Optimization of Extraction Process of Flavonoids from Water Chestnut Shell and Effect on Anti-tumor Cell Proliferation Activity

LEI Qiuqi; YE Shijie; HUANG Yongkang; YANG Guo; ZHOU Min; WANG Hongxun; WANG Limei

doi:10.13386/j.issn1002-0306.2021100178

Volume 43 Issue 14

Jul. 2022

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Science and Technology of Food Industry > 2022 > 43(14): 224-232. > DOI: 10.13386/j.issn1002-0306.2021100178

LEI Qiuqi, YE Shijie, HUANG Yongkang, et al. Optimization of Extraction Process of Flavonoids from Water Chestnut Shell and Effect on Anti-tumor Cell Proliferation Activity[J]. Science and Technology of Food Industry, 2022, 43(14): 224−232. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100178.

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Optimization of Extraction Process of Flavonoids from Water Chestnut Shell and Effect on Anti-tumor Cell Proliferation Activity

LEI Qiuqi¹,
YE Shijie²,
HUANG Yongkang²,
YANG Guo²,
ZHOU Min^{1, 3},
WANG Hongxun^{2, 3},
WANG Limei^{2, 3, ,}

1.
College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
2.
College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China
3.
Hubei Engineering Research Center for Fresh Food, Wuhan 430023, China

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Received Date: October 18, 2021
Available Online: May 08, 2022

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Abstract

Abstract

This experiment was to optimize the extraction process of water chestnut shell flavonoids and investigate its inhibitory effect on the proliferation of human cervical cancer Hela cells. A single factor experiment was used to investigate the effects of extraction time, material-to-liquid ratio, ethanol concentration and extraction temperature on the yield of total flavonoids in water chestnut shells, and response surface analysis was carried out based on the yield of total flavonoids in water chestnut shells to obtain the extraction of water chestnut shell flavonoids. At the same time, the CCK-8 method was used to determine the effect of water chestnut flavonoids on the proliferation of human cervical cancer Hela cells, and to determine its IC₅₀ value to investigate the anti-tumor activity of water chestnut flavonoids. The experimental results showed that the best extraction process of water chestnut shell flavonoids was: extraction time 28 min, material-to-liquid ratio 1:33 g/mL, and ethanol concentration 53%. Under these conditions, the extraction rate of total flavonoids in the crude extract of water chestnut shell could reach 3.455%±0.16%. Incubation with the crude extract or purified solution of water chestnut shell flavonoids at concentrations of 5, 100, 200, 400, 800 µg/mL, all had a proliferation inhibitory effect on Hela cells, and there was a time and concentration dependence. Taking action time 48 h as an example, the IC₅₀ values of the crude extract of water chestnut shell flavonoids, the purified material of water chestnut shell flavonoids, and 5-Fu were 271.46, 268.16, and 152.09 µg/mL, respectively. Therefore, at the same time, the inhibitory effect of water chestnut extract on the proliferation of Hela cells was weaker than that of the positive control 5-Fu.
- water chestnut shell flavonoids,
- process optimization,
- human cervical cancer Hela cells,
- anti-tumor activity

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