Properties of<i> Pseudomonas</i> <i>aeruginosa</i> Phage K4 and Its Applications in Food Preservation

PANG Wenjing; HAN Qingzhu; YOU Jiajia; LI Donghang; LI Peize; LI Yueying; YANG Hongjiang

doi:10.13386/j.issn1002-0306.2021110193

Volume 43 Issue 16

Aug. 2022

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Science and Technology of Food Industry > 2022 > 43(16): 130-139. > DOI: 10.13386/j.issn1002-0306.2021110193

PANG Wenjing, HAN Qingzhu, YOU Jiajia, et al. Properties of Pseudomonas aeruginosa Phage K4 and Its Applications in Food Preservation[J]. Science and Technology of Food Industry, 2022, 43(16): 130−139. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110193.

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Properties of Pseudomonas aeruginosa Phage K4 and Its Applications in Food Preservation

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China

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Received Date: November 16, 2021
Available Online: June 14, 2022

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Abstract

Objective: To study phage applications in the control of bacteria contaminations, Pseudomonas aeruginosa phage K4 was characterized for its biological characteristics and inhibition effects of bacterial growth. Methods: One-step growth curve, phage stability, genome sequencing, comparative genomics analysis, and growth inhibition curve were conducted in this work. Results: One-step growth curve showed that phage K4 had a latent phase of 15 min and a burst size of about 85 PFU/infection center, displaying a strong infectivity. Under different MOIs (multiplicity of infection), phage K4 efficiently inhibited the growth of the host cells. Genomic analysis revealed that phage K4 had a genome of 50358 bp with 77 coding sequences and 1 tRNA-Arg gene. Comparative genomics showed that the genome of phage K4 shared an identity of 95.08% with Pseudomonas virus PA11 which belongs to the Paundecimvirus genus of the Zobellviridae family, suggesting that phage K4 might be a new member of the Paundecimvirus genus. In application assays, phage K4 could significantly control the growth of the host cells in sterilized milk and ready-to-eat beef samples along with obvious reproductions of phage K4. In conclusion, the data suggested that phage K4 was a lytic virus that replicated rapidly with relatively high bactericidal activity. Conclusion: The genome of phage K4 did not carry genes for integration, antibiotic resistance, and virulence, appropriately to be selected as a biocontrol agent in the control and prevention of P. aeruginosa contaminations in foods.
- Pseudomonas aeruginosa,
- phage,
- inhibitory activity,
- genome annotation,
- application

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