Fermentation Optimization of Kelp-Apple Compound Juice Beverage and the Antioxidant Activity of Its Fermentation System

LIU Mao; FU Xiaoting; WANG Lei; XU Jiachao; GAO Xin

doi:10.13386/j.issn1002-0306.2021060184

Volume 43 Issue 4

Feb. 2022

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Science and Technology of Food Industry > 2022 > 43(4): 214-220. > DOI: 10.13386/j.issn1002-0306.2021060184

LIU Mao, FU Xiaoting, WANG Lei, et al. Fermentation Optimization of Kelp-Apple Compound Juice Beverage and the Antioxidant Activity of Its Fermentation System[J]. Science and Technology of Food Industry, 2022, 43(4): 214−220. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060184.

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Fermentation Optimization of Kelp-Apple Compound Juice Beverage and the Antioxidant Activity of Its Fermentation System

College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China

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Received Date: June 22, 2021
Available Online: December 17, 2021

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Abstract

Abstract

In this study, kelp juice, apple juice and sugar were used as the main raw materials, and Lactobacillus plantarum was applied as the fermentation strain, to develop a fermented kelp-apple compound juice beverage with excellent flavor and natural nutrition. Based on sensory scores, the process optimization of the compound fruit juice beverage was carried out through single factor experiments and orthogonal experiment design. The best formula and fermentation condition of the beverage were as follows: 1:2 volume ratio of kelp juice to apple juice, 3% sucrose addition, 8% Lactobacillus plantarum inoculation, fermentation time 24 hours and temperature 32 ℃. The result of antioxidant activity assay showed that the inhibition rate of DPPH free radical, ABTS⁺ free radical and total antioxidant capacity of fermented kelp-apple juice beverage were 73.20%, 36.05% and 0.774, respectively. On the whole, both kelp juice and apple juice showed certain antioxidant capacity. Fermentation improved the antioxidant capacity of kelp juice, but reduced the antioxidant capacity of apple juice. However, the fermentation of sole kelp juice had poor taste and flavor. The addition of apple juice and lactic acid produced by fermentation masked the fishy smell of kelp to a certain extent. On the other hand, the addition of kelp juice prevented decline of antioxidant capacity of sole apple juice fermentation. Fermentation of two juices could significantly improve the DPPH free radical scavenging capacity of beverage. This study solved the problem of fishy smell of kelp beverage, explored the antioxidant activity of kelp-apple juice beverage fermentation system, and would provide theoretical basis and practical guidance for the development of kelp-fruit juice beverage.
- Saccharina japonica,
- apple juice,
- Lactobacillus plantarum,
- fermentation process,
- antioxidant activity

