Optimization of Sturgeon Cartilage Products and Its Regulation on Human Chondrocyte Inflammation

LIU Hengge; XU Xinxing; BAI Fan; WANG Jinlin; GAO Ruichang; LIU Kang; ZHAO Yuanhui

doi:10.13386/j.issn1002-0306.2021100051

Volume 43 Issue 15

Jul. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(15): 165-174. > DOI: 10.13386/j.issn1002-0306.2021100051

LIU Hengge, XU Xinxing, BAI Fan, et al. Optimization of Sturgeon Cartilage Products and Its Regulation on Human Chondrocyte Inflammation[J]. Science and Technology of Food Industry, 2022, 43(15): 165−174. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100051.

Citation:

PDF (5045 KB)

Optimization of Sturgeon Cartilage Products and Its Regulation on Human Chondrocyte Inflammation

1.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
2.
Quzhou Sturgeon Aquatic Food Science and Technology Development Co., Ltd., Quzhou 324002, China
3.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China

More Information

Received Date: October 11, 2021
Available Online: June 18, 2022

Graphical Abstract

Abstract

Abstract

The purpose of this paper was to prepare a sturgeon cartilage product from sturgeon cartilage and explore its effect on inflammation of human chondrocytes. A kind of sturgeon cartilage product was prepared by thermal liquefaction of sturgeon cartilage and dragon tendon at high temperature and high pressure. The optimum preparation conditions were determined by single factor and orthogonal test. Human chondrocytes were cultured by preparing drug-containing serum and the effects of sturgeon cartilage products on Interleukin 1β (IL-1β)-induced cell inflammatory model were investigated by MTT, Alsinland staining and ELISA. The results showed that the best preparation conditions of sturgeon cartilage products were as follows: Hot liquefaction temperature was 120 ℃, hot liquefaction time was 20 min, boiling temperature was 80 ℃, boiling time was 30 min. Under this condition, the sensory score of the product was 59.69. The drug-containing serum of sturgeon cartilage products group had no cytotoxicity and could stimulate chondrocytes to secrete collagen and proteoglycan and other extracellular matrix. Meanwhile, the product could significantly increase the proliferation activity of inflammatory cells by 156% (P<0.05), and significantly inhibited the expression levels of NO, INOS, IL-6, COX-2 and PGE2 in the inflammatory chondrocytes (P<0.05).
- sturgeon,
- cartilage,
- osteoarthritis,
- chondrocyte,
- inflammatory factor,
- process optimization

FullText(HTML)

References (38)

