Optimization of Extraction Process of Flavonoids from Water Chestnut Shell and Effect on Anti-tumor Cell Proliferation Activity

LEI Qiuqi; YE Shijie; HUANG Yongkang; YANG Guo; ZHOU Min; WANG Hongxun; WANG Limei

doi:10.13386/j.issn1002-0306.2021100178

Volume 43 Issue 14

Jul. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(14): 224-232. > DOI: 10.13386/j.issn1002-0306.2021100178

LEI Qiuqi, YE Shijie, HUANG Yongkang, et al. Optimization of Extraction Process of Flavonoids from Water Chestnut Shell and Effect on Anti-tumor Cell Proliferation Activity[J]. Science and Technology of Food Industry, 2022, 43(14): 224−232. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100178.

Citation:

PDF (4465 KB)

Optimization of Extraction Process of Flavonoids from Water Chestnut Shell and Effect on Anti-tumor Cell Proliferation Activity

LEI Qiuqi¹,
YE Shijie²,
HUANG Yongkang²,
YANG Guo²,
ZHOU Min^{1, 3},
WANG Hongxun^{2, 3},
WANG Limei^{2, 3, ,}

1.
College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
2.
College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China
3.
Hubei Engineering Research Center for Fresh Food, Wuhan 430023, China

More Information

Received Date: October 18, 2021
Available Online: May 08, 2022

Graphical Abstract

Abstract

Abstract

This experiment was to optimize the extraction process of water chestnut shell flavonoids and investigate its inhibitory effect on the proliferation of human cervical cancer Hela cells. A single factor experiment was used to investigate the effects of extraction time, material-to-liquid ratio, ethanol concentration and extraction temperature on the yield of total flavonoids in water chestnut shells, and response surface analysis was carried out based on the yield of total flavonoids in water chestnut shells to obtain the extraction of water chestnut shell flavonoids. At the same time, the CCK-8 method was used to determine the effect of water chestnut flavonoids on the proliferation of human cervical cancer Hela cells, and to determine its IC₅₀ value to investigate the anti-tumor activity of water chestnut flavonoids. The experimental results showed that the best extraction process of water chestnut shell flavonoids was: extraction time 28 min, material-to-liquid ratio 1:33 g/mL, and ethanol concentration 53%. Under these conditions, the extraction rate of total flavonoids in the crude extract of water chestnut shell could reach 3.455%±0.16%. Incubation with the crude extract or purified solution of water chestnut shell flavonoids at concentrations of 5, 100, 200, 400, 800 µg/mL, all had a proliferation inhibitory effect on Hela cells, and there was a time and concentration dependence. Taking action time 48 h as an example, the IC₅₀ values of the crude extract of water chestnut shell flavonoids, the purified material of water chestnut shell flavonoids, and 5-Fu were 271.46, 268.16, and 152.09 µg/mL, respectively. Therefore, at the same time, the inhibitory effect of water chestnut extract on the proliferation of Hela cells was weaker than that of the positive control 5-Fu.
- water chestnut shell flavonoids,
- process optimization,
- human cervical cancer Hela cells,
- anti-tumor activity

FullText(HTML)

References (43)

