Aperient Effect of <i>Dendrobium nobile</i> Lindl Extract on Constipation Model Mice

WANG Weiwei; FAN Hongjie; YANG Wenhong; XIA Kaiyan; LI Huagui; LI Xiaoli; WANG Shiman; ZHANG Yan

doi:10.13386/j.issn1002-0306.2020120264

Volume 42 Issue 20

Oct. 2021

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Science and Technology of Food Industry > 2021 > 42(20): 342-347. > DOI: 10.13386/j.issn1002-0306.2020120264

WANG Weiwei, FAN Hongjie, YANG Wenhong, et al. Aperient Effect of Dendrobium nobile Lindl Extract on Constipation Model Mice[J]. Science and Technology of Food Industry, 2021, 42(20): 342−347. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120264.

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Aperient Effect of Dendrobium nobile Lindl Extract on Constipation Model Mice

1.
College of Basic Medical Sciences, Zunyi Medical University, Zunyi 563000, China
2.
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
3.
Clinical College, Zunyi Medical University, Zunyi 563000, China
4.
College of Pharmacy, Zunyi Medical University, Zunyi 563000, China

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Received Date: December 29, 2020
Available Online: August 15, 2021

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Abstract

Abstract

Objective: To investigate the effect of Dendrobium nobile Lindl extract on improvement of constipation and intestinal Lactobacillus diversity of mice. Methods: The mice with constipation induced by in compound diphenoxylate were given the high-dose (300 mg/kg), middle-dose (100 mg/kg), low-dose (30 mg/kg) of Dendrobium nobile Lindl extract, respectively. The defecation function and intestinal propulsive rate in mice were observed. Using Lactobacillus-specific primer and the microbial genomic DNA extracted from the fecal samples, the ARDRA analysis of Lactobacillus diversity was accomplished after PCR amplification and enzyme digestion. Results: After intragastric administration, the defecation time of the first black stool decreased significantly (P<0.01). The number of fecal particles was increased, and the fecal water content increased significantly (P<0.01). The intestinal propulsive rate increased significantly in the middle-dose group and the high-dose group (P<0.05). The diversity of Lactobacillus increased after intragastric administration by Dendrobium nobile Lindl extract, and the evenness of Lactobacillus in the intestine was more stable in the high-dose group. Conclusion: It showed that Dendrobium nobile extract had an improvement effect on the symptoms of constipation by increasing the fecal water content, promoting the intestinal peristalsis and regulating the diversity of intestinal Lactobacillus in mice.

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References

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