ZHANG Yunhong, PENG Yunpin, LU Chunxia, et al. Study of Aptamer-Based Lateral Flow Strip and Its Application for Qualitative Detection of Staphylococcus aureus [J]. Science and Technology of Food Industry, 2021, 42(19): 291−297. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120169.
Citation: ZHANG Yunhong, PENG Yunpin, LU Chunxia, et al. Study of Aptamer-Based Lateral Flow Strip and Its Application for Qualitative Detection of Staphylococcus aureus [J]. Science and Technology of Food Industry, 2021, 42(19): 291−297. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120169.

Study of Aptamer-Based Lateral Flow Strip and Its Application for Qualitative Detection of Staphylococcus aureus

More Information
  • Received Date: December 17, 2020
  • Available Online: July 28, 2021
  • In this experiment, Staphylococcus aureus was selected as the model target, and a pair of aptamers were used as the recognition molecules. An aptamer-based lateral flow test strip (ABLF) was prepared for the qualitative detection of Staphylococcus aureus based on the sandwich-type model and principle of lateral flow chromato gram. Several crucial parameters were investigated, including the NaCl concentrations, aptamer concentrations, the loaded amounts of gold nanoparticle-aptamer conjugates and capture probe to obtain the best preparation conditions for ABLF. The sensitivity and specificity of the ABLF were tested under optimized conditions. Finally, the ABLF approach was applied for qualitative detection of Staphylococcus aureu in 116 food samples, and was validated using sandard method (GB 4789.10-2016). The results showed that the optimal conditions for preparing ABLF were as follows: NaCl concentration 80 mmol/L, aptamer concentration 1 µmol/L, dilution volume ratio of gold nanoparticles-aptamer 1:2, capture probe concentration 25 μmol/L. Under the optimal experimental conditions, the visual limit of detection of the strip for Staphylococcus aureus were 2×103 CFU/mL. The assay can be completed within 5 min, and no significant cross-reactivity with other foodborne pathogens such as Salmonella typhimurium, Listeria monocytogenes, Escherichia coli, Bntorobater sakazakii, Shigella dysenteriae were observed. The test results obtained by the proposed method were found to be in consistency with those obtained from GB sandard method. The method developed was simple, rapid, accurate and inexpensive, which could be a potential screening tool for the qualitative detection of Staphylococcus aureus in food samples.
  • [1]
    Pinchuk I V, Beswick E J, Reyes V E. Staphylococcal enterotoxins[J]. Toxiiis (Basel),2010,2(8):2177−2197. doi: 10.3390/toxins2082177
    [2]
    Scallan E, Hoekstra R M, Angulo F J. Foodborne illness acquired in the United States-major pathogens[J]. Emerging Infectious Disease,2011,17(1):7−15. doi: 10.3201/eid1701.P11101
    [3]
    Liu J, Bai L, Li W, et al. Trends of food borne diseases in China: Lessons from laboratory-based surveillance since 2011[J]. Frontiers of Medicine,2018,12(1):48−57. doi: 10.1007/s11684-017-0608-6
    [4]
    黄欣悦, 陈娟, 马欣玥. 食品中金黄色葡萄球菌定量风险评估的研究进展[J]. 食品工业科技,2020. [Huang Xinyue, Chen Juan, Ma Xinyue. Research progress of quantitative risk assessment of Staphylococcus aureus in food[J]. Science and Technology of Food Industry,2020. doi: 10.13386/j.issn1002-0306.2020080234
    [5]
    GB 29921-2013食品安全国家标准食品中致病菌限量[S]. 北京: 中国标准出版社, 2013.

    GB 29921-2013 National food safety standard limit of pathogenic bacteria in food[S]. Beijing: China Standards Press, 2013.
    [6]
    GB 4789.10-2016食品安全国家标准食品微生物学检验金黄色葡萄球菌检验[S]. 北京: 中国标准出版社, 2016.

