Uncertainty Evaluation of Determination of Quinolones in Flatfish by High Performance Liquid Chromatography-Tandem Mass Spectrometry

FAN Guangyu; TANG Xiu; ZHANG Lin; LI Peiting; CHEN Zhenling

doi:10.13386/j.issn1002-0306.2021010077

Volume 42 Issue 20

Oct. 2021

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Science and Technology of Food Industry > 2021 > 42(20): 248-254. > DOI: 10.13386/j.issn1002-0306.2021010077

FAN Guangyu, TANG Xiu, ZHANG Lin, et al. Uncertainty Evaluation of Determination of Quinolones in Flatfish by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(20): 248−254. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010077.

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Uncertainty Evaluation of Determination of Quinolones in Flatfish by High Performance Liquid Chromatography-Tandem Mass Spectrometry

1.
Lianyungang Customs, Lianyungang 222042, China
2.
Yancheng Customs, Yancheng 224002, China
3.
Guizhou Institute of Technology, Guiyang 550003, China

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Received Date: January 12, 2021
Available Online: August 18, 2021

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Abstract

Abstract

A method for the determination of ofloxacin, pefloxacin, norfloxacin and lomefloxacin in flatfish by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established. Accoding to SN/T 1751.2-2007 Determination of Multi-residues of Quinolones in Food of Animal Origin for Import and Export-Part 2: LC-MS/MS Method, a mathematical model for uncertainty evaluation was established and the sources of uncertainty were analysed. The expanded uncertainty was 1.04, 1.09, 1.34 and 0.99 μg/kg(k=2) for ofloxacin, pefloxacin, norfloxacin and lomefloxacin of contents of 9.26, 10.08, 9.73 and 9.57 μg/kg in flatfish, respectively. As a result, the fitting of the standard curve, the high performance liquid chromatography-tandem mass spectrometry and the preparation of standard solution were the main sources of uncertainty.
- high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- flatfish,
- quinolones,
- uncertainty,
- evaluation

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References

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