References

[1]	唐淑玮, 高瑞昌, 曾名湧, 等. 鲟鱼鱼糜凝胶形成过程中的物理化学变化[J]. 食品科学,2019,40(7):82−87. [TANG S W, GAO R C, ZENG M Y, et al. Physicochemical changes during gel formation of sturgeon surimi[J]. Food Science,2019,40(7):82−87. doi: 10.7506/spkx1002-6630-20180320-253 TANG S W, GAO R C, ZENG M Y, et al. Physicochemical changes during gel formation of sturgeon surimi[J]. Food Science, 2019, 40(7): 82-87. doi: 10.7506/spkx1002-6630-20180320-253
[2]	李军, 李平兰, 高亮, 等. 萝卜籽蛋白提取物对托盘包装鲟鱼片冷藏品质的影响[J]. 食品科学,2018,39(9):224−231. [LI J, LI P L, GAO L, et al. Effect of radish seed protein extract on refrigerated quality of tray packed sturgeon fillet[J]. Food Science,2018,39(9):224−231. doi: 10.7506/spkx1002-6630-201809034 LI J, LI P L, GAO L, et al. Effect of radish seed protein extract on refrigerated quality of tray packed sturgeon fillet[J]. Food Science, 2018, 39(9): 224-231. doi: 10.7506/spkx1002-6630-201809034
[3]	周艳丽, 陈世杰, 劳文艳, 等. 氨基葡萄糖和骨碎补联用对大鼠慢性骨关节炎的干预作用及机制[J]. 食品科学,2018,39(3):213−221. [ZHOU Y L, CHEN S J, LAO W Y, et al. Intervention effect and mechanism of glucosamine combined with bone crusher on chronic osteoarthritis in rats[J]. Food Science,2018,39(3):213−221. doi: 10.7506/spkx1002-6630-201803033 ZHOU Y L, CHEN S J, LAO W Y, et al. Intervention effect and mechanism of glucosamine combined with bone crusher on chronic osteoarthritis in rats[J]. Food Science, 2018, 39(3): 213-221. doi: 10.7506/spkx1002-6630-201803033
[4]	TANG Y H, LI Y F, XIN D W, et al. Icariin alleviates osteoarthritis by regulating autophagy of chondrocytes by mediating PI3K/AKT/mTOR signaling[J]. Bioengineered,2021,12(1):2984−2999. doi: 10.1080/21655979.2021.1943602
[5]	EBATA T, TERKAWI M A, HAMASAKI M, et al. Flightless I is a catabolic factor of chondrocytes that promotes hypertrophy and cartilage degeneration in osteoarthritis[J]. Science,2021,24(6):102643.
[6]	杭兴伟, 增田泰伸, 木村守, 等. 硫酸氨基葡萄糖对口服透明质酸缓解小鼠佐剂性关节炎的增效作用[J]. 食品科学,2015,36(5):189−194. [HANG X W, ZENGTIANTAI S, MUCUN S, et al. Synergism of glucosamine sulfate in relieving effect of hyaluronic acid by oral administration on mice with adjuvant arthritis[J]. Food Science,2015,36(5):189−194. doi: 10.7506/spkx1002-6630-201505036 HANG X W, ZENGTIANTAI S, MUCUN S, et al. Synergism of glucosamine sulfate in relieving effect of hyaluronic acid by oral administration on mice with adjuvant arthritis[J]. Food Science, 2015, 36(5): 189-194 doi: 10.7506/spkx1002-6630-201505036
[7]	RAFAEL D R M, LUCAS F D F, JOSE F S D L, et al. Biofat grafts as an orthobiologic tool in osteoarthritis: An update and classification proposal[J]. World Journal of Meta Analysis,2021,9(1):29−39. doi: 10.13105/wjma.v9.i1.29
[8]	WANG K Y, BAI F, ZHOU X D, et al. Characterization of chondroitin sulfates isolated from large hybrid sturgeon cartilage and their gastroprotective activity against ethanol-induced gastric ulcers[J]. Food Chemistry,2021,363:130436. doi: 10.1016/j.foodchem.2021.130436
[9]	武瑞赟, 杜怡丽, 张金兰, 等. 乳杆菌发酵鲟鱼骨酶解液条件优化及营养成分分析[J]. 食品科学,2019,40(10):57−63. [WU R Y, DU Y L, ZHANG J L, et al. Optimization of fermentation conditions for higher viable count of lactobacillus paraplantarum in enzymatic hydrolysate of sturgeon cartilage and changes in nutrient components before and after fermentation[J]. Food Science,2019,40(10):57−63. doi: 10.7506/spkx1002-6630-20181210-123 WU R Y, DU Y L, ZHANG J L, et al. Optimization of fermentation conditions for higher viable count of lactobacillus paraplantarum in enzymatic hydrolysate of sturgeon cartilage and changes in nutrient components before and after fermentation[J]. Food Science, 2019, 40(10): 57-63 doi: 10.7506/spkx1002-6630-20181210-123
