Structural Characterization of Lipu Taro Globulin and Its Glucose Metabolism Activity on HepG2 Cells

MA Erlan; ZHANG Fan; LÜ Chunqiu; TU Lian; WANG Weiliang; LIN Ying

doi:10.13386/j.issn1002-0306.2021100267

Volume 43 Issue 15

Jul. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(15): 359-365. > DOI: 10.13386/j.issn1002-0306.2021100267

MA Erlan, ZHANG Fan, LÜ Chunqiu, et al. Structural Characterization of Lipu Taro Globulin and Its Glucose Metabolism Activity on HepG2 Cells[J]. Science and Technology of Food Industry, 2022, 43(15): 359−365. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100267.

Citation:

PDF (2388 KB)

Structural Characterization of Lipu Taro Globulin and Its Glucose Metabolism Activity on HepG2 Cells

1.
College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China
2.
Nanning Customs Technology Center, Nanning 530021, China

More Information

Received Date: October 25, 2021
Available Online: May 26, 2022

Graphical Abstract

Abstract

Abstract

In order to characterize the basic properties of Lipu taro globulin and explore the mechanism of the effect on cell glucose metabolism globulin, thermal stability, amino acid composition and secondary structure composition were determined, and the effects of different concentrations of Lipu taro globulin on glucose consumption, liver glycogen content, hexokinase and pyruvate kinase activities of insulin resistant HepG2 cells were studied. The results showed that the denaturation temperature of Lipu taro globulin was 79.30±1.36 ℃, and the corresponding enthalpy values was 7.46±0.53 J/g. The globulin was rich of aspartic acid and glutamic acid, with an amino acid score of 103.10%. The secondary structure composition: The contents of β-sheet , random coil, β-turn and α-helix were 39.42%～42.14%, 26.34%～31.38%, 18.25%～24.16% and 5.04%～13.27%. Lipu taro globulin could affect glucose metabolism of insulin resistant cells, compared with the control group, Lipu taro globulin high dose group (500 μg/mL) increased glucose consumption by 40.62%±0.98%, increased liver glycogen content by 26.35%±1.13%, increased hexokinase activity by 54.63%±2.48% and increased pyruvate kinase activity by 44.29%±2.64%. In conclusion, Lipu taro globulin could promote cell glucose absorption, increase liver glycogen synthesis and improve the activities of hexokinase and pyruvate kinase, thus affecting glucose metabolism, the mechanism of action might be related to promoting insulin secretion and enhancing glycolysis.
- Lipu taro globulin,
- structural characterization,
- insulin resistance,
- HepG2 cells,
- glucose metabolism

FullText(HTML)

References (33)

