Study on Biological Activity of Different Solvent Extracts from Ophiolossum vulgatum L. in Vitro

ZENG Yongfang; LIAO Huazhen; XU Haitang; WANG Cong; WEI Yichun

doi:10.13386/j.issn1002-0306.2021020167

Volume 42 Issue 22

Nov. 2021

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Science and Technology of Food Industry > 2021 > 42(22): 363-368. > DOI: 10.13386/j.issn1002-0306.2021020167

ZENG Yongfang, LIAO Huazhen, XU Haitang, et al. Study on Biological Activity of Different Solvent Extracts from Ophiolossum vulgatum L. in Vitro[J]. Science and Technology of Food Industry, 2021, 42(22): 363−368. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020167.

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Study on Biological Activity of Different Solvent Extracts from Ophiolossum vulgatum L. in Vitro

1.
Guigang Centers for Disease Control and Prevention, Guigang 537100, China
2.
School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China

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Received Date: February 22, 2021
Available Online: August 30, 2021

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Abstract

Abstract

The antioxidant, antibacterial and antiproliferative activities of different solvents (chloroform, ethyl acetate, n-butanol, ethanol) extracts from Ophiolossum vulgatum L. were compared. The antioxidant activity of four extracts was evaluated by determining the removal effect of three free radicals, with ascorbic acid used as control. The antimicrobial activity of the four extracts against several common pathogenic bacteria was determined by microdilution assay. The antiproliferative activities on SKOV3 cells were detected by MTT assay. The results showed that all the extracts had certain antioxidant, antibacterial and antiproliferative activities, especially n-butanol extract exhibited the strongest activity among them. The scavenging effect of n-butanol extract was the best for DPPH free radicals, with IC₅₀ value of 0.114 mg/mL, and it had obvious inhibitory effect on beta hemolytic Streptococcus, Pseudomonas aeruginosa and Monilia albican, with MIC value of 0.313 mg/mL, and had the strongest inhibitory effect on SKOV3 ovarian cancer cells, with IC₅₀value of 114.5 μg/mL. The n-butanol extract from Ophiolossum vulgatum L. showed the potential to be further studied and used in the fields of drugs and functional foods.
- Ophiolossumvulgatum L.,
- different solvent extracts,
- antioxidant activity,
- antibacterial activity,
- antiproliferative activity

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