Acetylcholinesterase Inhibition Effect of Flavonoids from <i>Flemigia philippinensis</i>

WANG Yan; YUAN Fengjuan; ZHANG Meng; YU Lu; LIU Wenyu; WU Hongyan; GAO Jianwei; WANG Tuoyi

doi:10.13386/j.issn1002-0306.2021040243

Volume 42 Issue 13

Jun. 2021

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Science and Technology of Food Industry > 2021 > 42(13): 118-124. > DOI: 10.13386/j.issn1002-0306.2021040243

WANG Yan, YUAN Fengjuan, ZHANG Meng, et al. Acetylcholinesterase Inhibition Effect of Flavonoids from Flemigia philippinensis [J]. Science and Technology of Food Industry, 2021, 42(13): 118−124. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021040243.

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Acetylcholinesterase Inhibition Effect of Flavonoids from Flemigia philippinensis

College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China

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Received Date: April 25, 2021
Available Online: May 20, 2021

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Abstract

Abstract

In order to study the inhibitory effect of the active ingredients of the Flemigia philippinensis roots on acetylcholinesterase (AChE), the roots of the Flemigia philippinensis were used as raw materials, and different solvent systems were applied to fractionate and extract flavonoids, and then further separate and purify them. Reversible inhibition kinetics method were applied to study the inhibitory effect of different solvent extracts and four flavonoids on AChE and related constants. The results showed that the different solvent extracts and the four favonoids (Genistein, Philippin C, Philippin D and Flemiphilippinone A) from the roots of Flemigia philippinensis had a strong inhibitory effect on AChE. The inhibition type was mixed type inhibition, and the half inhibition concentration (IC₅₀) was (57.86±0.67)~(127.28±1.22) µmol/L, the inhibition constant K_i was (54.8±0.43)~(120.58±1.35) µmol/L. The research results could provide a theoretical basis and experimental basis for the discovery of high-efficiency acetylcholinesterase inhibitor lead compounds and the development and comprehensive utilization of Flemigia philippinensis related foods.
- Flemigia philippinensis,
- acetylcholinesterase,
- inhibitor,
- flavonoids,
- inhibition kinetics

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