References

[1]	周光雄, 吴志敏, 杨政红, 等. 菱角皮中鞣质类成分研究[J]. 时珍国医国药,2010,21(6):1414−1415. [ZHOU G X, WU Z M, YANG Z H, et al. Study on tannins in water chestnut skin[J]. Lishizhen Medicine and Materia Medica,2010,21(6):1414−1415. doi: 10.3969/j.issn.1008-0805.2010.06.054 ZHOU G X, WU Z M, YANG Z H, et al. Study on tannins in water chestnut skin[J]. Lishizhen Medicine and Materia Medica, 2010, 21 (6): 1414-1415. doi: 10.3969/j.issn.1008-0805.2010.06.054
[2]	江燕. 菱角壳化学成分及体外生物活性研究[D]. 杭州: 浙江工业大学, 2019. JIANG Y. Study on the chemical composition and in vitro biological activity of water chestnut shell[D]. Hangzhou: Zhejiang University of Technology, 2019.
[3]	左袁袁, 吕寒, 简暾昱, 等. 菱角壳化学成分及其药理作用研究进展[J]. 辽宁中医药大学学报,2019,21(4):94−99. [ZUO Y Y, LÜ H, JIAN T Y, et al. Research progress on the chemical constituents and pharmacological effects of water chestnut shells[J]. Journal of Liaoning University of Traditional Chinese Medicine,2019,21(4):94−99. ZUO Y Y, LÜ H, JIAN T Y, et al. Research progress on the chemical constituents and pharmacological effects of water chestnut shells[J]. Journal of Liaoning University of Traditional Chinese Medicine, 2019, 21(4): 94-99.
[4]	牛凤兰, 陈林, 宋德锋, 等. 菱角的化学成分及药效活性研究进展[J]. 中药材,2009,32(12):1926−1929. [NIU F L, CHEN L, SONG D F, et al. Research progress on the chemical composition and pharmacodynamic activity of water chestnut[J]. Chinese Medicinal Materials,2009,32(12):1926−1929. doi: 10.3321/j.issn:1001-4454.2009.12.044 NIU F L, CHEN L, SONG D F, et al. Research progress on the chemical composition and pharmacodynamic activity of water chestnut[J]. Chinese Medicinal Materials, 2009, 32(12): 1926-1929. doi: 10.3321/j.issn:1001-4454.2009.12.044
[5]	董晶莱, 高广春. 菱角壳的化学成分和药理活性研究进展[J]. 嘉兴学院医学院学报,2009,8(10):23−27. [DONG J L, GAO G C. Research progress on the chemical constituents and pharmacological activities of water chestnut shells[J]. Journal of Jiaxing University Medical College,2009,8(10):23−27. DONG J L, GAO G C. Research progress on the chemical constituents and pharmacological activities of water chestnut shells[J]. Journal of Jiaxing University Medical College, 2009, 8(10): 23-27.
[6]	任青. 菱角壳提取物的抗癌研究及其胃漂浮片的研制[D]. 天津: 天津大学, 2010. REN Q. Anti-cancer research of water chestnut shell extract and development of gastric floating tablets [D]. Tianjin: Tianjin University, 2010.
[7]	WU C H, DING Y Z. Study of different extracts from water caltrop shells on inhibiting lung cancer cell line A549[J]. Journal of TCM Univ of Hunan, 2012, 32(1): 27−30.
[8]	林秋生. 菱壳生物活性成分分析及抗胃癌机制研究[D]. 杭州: 浙江大学, 2013. LIN Q S. Analysis of bioactive components of rhizome and study on anti-gastric cancer mechanism[D]. Hangzhou: Zhejiang University, 2013.
[9]	NING Y, YU H N, ZHOU X M, et al. Optimal process for semi-bionic extraction of bioactive compounds against gastric cancer from water chestnut shell by uniform designation[J]. Journal of Zhejiang University (Agric. & Life Sci. ),2011,37(3):332−337.
[10]	DONG J L, GAO G C. Review on the phytochemical and pharmacological study of trapa shell[J]. Journal of Jiaxing University,2014,26(26):68−71.
[11]	牛凤兰, 李晨旭, 董威严, 等. 菱壳水提物对胃癌细胞抑制作用的实验研究[J]. 白求恩医科大学学报, 2001, 27(5): 495−496. NIU F L, LI C X, DONG W Y, et al. Experimental study on the inhibitory effect of water extract of water chestnut shells on gastric cancer cells[J]. Journal of Baiqiuen Medical University, 2001, 27(5): 495−496.
