Optimization of Conditions for γ-Aminobutyric Acid Yield by Co-fermentation of Enterococcus faecium with Saccharomyces cerevisiae and Mechanism Research

SUN Xiangyang; WANG Jie; YAO Hongmei; ZHENG Miaoxin; LI Chanyuan; ZHANG Shuming; ZHANG Qing

doi:10.13386/j.issn1002-0306.2021100274

Volume 43 Issue 15

Jul. 2022

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Science and Technology of Food Industry > 2022 > 43(15): 132-138. > DOI: 10.13386/j.issn1002-0306.2021100274

SUN Xiangyang, WANG Jie, YAO Hongmei, et al. Optimization of Conditions for γ-Aminobutyric Acid Yield by Co-fermentation of Enterococcus faecium with Saccharomyces cerevisiae and Mechanism Research[J]. Science and Technology of Food Industry, 2022, 43(15): 132−138. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100274.

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Optimization of Conditions for γ-Aminobutyric Acid Yield by Co-fermentation of Enterococcus faecium with Saccharomyces cerevisiae and Mechanism Research

College of Food and Bioengineering, Xihua University, Chengdu 610039, China

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Received Date: October 25, 2021
Available Online: May 29, 2022

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Abstract

Abstract

In order to improve the yield of γ-aminobutyric acid (GABA), the co-fermentation conditions of Enterococcus faecium AB157 and Saccharomyces cerevisiae SC-125 were optimized by one-factor-at-a-time method and response surface methodology (RSM). Simultaneously, the enzyme activity of glutamate decarboxylase (GAD) was analyzed under optimal conditions in co-fermentation and single strain fermentation systems, and the mechanism of high GABA yield was explored by adding cell-free supernatant (CFS). The optimization results showed that when the overall quantity of inoculum was 2% (V/V), the optimal co-fermentation conditions were as follows: The fermentation temperature was 35 ℃, the inoculum proportions of E. faecium AB157 and S. cerevisiae SC-125 was 5:1 (V/V), and the L-monosodium glutamate concentration was 12 g/L with shaking fermentation for 96 h. In addition, the yield of 6.55 g/L GABA was 1.78 times higher than in single strain fermentation systems. The GAD enzyme activity analysis showed that co-fermentation could significantly improve GAD enzyme activity. Meanwhile, GABA yield could be significantly increased by adding CFS of E. faecium AB157 or S. cerevisiae SC-125. This study served as a theoretical foundation for the discussion of the co-fermentation of E. faecium and S. cerevisiae to increase GABA yield and the mechanism of high GABA yield.
- Enterococcus faecium,
- Saccharomyces cerevisiae,
- co-fermentation,
- γ-aminobutyric acid,
- glutamate decarboxylase

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References

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