JIANG Tian, LU Wenwei, CUI Shumao, et al. Characteristic Analysis of Different Microencapsulated Bifidobacterium [J]. Science and Technology of Food Industry, 2021, 42(11): 128−134. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020100241.
Citation: JIANG Tian, LU Wenwei, CUI Shumao, et al. Characteristic Analysis of Different Microencapsulated Bifidobacterium [J]. Science and Technology of Food Industry, 2021, 42(11): 128−134. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020100241.

Characteristic Analysis of Different Microencapsulated Bifidobacterium

More Information
  • Received Date: October 28, 2020
  • Available Online: April 02, 2021
  • To analyse the characteristic of different microencapsulated Bifidobacterium in simulated digestive tract, different Bifidobacterium were embedded by electrostatic spray, conventional spray and emulsification freezing method. The survival rate, hydrophobicity, copolymerization, adhesion and fatty acid of lipoteichoic acid were studied after passing through the simulated digestive tract. The survival rate of electrostatic spray microencapsulation was higher than traditional spray microencapsulation and emulsification freezing microencapsulation. The survival rate of electrostatic spray microencapsulated Bifidobacterium lactis BL03 was 55.01%±4.12% after passing through the simulated digestive tract.The hydrophobicity of electrostatic spray microencapsulated Bifidobacterium was 125.14%~368.75% higher than emulsification freezing microencapsulation except Bifidobacterium longum BLL2. The copolymerization of electrostatic spray microencapsulated Bifidobacterium was 112.50%~372.72% higher than emulsification freezing microencapsulation. The adhesion of electrostatic spray microencapsulated Bifidobacterium was 109.52%~411.11% higher than emulsification freezing microencapsulation except Bifidobacterium longum BLL2. The Electrostatic spray microencapsulation and traditional spray microencapsulation were better than emulsification freezing microencapsulation. The adhesion and embedding rate was (46.18±2.82)CFU/cell and 93.31%±3.16%, respectively for electrostatic spray microencapsulated Bifidobacterium lactis BL03. The content of C12:0 and C6:0 fatty acid of electrostatic spray microencapsulation and conventional spray microencapsulation were higher than emulsification freezing microencapsulation, but C18:1n9c fatty acid was lower. Electrostatic spray drying was the best microencapsulated method for Bifidobacterium in the condition of simulating digestive tract.
  • [1]
    Landete J M, Gaya P, Rodriauez E, et al. Probiotic bacteria for healthier aging: Immunomodulation and metabolism of phytoestrogens[J]. BioMed Research International,2017,209(17):93−98.
    [2]
    Short S P, Pilat J M, Williams C S. Roles for selenium and selenoprotein P in the development, procession, and prevention of intestinal disease[J]. Free Radical Biology and Medicine,2018,127:26−35. doi: 10.1016/j.freeradbiomed.2018.05.066
    [3]
    Cailard R, Lapointe N. In vitro gastric survival of commercially available probiotic strains and oral dosage forms[J]. International Journal of Pharmaceutics,2017,519(1-2):125−127. doi: 10.1016/j.ijpharm.2017.01.019
    [4]
    孙玉章, 崔栩, 马丰英, 等. 益生菌微囊化技术研究进展[J]. 动物医学进展,2018,39(7):86−89. doi: 10.3969/j.issn.1007-5038.2018.07.019
    [5]
    李康宁, 邢会霞, 焦志军, 等. 双歧杆菌NQ150双层微胶囊制备及其特性研究[J]. 中国微生态学杂志,2016,28(11):1246−1248.
