XIA Shiqi, WANG Peiling, CHEN Shangxing, et al. Stability of Citral Sustained-release Preparation and Its Inhibitory Effect on Aspergillus flavus[J]. Science and Technology of Food Industry, 2022, 43(8): 85−92. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070371.
Citation: XIA Shiqi, WANG Peiling, CHEN Shangxing, et al. Stability of Citral Sustained-release Preparation and Its Inhibitory Effect on Aspergillus flavus[J]. Science and Technology of Food Industry, 2022, 43(8): 85−92. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070371.

Stability of Citral Sustained-release Preparation and Its Inhibitory Effect on Aspergillus flavus

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  • Received Date: August 02, 2021
  • Available Online: February 13, 2022
  • To provide data support for the development of green, efficient and safe sustained-release bacteriostatic agent of citral, the stability of the two kinds of sustained-release preparation of citral liposome (CL) and citral liposome-chitosan (CL-CS) and the bacteriostatic effect and mechanism of Aspergillus flavus were investigated. Under the condition of different temperature and pH, the appearance, particle size, Zeta potential and retention rate were studied to explore the storage stability. The instantaneous bacteriostasis rate and long-term bacteriostasis rate of citral sustained-release were determined by spore counting method. The bacteriostatic mechanism of citral sustained-release preparations was revealed according to the changes of cell permeability. The results showed that the two citral sustained-release preparations had uniform nano-sized particle sizes. After chitosan modification, the Zeta potential of the system changed from −16.63±1.67 to 35.72±3.29. After 28 days of storage at 4 ℃, the change of particle size and Zeta potential was small, and the retention rate was higher, CL-CS was more stable than CL. At pH4~6, the two sustained-release preparations were more stable, and the particle sizes of CL and CL-CS were about 150 and 200 nm. The instantaneous inhibitory EC50 of CL and CL-CS against Aspergillus flavus within 48 h were 77.88 and 68.20 mg·L−1, respectively. After 48 h of culture, the inhibitory rates of CL and CL-CS were 67.69% and 82.89%, respectively, while citral was only 30.26%. The results showed that CL-CS had good bacteriostatic effect and long-term bacteriostatic effect. The extracellular conductivity and nucleic acid content of Aspergillus flavus both increased after treatment with the two kinds of sustained release preparations, indicating that the permeability of cell membrane increased and the action time was shorter than that of free citral. In conclusion, the two kinds of sustained-release preparations have good stability. They inhibited the growth of Aspergillus flavus by the change of cell permeability and the leak of insoluble substance. The properties of CL-CS was better than CL, and it would have the potential to be developed as plant source mildew inhibitor.
  • [1]
    杨博磊, 耿海荣, 王刚, 等. 我国花生土壤黄曲霉菌分布与产后花生黄曲霉毒素污染相关性研究[J]. 核农学报,2021,35(4):863−869. [YANG B L, GENG H R, WANG G, et al. The relationship between Asperillus flavus in peanut soil and aflatoxin contamination of peanut in China[J]. Journal of Nuclear Agricultural Sciences,2021,35(4):863−869. doi: 10.11869/j.issn.100-8551.2021.04.0863
    [2]
    胡佳哲, 赖宇红, 陈浩桉. 中药材中常见真菌毒素污染状况及分析方法研究进展[J]. 海峡药学,2019,31(1):1−5. [HU J Z, LAI Y H, CHEN H A. Research progress of the pollution status and analysis methods of common fungal toxins in chinese medicinal herbs[J]. Strait Pharmaceutical Journal,2019,31(1):1−5. doi: 10.3969/j.issn.1006-3765.2019.01.001
    [3]
    贾睿, 蔡丹, 刘景圣, 等. 天然植物源抑菌活性成分研究进展[J]. 食品工业,2020,41(10):283−287. [JIA R, CAI D, lIU J S, et al. Research progress of antibacterial active components from natural plants[J]. The Food Industry,2020,41(10):283−287.
    [4]
    王龑, 林威, 俞根荣, 等. 植物精油在食品防霉保质中应用的研究进展[J]. 核农学报,2021,35(5):1170−1177. [WANG Y, LIN W, YU G R, et al. Advance in anti-fungus application of plant essential oil in food preservation[J]. Journal of Nuclear Agricultural Sciences,2021,35(5):1170−1177. doi: 10.11869/j.issn.100-8551.2021.05.1170
    [5]
    XIA S Q, LIN H, ZHU P L, et al. Inhibitory effects of Litsea cubeba oil and its active components on Aspergillus flavus[J]. Journal of Food Quality,2020:1−9.
    [6]
    向俊, 樊蕊, 王磊, 等. 柠檬醛微胶囊化研究进展[J]. 中国食品添加剂,2014(9):176−183. [XIANG J, FAN R, WANG L, et al. Research progress of citral microencapsulation[J]. China Food Additives,2014(9):176−183. doi: 10.3969/j.issn.1006-2513.2014.09.020
    [7]
    MIRANDA-CADEN K, DIAS M, COSTA-BARBOSA A, et al. Development and characterization of monoolein-based liposomes of carvacrol, cinnamaldehyde, citral or thymol with anti-candida activities[J]. Antimicrobial Agents and Chemotherapy,2021,65(6):1−21.
    [8]
    徐伟丽, 朱元昊, 张玉琪, 等. α-生育酚脂质体的配方优化及其稳定性分析[J]. 哈尔滨工业大学学报,2020,52(12):116−125. [XU W L, ZHU Y H, ZHANG Y Q, et al. Formulation optimization and stability analysis of α-tocopherol liposomes[J]. Journal of Harbin Institute of Technology,2020,52(12):116−125. doi: 10.11918/201907024
    [9]
    郝静梅, 孙志高, 盛冉, 等. 柠檬烯纳米脂质体的制备及其性质测定[J]. 食品与发酵工业,2018,44(4):173−179. [HAO J M, SUN Z G, PENG R, et al. Preparation and characterization of limonene nanoliposomes[J]. Food and Fermentation Industries,2018,44(4):173−179.
    [10]
    程铭, 焦文佳, 陶冶, 等. 壳聚糖修饰植物甾醇脂质体的制备及稳定性研究[J]. 中国粮油学报,2018,33(8):75−80,86. [CHENG M, JIAO W J, TAO Y, et al. Effects of CL and CL-CS on extracellular nucleic acid of Aspergillus flavus[J]. Journal of the Chinese Cereals and Oils Association,2018,33(8):75−80,86. doi: 10.3969/j.issn.1003-0174.2018.08.013
    [11]
    PEREIRA O, MENDES J M, LIMA I O, et al. Antifungal activity of geraniol and citronellol, two monoterpenes alcohols, against Trichophyton rubrum involves inhibition of ergosterol biosynthesis[J]. Pharmaceutical Biology,2015,53(2):228−234. doi: 10.3109/13880209.2014.913299
    [12]
    张雪婧. 刺激释放型豆蔻精油纳米脂质体的制备及在肉类食品中的应用[D]. 苏州: 江苏大学, 2016.