    GB 4789.10-2016 National food safety standard food microbiological inspection staphylococcus aureus inspection[S]. Beijing: China Standards Press, 2016.
    [7]
    邓奕, 许迪莘, 赵琳娜, 等. 免疫磁球捕获PCR检测牛奶中金黄色葡萄球菌的研究[J]. 食品工业科技,2015,36(7):304−307, 313. [Deng Yi, Xu Dixin, Zhao Linna, et al. Immunomagnetic separation combined with polymerase chain reaction for the detection of Staphylococcus aureus in milk[J]. Science and Technology of Food Industry,2015,36(7):304−307, 313.
    [8]
    郭建平, 万佳佳, 陆兆新, 等. 基于可视化环介导等温扩增技术快速检测金黄色葡萄球菌[J]. 食品科学,2019,40(20):325−331. [Guo Jianping, Wan Jiajia, Lu Zhaoxin, et al. Rapid visual detection of Staphylococcus aureus using loop-mediated is othermal amplification[J]. Food Science,2019,40(20):325−331. doi: 10.7506/spkx1002-6630-20180830-332
    [9]
    Park S J, Kim Y K. Enzyme-linked immunosorbent assay strip sensor for rapid detection of Staphylococcus aureus[J]. Applied Chemistry for Engineering,2011,22(5):522−525.
    [10]
    Nouri A, Ahari H, Shahbazzadeh D. Designing a direct ELISA kit for the detection of Staphylococcus aureus enterotox in a in raw milk samples[J]. International journal of Biological Macromolecules,2018,107:1732−1737. doi: 10.1016/j.ijbiomac.2017.10.052
    [11]
    Li J B, Yang Y Q, Wang J H, et al. Resonance rayleigh scattering detection of the epidermal growth factor receptor based on an aptamer-functionalized gold-nanoparticle probe[J]. Analytical Methods,2018,10:2910−2916. doi: 10.1039/C8AY00860D
    [12]
    Lu C X, Liu C B, Shi G Q. Colorimetric enzyme-linked aptamer assay utilizing hybridization chain reaction for determination of bovine pregnancy-associated glycoproteins[J]. Microchimica Acta,2020,187:316−324. doi: 10.1007/s00604-020-04301-y
    [13]
    袁敏, 钱世权, 曹慧, 等. 一种超灵敏检测镉离子的核酸适配体电化学传感器[J]. 分析化学,2020,48(12):1701−1708. [Yuan Min, Qian Shiquan, Cao Hui, et al. An ultra-sensitive electrochemical aptasensor for detection of Cd2+[J]. Chinese Journal of Analytical Chemistry,2020,48(12):1701−1708.
    [14]
    王红旗, 张玲, 刘冬梅, 等. 小分子靶标核酸适配体研究进展[J]. 食品与生物技术学,2015,34(8):790−798. [Wang Hongqi, Zhang Ling, Liu Dongmei, et al. Research progress of aptamer for small molecule target[J]. Journal of Food Science and Biotechnology,2015,34(8):790−798.
    [15]
    Wu Q Y, Wu L, Wang Y Z, et al. Evolution of DNA aptamers for malignant brain tumor gliosarcoma cell recognition and clinical tissue imaging[J]. Biosensors and Bioelectronics,2016,80:1−8. doi: 10.1016/j.bios.2016.01.031
    [16]
    Xu X M, MaA X Y, Wand H T, et al. Aptamer based SERS detection of Salmonella typhimurium using DNA-assembled gold nanodimers[J]. Microchimica Acta,2018,185:325−332. doi: 10.1007/s00604-018-2852-0
    [17]
    王周平, 张微潇. 适配体及其研究进展[J]. 食品与生物技术学报,2013,32(9):897−906. [Wang Zhouping, Zhang Wei Xiao. Aptamer and related progress[J]. Journal of Food Science and Biotechnology,2013,32(9):897−906.
    [18]
    Chang Y C, Yang C Y, Sun R L, et al. Rapid single cell detection of Staphylococcus aureus by aptamer-conjugated gold nanoparticles[J]. Scientific Reports,2013,3:1863−1869. doi: 10.1038/srep01863
    [19]
    Cao X X, Li S H, Chen L C, et al. Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus[J]. Nucleic Acids Research. 2009, 37: 4621-4628.
    [20]
    Duan N, Wu S J, Zhu C Q, et al. Dual-color upconversion fluorescence and aptamer-functionalized magnetic nanoparticles-based bioassay for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureus[J]. Anal Chim Acta,2012,723:1−6. doi: 10.1016/j.aca.2012.02.011
    [21]
    Abbaspour A, Norouz-Sarvestani F, Noori A, et al. Aptamer-conjugated silver nanoparticles for electrochemical dual-aptamer-based sandwich detection of Staphylococcus aureus[J]. Biosensors and Bioelectronics,2015,68:149−15. doi: 10.1016/j.bios.2014.12.040
    [22]
    龚频, 王思远, 陈雪峰, 等. 胶体金免疫层析试纸条技术及其在食品安全检测中的应用研究进展[J]. 食品工业科技,2019,13:358−364. [Gong Pin, Wang Siyuan, Chen Xuefeng, et al. Research progress of colloidal gold immunochromatographic test strip technology and its application in food safety testing[J]. Science and Technology of Food Industry,2019,13:358−364.
    [23]
    SB/T 10779-2012 动物肌肉中盐酸克伦特罗的快速筛查胶体金免疫层析法[S]. 2012.