[12]	李亚军, 梁忠厚. 黄酮类化合物提取研究进展[J]. 粮食与油脂,2021,34(11):14−17. [LI Y J, LIANG Z H. Research progress on the extraction of flavonoids[J]. Food and Oils,2021,34(11):14−17. doi: 10.3969/j.issn.1008-9578.2021.11.004 LI Y J, LIANG Z H. Research progress on the extraction of flavonoids[J]. Food and Oils, 2021, 34(11): 14-17. doi: 10.3969/j.issn.1008-9578.2021.11.004
[13]	刘冬敏, 王建辉, 刘永乐. 菱角壳中黄酮、总酚和皂苷的提取及其抗氧化性研究[J]. 食品安全质量检测学报,2016,7(9):3668−3672. [LIU D M, WANG J H, LIU Y L. Extraction of flavonoids, total phenols and saponins from water chestnut shells and their antioxidant properties[J]. Journal of Food Safety and Quality Inspection,2016,7(9):3668−3672. LIU D M, WANG J H, LIU Y L. Extraction of flavonoids, total phenols and saponins from water chestnut shells and their antioxidant properties[J]. Journal of Food Safety and Quality Inspection, 2016, 7(9): 3668-367.
[14]	TULYAHAN V, BOONDEE K, MAHAWANICH T. Characteristics of starch from water chestnut (Trapa bispinosa Roxb.)[J]. Journal of Food Biochemistry,2005,29(4):337−348. doi: 10.1111/j.1745-4514.2005.00010.x
[15]	李丽, 李杰民, 盛金凤, 等. 超声波提取罗汉果渣中的黄酮优化工艺研究[J]. 食品研究与开发,2016,37(15):61−65. [LI L, LI J M, SHENG J F, et al. Study on the optimization technology of ultrasonic extraction of flavonoids from Luo Han Guo pomace[J]. Food Research and Development,2016,37(15):61−65. doi: 10.3969/j.issn.1005-6521.2016.15.015 LI L, LI J M, SHENG J F, et al. Study on the optimization technology of ultrasonic extraction of flavonoids from Luo Han Guo pomace[J]. Food Research and Development, 2016, 37 (15): 61-65 doi: 10.3969/j.issn.1005-6521.2016.15.015
[16]	胡金颖, 代立霞, 张丹参, 等. 酸枣仁中黄酮类物质药理作用研究进展[J]. 中国药理学与毒理学杂志,2021,35(10):785−786. [HU J Y, DAI L X, ZHANG D S, et al. Research progress on the pharmacological effects of flavonoids in jujube seed[J]. Chinese Journal of Pharmacology and Toxicology,2021,35(10):785−786. doi: 10.3867/j.issn.1000-3002.2021.10.115 HU J Y, DAI L X, ZHANG D S, et al. Research progress on the pharmacological effects of flavonoids in jujube seed[J]. Chinese Journal of Pharmacology and Toxicology, 2021, 35(10): 785-786. doi: 10.3867/j.issn.1000-3002.2021.10.115
[17]	李志平, 李护护. 银杏叶中黄酮类化合物的提取和分离[J]. 中国油脂,2021,46(2):131−134. [LI Z P, LI H H. Extraction and separation of flavonoids from ginkgo biloba leaves[J]. China Oils and Fats,2021,46(2):131−134. LI Z P, LI H H. Extraction and separation of flavonoids from ginkgo biloba leaves [J]. China Oils and Fats, 2021, 46(2): 131-134.
[18]	陈惠卿, 陈惠婷, 张颖, 等. 乙醇法制备葛花醇提物的工艺优化及其抗氧化活性[J]. 福建轻纺,2021(10):2−8,11. [CHEN H Q, CHEN H T, ZHANG Y, et al. Process optimization and antioxidant activity of ethanol extracts of Pueraria lobata flower prepared by ethanol[J]. Fujian Textile,2021(10):2−8,11. doi: 10.3969/j.issn.1007-550X.2021.10.001 CHEN H Q, CHEN H T, ZHANG Y, et al. Process optimization and antioxidant activity of ethanol extracts of Pueraria lobata flower prepared by ethanol[J]. Fujian Textile, 2021(10): 2- 8, 11. doi: 10.3969/j.issn.1007-550X.2021.10.001
