Optimization of Deep Eutectic Solvent-Ultrasonic Assisted Extraction and Component Analysis of Polyphenols from Chestnut Shells

ZHANG Xiaoyun; MEI Xiaohong

doi:10.13386/j.issn1002-0306.2021110123

Volume 43 Issue 16

Aug. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(16): 230-237. > DOI: 10.13386/j.issn1002-0306.2021110123

ZHANG Xiaoyun, MEI Xiaohong. Optimization of Deep Eutectic Solvent-Ultrasonic Assisted Extraction and Component Analysis of Polyphenols from Chestnut Shells[J]. Science and Technology of Food Industry, 2022, 43(16): 230−237. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110123.

Citation:

PDF (3447 KB)

Optimization of Deep Eutectic Solvent-Ultrasonic Assisted Extraction and Component Analysis of Polyphenols from Chestnut Shells

ZHANG Xiaoyun^{1, 2,},
MEI Xiaohong^{1, 2, ,}

1.
College of Food Science and Nutrition Engineering, China Agricultural University, Beijing 100083, China
2.
Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), Ministry of Agriculture and Rural Affairs, Beijing 100083, China)

More Information

Received Date: November 11, 2021
Available Online: June 14, 2022

Graphical Abstract

Abstract

Abstract

In this study, deep eutectic solvents (DESs) coupled with ultrasound-assisted extraction (UAE) were applied to extract polyphenol from chestnut shells and the components were identified. Eight different DESs were evaluated as candidate solvents and fourier transform infrared spectroscopy (FTIR) was performed to confirm the formation of hydrogen bonds between hydrogen bond acceptor and hydrogen bond donor. Based on the single factor experiments, the response surface methodology (RSM) was applied, ultrasonic power, liquid-solid ratio and water content were further evaluated in order to optimize the total ployphenol yield. Besides, the extracts were recovered by macroporous resin for the identification of phenolic compounds. As a result, DES-1 (choline chloride:oxalic acid=1:1) exhibited a highest extraction yield. After that, DES-1-based UAE process was optimized and the optimal conditions were as follows: Ultrasonic power 348 W, liquid-solid ratio 42:1 mL/g and water content 32%. The extraction yield (99.66±2.63) mg/g of the model validation experiment was demonstrated to be in accordance with the predicted value 99.44 mg/g, which was significantly higher than that obtained by using traditional solvents (water and 40% ethanol). In addition, phenolic compounds was efficiently recovered from DES extracts with high recovery yield (97.92%±1.78%) by using AB-8 macroporous resin. 13 phenolic compounds were preliminarily identified from DES-1-based UAE extracts by UHPLC-Q-TOF-MS/MS. In conclusion, this study would provide a green and efficient method to extract polyphenols from chestnut shells, providing technical support for the development and utilization of chestnut shells waste.
- chestnut shells,
- polyphenol,
- deep eutectic solvents,
- extraction

FullText(HTML)

References (34)

