Dynamic Changes in Volatile Flavor Components during Enzymatic Hydrolysis of Walnut (<i>Juglans regia</i> L.) Protein

LI Kai; FAN Yuchuan; DING Lan; ZENG Ming; SONG Hao

doi:10.13386/j.issn1002-0306.2021070087

Volume 43 Issue 6

Mar. 2022

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Science and Technology of Food Industry > 2022 > 43(6): 41-49. > DOI: 10.13386/j.issn1002-0306.2021070087

LI Kai, FAN Yuchuan, DING Lan, et al. Dynamic Changes in Volatile Flavor Components during Enzymatic Hydrolysis of Walnut (Juglans regia L.) Protein[J]. Science and Technology of Food Industry, 2022, 43(6): 41−49. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070087.

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Dynamic Changes in Volatile Flavor Components during Enzymatic Hydrolysis of Walnut (Juglans regia L.) Protein

LI Kai^{1, 2,},
FAN Yuchuan^{1, 2},
DING Lan^{1, 2},
ZENG Ming^{1, 2},
SONG Hao^{1, 2, ,}

1.
Beijing Industrial Technology Research Institute, Beijing 101111, China
2.
Beijing Food Industry Research Institute, Beijing 100075, China

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Received Date: July 06, 2021
Available Online: January 14, 2022

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Abstract

Abstract

In order to study the dynamic changes in volatile flavor components of walnut (Juglans regia L.) protein during enzymatic hydrolysis, single-enzyme hydrolysis was carried out using Protamex^® protease. The headspace solid-phase microextraction and gas chromatography-mass spectrometry were used to analyze the volatile chemical components of the enzymatic hydrolysis solution at different time points, and the results were used to carried out relative odor activity analysis and principal component analysis (PCA). The results showed that fifty-five and sixty-eight aroma substances were detected before and after enzymolysis, including aldehydes, alcohols, ketones, esters and furans. With the progress of enzymatic hydrolysis, the key aroma components were gradually transformed from twenty-one compounds such as n-valeraldehyde, 1-octene-3-ol and isovaleraldehyde before enzymolysis into thirteen compounds such as isovaleraldehyde, dimethyl disulfide and (2E,4E)-decadienal. The PCA results showed that the aroma characteristics tended to be stable after enzymatic hydrolysis for 3 h, in addition, the aroma structure change of the enzymatic hydrolysis solution was mainly affected by the concentration change of 2-ethyl-1-hexanol, 2-ethyl furan, (2E,4E)-decadienal, 2-pentyl furan, (Z)-4-heptenal and dimethyl disulfide. These research results would provide a theoretical basis for the control of flavor quality during the preparation of walnut peptides by enzymolysis.
- walnut protein,
- enzymolysis,
- headspace solid-phase microextraction,
- gas chromatography-mass spectrometry,
- volatile flavor components

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References

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