References

[1]	杨月欣, 崔红梅, 王岩, 等. 常见谷类和薯类的血糖生成指数[J]. 营养学报,2003(2):185−189. [YANG Yuexin, CUI Hongmei, WANG Yan, et al. The glycemic index of common cereals and tubers products[J]. Journal of Nutrition,2003(2):185−189. YANG Yuexin, CUI Hongmei, WANG Yan, et al. The glycemic index of common cereals and tubers products[J]. 营养学报, 2003(2): 185-189.
[2]	WU C Y, LIN K W. The antioxidative characteristics of taro and sweet potato protein hydrolysates and their inhibitory capability on angiotensin converting enzyme[J]. Food Science and Technology Research,2017,23(6):845−853. doi: 10.3136/fstr.23.845
[3]	MOHAMMED A I, MEGAN J B, ALBERT W N, et al. Tuber storage proteins as potential precursors of bioactive peptides: An in silico analysis[J]. International Journal of Peptide Research and Therapeutics,2019,25(2):437−446. doi: 10.1007/s10989-018-9688-7
[4]	PEREIRA P R, WINTER H C, VERICIMO M A, et al. Structural analysis and binding properties of isoforms of tarin, the GNA-related lectin from Colocasia esculenta[J]. Biochimica et Biophysica Acta,2015,1854(1):20−30. doi: 10.1016/j.bbapap.2014.10.013
[5]	PEREIRA P R, AGUILA E M D, VERICIMO M A, et al. Purification and characterization of the lectin from taro (Colocasia esculenta) and its effect on mouse splenocyte proliferation in vitro and in vivo[J]. The Protein Journal,2014,33(1):92−99. doi: 10.1007/s10930-013-9541-y
[6]	PEREIRA P R, CORREA A C N T, VERICIMO M A, et al. Tarin, a potential immunomodulator and COX-inhibitor lectin found in taro (Colocasia esculenta)[J]. Comprehensive Reviews in Food Science and Food Safety,2018,17(4):878−891. doi: 10.1111/1541-4337.12358
[7]	KUNDU N, CAMPBELL P, HAMPTON B, et al. Antimetastatic activity isolated from Colocasia esculenta (taro)[J]. Anti-Cancer Drugs,2012,23(2):200−211. doi: 10.1097/CAD.0b013e32834b85e8
[8]	YU J, LIU P, DUAN J, et al. Itches-stimulating compounds from Colocasia esculenta (taro): Bioactive-guided screening and LC-MS/MS identification[J]. Bioorganic & Medicinal Chemistry Letters,2015,25(20):4382−4386.
[9]	CHAN Y S, WONG J H, NG T B. A cytokine-inducing hemagglutinin from small taros[J]. Protein & Peptide Letters,2010,17(7):823−830.
[10]	HIRAI M, NAKAMURA K, IMAI T, et al. cDNAs encoding for storage proteins in the tubers of taro (Colocasia esculenta Schott)[J]. The Japanese Journal of Genetics,1993,68(3):229−236. doi: 10.1266/jjg.68.229
[11]	BEZERRA I C, CASTRO L A B, NESHICH G, et al. A corm-specific gene encodes tarin, a major globulin of taro (Colocasia esculenta L. Schott)[J]. Plant Molecular Biology,1995,28(1):137−144. doi: 10.1007/BF00042045
[12]	KUMARI B, SHAMA P, NATH A K. α-Amylase inhibitor in local Himalyan collections of Colocasia: Isolation, purification, characterization and selectivity towards α-amylases from various sources[J]. Pesticide Biochemistry and Physiology,2012,103(1):49−55. doi: 10.1016/j.pestbp.2012.03.003
[13]	MCEWAN R, MADIVHA R P, DJAROVA T, et al. Alpha-amylase inhibitor of amadumbe (Colocasia esculenta): Isolation, purification and selectivity toward α-amylases from various sources[J]. African Journal of Biochemistry Research,2010,4(9):220−224.