[10]	李崇阳, 李艳, 牟德华. 氨基葡萄糖硫酸软骨素促进骨骼健康作用研究进展[J]. 食品科学,2015,36(23):382−385. [LI C Y, LI Y, MOU D H. Advances in research on the promotion effect of glucosamine and chondroitin sulfate on bone health[J]. Food Science,2015,36(23):382−385. doi: 10.7506/spkx1002-6630-201523068 LI C Y, LI Y, MOU D H. Advances in research on the promotion effect of glucosamine and chondroitin sulfate on bone health[J]. Food Science, 2015, 36(23): 382-385 doi: 10.7506/spkx1002-6630-201523068
[11]	许蓉, 郑淋, 赵谋明. 温度和pH值对鸡胸软骨Ⅱ型胶原蛋白的结构、黏度和热稳定性的影响[J]. 食品科学,2020,41(14):66−71. [XU R, ZHENG L, ZHAO M M. Effect of temperature and pH on the structure, viscosity and thermal stability of type II collagen from chicken cartilage[J]. Food Science,2020,41(14):66−71. doi: 10.7506/spkx1002-6630-20190624-294 XU R, ZHENG L, ZHAO M M. Effect of temperature and pH on the structure, viscosity and thermal stability of type II collagen from chicken cartilage[J]. Food Science, 2020, 41(14): 66-71. doi: 10.7506/spkx1002-6630-20190624-294
[12]	RUI F, KANG J W, HAO Y T, et al. Undenatured type II collagen prevents and treats osteoarthritis and motor function degradation in T2DM patients and db/db mice[J]. Food & Function,2021,12(3):4373−4391.
[13]	CAO F, CHEN Y, WANG X, et al. Therapeutic effect and potential mechanisms of intra-articular injections of miR-140-5p on early-stage osteoarthritis in rats[J]. International Immunopharmacology,2021,96(7):107786.
[14]	彭海川, 钱琴, 母运龙, 等. 不同烹饪方式处理的鲟鱼肉营养成分和风味比较[J/OL]. 现代食品科技: 1−9 [2021-11-15]. https://doi.org/10.13982/j.mfst.1673-9078.2022. 2.0585 PENG H C, QIAN Q, MU Y L, et al. Comparison of nutrient composition and flavor of sturgeon meat processed by different cooking methods [J/OL]. Modern Food Science and Technology: 1−9 [2021-11-15]. https://doi.org/10.13982/j.mfst.1673-9078.2022.2.0585.
[15]	向晨曦, 李钰金, 高瑞昌, 等. 打印参数对未漂洗鲟鱼糜凝胶3D打印成型效果的影响[J/OL]. 食品工业科技: 1−15 [2021-11-15]. https://doi.org/10.13386/j.issn1002-0306.2021040100. XIANG C X, LI Y J, GAO R C, et al. Effect of printing parameters on 3D printing effect of unrinsed sturgeon surimi gel[J/OL]. Science and Technology of Food Industry: 1−15 [2021-11-15]. https://doi.org/10.13386/j.issn1002-0306.2021040100.
[16]	黄卉, 魏涯, 李来好, 等. 季节变化对杂交鲟鱼肉营养成分的影响[J]. 食品工业科技,2021,42(7):360−365. [HUANG H, WEI Y, LI L H, et al. Effects of seasonal variation on nutrient composition of meat of hybrid sturgeon[J]. Science and Technology of Food Industry,2021,42(7):360−365. HUANG H, WEI Y, LI L H, et al. Effects of seasonal variation on nutrient composition of meat of hybrid sturgeon[J]. Science and Technology of Food Industry, 2021, 42(7): 360-365.
[17]	武瑞赟, 杜怡丽, 黄雨霞, 等. 鲟鱼软骨酶解条件优化及酶解物的体外抗肿瘤活性[J]. 中国农业大学学报,2020,25(1):131−141. [WU R Y, DU Y L, HUANG Y X, et al. Optimization of enzymolysis conditions of Sturgeon cartilage and in vitro antitumor activity of enzymolysis products[J]. Journal of China Agricultural University,2020,25(1):131−141. doi: 10.11841/j.issn.1007-4333.2020.01.15 WU R Y, DU Y L, HUANG Y X, et al. Optimization of enzymolysis conditions of Sturgeon cartilage and in vitro antitumor activity of enzymolysis products[J]. Journal of China Agricultural University, 2020, 25(1): 131-141. doi: 10.11841/j.issn.1007-4333.2020.01.15