    SB/T 10779-2012 Rapid screening of clenbuterol in animal muscles by colloidal gold immunochromatography[S]. 2012.
    [24]
    Liu J, Zeng J Y, TianY P, et al. Aptamer and functionalized nanoparticles-based strip biosensor for on-site detection of kanamycin in food samples[J]. Analyst,2017,143(1):182−189.
    [25]
    谢娟, 王延吉, 李艳廷, 等. 粒径可控纳米金的制备及表征[J]. 黄金,2008,7:3−6. [Xie Juan, Wang Yanji, Li yanting, et al. Synthesis and characterization of size-controllable gold nanoprticles[J]. Gold,2008,7:3−6. doi: 10.3969/j.issn.1001-1277.2008.07.002
    [26]
    卢春霞, 李红敏, 孙凤霞, 等. 基于适配体识别的侧向层析法快速检测单核细胞增生李斯特氏菌[J]. 食品与生物技术学报,2020,39(4):85−92. [Lu Chunxia, Li Hongmin, Sun Fengxia, et al. Aptamer-based lateral flow strip for rapid detection of Listeria monocytogenes[J]. Journal of Food Science and Biotechnology,2020,39(4):85−92. doi: 10.3969/j.issn.1673-1689.2020.04.012
    [27]
    Zhu C Y, Zhao M M, Yan Y F, et al. A sandwichdipstick assay for ATP detection based on split aptamer fragments[J]. Analytical and Bioanalytical Chemistry,2016,408(15):4151−4158. doi: 10.1007/s00216-016-9506-z
    [28]
    Shen G Y, Zhang S B, Hu X. Signal enhancement in a lateral flow immunoassay based on dual gold nanoparticle conjugates[J]. Clinical Biochemistry,2013,46:1734−1738. doi: 10.1016/j.clinbiochem.2013.08.010
    [29]
    Wu S J, Liu L H, Duan N, et al. An aptamer-based lateral flow test strip for rapid detection of zearalenone in corn samples[J]. Journal of Agricultural and Food Chemistry,2018,66(8):1949−1954. doi: 10.1021/acs.jafc.7b05326
    [30]
    Park H G, Paeng I R. Development of direct competitive enzyme-linked aptamer assay for determination of dopamine in serum[J]. Anal Chim Acta,2011,685(1):65−73. doi: 10.1016/j.aca.2010.11.010
  • Related Articles

    [1]YUN Ziguang, WEI Yong, ZHANG Jian, CUI Shuangshuang, LI Can, SUN Fengxia. Advances in the Rapid Detection of Foodborne Pathogens by Nucleic Acid Amplification Lateral Flow Assays[J]. Science and Technology of Food Industry, 2024, 45(5): 412-425. DOI: 10.13386/j.issn1002-0306.2023050216
    [2]YAN Lingzhi. Research Progress of Signal Amplification Strategies in Immunochromatographic Test Strip[J]. Science and Technology of Food Industry, 2022, 43(11): 34-44. DOI: 10.13386/j.issn1002-0306.2021120234
    [3]YAN Lingzhi. Research Progress of Lateral Flow Immunoassay in Food Safety[J]. Science and Technology of Food Industry, 2022, 43(4): 1-11. DOI: 10.13386/j.issn1002-0306.2021070031
    [4]CUI Yan, BAI Yalong, SHI Xianming. Progress on the Application of Aptamers in the Detection of Staphylococcus aureus[J]. Science and Technology of Food Industry, 2021, 42(21): 1-7. DOI: 10.13386/j.issn1002-0306.2021060130
    [5]YAN Lingzhi. Research Progress of Immunochromatographic Strip Technique in Food Safety[J]. Science and Technology of Food Industry, 2021, 42(11): 397-404. DOI: 10.13386/j.issn1002-0306.2020110079
    [6]SHA Zhi-cong, QI Mu-ge, JIA Zeng-yan, ZHANG Yan, SHENG Wei. Quantum Dot-Labeled Immunochromatographic Test Strip for Rapid Detection of Ochratoxin A in Cereals[J]. Science and Technology of Food Industry, 2019, 40(17): 191-195,190. DOI: 10.13386/j.issn1002-0306.2019.17.031
    [7]GONG Pin, WANG Si-yuan, CHEN Xue-feng, ZHANG Tao-tao, CHEN Fu-xin. Research Progress of Colloidal Gold Immunochromatographic Test Strip Technology and Its Application in Food Safety Testing[J]. Science and Technology of Food Industry, 2019, 40(13): 358-364. DOI: 10.13386/j.issn1002-0306.2019.13.060
    [8]ZHANG Qing-qing, LIU Hui-min, WU Ren-xie, YAN Qing-pi, CAO Bo, ZHAO Quan, ZHENG Jiang. Advances in application of aptamers in identification of marine microorganisms and marine toxins[J]. Science and Technology of Food Industry, 2018, 39(2): 320-324,330. DOI: 10.13386/j.issn1002-0306.2018.02.059
    [9]LI Sha, BAI Rui-ying, ZENG Dao-ping, WU Zhi-quan, SHEN Yu-dong, XU Zhen-lin, YANG Jin-yi. Investigation of colloidal gold immunochromatographic strip for quantitative determination of variety of β-agonists in swine urine[J]. Science and Technology of Food Industry, 2016, (20): 53-58. DOI: 10.13386/j.issn1002-0306.2016.20.002
  • Cited by