    ZHANG X J. Preparation of Stimulus-releasing nano-liposomes of cardamom essential oil and its application in meat products[D]. Suzhou: Jiangsu University, 2016.
    [13]
    MA Y F, WANG Z, ZHAO W, et al. Enhanced bactericidal potency of nanoliposomes by modification of the fusion activity between liposomes and bacterium[J]. Int J Nanomed,2013,8:2351−2360.
    [14]
    JOSHI O, CHU L, MC G J, et al. Adsorption and function of recombinant Factor VIII at the air-water interface in the presence of Tween 80[J]. J Pharm Sci,2009,98(9):3099−3107. doi: 10.1002/jps.21569
    [15]
    TASI L, LIU D, CHEN W. Microcalorimetric Investigation of the interaction of polysorbate surfactants with unilamellar phosphatidylcholines liposomes[J]. Colloids and Surfaces A:Physicochemical and Engineering Aspects,2003,213(1):7.
    [16]
    PUSK I, CSEMPESZ F. Influence of cyclodextrins on the physical stability of DPPc-liposomes[J]. Colloids and Surfaces B:Biointerfaces,2007,58(2):218−224. doi: 10.1016/j.colsurfb.2007.03.011
    [17]
    刘丽. 肉桂醛脂质体的制备与稳定性研究[D]. 无锡: 江南大学, 2017.

    LIU L. Preparation and stability of cinnamaldehyde liposomes[D]. Wuxi: Jiangnan University, 2017.
    [18]
    CHEN E, WU S, MCCLEMENTS C D, et al. Influence of pH and cinnamaldehyde on the physical stability and lipolysis of whey protein isolate-stabilized emulsions[J]. Food Hydrocolloids,2017(69):103−110.
    [19]
    ZIDOVSKA A, EWERT K, QUISPE J, et al. The effect of salt and ph on block liposomes studied by cryogenic transmission electron microscopy[J]. Biochimica et Biophysica Acta: Biomembranes,2009,1788(9):1869−1876. doi: 10.1016/j.bbamem.2009.06.013
    [20]
    成方圆. 肉桂醛脂质体及其壳聚糖修饰物的制备与抑菌性研究[D]. 无锡: 江南大学, 2018.

    CHEN F Y. Preparation and antibacterial activity of cinnamaldehyde liposome and its chitosan modifier[D]. Wuxi: Jiangnan University, 2018.
    [21]
    蓝蔚青, 杨歆, 王蒙, 等. 壳聚糖对腐生葡萄球菌的作用机制[J]. 广东海洋大学学报,2021,41(1):63−71. [LAN W Q, YANG X, WANG M, et al. Mechanism of chitosan against Staphylococcus saprophytic[J]. Journal of Guangdong Ocean University,2021,41(1):63−71. doi: 10.3969/j.issn.1673-9159.2021.01.001
    [22]
    郭丹, 雷潇, 王鹏, 等. 山苍子油主成分及其衍生物对植物病原菌的抑制作用[J]. 江西农业大学学报,2016,38(2):283−289. [GUO D, LEI X, WANG P, et al. Inhibition of main components and derivatives of Litsea cubeba oil on plant pathogens[J]. Journal of Jiangxi Agricultural University,2016,38(2):283−289.
    [23]
    魏娟. 柠檬醛和肉桂醛抑制接骨木镰刀菌生长和产毒的机理[D]. 兰州: 甘肃农业大学, 2020.

    WEI J. Mechanism of citral and cinnamaldehyde inhibiting the growth and toxicity of Fusarium elderium[D]. Lanzhou: Gansu Agricultural University, 2020.
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