Improving Effect of the Composite Granules of Bitter Melon and Mulberry Leaf on Lipid-Glucose Metabolism and Abnormal Intestinal Mucosal Barrier in High-Fat Diet-Induced Obese Rats

CHENG Xiaoxiao; ZHANG Miaoxin; WANG Man; GENG Xufang; HU Jingyi; WANG Zhen; GAO Jiameng; WANG Yawen; LI Li; ZHAO Ding

doi:10.13386/j.issn1002-0306.2020110096

Volume 42 Issue 15

Jul. 2021

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Science and Technology of Food Industry > 2021 > 42(15): 337-342. > DOI: 10.13386/j.issn1002-0306.2020110096

CHENG Xiaoxiao, ZHANG Miaoxin, WANG Man, et al. Improving Effect of the Composite Granules of Bitter Melon and Mulberry Leaf on Lipid-Glucose Metabolism and Abnormal Intestinal Mucosal Barrier in High-Fat Diet-Induced Obese Rats[J]. Science and Technology of Food Industry, 2021, 42(15): 337−342. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110096.

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Improving Effect of the Composite Granules of Bitter Melon and Mulberry Leaf on Lipid-Glucose Metabolism and Abnormal Intestinal Mucosal Barrier in High-Fat Diet-Induced Obese Rats

College of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China

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Received Date: November 10, 2020
Available Online: June 01, 2021

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Abstract

Objective: To study the effect of the composite granules of bitter melon and mulberry leaf (CGBM) on improving dysfunction of obesity rats induced by high-fat diet. An explorative study was conducted on CGBM for its regulation of lipid-glucose metabolism and intestinal mucosal barrier in high-fat diet-induced obese rats, in order to provide data support for the further development of CGBM. Methods: SD rats were randomly divided into control group, model group, CGBM low-dose and high-dose group. The rats in the control group were given basic diet, whereas the others were fed in the high-fat diet to establish obesity model. The rats in the low-dose (0.5 g/kg) and high-dose (1.0 g/kg) groups were given CGBM by intragastric administration per day for 8 weeks. The rats in other groups were given the same volumes of normal saline by the same method for 8 weeks. The body weight, serum glucose and lipid levels (such as GLU, GSP, TC, TG, HDL-C, LDL-C) of rats were measured. The morphology of liver and colon tissues were observed by HE staining, and the expression of tight junction protein occludin in colon tissues were detected. Results: CGBM could effectively reduce the body weight, and lower the high level of serum glucose and lipid in obese rats. The phenomenon of excessive lipid droplet in liver and the abnormal morphology of glands in colon mucosa were also improved by CGBM. The results also indicated that the CGBM could effectively ameliorate the lower expression level of occludin protein in obesity rats (P<0.05). Conclusion: CGBM has the obvious improvement effect on dysfunction of glucose and lipid metabolism in obesity rats induced by high-fat diet. The hepatic and intestinal injuries caused by high-fat diet were also repaired by CGBM. This study would have an important significance on accelerating the development of CGBM and its mechanism research.
- bitter melon,
- mulberry leaf,
- high fat diet,
- obese rats,
- glucose-lowering,
- lipid-lowering,
- intestinal mucosal barrier

