Optimization of Enzymolysis Assisted Extraction Process of Dandelion Polysaccharide and Its Monosaccharide Composition Analysis

In this experiment, the content of dandelion polysaccharides was used as an indicator, and single factor combined with Box-Behnken response surface method was used to study the enzymolysis process, and the monosaccharide composition and scanning electron microscope of unenzymolysis and enzymolysis crude dandelion polysaccharides were determined. The results showed that the 10% wheatbran was added to the raw material of dandelion, and the content of dandelion polysaccharide could reach 218.21 mg/g after enzymolysis at 57.6 ℃, 1532 U/g of enzyme and 55% water content for 12.3 h. After enzymolysis, the content of dandelion polysaccharide increased by 78.09%, and the majormonosaccharides in dandelion polysaccharide were mannose, rhamnose, glucose, galactose, xylose, arabinose, fucose, and its molar ratio were 2.99:10.65:18:14.82:8.61:9.20:0.83, and the content of mannose, rhamnose, galactose, and xylose increased by 0.74%, 490.54%, 1.56%, and 87.79% respectively, and enzymolysis made fucose detectable, but the content of glucose and arabinose decreased by 11.05%, 8.66%. After enzymolysis, the dandelion polysaccharide became rough, with more pores and loose structure, but the reasons need to be further explored. The experimental research results would provide a certain theoretical basis for the in-depth development and utilization of dandelion resources.