Inhibitory Effect of Genkwanin on <i>α</i>-Glucosidase

JIANG Youya; CHEN Qi; ZHANG Lu; GAO Xue

doi:10.13386/j.issn1002-0306.2020110126

Volume 42 Issue 15

Jul. 2021

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Science and Technology of Food Industry > 2021 > 42(15): 43-47. > DOI: 10.13386/j.issn1002-0306.2020110126

JIANG Youya, CHEN Qi, ZHANG Lu, et al. Inhibitory Effect of Genkwanin on α-Glucosidase[J]. Science and Technology of Food Industry, 2021, 42(15): 43−47. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110126.

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Inhibitory Effect of Genkwanin on α-Glucosidase

1.
College of Environment and Resources, Chongqing Technology and Business University, Chongqing 400067, China
2.
Chongqing Shenghuaxi Pharmaceutical Corporation, Chongqing 400061, China

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Received Date: November 12, 2020
Available Online: June 02, 2021

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Abstract

Abstract

To investigate the inhibitory activity and mechanism of genkwanin on α-glucosidase, the inhibitory activity was determined by ultraviolet and fluorescence spectroscopy, including the inhibitory activity, inhibitory type of genkwanin on α-glucosidase and the fluorescence quenching properties. The results showed that the genkwanin had a significant inhibitory effect on α-glucosidase in a competitive manner. Genkwanin interacted with α-glucosidase was mainly driven by hydrogen bond and Van der Waals force with the binding sites of 2.8 and 2.2 at 291 and 310 K, respectively. The formation of genkwanin and α-glucosidase complexes resulted in fluorescence quenching of α-glucosidase and thereby inhibited the activity of α-glucosidase.
- genkwanin,
- α-glucosidase,
- inhibitory effect,
- fluorescence quenching

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