[14]	SELTZER R D, STRUMEYER D H. Purification and characterization of esculentamin, a proteinaceous α-amylase inhibitor from the taro root, Colocasia esculenta[J] Journal of Food Biochemistry, 2010.14(3), 199-217.
[15]	吴嘉璠. 茶黄素在HepG2细胞和2型糖尿病小鼠模型中改善胰岛素抵抗及其分子机制研究[D]. 杭州: 浙江大学, 2020. WU Jiafan. Study on the improvement of insulin resistance and molecular mechanism of theafronin in HepG2 cells and mice model of type 2 diabetes mellitus[D]. Hangzhou: Zhejiang University, 2020.
[16]	方飞, 吴新荣, 罗明俐, 等. HepG2细胞胰岛素抵抗模型的建立及在筛选桑叶有效部位中的应用[J]. 医药导报,2012,31(6):691−694. [FANG Fei, WU Xinrong, LUO Mingli, et al. Establishment of insulin resistance model of HepG2 cells and its application in screening effective parts of mulberry leaves[J]. Medical Review,2012,31(6):691−694. doi: 10.3870/yydb.2012.06.001 FANG Fei, WU Xinrong, LUO Mingli, et al. Establishment of insulin resistance model of HepG2 cells and its application in screening effective parts of Mulberry leaves[J]. Medical review, 2012, 31(6): 691-694. doi: 10.3870/yydb.2012.06.001
[17]	HU S, FAN X, QI P, et al. Identification of anti-diabetes peptides from Spirulina platensis[J]. Journal of Functional Foods,2019,56:333−341. doi: 10.1016/j.jff.2019.03.024
[18]	马二兰, 林莹, 涂连, 等. 芋头球蛋白的提取纯化及其对α-淀粉酶和α-葡萄糖苷酶抑制活性研究[J]. 食品工业科技,2021,42(14):25−32. [MA Erlan, LIN Ying, TU Lian, et al. Extraction and purification of taro globulin and its inhibitory activity against α-amylase and α-glucosidase[J]. Science and Technology of Food Industry,2021,42(14):25−32. MA Erlan, LIN Ying, TU Lian, et al. Extraction and purification of taro globulin and its inhibitory activity against α-amylase and α-glucosidase[J]. Science and Technology of Food Industry, 201, 42(14): 25-32.
[19]	RAO Q, LABIZA T P. Effect of moisture content on selected physicochemical properties of two commercial hen egg white powders[J]. Food Chemistry,2012,132(1):373−384. doi: 10.1016/j.foodchem.2011.10.107
[20]	SHARMA G M, MUNDOMA C, SEAVY M, et al. Purification and biochemical characterization of Brazil nut (Bertholletia excelsa L.) seed storage proteins[J]. Journal of Agricultural & Food Chemistry,2010,58(9):5714−5723.
[21]	孙佳悦, 钱方, 姜淑娟, 等. 基于红外光谱分析热处理对牛乳蛋白质二级结构的影响[J]. 食品科学,2017,38(23):82−86. [SUN Jiayue, QIAN Fang, JIANG Shujuan, et al. Effects of heat treatment on protein secondary structure of milk based on infrared spectroscopy[J]. Food Science,2017,38(23):82−86. doi: 10.7506/spkx1002-6630-201723014 SUN Jiayue, QIAN Fang, JIANG Shujuan, et al. Effects of heat treatment on protein secondary structure of milk based on infrared spectroscopy[J]. Food Science, 2017, 38(23): 82-86. doi: 10.7506/spkx1002-6630-201723014
[22]	陈文, 王湘君, 赵阳, 等. 酸水解-全自动氨基酸分析仪测定方格星虫中氨基酸[J]. 食品工业科技,2017,38(3):299−304. [CHEN Wen, WANG Xiangjun, ZHAO Yang, et al. Determination of amino acids from Sipunculs nudus by acid hydrolysis-automatic amino acid analyzer[J]. Science and Technology of Food Industry,2017,38(3):299−304. CHEN Wen, WANG Xiangjun, ZHAO Yang, et al. Determination of amino acids from Sipunculs nudus by acid hydrolysis-automatic amino acid analyzer[J]. Science and Technology of Food Industry, 2017, 38(3): 299-304.
