Determination of Five Carotenoids in Eggs and Chicken by HPLC

LAN Tao; YAN Na; PEI Dong; LIU Jiawen; YU Congcong; CHU Qiao; LI Duxin

doi:10.13386/j.issn1002-0306.2021010109

Volume 42 Issue 20

Oct. 2021

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Science and Technology of Food Industry > 2021 > 42(20): 261-267. > DOI: 10.13386/j.issn1002-0306.2021010109

LAN Tao, YAN Na, PEI Dong, et al. Determination of Five Carotenoids in Eggs and Chicken by HPLC[J]. Science and Technology of Food Industry, 2021, 42(20): 261−267. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010109.

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Determination of Five Carotenoids in Eggs and Chicken by HPLC

LAN Tao^1,,
YAN Na^{1, 2},
PEI Dong³,
LIU Jiawen^{1, 2},
YU Congcong¹,
CHU Qiao¹,
LI Duxin^2, ,

1.
China National Institute of Standardization, Beijing 100191, China
2.
College of Pharmaceutical Sciences, Soochow University, Suzhou 215006, China
3.
Qingdao Center of Resource Chemistry & New Materials, Qingdao 266000, China

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Received Date: January 14, 2021
Available Online: August 03, 2021

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Abstract

Abstract

A HPLC method for simultaneous determination of astaxanthin, lutein, cantharidin, zeaxanthin and β-carotene in eggs and chicken was developed. The results showed a good linear relationship in the range of 0.5~100 μg/mL for five carotenes, and the coefficients of determination were all above 0.996. The limits of detection of the method were between 0.10 and 0.20 mg/kg, with the limits of quantitation were between 0.33 and 0.66 mg/kg. At the spiked levels of 1, 10 and 20 mg/kg, the recoveries of eggs and chicken samples were 71.6%~112.9% and 88.6%~119.7%, respectively, with RSD≤7.2%. The method is rapid, simple, accurate and reproducible. It is suitable for the detection of five kinds of carotenoids in complex matrix samples such as eggs and chicken. It is of great significance for the market supervision of eggs and chicken.
- high performance liquid chromatography (HPLC),
- egg,
- chicken,
- carotenoids

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References (31)

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