[19]	李鹏婧, 柳旭光, 龙海荣, 等. 超声波辅助提取菱角壳总黄酮及抗氧化性研究[J]. 食品科技,2011,36(1):167−171. [LI P J, LIU X G, LONG H R, et al. Ultrasonic-assisted extraction of total flavonoids from water chestnut shells and their antioxidant properties[J]. Food Science and Technology,2011,36(1):167−171. LI P J, LIU X G, LONG H R, et al. Ultrasonic-assisted extraction of total flavonoids from water chestnut shells and their antioxidant properties [J]. Food Science and Technology, 2011, 36(1): 167-171.
[20]	高慧, 付强, 孙洁, 等. 黄酮类化合物及其纳米制剂在肿瘤防治中的研究进展[J]. 肿瘤代谢与营养电子杂志,2020,7(3):362−365. [GAO H, FU Q, SUN J, et al. Research progress of flavonoids and their nano-preparations in tumor prevention and treatment[J]. Electronic Journal of Tumor Metabolism and Nutrition,2020,7(3):362−365. GAO H, FU Q, SUN J, et al. Research progress of flavonoids and their nano-preparations in tumor prevention and treatment[J]. Electronic Journal of Tumor Metabolism and Nutrition, 2020, 7(3) : 362-365.
[21]	LISKOVA A, SAMEC M, KOKLESOVA L, et al. Flavonoids as an effective sensitizer for anti-cancer therapy: Insights into multi-faceted mechanisms and applicability towards individualized patient profiles[J]. The EPMA Journal,2021(prepublish):21−22.
[22]	WANG J G, JIAN W J, LI Y, et al. Nobiletin promotes the pyroptosis of breast cancer via regulation of miR-200b/JAZF1 axis[J]. The Kaohsiung Journal of Medical Sciences,2021,37(7):572−582. doi: 10.1002/kjm2.12371
[23]	GANAI S A, SHEIKH F A, BABA Z B, et al. Anticancer activity of the plant flavonoid luteolin against preclinical models of various cancers and insights on different signalling mechanisms modulated[J]. Phytotherapy Research: PTR,2021,35(7):3509−3532. doi: 10.1002/ptr.7044
[24]	王雪, 刘景楠, 夏顺利, 等. 中药总黄酮抗肿瘤活性及机制的研究现状[J]. 中国临床药理学杂志,2021,37(4):484−486,496. [WANG X, LIU J N, XIA S L, et al. Research status of anti-tumor activity and mechanism of total flavonoids of traditional Chinese medicine[J]. Chinese Journal of Clinical Pharmacology,2021,37(4):484−486,496. WANG X, LIU J N, XIA S L, et al. Research status of anti-tumor activity and mechanism of total flavonoids of traditional Chinese medicine[J]. Chinese Journal of Clinical Pharmacology, 2021, 37(4): 484-486, 496.
[25]	许地元, 叶萍, 李海丽, 等. 苞叶雪莲总黄酮提取工艺优化及其粗提物抗肿瘤活性[J]. 江苏农业科学,2021,49(4):130−134. [XU D Y, YE P, LI H L, et al. Optimization of the extraction process of the total flavonoids of Saussurea praecox and the anti-tumor activity of the crude extract[J]. Jiangsu Agricultural Sciences,2021,49(4):130−134. XU D Y, YE P, LI H L, et al. Optimization of the extraction process of the total flavonoids of Saussurea praecox and the anti-tumor activity of the crude extract [J]. Jiangsu Agricultural Sciences, 2021, 49(4): 130-134.
[26]	刘冬敏, 王建辉, 刘永乐, 等. 菱角壳中总黄酮提取工艺的优化[J]. 食品与机械,2016,32(5):173−176. [LIU D G, WANG J H, LIU Y L, et al. Optimization of extraction process of total flavonoids in water chestnut shells[J]. Food and Machinery,2016,32(5):173−176. LIU D G, WANG J H, LIU Y L, et al. Optimization of extraction process of total flavonoids in water chestnut shells[J]. Food and Machinery, 2016, 32(5): 173-176.
[27]	LI C B, XU M Y, CHEN Y, et al. Extraction of flavonoids from sword flower and methodology of its preparations[J]. International Journal of Food Science and Biotechnology, 2021, 6(1).