[18]	徐阳, 孙强, 李静, 等. 大鲵胶原蛋白多肽口服液的开发研究[J]. 食品工业,2016,37(3):1−5. [XU Y, SUN Q, LI J, et al. Development and research for andrias davidianus collagen peptide oral liquid[J]. The Food Industry,2016,37(3):1−5. XU Y, SUN Q, LI J, et al. Development and research for andrias davidianus collagen peptide oral liquid[J]. The Food Industry, 2016, 37(3): 1-5
[19]	程雨晴, 解万翠, 杨锡洪, 等. 鱼鳞胶原蛋白凝冻休闲食品的制备及其优化[J]. 农产品加工(学刊),2014(5):12−14. [CHENG Y Q, XIE W C, YANG X H, et al. Preparation and optimization of fish scale collagen frozen snack food[J]. Agricultural Processing (Journal),2014(5):12−14. CHENG Y Q, XIE W C, YANG X H, et al. Preparation and optimization of fish scale collagen frozen snack food[J]. Agricultural Processing (Journal), 2014(5): 12-14.
[20]	陆剑锋, 万全, 殷章敏, 等. 中华鳖裙边胶原蛋白的提取及其特征[J]. 水产学报,2010,34(6):981−988. [LU J F, WAN Q, YIN Z M, et al. Extraction and characteristics of collagen from skirt edge of China turtles[J]. Journal of Fisheries of China,2010,34(6):981−988. LU J F, WAN Q, YIN Z M, et al. Extraction and characteristics of collagen from skirt edge of China turtles[J]. Journal of Fisheries of China, 2010, 34(6): 981-988.
[21]	LI J W, WANG R L, XU J, et al. Methylene blue prevents osteoarthritis progression and relieves pain in rats via upregulation of Nrf2/PRDX1[J]. Acta Pharmacologica Sinica,2021:1−12.
[22]	李梦秋,黄娟,邹国发,等. 高良姜含药血清及主要成分对TRPA1信号通路的影响[J]. 中国药理学通报,2021,37(10):1443−1449. [LI M Q, HUANG J, ZOU G F, et al. Effects of galangal serum and its main components on TRPA1 signaling pathway[J]. Chinese Pharmacological Bulletin,2021,37(10):1443−1449. LI M Q, HUANG J, ZOU G F, et al. Effects of galangal serum and its main components on TRPA1 sig- naling pathway[J]. Chinese Pharmacological Bulletin, 2021,37(10):1443-1449.
[23]	杨大俏, 王锦旭, 李来好, 等. 近江牡蛎多糖的结构鉴定及免疫调节能力分析[J]. 食品科学,2020,41(10):38−46. [YANG D Q, WANG J X, LI L H, et al. Structural analysis and immunoregulatory activity of polysaccharides from Crassostrea rivularis[J]. Food Science,2020,41(10):38−46. doi: 10.7506/spkx1002-6630-20190524-295 YANG D Q, WANG J X, LI L H, et al. Structural analysis and immunoregulatory activity of polysaccharides from Crassostrea rivularis[J]. Food Science, 2020, 41(10): 38-46 doi: 10.7506/spkx1002-6630-20190524-295
[24]	YANG J, YAO L T, LI Y X, et al. Interleukin-35 regulates angiogenesis through P38 mitogen-activated protein kinase signaling pathway in Interleukin-1β-Stimulated SW1353 cells and cartilage bioinformatics analysis[J]. Journal of Interferon & Cytokine Research: The Official Journal of the International Society for Interferon and Cytokine Research,2021,41(5):164−171.
[25]	孙群周. 狗脊多糖通过miR-181c调控IL-1β介导的骨关节炎软骨细胞增殖和凋亡[J]. 中国老年学杂志,2021,41(11):2398−2402. [SUN Q Z. MiR-181c regulates the proliferation and apoptosis of osteoarthritis chondrocytes mediated by IL-1β[J]. Chinese Journal of Gerontology,2021,41(11):2398−2402. doi: 10.3969/j.issn.1005-9202.2021.11.046 SUN Q Z. MiR-181c regulates the proliferation and apoptosis of osteoarthritis chondrocytes mediated by IL-1β[J]. Chinese Journal of Gerontology, 2021, 41(11): 2398-2402 doi: 10.3969/j.issn.1005-9202.2021.11.046
[26]	虞舒蓓, 林淑. 依达拉奉对脂多糖激活的BV-2小胶质细胞中促炎细胞因子的影响[J]. 中国临床药理学杂志,2019,35(12):1244−1246. [YU S P, LIN S. Effects of edaravone on pro-inflammatory cytokines in lipopolysaccharide-activated BV-2 microglia[J]. The Chinese Journal of Clinical Pharmacology,2019,35(12):1244−1246. YU S P, LIN S. Effects of edaravone on pro-inflammatory cytokines in lipopolysaccharide-activated BV-2 microglia[J]. The Chinese Journal of Clinical Pharmacology, 2019, 35(12): 1244-1246.
