Rapid Determination of 11 Mycotoxins in Coffee Bean through Multifunctional Purification and Immunoaffinity Column Coupled to High-performance Liquid Chromatography Tandem Mass Spectrometry

CHEN Yueming; HUANG Jingchu; XU Ting; LIN Hong; CAI Weiyi; ZHONG Yuxin; SU Yanyu; CHEN Jiaxin; HU Huixiang; WANG Yu

doi:10.13386/j.issn1002-0306.2020110294

Volume 42 Issue 16

Aug. 2021

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Science and Technology of Food Industry > 2021 > 42(16): 285-293. > DOI: 10.13386/j.issn1002-0306.2020110294

CHEN Yueming, HUANG Jingchu, XU Ting, et al. Rapid Determination of 11 Mycotoxins in Coffee Bean through Multifunctional Purification and Immunoaffinity Column Coupled to High-performance Liquid Chromatography Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(16): 285−293. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110294.

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Rapid Determination of 11 Mycotoxins in Coffee Bean through Multifunctional Purification and Immunoaffinity Column Coupled to High-performance Liquid Chromatography Tandem Mass Spectrometry

1.
Guangzhou Food Inspection Institute, Guangzhou 511400, China
2.
Guangdong Meiweixian Flavoring Foods Co., Ltd., Zhongshan 528437, China
3.
Guangdong Chubang Foods Co., Ltd., Yangjiang 529800, China

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Received Date: December 01, 2020
Available Online: June 14, 2021

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Abstract

Abstract

A method was established for the simultaneous determination of 11 fungal toxins in coffee beans by ultra-performance liquid chromatography tandem mass spectrometry, the effects of column selection, extracting solvent composition, solid phase extraction column selection and flow phase ratio on the analysis results were examined. Taking coffee beans as the research object, the samples were extracted by acetylene-water-formic acid (85+14+1, V/V) purified by Oasis PRiME HLB, the purification diluted with phosphoric acid buffer, nitrogen blowing after the purified by multi-functional immunoaffinity column, determined by ultra-performance liquid chromatography tandem mass spectrometry after resoluble with 50% acetylene, with 0.1% foric acid-2 mmol/L ammonium acetate solution-acetylene as the flow phase, separated by Waters BEH C₁₈ column, determined by ultra-performance liquid chromatography tandem mass spectrometry quantitated by internal standard method. The results showed that, the linear correlation of 11 mycotoxins were greater than 0.998, the limits of detection were in the range of 0.008~0.544 μg/kg. The average recoveries at the three spiked levels were 80.2%~114% with relative standard deviations of 1.4%~7.9%. This method was simple, fast and efficient, and would be suitable for the co-measurement and analysis of 11 mycotoxins in coffee beans.

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