[28]	王艳, 张铁军. 微波萃取技术在中药有效成分提取中的应用[J]. 中草药,2005,36(3):67. [WANG Y, ZHANG T J. Application of microwave extraction technology in the extraction of effective components of traditional Chinese medicine[J]. Chinese Herbal Medicine,2005,36(3):67. WANG Y, ZHANG T J. Application of microwave extraction technology in the extraction of effective components of traditional Chinese medicine[J]. Chinese Herbal Medicine, 2005, 36(3): 67.
[29]	LIAO J Q, GUO Z R, YU G C. Process intensification and kinetic studies of ultrasound-assisted extraction of flavonoids from peanut shells[J]. Ultrasonics Sonochemistry,2021,76:56−61.
[30]	许一平, 王新功, 梁伟玲, 等. 酶解-超声偶联法提取桔梗茎叶总黄酮的工艺研究[J]. 化工管理,2020(29):19−20,34. [XU Y P, WANG X G, LIANG W L, et al. Study on the technology of extracting total flavonoids from Platycodon grandiflorum stems and leaves by enzymolysis-ultrasonic coupling[J]. Chemical Management,2020(29):19−20,34. doi: 10.3969/j.issn.1008-4800.2020.29.010 XU Y P, WANG X G, LIANG W L, et al. Study on the technology of extracting total flavonoids from Platycodon grandiflorum stems and leaves by enzymolysis-ultrasonic coupling[J]. Chemical Management, 2020(29): 19-20, 34. doi: 10.3969/j.issn.1008-4800.2020.29.010
[31]	郑菲艳, 洪佳敏, 徐晓俞, 等. 木豆叶提取液膜分离纯化研究[J]. 福建农业学报,2016,31(10):1122−1125. [ZHENG F Y, HONG J M, XU X Y, et al. Study on separation and purification of pigeon pea leaf extract with liquid membrane[J]. Fujian Journal of Agriculture,2016,31(10):1122−1125. ZHENG F Y, HONG J M, XU X Y, et al. Study on separation and purification of pigeon pea leaf extract with liquid membrane[J]. Fujian Journal of Agriculture, 2016, 31(10): 1122-1125.
[32]	牛凤兰, 黄占有, 吴秀华, 等. 菱角挥发成分超临界萃取及GC-MS方法检测[J]. 中国公共卫生,2009,25(1):119−120. [NIU F L, HUANG Z Y, WU X H, et al. Supercritical extraction and GC-MS detection of volatile components of water chestnut[J]. Chinese Public Health,2009,25(1):119−120. doi: 10.3321/j.issn:1001-0580.2009.01.061 NIU F L, HUANG Z Y, WU X H, et al. Supercritical extraction and GC-MS detection of volatile components of water chestnut [J]. Chinese Public Health, 2009, 25(1): 119-120. doi: 10.3321/j.issn:1001-0580.2009.01.061
[33]	SHIREEN A, FERDUSEE A M, MOUTIUR R M, et al. Computational and experimental studies to optimize the extraction of flavonoids from mango powder using response surface methodology[J]. Journal of Food Measurement and Characterization,2021(prepublish):1−12.
[34]	刘瑞芳, 张志迪, 陈梦茜, 等. PHF19对宫颈癌细胞增殖、侵袭和迁移的影响[J]. 牡丹江医学院学报,2021,42(3):8−12. [LIU R F, ZHANG Z D, CHEN M X, et al. The effect of PHF19 on the proliferation, invasion and migration of cervical cancer cells[J]. Journal of Mudanjiang Medical College,2021,42(3):8−12. LIU R F, ZHANG Z D, CHEN M X, et al. The effect of PHF19 on the proliferation, invasion and migration of cervical cancer cells[J]. Journal of Mudanjiang Medical College, 2021, 42(3): 8-12.
[35]	邓春玲, 朱坤, 刘涛瑞, 等. 敲低葡萄糖-6-磷酸酶催化亚基(G6PC)阻断AKT/mTOR通路抑制宫颈癌HeLa细胞增殖、侵袭和迁移[J]. 细胞与分子免疫学杂志,2021,37(6):520−526. [DENG C L, ZHU K, LIU T R, et al. Knockdown of the glucose-6-phosphatase catalytic subunit (G6PC) blocks the AKT/mTOR pathway and inhibits the proliferation, invasion and migration of cervical cancer HeLa cells[J]. Cells and Journal of Molecular Immunology,2021,37(6):520−526. DENG C L, ZHU K, LIU T R, et al. Knockdown of the glucose-6-phosphatase catalytic subunit (G6PC) blocks the AKT/mTOR pathway and inhibits the proliferation, invasion and migration of cervical cancer HeLa cells[J]. Cells and Journal of Molecular Immunology, 2021, 37(6): 520-526.