[27]	中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. GB 10136-2015动物性水产制品[S]. 北京: 中国标准出版社, 2007. General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of China, GB 10136-2015 Aquatic products of animal origin[S]. Beijing: China Standard Press, 2007
[28]	陈俊, 张娜玲, 邹冰, 等. 糖尿病和高糖血症在骨性关节炎中的作用[J]. 局解手术学杂志,2020,29(7):590−593. [CHEN J, ZHANG N L, ZOU B, et al. Role of diabetes or hyperglycemia in osteoarthritis[J]. Journal of Regional Anatomy and Operative Surgery,2020,29(7):590−593. CHEN J, ZHANG N L, ZOU B, et al. Role of diabetes or hyperglycemia in osteoarthritis[J]. Journal of Regional Anatomy and Operative Surgery, 2020, 29(7): 590-593
[29]	杨舒乔,王迪,高彦祥. 非变性Ⅱ型胶原蛋白研究进展[J]. 食品科学,2021,42(17):343−349. [YANG S Q, WANG D, GAO Y X. The research of undenatured type II collagen[J]. Food Science,2021,42(17):343−349. YANG S Q, WANG D, GAO Y X. The research of undenatured type II collagen[J]. Food Science,2021,42(17):343-349.
[30]	FERNANDEZ M S, GONZALEZ C A, MUNOZ F, et al. Glucosamine and chondroitin sulfate: Is there any scientific evidence for their effectiveness as disease-modifying drugs in knee osteoarthritis preclinical studies?—A systematic review from 2000 to 2021[J]. Animals,2021,11(6):1608−1608. doi: 10.3390/ani11061608
[31]	于庆贺, 曲戎梅, 张国炜, 等. 3种成软骨诱导培养基对单层培养和微球培养的人脂肪干细胞成软骨效果比较[J]. 中国临床解剖学杂志,2021,39(1):71−75. [YU Q H, QU R M, ZHANG G W, et al. Comparison of chondrogenic effects of three kinds of chondrogenic induction media on human adipose-derived stem cells in monolayer and microsphere cultured[J]. Chinese Journal of Clinical Anatomy,2021,39(1):71−75. YU Q H, QU R M, ZHANG G W, et al. Comparison of chondrogenic effects of three kinds of chondrogenic induction media on human adipose-derived stem cells in monolayer and microsphere cultured[J]. Chinese Journal of Clinical Anatomy, 2021, 39(1): 71-75
[32]	吴松, 王鑫, 张彦, 等. 高糖高脂饮食对兔膝关节软骨形态的影响[J]. 中国组织工程研究与临床康复,2009,13(33):6433−6438. [WU S, WANG X, ZHANG Y, et al. Effect of high-sugar and high-fat diet on the morphology of rabbit knee articular cartilage[J]. Chinese Journal of Tissue Engineering Research,2009,13(33):6433−6438. WU S, WANG X, ZHANG Y, et al. Effect of high-sugar and high-fat diet on the morphology of rabbit knee articular cartilage[J]. Chinese Journal of Tissue Engineering Research, 2009, 13(33): 6433-6438.
[33]	OHTSUKI T, HATIPOGLU O F, ASANO K, et al. Induction of CEMIP in chondrocytes by inflammatory cytokines: Underlying mechanisms and potential involvement in osteoarthritis[J]. International Journal of Molecular Sciences,2020,21(9):3140. doi: 10.3390/ijms21093140
[34]	PAN T T, PAN F, GAO W, et al. Involvement of macrophages and spinal microglia in osteoarthritis pain[J]. Current Rheumatology Reports,2021,23(5):1−7.
[35]	HONG L, SHARP T, KHORSAND B, et al. Micro RNA-200c represses IL-6, IL-8, and CCL-5 expression and enhances osteogenic differentiation[J]. PloS One,2016,11(8):1−16.
[36]	PHAM T M, ERICHSEN J L, KOWAL J M, et al. Elevation of pro-inflammatory cytokine levels following intra-articular fractures-a systematic review[J]. Cells,2021,10(4):1−9.
[37]	FORTUNA R, HART D A, SHARKEY K A, et al. Effect of a prebiotic supplement on knee joint function, gut microbiota, and inflammation in adults with co-morbid obesity and knee osteoarthritis: study protocol for a randomized controlled trial[J]. Trials,2021,22(1):255. doi: 10.1186/s13063-021-05212-w
[38]	JIN F, LIAO L, ZHU Y. MiR-467b alleviates lipopolysaccharide-induced inflammation through targeting STAT1 in chondrogenic ATDC5 cells[J]. International Journal of Immunogenetics,2021,48(5):435−442. doi: 10.1111/iji.12534