References

[1]	PINTO D, VIEIRA E F, PEIXOTO A F, et al. Optimizing the extraction of phenolic antioxidants from chestnut shells by subcritical water extraction using response surface methodology[J]. Food Chemistry,2021,334:127521. doi: 10.1016/j.foodchem.2020.127521
[2]	de VASCONCELOS, BENNETT R N, QUIDEAU S, et al. Evaluating the potential of chestnut (Castanea sativa Mill. ) fruit pericarp and integument as a source of tocopherols, pigments and polyphenols[J]. Industrial Crops and Products,2010,31(2):301−311. doi: 10.1016/j.indcrop.2009.11.008
[3]	VELLA F M, LARATTA B, CARA F L, et al. Recovery of bioactive molecules from chestnut (Castanea sativa Mill.) by-products through extraction by different solvents[J]. Natural Product Research,2017,32(9):1022−1032.
[4]	MORANA A, SQUILLACI G, PAIXÃO S M, et al. Development of an energy biorefinery model for chestnut (Castanea sativa Mill.) shells[J]. Energies,2017,10(10):1504. doi: 10.3390/en10101504
[5]	王世伟, 张小慧, 刘霞, 等. 板栗壳质量标准研究[J]. 中国药物与临床,2015,15(7):945−947. [WANG S W, ZHANG X H, LIU X, et al. Study on quality standard of chestnut shell[J]. Chinese Remedies & Clinics,2015,15(7):945−947. WANG S W, ZHANG X H, LIU X, et al. Study on quality standard of chestnut shell[J]. Chinese Remedies & Clinics, 2015, 15(7): 945-947.
[6]	WU L F, LI L, CHEN S J, et al. Deep eutectic solvent-based ultrasonic-assisted extraction of phenolic compounds from Moringa oleifera L. leaves: Optimization, comparison and antioxidant activity[J]. Separation and Purification Technology,2020,247(15):117014.
[7]	SQUILLACI G, APONE F, SENA L M, et al. Chestnut (Castanea sativa Mill.) industrial wastes as a valued bioresource for the production of active ingredients[J]. Process Biochemistry,2018,64:228−236. doi: 10.1016/j.procbio.2017.09.017
[8]	WANG X H, WANG J P. Effective extraction with deep eutectic solvents and enrichment by macroporous adsorption resin of flavonoids from Carthamus tinctorius L[J]. Journal of Pharmaceutical and Biomedical Analysis,2019,176(30):112804.
[9]	BAJKACZ S, ADAMEK J. Evaluation of new natural deep eutectic solvents for the extraction of isoflavones from soy products[J]. Talanta,2017,168:329−335. doi: 10.1016/j.talanta.2017.02.065
[10]	CAO J, YANG, M, CAO F L, et al. Well-designed hydrophobic deep eutectic solvents as green and efficient media for the extraction of artemisinin from Artemisia annua leaves[J]. ACS Sustainable Chemistry & Engineering,2017,5(4):3270−3278.
[11]	DAS A K, SHARMA M, MONDAL D, et al. Deep eutectic solvents as efficient solvent system for the extraction of kappa-carrageenan from Kappaphycus alvarezii[J]. Carbohydrate Polymers,2016,136(20):930−935.
[12]	HERNÁNDEZ-CORROTO E, PLAZA M, MARINA M L, et al. Sustainable extraction of proteins and bioactive substances from pomegranate peel (Punica granatum L.) using pressurized liquids and deep eutectic solvents[J]. Innovative Food Science & Emerging Technologies,2020,60:102314.
[13]	JIANG Z M, WANG L J, GAO Z, et al. Green and efficient extraction of different types of bioactive alkaloids using deep eutectic solvents[J]. Microchemical Journal,2019,145:345−353. doi: 10.1016/j.microc.2018.10.057
[14]	WANG M, WANG J Q, ZHANG Y, et al. Fast environment-friendly ball mill-assisted deep eutectic solvent-based extraction of natural products[J]. Journal of Chromatography A,2016,1443(22):262−266.
[15]	YOO D E, JEONG K M, HAN S Y, et al. Deep eutectic solvent-based valorization of spent coffee grounds[J]. Food Chemistry,2018,255(30):357−364.
[16]	SUKOR N F, SELVAM V P, JUSOH R, et al. Intensified DES mediated ultrasound extraction of tannic acid from onion peel[J]. Journal of Food Engineering,2021,296:110437. doi: 10.1016/j.jfoodeng.2020.110437
[17]	OZTURK B, PARKINSON C, GONZALEZ-MIQUEL M, et al. Extraction of polyphenolic antioxidants from orange peel waste using deep eutectic solvents[J]. Separation and Purification Technology,2018,206(29):1−13.
