Investigation of the Biogenic Amine Content in Jiangxi-made Sufu and Isolation and Identification of Amine-producing Bacteria

WANG Weiya; HUANG Ruiyu; LUO Yu; WU Shanshan; LIU Yuting; LI Jinlin

doi:10.13386/j.issn1002-0306.2021110004

Volume 43 Issue 12

Jun. 2022

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Science and Technology of Food Industry > 2022 > 43(12): 246-252. > DOI: 10.13386/j.issn1002-0306.2021110004

WANG Weiya, HUANG Ruiyu, LUO Yu, et al. Investigation of the Biogenic Amine Content in Jiangxi-made Sufu and Isolation and Identification of Amine-producing Bacteria[J]. Science and Technology of Food Industry, 2022, 43(12): 246−252. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110004.

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Investigation of the Biogenic Amine Content in Jiangxi-made Sufu and Isolation and Identification of Amine-producing Bacteria

1.
Nanchang Key Laboratory of Food Safety Detection and Controlling, Nanchang Inspection and Testing Center, Nanchang 330012, China
2.
College of Life Sciences, Jiangxi Normal Univercity, Nanchang 330022, China

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Received Date: November 01, 2021
Available Online: April 12, 2022

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Abstract

Abstract

The biogenic amine Level in Jiangxi-made sufu was analyzed by high performance liquid chromatography (HPLC) and amine-producing bacteria were isolated and identified by microbiological methods combined with HPLC confirmation. The results showed that total biogenic amine level ranged from 68.5 to 1084.0 mg/kg in sufu samples. Major biogenic amines were tryptamine, putrescine, histamine and tyramine, among which putrescine and tyramine were of the highest level. Biogenic amine level produced by 9 amine-producing bacteria ranged from 93.8 to 369.2 mg/L. For six bacteria with relatively high amine productivity, phenotype and genotype 16S rDNA sequence determination had found one Bacillus sporothermodurans strain, one Rummeliibacillus stabekisii strain, three Lysinacillus boronitolerans strains and one Staphylococcus piscifermentans strain. The current research offered theoretical evidence for edible safety evaluation of sufu and served as research basis to investigate how to inhibit activity of amine-producing bacteria in sufu.
- sufu,
- biogenic amine,
- amine-producing bacteria,
- isolation,
- identification

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References

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