhinella arenarum Toad[J]. Water Air and Soil Pollution,2016,227(5):139−149. doi: 10.1007/s11270-016-2799-x
[12]	DWIVEDI S, DSOUZA L C, SHETTY N G, et al. Hsp27, a potential EcR target, protects nonylphenol-induced cellular and organismal toxicity in Drosophila melanogaster[J]. Environmental Pollution,2022,293:118484. doi: 10.1016/j.envpol.2021.118484
[13]	许洁. 环境雌激素壬基酚对仔鼠神经发育毒性及机制研究[D]. 重庆: 重庆医科大学, 2012. XU J. Study on neurodevelopmental toxicity and mechanism of environmental estrogen nonylphenol in offspring rats[D]. Chongqing: Chongqing Medical University, 2012.
[14]	王林. 环境浓度NP长期暴露对雄性大鼠甲状腺功能与增生性病变的影响及锌硒茶干预研究[D]. 遵义: 遵义医科大学, 2020. WANG L. Effects of long-term exposure to environmental concentration of NP on thyroid function and proliferative lesions in male rats and intervention of zinc-selenium tea[D]. Zunyi: Zunyi Medical University, 2020.
[15]	ARAMWIT P, BANG N, SRICHANA T. The properties and stability of anthocyanins in mulberry fruits[J]. Food Research International,2010,43(4):1093−1097. doi: 10.1016/j.foodres.2010.01.022
[16]	SHIN S K, YOO J M, LI F Y, et al. Mulberry fruit improves memory in scopolamine-treated mice: Role of cholinergic function, antioxidant system, and TrkB/Akt signaling[J]. Nutritional Neuroscience,2019,24(12):940−950.
[17]	TSURUDOME N, MINAMI Y, KAJIYA K. Fisetin, a major component derived from mulberry (Morus australis Poir. ) leaves, prevents vascular abnormal contraction[J]. Biofactors,2021,1(11):56−66.
[18]	LEE J, LEE S, LEE M S, et al. The effects of high hydrostatic pressure extract of mulberry fruit on hepatic cholesterol metabolism in rats[J]. Current Developments in Nutrition, 2019, 3(Suppl 1): 6−13.
[19]	吴亚平. 黑米和桑葚花色苷对大鼠MPS的改善作用[D]. 天津: 天津科技大学, 2019. WU Y P. Ameliorative effect of black rice and mulberry anthocyanins on MPS in rats[D]. Tianjin: Tianjin University of Science and Technology, 2019.
[20]	董雯, 王蓉. 白藜芦醇诱导细胞自噬在神经退行性疾病进展中的作用[J]. 药学学报,2016,51(1):18−22. [DONG W, WANG R. The role of resveratrol-induced autophagy in the progression of neurodegenerative diseases[J]. Journal of Pharmacy,2016,51(1):18−22. DONG W, WANG R. The role of resveratrol-induced autophagy in the progression of neurodegenerative diseases[J]. Journal of Pharmacy, 2016, 51(1): 18-22.
[21]	杨婕. 桑葚粗提液对NP染毒大鼠神经行为毒性的解毒机制研究[D]. 广州: 华南农业大学, 2016. YANG J. Study on the detoxification mechanism of crude mulberry extract on neurobehavioral toxicity in rats exposed to NP[D]. Guangzhou: South China Agricultural University, 2016.
[22]	娄在祥, 王洪新, 吕文平, 等. 微波辅助提取牛蒡叶多酚及其抗氧化、抗菌活性研究[J]. 食品与发酵工业,2010,36(1):161−165. [LOU Z X, WANG H X, LU W P, et al. Microwave-assisted extraction of polyphenols from burdock leaves and their antioxidant and antibacterial activities[J]. Food and Fermentation Industry,2010,36(1):161−165. doi: 10.13995/j.cnki.11-1802/ts.2010.01.007 LOU Z X, WANG H X, LU W P, et al. Microwave-assisted extraction of polyphenols from burdock leaves and their antioxidant and antibacterial activities[J]. Food and Fermentation Industry, 2010, 36(1): 161-165. doi: 10.13995/j.cnki.11-1802/ts.2010.01.007
[23]	王凤娟, 孙飞龙, 叶文文, 等. pH示差法测定红菊苣中花青素条件的优化[J]. 包装与食品机械,2018,36(5):61−64. [WANG F J, SUN F L, YE W W, et al. Optimization of conditions for determination of anthocyanins in red chicory by pH differential method[J]. Packaging and Food Machinery,2018,36(5):61−64. doi: 10.3969/j.issn.1005-1295.2018.05.012 WANG F J, SUN F L, YE W W, et al. Optimization of conditions for determination of anthocyanins in red chicory by pH differential method[J]. Packaging and Food Machinery, 2018, 36(5): 61-64. doi: 10.3969/j.issn.1005-1295.2018.05.012
[24]	MA Z L, DU B, LI J, et al. An insight into anti-inflammatory activities and inflammation related diseases of anthocyanins: A review of both in vivo and in vitro investigations[J]. International Journal of Molecular Sciences,2021,22(20):11076. doi: 10.3390/ijms222011076
[25]	李胜男. 壬基酚暴露诱发大鼠焦虑样行为及对BDNF-TrkB信号网络的调控[D]. 遵义: 遵义医科大学, 2021. LI S N. Nonylphenol exposure induced anxiety-like behavior and regulation of BDNF-TrkB signaling network in rats[D]. Zunyi: Zunyi Medical University, 2021.
