Effects of the Extract of <i>Gnaphalium affine</i> on the Oxidation and Structure of Myofibrillar Protein Induced by ·OH

XIONG Jie; BO Zhaoying; CHANG Haijun; ZHOU Wenbin; ZHU Jianfei

doi:10.13386/j.issn1002-0306.2021050021

Volume 43 Issue 3

Jan. 2022

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Science and Technology of Food Industry > 2022 > 43(3): 72-80. > DOI: 10.13386/j.issn1002-0306.2021050021

XIONG Jie, BO Zhaoying, CHANG Haijun, et al. Effects of the Extract of Gnaphalium affine on the Oxidation and Structure of Myofibrillar Protein Induced by ·OH[J]. Science and Technology of Food Industry, 2022, 43(3): 72−80. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021050021.

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Effects of the Extract of Gnaphalium affine on the Oxidation and Structure of Myofibrillar Protein Induced by ·OH

Chongqing Engineering Research Center for Processing, Storage and Transportation of Characterized Agro-Products, College of Environment and Resources, Chongqing Technology and Business University, Chongqing 400067, China

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Received Date: May 07, 2021
Available Online: December 19, 2021

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Abstract

The effects of tea polyphenols (TP) and Gnaphalium affine extract (GAE) at different concentrations (0, 1.2, 6, 30 mg/g protein) on the oxidation and structural changes of porcine myofibrillar proteins (MPs) were studied using Fenton oxidation system to simulate the actual meat oxidation environment. The results showed that GAE had a strong ability to scavenging free radicals, which could significantly inhibit the formation of carbonyl group and promote the loss of sulfhydryl group (P<0.05), as did TP. Especially under high concentration, TP and GAE inhibited 36.89% and 38.93% carbonyl formation, and promoted 28.89% and 27.40% sulfhydryl loss, respectively. In addition, TP and GAE both had strong quenching effect on the endogenous fluorescence of MPs, promoted the further expansion of the protein structure, aggravated the loss of α-helical conformation caused by oxidation, exposed more active groups, and significantly increased the hydrophobicity on the protein surface and protein insolubility (P<0.05). The higher the concentration of TP and GAE, the greater the effect on the oxidation and structural changes of MPs, but GAE was not as effective as TP in regulating the oxidation of MPs and its interaction with proteins.
- Gnaphalium affine extract (GAE),
- myofibrillar proteins (MPs),
- oxidation,
- interaction,
- physical and chemical properties

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