[18]	WANG L, LUO Y, WU Y, et al. Fermentation and complex enzyme hydrolysis for improving the total soluble phenolic contents, flavonoid aglycones contents and bioactivities of guava leaves tea[J]. Food Chemistry,2018,264:189−198. doi: 10.1016/j.foodchem.2018.05.035
[19]	BUBALO C M, CURKO N, TOMASEVIC M, et al. Green extraction of grape skin phenolics by using deep eutectic solvents[J]. Food Chemistry,2016,200(1):159−166.
[20]	RAJHA H N, MHANNA T, EL KANTAR S, et al. Innovative process of polyphenol recovery from pomegranate peels by combining green deep eutectic solvents and a new infrared technology[J]. LWT-Food Science and Technology,2019,111:138−146. doi: 10.1016/j.lwt.2019.05.004
[21]	SAHA S K, DEY S, CHAKRABORTY R, et al. Effect of choline chloride-oxalic acid based deep eutectic solvent on the ultrasonic assisted extraction of polyphenols from Aegle marmelos[J]. Journal of Molecular Liquids,2019,287(1):110956.
[22]	MENG Z R, ZHAO J, DUAN H X, et al. Green and efficient extraction of four bioactive flavonoids from Pollen Typhae by ultrasound-assisted deep eutectic solvents extraction[J]. Journal of Pharmaceutical and Biomedical Analysis,2018,161(30):246−253.
[23]	ZHAO B Y, XU P, YANG F X, et al. Biocompatible deep eutectic solvents based on choline chloride: characterization and application to the extraction of rutin from Sophora japonica[J]. ACS Sustainable Chemistry & Engineering,2015,3(11):2746−2755.
[24]	MARIA V, JUSTYNA P W, VASIL A. The role of water in deep eutectic solvent-base extraction[J]. Journal of Molecular Liquids,2020,304:112747. doi: 10.1016/j.molliq.2020.112747
[25]	HUANG Y, FENG F, JIANG J, et al. Green and efficient extraction of rutin from tartary buckwheat hull by using natural deep eutectic solvents[J]. Food Chemistry,2016,221:1400−1405.
[26]	MANSUR A R, SONG N E, JANG H W, et al. Optimizing the ultrasound-assisted deep eutectic solvent extraction of flavonoids in common buckwheat sprouts[J]. Food Chemistry,2019,293:438−445. doi: 10.1016/j.foodchem.2019.05.003
[27]	YIN X S, ZHONG Z F, BIAN G L, et al. Ultra-rapid, enhanced and eco-friendly extraction of four main flavonoids from the seeds of Oroxylum indicum by deep eutectic solvents combined with tissue-smashing extraction[J]. Food Chemistry,2020,319:126555. doi: 10.1016/j.foodchem.2020.126555
[28]	FERNANDES A, FERNANDES I, CRUZ L, et al. Antioxidant and biological properties of bioactive phenolic compounds from Quercus suber L[J]. Journal of Agricultural & Food Chemistry,2009,57(23):11154−11160.
[29]	WANG Y, GAO Y, DING H, et al. Subcritical ethanol extraction of flavonoids from Moringa oleifera leaf and evaluation of antioxidant activity[J]. Food Chemistry,2017,218:152−158. doi: 10.1016/j.foodchem.2016.09.058
[30]	卢利平, 张利, 李铀, 等. 凤冈绿茶中原花青素的提取工艺优化[J]. 食品工业科技,2020,41(22):204−209,231. [LU L P, ZHANG L, LI Y, et al. Optimization of extraction technology of proanthocyanidins from green tea of Fenggang[J]. Science and Technology of Food Industry,2020,41(22):204−209,231. LU L P, ZHANG L, LI Y, et al. Optimization of extraction technology of proanthocyanidins from green tea of Fenggang[J]. Science and Technology of Food Industry, 2020, 41(22): 204-209, 231.
[31]	JIANG S, ZHAO X, LIU C, et al. Identification of phenolic compounds in fruits of Ribes stenocarpum Maxim. by UHPLC-QTOF/MS and their hypoglycemic effects in vitro and in vivo[J]. Food Chemistry,2020,344:128568.
[32]	OFOSU F K, ELAHI F, DALIRI B M, et al. UHPLC-ESI-QTOF-MS/MS characterization, antioxidant and antidiabetic properties of sorghum grains[J]. Food Chemistry,2020,337:127788.
[33]	ZHANG Y, OUYANG L, MAI X, et al. Use of UHPLC-QTOF-MS/MS with combination of in silico approach for distributions and metabolites profile of flavonoids after oral administration of Niuhuang Shangqing tablets in rats[J]. Journal of Chromatography B,2019,1114-1115:55−70. doi: 10.1016/j.jchromb.2019.03.021
[34]	AP A, CDS A, BRBA A, et al. Characterization of phenolic compounds by UHPLC-QTOF-MS/MS and functional properties of Syzygium malaccense leaves[J]. South African Journal of Botany,2021,139:418−426. doi: 10.1016/j.sajb.2021.01.036