[26]	尹忞强, 申丽媛, 姜泽昆, 等. 白藜芦醇对免疫力低下小鼠的免疫调节作用研究[J]. 食品科技,2021,46(9):216−220. [YIN J Q, SHEN L Y, JIANG Z K, et al. Study on the immunomodulatory effect of resveratrol on immunocompromised mice[J]. Food Science and Technology,2021,46(9):216−220. doi: 10.13684/j.cnki.spkj.2021.09.035 YIN J Q, SHEN L Y, JIANG Z K, et al. Study on the immunomodulatory effect of resveratrol on immunocompromised mice[J]. Food Science and Technology, 2021, 46(9): 216-220. doi: 10.13684/j.cnki.spkj.2021.09.035
[27]	范奇元, 李卫华, 金泰廙, 等. 壬基酚在大鼠组织中的分布与清除[J]. 卫生毒理学杂志,2001,1(1):42−43. [FAN Q Y, LI W H, JIN T L, et al. Distribution and clearance of nonylphenol in rat tissues[J]. Journal of Health Toxicology,2001,1(1):42−43. FAN Q Y, LI W H, JIN T L, et al. Distribution and clearance of nonylphenol in rat tissues[J]. Journal of Health Toxicology, 2001, 1(1): 42-43.
[28]	GUO C, YANG L, WAN C X, et al. Anti-neuroinflammatory effect of sophoraflavanone G from Sophora alopecuroides in LPS-activated BV2 microglia by MAPK, JAK/STAT and Nrf2/HO-1 signaling pathways[J]. Phytomedicine,2016,23(13):1629−1637. doi: 10.1016/j.phymed.2016.10.007
[29]	LU J N, PANCHANATHAN R, LEE W S, et al. Anthocyanins from the fruit of Vitis coignetiae Pulliat inhibit TNF-augmented cancer proliferation, migration, and invasion in A549 cells[J]. Asian Pacific Journal of Cancer Prevention: APJCP,2017,18(11):2919−2923.
[30]	孙世光. 基于“行为组学”模式焦虑动物模型评价技术体系构建[D]. 济南: 山东中医药大学, 2016. SUN S G. Based on the ''behavior group'' model anxiety animal model evaluation technical system is constructed[D]. Jinan: Shandong University of traditional Chinese Medicine, 2016.
[31]	VALCHEVA-KUZMANOVA S, EFTIMOV M, BELCHEVA I, et al. Anti-anxiety effect of Aronia melanocarpa fruit juice administered subchronically to rats[J]. Farmacia,2016,64(3):367−371.
[32]	FERNANDEZ D R, RODRIGUEZ J F, GUZMAN R I, et al. Effect of blackberry juice (Rubus fruticosus L. ) on anxiety-like behaviour in Wistar rats[J]. International Journal of Food Sciences and Nutrition,2019,70(7):856−867. doi: 10.1080/09637486.2019.1580680
[33]	RAUF A, IMRAN M, BUTT M S, et al. Resveratrol as an anti-cancer agent: A review[J]. Critical Reviews in Food Science and Nutrition,2018,58(9):1428−1447. doi: 10.1080/10408398.2016.1263597
[34]	SCHANTZ S L, WIDHOLM J J. Cognitive effects of endocrine-disrupting chemicals in animals[J]. Environmental Health Perspectives,2001,109(12):1197−1206. doi: 10.1289/ehp.011091197
[35]	DI DALMAZI G, GIULIANI C. Plant constituents and thyroid: A revision of the main phytochemicals that interfere with thyroid function[J]. Food Chem Toxicol,2021,152:112158. doi: 10.1016/j.fct.2021.112158
[36]	王征. 游离甲状腺素与游离三碘甲状腺原氨酸的时间分辨荧光免疫检测试剂的研究[D]. 广州: 南方医科大学, 2013. WANG Z. Study on time-resolved fluorescence immunoassay of free thyroxine and free triiodothyronine[D]. Guangzhou: Southern Medical University, 2013.

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