    Periodical cited type(14)

    1. 丁鑫华,谈坤,仪淑宁,曹杨,常宪辉,吕庆云,陈磊,丁文平,陈曦. 苦荞粉对米线品质及消化特性的影响. 中国粮油学报. 2024(12): 40-49 .
    2. 罗财伟,王茂飞,宁冬,王海彦,袁建敏. 不同类型和含水量玉米储存期间理化性质及糊化特性变化研究. 中国畜牧杂志. 2023(02): 207-214 .
    3. 孙剑,刘超,宋玉,曹磊,邵子晗,洪莹,陶澍,梁进,王仁和,吴广露. 功能性米线配方优化及品质分析研究. 中国粮油学报. 2023(03): 163-169 .
    4. 吴迪,葛飞,马红,汤晓智. 不同磨粉方式对青稞全粉理化特性的影响. 中国粮油学报. 2022(03): 59-67 .
    5. 赵云蛟,张巍毅,李乐铖,李闻,张民,刘锐,隋文杰,吴涛. 固体发酵麦麸对小麦粉和面条品质的影响. 食品科学. 2021(22): 45-52 .
    6. 朱慧雪,靳灿灿,温纪平. 挤压处理的小麦糊粉层粉添加量对小麦粉品质的影响. 河南工业大学学报(自然科学版). 2021(06): 39-47+56 .
    7. 戴春. 麦麸膳食纤维对控制中老年糖尿病患者血糖效果研究. 中国食物与营养. 2020(03): 57-60 .
    8. 刘悦,张国治,张康逸. 膳食纤维的功能特性及在饼干中的研究进展. 粮食加工. 2020(05): 64-69 .
    9. 马森,雷梦续,李力,王晓曦. 麦麸膳食纤维对面制品主成分的影响研究进展. 河南工业大学学报(自然科学版). 2020(05): 121-128 .
    10. 张玉爽,李景明,倪元颖,张兴,程继旺,薄佳钰,张晓旭. 朝鲜蓟超微粉对面团特性及馒头品质的影响. 食品研究与开发. 2020(24): 65-71 .
    11. 伍梦婷,郭娟娟,徐云强,罗凯凯,汪兰,吴文锦,熊光权,孙卫青. 木薯改性淀粉对鹅血凝胶特性的影响. 食品工业科技. 2019(06): 95-99+113 . 本站查看
    12. 李淦,姜小苓,吴晓军,李小军,茹振钢,王跃东,张双. 171份小麦种质面粉糊化特性及其与品质性状的相关性分析. 麦类作物学报. 2019(08): 973-978 .
    13. 殷欣,陈文,彭涛,曹莹,张小燕,路宏科. 小杂粮淀粉糊化及回生研究进展. 中国酿造. 2019(10): 26-30 .
    14. 龚川杰,姜萌艺,李美伦,何利. 不同发酵方式及发酵剂菌比对米发糕挥发性成分的影响. 食品研究与开发. 2019(24): 124-131 .

    Other cited types(16)

Catalog

    Article Metrics

    Article views (579) PDF downloads (43) Cited by(30)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return