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References

[1]	宋武林. 海带的主要功能及加工利用研究现状[J]. 渔业研究,2016,38(1):81−86. [SONG W L. Research on the primary functions and utilization of Laminaria japonica[J]. Journal of Fisheries Research,2016,38(1):81−86.
[2]	王文亮, 王守经, 宋康, 等. 中国海带资源的功能及其开发利用研究[J]. 农业工程技术(农产品加工业),2008(4):40−41. [WANG W L, WANG S J, SONG K, et al. Research on the function and exploitation of the Laminaria japonica resource[J]. Agriculture Engineering Technology (Agricultural Product Processing Industry),2008(4):40−41.
[3]	康慧宇, 杨正勇, 张智一. 我国海藻产业发展研究[J]. 海洋开发与管理,2018,35(6):11−14. [KANG H Y, YANG Z Y, ZHANG Z Y. Development of seaweed industry in China[J]. Ocean Development and Management,2018,35(6):11−14. doi: 10.3969/j.issn.1005-9857.2018.06.003
[4]	郭峰君, 易灵红, 容英霖, 等. 海带加工现状研究[J]. 河北渔业,2020(4):45−48. [GUO F J, YI L H, RONG Y L, et al. Research on the status of Laminaria japonica[J]. Hebei Fisheries,2020(4):45−48. doi: 10.3969/j.issn.1004-6755.2020.04.014
[5]	王宁. 发酵果蔬汁饮料发展现状及趋势分析[J]. 农业科技与装备,2017(5):75−76. [WANG N. Analysis on development status and trend of fermented fruit and vegetable juice beverage[J]. Agricultural Science & Technology and Equipment,2017(5):75−76.
[6]	陈树俊, 郑婕. 复合果蔬发酵汁胃肠抗氧化活性及有机酸的变化[J/OL]. 食品科学: 1−11[2020-11-11]. http://kns.cnki.net/kcms/detail/11.2206.TS.20200722.1515.180.html. CHEN S J, ZHENG J. Changes on gastrointestinal antioxidant activity and organic acid of compound fruit and vegetable fermentation juice[J/OL]. Food Science: 1−11[2020-11-11]. http://kns.cnki.net/kcms/detail/11.2206.TS.20200722.1515.180.html.
[7]	张鑫, 刘光鹏, 宋烨, 等. 益生菌在发酵果蔬汁中的研究进展[J]. 中国果菜,2020,40(9):40−45. [ZHANG X, LIU G P, SONG Y, et al. Research progress on probiotics in fermented fruit and vegetable juice[J]. China Fruit & Vegetable,2020,40(9):40−45.
[8]	史瑞雨, 史瑞雪. 乳酸菌发酵果蔬汁研究进展[J]. 现代食品,2019(8):49−54. [SHI R Y, SHI R X. Progress of Lactobacillus fermentation of fruit and vegetable juice[J]. Modern Food,2019(8):49−54.
[9]	蔡建秀, 林微. 海带-竹叶复合饮料的研制[J]. 食品工业,2015,36(7):16−20. [CAI J X, LIN W. Development of the kelp with bamboo compound beverage[J]. The Food Industry,2015,36(7):16−20.
[10]	高代代, 冉军舰, 赵功玲, 等. 乳酸菌发酵苹果汁工艺研究[J]. 河南科技学院学报(自然科学版),2021,49(3):31−38. [GAO D D, RAN J J, ZHAO G L, et al. Study on the technology of apple juice fermented by lactic acid bacteria[J]. Journal of Henan Institute of Science and Technology (Natural Science Edition),2021,49(3):31−38.
[11]	KRIENGSAK T, UNAROJ B, KEVIN C, et al. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts[J]. Journal of Food Composition and Analysis,2006,19(6):669−675.
[12]	XIE J, SCHAICH K M. Re-evaluation of the 2, 2-diphenyl-1-picrylhydrazyl free radical (DPPH) assay for antioxidant activity[J]. Journal of Agricultural and Food Chemistry,2014,62(19):4251−4260. doi: 10.1021/jf500180u
[13]	MARINO B A, ANTONIO C, MANUEL A. The hydrophilic and lipophilic contribution to total antioxidant activity[J]. Food Chemistry,2001,73(2):239−244. doi: 10.1016/S0308-8146(00)00324-1
[14]	AUNG T, EUN J B. Production and characterization of a novel beverage from laver (Porphyra dentata) through fermentation with kombucha consortium[J]. Food Chemistry,2021,350:129274−129274. doi: 10.1016/j.foodchem.2021.129274
[15]	ZHAO Y, WANG C, LU W, et al. Evolution of physicochemical and antioxidant properties of whey protein isolate during fibrillization process[J]. Food Chemistry,2021,357:129751−129751. doi: 10.1016/j.foodchem.2021.129751
