Immobilization of Cellulase by Chitosan-Coated Magnetic Nanoparticles

WANG Lin; LIU Rongxu; LIU Danyi; WANG Yucong; HAN Jianchun

doi:10.13386/j.issn1002-0306.2021110183

Volume 43 Issue 16

Aug. 2022

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Science and Technology of Food Industry > 2022 > 43(16): 74-80. > DOI: 10.13386/j.issn1002-0306.2021110183

WANG Lin, LIU Rongxu, LIU Danyi, et al. Immobilization of Cellulase by Chitosan-Coated Magnetic Nanoparticles[J]. Science and Technology of Food Industry, 2022, 43(16): 74−80. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110183.

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Immobilization of Cellulase by Chitosan-Coated Magnetic Nanoparticles

1.
College of Food Science, Northeast Agricultural University, Harbin 150030, China
2.
Hei Longjiang Green Food Research Institute, Harbin 150028, China

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Received Date: November 15, 2021
Available Online: June 12, 2022

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Abstract

Abstract

The design of enzyme-carrier complexes with high stability and selectivity is the focus of research in the field of immobilized enzymes. In this study, magnetic nanoparticles were prepared by precipitation using epichlorohydrin as surfactant and coated with chitosan to immobilize cellulase. Scanning electron microscope (SEM), vibrating sample magnetometer (VSM) and Fourier transform infrared spectrometer (FTIR infrared spectroscopy) were used to characterize Fe₃O₄ chitosan magnetic nanoparticles, and the characterization and enzymatic properties of immobilized cellulase were studied. Results showed that the prepared magnetic nanoparticles were crystallographically intact and the cellulase was effectively immobilized on the Fe₃O₄-chitosan carrier surface. In addition, immobilised cellulase had better acid-base stability and thermal stability than free cellulase. It had good activity in the range of pH2~9, and also with better thermostability, remained nearly 50% activity for 4 h at 60 and 70 ℃. After 10 cycles of recycling, the immobilized cellulase remained at a high activity of 52.6%, indicating that Fe₃O₄-chitosan could be used as an effective carrier for immobilized cellulase, providing a reference for further applications of the immobilized enzyme.
- magnetic nanoparticles,
- surface modification,
- chitosan,
- cellulase,
- immboilization

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References

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