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References

[1]	Thomas E L, Frost G, Taylor-Robinson S D, et al. Excess body fat in obese and normal-weight subjects[J]. Nutrition Research Reviews,2012,25(1):150−161. doi: 10.1017/S0954422412000054
[2]	Smith K B, Smith M S. Obesity statistics[J]. Primary Care,2016,43(1):121−135. doi: 10.1016/j.pop.2015.10.001
[3]	Martinez K B, Leone V, Chang E B. Western diets, gut dysbiosis, and metabolic diseases: Are they linked?[J]. Gut Microbes,2017,8(2):130−142. doi: 10.1080/19490976.2016.1270811
[4]	Grover J K, Yadav S P. Pharmacological actions and potential uses of Momordica charantia: A review[J]. Journal of Ethnopharmacology,2004,93(1):123−132. doi: 10.1016/j.jep.2004.03.035
[5]	Peter E L, Mtewa A G, Nagendrappa P B, et al. Systematic review and meta-analysis protocol for efficacy and safety of Momordica charantia L. on animal models of type 2 diabetes mellitus[J]. Systematic Reviews,2020,9(1):7. doi: 10.1186/s13643-019-1265-4
[6]	贾福怀, 许璐云, 王彩霞, 等. 降糖类保健食品配方及功效成分研究现状与展望[J]. 食品与发酵工业,2017,43(10):277−282.
[7]	He X, Fang J, Ruan Y, et al. Structures, bioactivities and future prospective of polysaccharides from Morus alba (white mulberry): A review[J]. FoodChemistry,2018,245:899−910.
[8]	Cai S Y, Sun W, Fan Y X, et al. Effect of mulberry leaf (Folium mori) on insulin resistance via IRS-1/PI3K/Glut-4 signalling pathway in type 2 diabetes mellitus rats[J]. Pharmaceutical Biology,2016,54(11):2685−2691. doi: 10.1080/13880209.2016.1178779
[9]	吴雯, 梁凯伦, 陈波, 等. 桑叶提取物对食源性肥胖大鼠的减肥作用及机制研究[J]. 中国中药杂志,2017,42(9):1757−1761.
[10]	王祖文, 秦樱瑞, 黄先智, 等. 桑叶-苦瓜混合粉对正常小鼠血糖血脂和抗氧化能力的影响[J]. 食品科学,2019,40(9):166−172.
[11]	彭晓蝶, 秦樱瑞, 黄先智, 等. 桑叶-苦瓜混合粉对糖尿病小鼠的降糖作用[J]. 现代食品科技,2017,33(4):31−37.
[12]	杨敏, 丁晓雯, 秦樱瑞, 等. 桑叶-苦瓜混合粉对糖尿病小鼠血脂的影响[J]. 现代食品科技,2017,33(8):12−17.
[13]	陈小敏, 谭书明, 黄颖, 等. 刺梨、桑叶、苦瓜饮料对糖尿病小鼠的降糖作用[J]. 中国酿造,2019,38(6):123−127. doi: 10.11882/j.issn.0254-5071.2019.06.023
[14]	唐晓荞, 刘春霞, 刘瑶, 等. 苦瓜、桑叶合剂安全性毒理学研究[J]. 公共卫生与预防医学,2013,24(5):17−19.
[15]	Upadhyay A, Agrahari P, Singh D K. A Review on salient pharmacological features of Momordica charantia[J]. International Journal of Pharmacology,2015,11(5):405−413. doi: 10.3923/ijp.2015.405.413
[16]	张晓寒, 张程慧, 于文睿, 等. 药食同源类植物多糖降血糖功效的研究进展[J]. 食品安全质量检测学报,2018,9(14):3699−3705. doi: 10.3969/j.issn.2095-0381.2018.14.025
[17]	张瑞婷, 周涛, 宋潇潇, 等. 葛根的活性成分及其药理作用研究进展[J]. 安徽农学通报,2018,24(1):15−17. doi: 10.3969/j.issn.1007-7731.2018.01.007
[18]	王萌萌, 梅振东, 张淼, 等. 葛根提取物对高脂血症大鼠血脂及抗氧化能力的影响[J]. 食品工业科技,2015,36(11):369−372.
[19]	冯欣煜, 姚志凌, 李明辉. 金银花的现代药理和临床运用研究进展[J]. 中医研究,2015,28(9):78−80. doi: 10.3969/j.issn.1001-6910.2015.09.37
[20]	朱媛媛, 卞勇, 宋志秀, 等. n-3多不饱和脂肪酸对小鼠糖脂代谢影响及机制[J]. 食品科技,2020,45(8):227−232.
[21]	麻思萌, 覃秋芳, 马敏洁, 等. 平卧菊三七醇提物对溃疡性结肠炎的改善作用[J]. 现代食品科技,2020,36(2):12−17,193.
[22]	杜娟, 朱安峰, 宋东明, 等. 高脂饮食诱导的肥胖大鼠肝脏形态学改变及意义[J]. 中华内分泌外科杂志,2019(6):463−465. doi: 10.3760/cma.j.issn.1674-6090.2019.06.005
[23]	Li Y, Jin D X, Xie W X, et al. PPAR-γ and Wnt regulate the differentiation of MSCs into adipocytes and osteoblasts respectively[J]. Current Stem Cell Research & Therapy,2018,13(3):185−192.
[24]	Popovich D G, Li L, Zhang W. Bitter melon (Momordica charantia) triterpenoid extract reduces preadipocyte viability, lipid accumulation and adiponectin expression in 3T3-L1 cells[J]. Food and Chemical Toxicology,2010,48(6):1619−1626. doi: 10.1016/j.fct.2010.03.035
[25]	Tontonoz P, Hu E, Spiegelman B M. Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor[J]. Cell,1994,79(7):1147−1156. doi: 10.1016/0092-8674(94)90006-X
[26]	Iozzo P, Bucci M, Roivainen A, et al. Fatty acid metabolism in the liver, measured by positron emission tomography, is increased in obese individuals[J]. Gastroenterology,2010,139(3):846−856. doi: 10.1053/j.gastro.2010.05.039
[27]	刘美宏, 刘回民, 谢佳函, 等. 玉米黄素调控肥胖小鼠肝脏脂质及能量代谢作用[J]. 中国食品学报,2019,19(12):31−38.
[28]	Cani P D, Bibiloni R, Knauf C, et al. Changes in Gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice[J]. Diabetes,2008,57(6):1470−1481. doi: 10.2337/db07-1403
[29]	König J, Wells J, Cani P D, et al. Human intestinal barrier function in health and disease[J]. Clinical & Translational Gastroenterology,2016,7(10):e196.
[30]	Bai J, Zhu Y, Dong Y. Obese rats supplemented with bitter melon display marked shifts in the expression of genes controlling inflammatory response and lipid metabolism by RNA-Seq analysis of colonic mucosa[J]. Genes & Genomics,2018,40(6):561−567.
[31]	Guo H L, Xu Y H, Huang W, et al. Kuwanon G preserves LPS-induced disruption of gut epithelial barrier in vitro[J]. Molecules,2016,21(11):1597. doi: 10.3390/molecules21111597
[32]	Fan M, Kim E K, Choi Y J, et al. The role of Momordica charantia in resisting obesity[J]. International Journal of Environmental Research and Public health,2019,16(18):3251. doi: 10.3390/ijerph16183251

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Science and Technology of Food Industry

Improving Effect of the Composite Granules of Bitter Melon and Mulberry Leaf on Lipid-Glucose Metabolism and Abnormal Intestinal Mucosal Barrier in High-Fat Diet-Induced Obese Rats