Analysis of Gene Clusters for Sanxiapeptin Biosynthesis by Transcriptomic Sequencing

LIU Chao; LI Kun; BAI Xiaoxuan; YANG Yuchun; XUE Yanhong; LIU Shiping

doi:10.13386/j.issn1002-0306.2020110192

Volume 42 Issue 13

Jun. 2021

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Science and Technology of Food Industry > 2021 > 42(13): 156-162. > DOI: 10.13386/j.issn1002-0306.2020110192

LIU Chao, LI Kun, BAI Xiaoxuan, et al. Analysis of Gene Clusters for Sanxiapeptin Biosynthesis by Transcriptomic Sequencing [J]. Science and Technology of Food Industry, 2021, 42(13): 156−162. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110192.

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Analysis of Gene Clusters for Sanxiapeptin Biosynthesis by Transcriptomic Sequencing

College of Life Science and Pharmacy, Key Laboratory of Functional Yeast (China National Light Industry), Three Gorges University, Yichang 443002, China

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Received Date: November 22, 2020
Available Online: April 22, 2021

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Abstract

Abstract

In order to explore the biosynthesis mechanism of the sanxiapeptin, the BGISEQ-500 platform was used to determine cDNA library when the SG-4 was cultured at 200 mL for 4, 7 d and 300 mL for 7 d, and RNA-seq analysis 6.42 Gb data generated by high-throughput sequencing without reference genome. The results showed that the transcriptome sequence alignment rate was as high as 93.88%, it was found that the differential genes were mainly concentrated in the pathways of antibiotic synthesis, substance transport and redox metabolism. In the non-ribosomal peptide synthetase (NRPS, Non-ribosomal peptide synthetase) gene cluster PDE_01071, the expression trends of 21 genes were completely consistent with the content of substances, it suggested that the gene cluster PDE_01071 might be responsible for the synthesis of sanxiapeptin.
- penicillium oxalicum,
- sanxiapeptin,
- citrus,
- biosynthesis,
- comparative transcriptome,
- gene cluster

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