[1]	JIN Zishuang, CHEN Jiao, MA Zhiqian, ZUO Jia, WU Yiming, MEI Lin. Effect of Ultra High Pressure Combined with Partial Freezing on the Storage Quality of Chicken Soup[J]. Science and Technology of Food Industry, 2024, 45(15): 332-341. DOI: 10.13386/j.issn1002-0306.2023090187
[2]	MENG Di, JIAO He, ZHAO Anqi, HAN Ying, HE Xue, LI Pengxia, HU Huali. Effect of Vacuum Pre-cooling on the Circulation and Shelf Quality of Postharvest Baby Cabbage[J]. Science and Technology of Food Industry, 2024, 45(9): 296-308. DOI: 10.13386/j.issn1002-0306.2023060008
[3]	ZHAO Zhibing, HUANG Tingting, LÜ Jiahan, DU Xiaoji, ZHANG Changfeng, ZHANG Yu, CAO Sen. Effect of Melatonin Coupling with Eugenol Treatment on Storage Quality of Red Amygdalus persica[J]. Science and Technology of Food Industry, 2022, 43(23): 341-346. DOI: 10.13386/j.issn1002-0306.2022070080
[4]	FU Baoshang, DING Ruosong, SHANG Shan, DONG Xiuping, QI Libo, YU Bo. Effects of Different Preservatives on the Storage Quality of Roasted Flake[J]. Science and Technology of Food Industry, 2021, 42(20): 301-308. DOI: 10.13386/j.issn1002-0306.2021010090
[5]	GUO Shuting, XUE Jianyi, LI Feng, ZHAO Botao, WANG Yongli. Effects of Vacuum Precooling Pressure on Postharvest Preservative Quality of Spinach[J]. Science and Technology of Food Industry, 2021, 42(5): 265-269. DOI: 10.13386/j.issn1002-0306.2020050230
[6]	LI Wenjun, LIU Guangqin, WANG Chengzhang, YE Jianzhong, WANG Zijin. Effects of Compound Preservative on the Postharvest Quality of Carya illinoensis during Storage[J]. Science and Technology of Food Industry, 2021, 42(3): 258-264,271. DOI: 10.13386/j.issn1002-0306.2020040215
[7]	DUAN Zhou-wei, XIE Hui, WANG Shi-ping, HE Ai, DOU Zhi-hao. Effect of vacuum pre-cooling treatment on storage quality of Australia Mango[J]. Science and Technology of Food Industry, 2017, (03): 326-330. DOI: 10.13386/j.issn1002-0306.2017.03.055
[8]	LIAO Cai-hu, SHAN Bin, ZHONG Rui-min, HUANG Guo-qing, LAI Wang-hang. Effect of different final temperature on vacuum cooled fresh beef's quality[J]. Science and Technology of Food Industry, 2013, (24): 334-338. DOI: 10.13386/j.issn1002-0306.2013.24.084
[9]	Effect of oligochitosan on storage qualities of apricot fruit[J]. Science and Technology of Food Industry, 2012, (17): 353-356. DOI: 10.13386/j.issn1002-0306.2012.17.006
[10]	Effect of salicylic acid treatment on storage quality of apricot fruits[J]. Science and Technology of Food Industry, 2012, (15): 335-337. DOI: 10.13386/j.issn1002-0306.2012.15.094

Supplements (0)

Cited By

Cited by

Periodical cited type(4)

1.	刘子夜，武琳霞，刘美玉，王蒙. 山东地区不同品种樱桃营养品质评价. 食品安全质量检测学报. 2025(02): 35-45 .
2.	宋莎，刘方利，解璞，王恩权，张绿萍，金吉林. 玛瑙红樱桃胚败育果实和正常发育果实外观形态比较. 中国果树. 2025(03): 42-46 .
3.	陈丽娟，王东，李洪雯，刘佳，张国薇. 中国樱桃不同品种（优系）果实品质分析. 热带作物学报. 2024(03): 514-523 .
4.	赵新玉，杜文瑜，谭梦男，郑晓冬，刘雪梅，闫新焕. 基于气相离子迁移谱技术分析延安市不同县区的富士苹果挥发性成分的差异. 食品安全质量检测学报. 2024(12): 240-250 .