Determination of Sulfonamides in Beef by One Step QuEChERS Method Combined with High Performance Liquid Chromatography-Tandem Mass Spectrometry

XIE Yujie; LI Tiemei; NIU Xiangtao; ZHANG Yanxia; HU Bin; WU Xingqiang; FAN Chunlin; CHEN Hui

doi:10.13386/j.issn1002-0306.2021010156

Volume 42 Issue 20

Oct. 2021

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Science and Technology of Food Industry > 2021 > 42(20): 268-278. > DOI: 10.13386/j.issn1002-0306.2021010156

XIE Yujie, LI Tiemei, NIU Xiangtao, et al. Determination of Sulfonamides in Beef by One Step QuEChERS Method Combined with High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(20): 268−278. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010156.

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Determination of Sulfonamides in Beef by One Step QuEChERS Method Combined with High Performance Liquid Chromatography-Tandem Mass Spectrometry

1.
Chinese Academy of Inspection and Quarantine, Beijing 100176, China
2.
Shandong Institute for Food and Drug Control, Jinan 250101, China

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Received Date: January 19, 2021
Available Online: August 18, 2021

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Abstract

The method for simultaneous determination of 25 sulfonamides residues in beef was established by one-step automatic extraction and purification technology of QuEChERS combined with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The sample was extracted with 1% acetonitrile acetate, C₁₈, PSA and anhydrous magnesium sulfate were used as purification adsorbents, and the one-step QuEChERS automatic pretreatment equipment was used for extraction, purification and centrifugation. The analytes were separated by a reversed phase C₁₈ column and eluted with 0.2% formic acid methanol and 0.2% formic acid water (containing 2 mmol/L ammonium acetate). The beef sample was analyzed under the multiple reaction monitoring (MRM) mode with positive electrospray ionization (ESI+) and quantify by external standard. The results showed that good linearities were obtained for the 25 sulfonamides in 0.1~20 μg/L with the linear correlation coefficients more than 0.990. The limits of detection (LOD) and the limits of quantification (LOQ) for sulfonamides were 0.1~3 μg/kg and 1~10 μg/kg, respectively. The recoveries at three levels were in the range of 70.14%~116.93%. The repeatability expressed as relative standard deviations (RSD) was ranged from 0.95% to 13.74%(n=5). The method was simple, rapid, accurate and reliable, and could be used to detect the residues of sulfonamides in beef.
- one-step QuEChERS,
- beef,
- sulfonamides,
- high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)

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