[36]	TOMINAGA H, ISHIYAMA M, OHSETO F, et al. A water-soluble tetrazolium salt useful for colorimetric cell viability assay[J]. Anal Commun,1999,36(1):47−50.
[37]	LIU A Q, XIA L. Comparative study on the optimal experiment conditions between CCK-8 and MTT in rabbit fibroblasts[J]. Medical Innovation of China,2013,10(2):12−13.
[38]	李玉霞, 尚庆坤, 许蕾蕾. AB-8大孔树脂对菱角壳黄酮提取物的吸附性能研究[J]. 北华大学学报(自然科学版),2006(5):403−406. [LI Y X, SHANG Q K, XU L L. Study on the adsorption performance of AB-8 macroporous resin to the extract of water chestnut shell flavonoids[J]. Journal of Beihua University (Natural Science Edition),2006(5):403−406. LI Y X, SHANG Q K, XU L L. Study on the adsorption performance of AB-8 macroporous resin to the extract of water chestnut shell flavonoids[J]. Journal of Beihua University (Natural Science Edition), 2006(5): 403- 406.
[39]	徐晶, 陈再兴, 袁昌鲁. 大孔吸附树脂富集纯化黄芩总黄酮的工艺研究[J]. 中医药学刊,2006(9):1648−1649. [XU J, CHEN Z X, YUAN C L. Study on the process of enrichment and purification of total flavonoids from Scutellaria baicalensis Georgi with macroporous adsorption resin[J]. Journal of Traditional Chinese Medicine,2006(9):1648−1649. doi: 10.3969/j.issn.1673-7717.2006.09.029 XU J, CHEN Z X, YUAN C L. Study on the process of enrichment and purification of total flavonoids from Scutellaria baicalensis Georgi with macroporous adsorption resin[J]. Journal of Traditional Chinese Medicine, 2006(9): 1648-1649. doi: 10.3969/j.issn.1673-7717.2006.09.029
[40]	李新明, 李群. 大枣黄酮的抗菌与抗肿瘤活性研究[J]. 农产品加工,2020(22):12−14. [LI X M, LI Q. Research on antibacterial and antitumor activities of jujube flavonoids[J]. Agricultural Products Processing,2020(22):12−14. LI X M, LI Q. Research on antibacterial and antitumor activities of jujube flavonoids[J]. Agricultural Products Processing, 2020(22): 12-14.
[41]	李亚男. 黄芪抗肿瘤的有效成分及作用机制的研究进展[D]. 重庆: 重庆医科大学, 2017. LI Y N. Research progress on anti-tumor active ingredients and mechanism of astragalus [D]. Chongqing: Chongqing Medical University, 2017.
[42]	袁燕, 李艳红, 段先乐, 等. 蒜头果蛋白对HeLa细胞体外抗肿瘤活性及稳定性研究[J]. 云南民族大学学报(自然科学版),2021,30(5):454−458. [YUAN Y, LI Y H, DUAN X L, et al. In vitro anti-tumor activity and stability of Allictocarpus protein on HeLa cells[J]. Journal of Yunnan Nationalities University (Natural Science Edition),2021,30(5):454−458. YUAN Y, LI Y H, DUAN X L, et al. In vitro anti-tumor activity and stability of Allictocarpus protein on HeLa cells[J]. Journal of Yunnan Nationalities University (Natural Science Edition), 2021, 30(5): 454-458 .
[43]	王姗姗, 刘明, 刘英, 等. MTT法测定多柔比星和氟尿嘧啶及顺铂对肝癌耐药细胞Bel-7402/ADM的IC₅₀值[J]. 医药报,2010,29(5):579−581. [WANG S S, LIU M, LIU Y, et al. MTT method to determine the IC₅₀ value of doxorubicin, fluorouracil and cisplatin on the resistance of liver cancer cell Bel-7402/ADM[J]. Medical Journal,2010,29(5):579−581. WANG S S, LIU M, LIU Y, et al. MTT method to determine the IC₅₀ value of doxorubicin, fluorouracil and cisplatin on the resistance of liver cancer cell Bel-7402/ADM [J]. Medical Journal, 2010, 29(5): 579-581.