[16]	安兴娟, 张瑶, 姬阿美, 等. 植物乳杆菌发酵枸杞胡萝卜汁工艺的优化[J]. 天津科技大学学报,2016,31(3):20−24. [AN X J, ZHANG Y, JI A M, et al. Optimizing the preparation techniques for Lactobacillus plantarum-fermented medlar-carrot juice[J]. Journal of Tianjin University of Science & Technology,2016,31(3):20−24.
[17]	李鹏程, 刘青, 项耀东, 等. 复合乳酸菌发酵西瓜-番茄果蔬汁的工艺优化[J]. 现代食品科技,2020,36(6):249−255,67. [LI P C, LIU Q, XIANG Y D, et al. Optimization of fermented watermelon-tomato juice by compound lactic acid bacteria[J]. Modern Food Science and Technology,2020,36(6):249−255,67.
[18]	赵敏, 王瑜, 李立郎, 等. 天麻酵素化过程中风味物质及抗氧化活性动态变化[J/OL]. 食品与发酵工业: 1−9[2021-05-15]. http://kns.cnki.net/kcms/detail/11.1802.TS.20210510.1821.009.html. ZHAO M, WANG Y, LI L L, et al. Dynamic study on flavor components and activity in the fermentation of Rhizoma gastrodiae[J/OL]. Food and Fermentation Industries: 1−9[2021-05-15]. http://kns.cnki.net/kcms/detail/11.1802.TS.20210510.1821.009.html.
[19]	张珺, 喻婷, 许浩翔, 等. 乳酸菌发酵刺梨-猴头菇饮料的工艺优化[J]. 现代食品科技,2020,36(11):202−211,195. [ZHANG J, YU T, XU H X, et al. Process optimization of Rosa roxburghii Tratt - Hericium erinaceus beverage fermented by lactic acid bacteria[J]. Modern Food Science and Technology,2020,36(11):202−211,195.
[20]	戚向阳, 陈福生, 陈维军, 等. 苹果多酚抑菌作用的研究[J]. 食品科学,2003(5):33−36. [QI X Y, CHEN F S, CHEN W J, et al. Study on antibacterial effect of apple-polyphenol extracts[J]. Food Science,2003(5):33−36. doi: 10.3321/j.issn:1002-6630.2003.05.004
[21]	叶盼, 吴慧, 王德纯, 等. 发酵苹果汁的抗氧化性能变化[J]. 食品与发酵工业,2016,42(4):114−119. [YE P, WU H, WANG D C, et al. Changes on antioxidant activity of fermented apple juice[J]. Food and Fermentation Industries,2016,42(4):114−119.
[22]	孙海峰, 吕海涛, 周莎莎, 等. HPLC法测定苹果浓缩汁中的多酚类物质[J]. 食品科学,2008,29(4):314−317. [SUN H F, LV H T, ZHOU S S, et al. Simultaneous determination of phenolic compounds in apple juice concentrate by high performance liquid chromatography[J]. Food Science,2008,29(4):314−317. doi: 10.3321/j.issn:1002-6630.2008.04.067
[23]	RODRIGUEZ H, CURIEL J A, LANDETE J M, et al. Food phenolics and lactic acid bacteria[J]. International Journal of Food Microbiology,2009,132(2):79−90.
[24]	郑若宇. 益生菌发酵苹果汁工艺优化及发酵过程中风味物质和功能性成分的变化[D]. 沈阳: 沈阳农业大学, 2020. ZHENG R Y. Optimization of fermentation technology of apple juice by probiotics and changes of flavor substances and functional components during fermentation[D]. Shenyang: Shenyang Agricultural University, 2020.
[25]	BLACK W A P, DEWAR E T, WOODWARD F N. Manufacture of algal chemicals. IV—Laboratory-scale isolation of fucoidan from brown marine algae[J]. Journal of the Science of Food & Agriculture,2010,3(3):122−129.
[26]	CATHERINE R E, NICHOLAS M, GEORGE P. Antioxidant properties of phenolic compounds[J]. Trends in Plant Science,1997,2(4):152−159. doi: 10.1016/S1360-1385(97)01018-2
[27]	PAOLA A T R, HUGO E S, JESÚS I M Á, et al. Bioprospecting potential of kelp (Laminariales, Phaeophyceae) from Baja California Peninsula: Phenolic content, antioxidant properties, anti-inflammatory, and cell viability[J]. Journal of Applied Phycology,2019,31(5):3115−3129. doi: 10.1007/s10811-019-01781-1
[28]	李长见. 抗氧化小米发酵饮料的研制[J]. 饮料工业,2017,20(6):27−30. [LI C J. Development of antioxidant millet fermented beverage[J]. Beverage Industry,2017,20(6):27−30. doi: 10.3969/j.issn.1007-7871.2017.06.010
[29]	马泽刚, 杨新春, 辛超, 等. 绞股蓝饮料的研制及其抗氧化性[J]. 食品工业,2020,41(11):1−6. [MA Z G, YANG X C, XIN C, et al. Preparation of Gynostemma pentaphyllum beverage and its antioxidant activity[J]. Food Industry,2020,41(11):1−6.