Analysis of Fungal Diversity of Yak Yogurt Collected and Preserved at Different Temperatures in Gannan Pastoral Area Based on High-throughput Sequencing

ZHANG Wenqi; MA Heping; LIU Caiyun; WANG Jie; PENG Zhangpu; SHAO Jianning

doi:10.13386/j.issn1002-0306.2020110233

Volume 42 Issue 13

Jun. 2021

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Science and Technology of Food Industry > 2021 > 42(13): 163-169. > DOI: 10.13386/j.issn1002-0306.2020110233

ZHANG Wenqi, MA Heping, LIU Caiyun, et al. Analysis of Fungal Diversity of Yak Yogurt Collected and Preserved at Different Temperatures in Gannan Pastoral Area Based on High-throughput Sequencing[J]. Science and Technology of Food Industry, 2021, 42(13): 163−169. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110233.

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Analysis of Fungal Diversity of Yak Yogurt Collected and Preserved at Different Temperatures in Gannan Pastoral Area Based on High-throughput Sequencing

1.
Key Laboratory of Microbial Resources Exploitation and Application of Gansu Province, Institute of Biology, Gansu Academy of Sciences, Lanzhou 730000, China
2.
Gansu Academy of Sciences, Lanzhou 730000, China

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Received Date: November 05, 2020
Available Online: May 10, 2021

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Abstract

Abstract

Illumina NovaSeq high-throughput sequencing technology was used to sequence the fungi gene ITS2 regions of yak yogurt samples in the Gannan pastoral area, OTU clustering, alpha diversity, and beta diversity were analyzed. Through analyzing and comparing the fungal composition and diversity of yak yogurt collected at different preservation temperatures in the Gannan pastoral area, the results showed that the main fungal in yak yogurt in Gannan pastoral area was Ascomycota at the phylum level, the abundance value was 82.09%~99.25%; at the genus level, among which Saccharomyces was the dominant with the abundance value was 23.98%~63.49%, followed by Kluyveromyces with the abundance value 16.08%~31.89%, Pichia with the abundance value 3.39%~35.21%. There was no significant difference in fungal diversity among yak yogurt samples which were collected at four different preservation temperatures in the Gannan pastoral area, the fungal community composition of the sampling groups was similar at all levels of classification, with only some differences in composition ratio.
- yak yogurt,
- fungal diversity,
- different collection and preservation temperatures,
- high-throughput sequencing

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References (32)

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