[1]	CHEN Yang, WANG Peng, PAN Kaijin, WANG Zhe, XU Jian, ZHOU Junqiang, LIAO Ziwei. Optimization of the Extraction Process of Highland Barley β-glucan by Three-phase Partitioning and Its Molecular Weight Distribution[J]. Science and Technology of Food Industry, 2023, 44(14): 220-228. DOI: 10.13386/j.issn1002-0306.2022100064
[2]	ZHANG Lixia, SUN Xiaojing, WEI Songli, JIN Lu, MA Lin, SUN Qiang, LU Xin, ZHAO Mouming. Optimization of Mixed Enzyme Ratio of Taste-based Materials Prepared by Enzymatic Hydrolysis of Peanut Meal by Mixture Design[J]. Science and Technology of Food Industry, 2021, 42(23): 184-191. DOI: 10.13386/j.issn1002-0306.2021030209
[3]	WANG Sheng-guang, YU Shuai, MENG Fan-gang, LI Bing-run, SONG Xiao-guang, LIU Guo-fei, WANG Guang-lu, DAI Long, GAO Peng. Study on relative molecular weight distribution and depressor effect of soybean peptide prepared by enzymatic method[J]. Science and Technology of Food Industry, 2018, 39(1): 46-51. DOI: 10.13386/j.issn1002-0306.2018.01.009
[4]	PENG Zhen-fen, WANG Wei, XIE Qian, YE Qing-hua, CHEN Qing-xi, XU Chang-tong. Optimization of testing method for free amino acid of Chinese olive[J]. Science and Technology of Food Industry, 2017, (22): 263-267. DOI: 10.13386/j.issn1002-0306.2017.22.051
[5]	ZHOU Feng-fang, CAI Bin-xin, WU Xin-rui, LUO Fen. Study on hydrolysis condition and molecular weight distribution of ACE inhibitory peptide derived from sea cucumber protein[J]. Science and Technology of Food Industry, 2017, (17): 163-167. DOI: 10.13386/j.issn1002-0306.2017.17.031
[6]	ZHANG Jie, DING Lin, BAI Ge, ZHENG De-juan, CAO Yan-ping. Effect of ultrasonic on molecular weight distribution of papain hydrolyzate[J]. Science and Technology of Food Industry, 2017, (14): 116-120. DOI: 10.13386/j.issn1002-0306.2017.14.023
[7]	YANG Qin, GUO Li-chang, CHEN Hai-qin, ZHANG Hao, CHEN Wei, CHEN Yong- quan. Analysis and characterization of the fatty acids and free amino acids from silkworm and tussah pupa[J]. Science and Technology of Food Industry, 2016, (23): 351-356. DOI: 10.13386/j.issn1002-0306.2016.23.058
[8]	TANG Xiao-yan, ZHENG Hui-na, ZHANG Chao-hua, HAO Ji-ming, ZHANG Jing. Protein composition analysis and molecular weight distribution of Meretrix lusoria[J]. Science and Technology of Food Industry, 2015, (22): 362-366. DOI: 10.13386/j.issn1002-0306.2015.22.066
[9]	LAI Ji- xiang, HE Cong-fen, FANG Yun, ZHAO Ya, WEI Shao-min. Study on molecular weight distribution and antioxidant activity of protein components in germinal black soybean[J]. Science and Technology of Food Industry, 2015, (01): 49-53. DOI: 10.13386/j.issn1002-0306.2015.01.001
[10]	CHANG Ya-nan, ZHAO Gai-ming, LIU Yan-xia, LI Miao-yun, HUANG Xian-qing, SUN Ling-xia. Changes of free amino acids in chicken and its broth during cooking[J]. Science and Technology of Food Industry, 2014, (09): 333-337. DOI: 10.13386/j.issn1002-0306.2014.09.064