[23]	尤玲玲, 陈永慧, 刘金福, 等. 苦瓜皂苷对胰岛素抵抗HepG2细胞葡萄糖消耗量的影响[J]. 食品工业科技,2014,35(5):338−340. [YOU Lingling, CHEN Yonghui, LIU Jinfu, et al. The effect of momordica charantia saponins on glucose consumption of insulin-resistant HepG2 cells[J]. Science and Technology of Food Industry,2014,35(5):338−340. YOU Lingling, CHEN Yonghui, LIU Jinfu, et al. The effect of momordica charantia saponins on glucose consumption of insulin-resistant HepG2 cells[J]. Science and Technology of Food Industry, 2014, 35(5): 338-340.
[24]	童晶晶. 香芋蛋白的提取及其胰蛋白酶抑制剂的研究[D]. 广州: 华南理工大学, 2016. TONG Jingjing. Study on extraction of taro protein and its trypsin inhibitor[D]. Guangzhou: South China University of Technology, 2016.
[25]	刘志彤, 郑淋, 王晨阳, 等. 海参二肽基肽酶IV抑制肽的酶解制备及结构鉴定[J]. 现代食品科技,2020,36(8):166−174. [LIU Zhitong, ZHENG Lin, WANG Chenyang, et al. Preparation and structure identification of dipeptidyl peptidase IV inhibitory peptide from sea cucumber[J]. Modern Food Science and Technology,2020,36(8):166−174. LIU Zhitong, ZHENG Lin, WANG Chenyang, et al. Preparation and structure identification of dipeptidyl peptidase IV inhibitory peptide from sea cucumber[J]. Modern Food Science and Technology, 2020, 36(8): 166-174.
[26]	YU Z, YIN Y, ZHAO W, et al. Anti-diabetic activity peptides from albumin against α-glucosidase and α-amylase[J]. Food Chemistry,2012,135(3):2078−2085. doi: 10.1016/j.foodchem.2012.06.088
[27]	AUWAL I M, BESTER M J, NEITZ A W, et al. Rational in silico design of novel α-glucosidase inhibitory peptides and in vitro evaluation of promising candidates[J]. Biomedicine & Pharmacotherapy,2018,107:234−242.
[28]	MEISEL H. Multifunctional peptides encrypted in milk proteins[J]. BioFactors,2004,21(1-4):55−61. doi: 10.1002/biof.552210111
[29]	MOLLICA A, ZENGIN G, DURDAGI S, et al. Combinatorial peptide library screening for discovery of diverse α-glucosidase inhibitors using molecular dynamics simulations and binary QSAR models[J]. Journal of Biomolecular Structure & Dynamics,2018,1:726−740.
[30]	RAMADHAN, NAWAS, ZHANG, et al. Purification and identification of a novel antidiabetic peptide from Chinese giant salamander (Andrias davidianus) protein hydrolysate against α-amylase and α-glucosidase[J]. International Journal of Food Properties,2018,20(sup3):3360−3372.
[31]	MARELLA S, MADDIRELA D R, KUMAR E, et al. Mcy protein, a potential antidiabetic agent: Evaluation of carbohydrate metabolic enzymes and antioxidant status[J]. International Journal of Biological Macromolecules,2016,48:481−488.
[32]	JIANG N, ZHANG S, ZHU J, et al. Hypoglycemic, hypolipidemic and antioxidant effects of peptides from red deer antlers in streptozotocin-induced diabetic mice[J]. Tohoku Journal of Experimental Medicine,2015,236(1):71−79. doi: 10.1620/tjem.236.71
[33]	CHEN M D, PAN D D, ZHOU T Q, et al. Novel umami peptide IPIPATKT with dual dipeptidyl peptidase-IV and angiotensin I-converting enzyme inhibitory activities[J]. Journal of Agricultural and Food Chemistry,2021,69(19):5463−5470. doi: 10.1021/acs.jafc.0c07138

Supplements (0)

Cited By

Cited by

Periodical cited type(4)

1.	杨裴. 不同运动方式对人体DNA损伤、DNA甲基化和端粒长度的影响. 中国组织工程研究. 2024(01): 147-152 .
2.	邬丹，李惠霖，陈肖阳，程相雷，朱邦栋，吴浪，沈幸，柴仲平，曾茂茂. 烤馕中晚期糖基化终末产物和4-甲基咪唑的生成规律研究进展. 食品安全质量检测学报. 2024(09): 84-92 .
3.	李熙红，康丽丽，高春梅，董莉莉，王慧，魏清琳. 敦煌脐密功联合西药对改善IBS-D胃肠道症状疗效的评价. 中国民族民间医药. 2023(16): 108-111 .
4.	周荣华，陈一斌. 腹泻型肠易激综合征的中西医治疗研究进展. 中国医药导刊. 2022(11): 1119-1122 .