Optimization of Fermentation Medium Formula and Fermentation Conditions of <i>Tremella fuciformis</i> Spore Polysaccharide and Its Scale-up Fermentation Experiment

TANG Rui; LIN Junfang; GUO Liqiong; YE Zhiwei; ZHENG Qianwang

doi:10.13386/j.issn1002-0306.2021030083

Volume 42 Issue 20

Oct. 2021

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Science and Technology of Food Industry > 2021 > 42(20): 173-182. > DOI: 10.13386/j.issn1002-0306.2021030083

TANG Rui, LIN Junfang, GUO Liqiong, et al. Optimization of Fermentation Medium Formula and Fermentation Conditions of Tremella fuciformis Spore Polysaccharide and Its Scale-up Fermentation Experiment[J]. Science and Technology of Food Industry, 2021, 42(20): 173−182. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030083.

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Optimization of Fermentation Medium Formula and Fermentation Conditions of Tremella fuciformis Spore Polysaccharide and Its Scale-up Fermentation Experiment

TANG Rui^{1, 2,},
LIN Junfang^{1, 2},
GUO Liqiong^{1, 2},
YE Zhiwei^{1, 2},
ZHENG Qianwang^{1, 2, ,}

1.
South China Agricultural University, Guangzhou 510640, China
2.
Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou 510642, China

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Received Date: March 08, 2021
Available Online: August 11, 2021

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Abstract

Abstract

In order to develop a submerged fermentation medium for high-yield extracellular polysaccharides (EPS) of Tremella fuciformis spore, to improve its fermentation yield and achieve large-scale production applications, this study used a single factor method to explore the effects of different sources of carbon and nitrogen sources and nutrient elements on the yield of Tremella polysaccharides. Through the Plackett-Burman and Box-Behnken response surface design to obtain the high-yield EPS fermentation medium and fermentation conditions, and verified the optimization results in a 50 L fermentation tank. The results showed that the fermentation medium of Tremella spores with high production of extracellular polysaccharides was: Glucose 35 g/L, NaCl 0.6 g/L, compound nitrogen source (yeast extracts:corn steep dry powder=1:1) 3.6 g/L, MgSO₄ 0.5 g/L, KH₂PO₄ 1 g/L; Optimal fermentation conditions were: 27 ℃, pH5, fermentation for 6 days, inoculation amount 3%(v/v) and shaker speed 150 r/min. After optimization, the yield of extracellular polysaccharide from Tremella spore was 214.45 mg/100 mL, with 8.56 times of that before optimization. In the 50 L fermentation tank, the polysaccharide yield was 258.78 mg/100 mL with 21.03% higher than the shake flask experiment. This optimization of the fermentation process significantly promoted the production of extracellular polysaccharides from Tremella spore, which would provide a theoretical support for its large-scale industrial production.

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