Supplements (0)

Cited By

Cited by

Periodical cited type(10)

1.	郁冯艳，付佳伟，从光雷，刘春雪，夏双双，杜莉，李俊波. 发酵饲料在动物生产中应用的研究进展. 饲料研究. 2024(04): 154-157 .
2.	付洋洋，刘禹熙，敖翔，古燕，周建川. 液体发酵饲料的品质管理及其在养猪生产中的应用. 中国畜牧杂志. 2024(08): 55-61 .
3.	蔡英，文洋，张官巨，邵晨阳，桂义国，蓝洪，胡延春. 小分子胶束中药对青峪黑猪肉质的影响研究. 四川畜牧兽医. 2024(10): 28-32 .
4.	申远航，李登云，陈华，姚学丹. 发酵饲料对猪肉品质影响的Meta分析. 动物营养学报. 2023(08): 5374-5383 .
5.	李思懿，粘颖群，谭建庄，卞宝国，杜宏，任向蕾，李春保. 基于电子鼻快速检测生鲜猪肉的异味. 食品工业科技. 2023(20): 338-348 . 本站查看
6.	王娜. 1株益生乳酸菌的分离及其制备的发酵饲料对仔猪生长性能、血清抗氧化与免疫相关指标的影响. 黑龙江畜牧兽医. 2023(19): 93-99 .
7.	牟超. 饲粮中添加白藜芦醇对生长育肥期猪肉品质的影响研究. 畜牧业环境. 2023(06): 5-8 .
8.	李鹏，苏为为，王利平，肖研博，吕珂，王绍怡，岳锋，郭东光，刘兴友. 发酵饲料及其在生猪生产中的应用研究进展. 中国饲料. 2022(16): 119-122 .
9.	宋雪莹. 发酵饲料及其在畜禽生产中应用的研究. 湖南饲料. 2022(06): 47-48 .
10.	王成，靳明亮，单体中，汪以真. 发酵饲料对猪肉品质的影响及机制研究进展. 动物营养学报. 2022(10): 6185-6192 .