    [6]
    Chen L, Yang T, Song Y, et al. Effect of xanthan-chitosan-xanthan double layer encapsulation on survival of Bifidobacterium BB01 in simulated gastrointestinal conditions, bile salt solution and yogurt[J]. LWT-Food Science and Technology,2017,81:274−280. doi: 10.1016/j.lwt.2017.04.005
    [7]
    Verruck S, Carvalho M W D, Liz G R D, et al. Survival of Bifidobacterium BB-12 microencapsulated with full-fat goat's milk and prebiotics when exposed to simulated gastrointestinal conditions and thermal treatments[J]. Small Ruminant Research,2017,153:48−56. doi: 10.1016/j.smallrumres.2017.05.008
    [8]
    Fayed B, Abood A, El-Sayed H S, et al. A symbiotic multi-particulate microcapsule for enhancing inulin intestinal release and Bifidobacterium gastro-intestinal survivability[J]. Carbohydrate Polymers,2018,193:137−143. doi: 10.1016/j.carbpol.2018.03.068
    [9]
    Eratte D, Dowling K, Barrow C J, et al. In vitro digestion of probiotic bacteria and omega-3 oil co-microencapsulated in whey protein isolate-gum Arabic complex coacervates[J]. Food Chem,2017,227:129−136.
    [10]
    Arena M P, Capozzi V, Spano G, et al. The potential of lactic acid bacteria to colonize biotic and abiotic surfaces and the investigation of their interactions and mechanisms[J]. J Appl Microbiol,2017,101(7):2641−2657.
    [11]
    Bentley-Hewitt K L, Narbad A, Majsak-Newman G, et al. Lactobacilli survival and adhesion to colonic epithelial cell lines is dependent on long chain fatty acid exposure[J]. European Journal of Lipid Science and Technology,2017:17−62.
    [12]
    Ferrando V F V, Quiberoni A Q A, Reinheiiec J R J, et al. Functional properties of Lactobacillus plantarum strains: A study in vitro of heat stress influence[J]. Food Microbiology,2016:154−161.
    [13]
    RenD, LiC, QinY, etal. Inhibition of Staphylococcus aureus adherence to Caco-2 cells by lactobacilli and cell surface properties that influence attachment[J]. Anaerobe,2012,18(5):508−515. doi: 10.1016/j.anaerobe.2012.08.001
    [14]
    Valeriano V D, ParungaoB M M, Kang D K. In vitroevaluation of the mucin- adhesion ability and probiotic potential of Lactobacillus mucosae LM1[J]. J Appl Microbiol,2014,117(2):485−497.
    [15]
    李文桂, 陈雅棠. 双歧杆菌生物学作用研究进展[J]. 地方病通报,2006,21(1):85−88. doi: 10.3969/j.issn.1000-3711.2006.01.031
    [16]
    Barbara G, Stanghellini V, Brandi G, et al. Interactions between commensal bacteria and gut sensorimotor function in health and disease[J]. Am J Gastroenterol,2005,100(11):2560−2568. doi: 10.1111/j.1572-0241.2005.00230.x
    [17]
    Kashyap P C, Marcobal A, Ursell L K, et al. Complex interactions among diet, gastrointestinal transit, and gut microbiota in humanized mice[J]. Gastroenterology,2013,144(5):967−977. doi: 10.1053/j.gastro.2013.01.047
    [18]
    Caenepeel P, Janssens J, Vantrappen G, et al. Interdigestive myoelectric complex in germ-free rats[J]. Dig Dis Sci,1989,34(8):1180−1184.
    [19]
    Castellano P, Peter I M, Longo B L, et al. Lactobacillus spp. impairthe ability of Listeria monocytogenes FBUNT to adhere to and invade Caco-2 cells[J]. Biotechnol Lett,2018,40 (8):1237−1244.
    [20]
    Rong J, Zheng H, Liu M, et al. Probiotic and anti-inflammatory attributes of an isolate Lactobacillus helveticcs NS8 from Mongolian fermented koumiss[J]. BMC Microbiol,2015,15:196−208. doi: 10.1186/s12866-015-0525-2
    [21]
    Colado M C, Meifluoto J, Salminen S. Adhesion andaggregation properties of probiotic and pathogen strains[J]. European Food Research and Technology,2008,226(5):1065−1073.
    [22]
    Gopal P K, Prasad J, Smart J, et al. Invitroadherence properties of Lactobacillusrhamnosus DR20 and Bifidobacteriumlactis dr10 strains and the antagonistic activity again an enterotoxigenic Escherichia coli[J]. International Journal of Food Microbiology,2001,67(3):207−216. doi: 10.1016/S0168-1605(01)00440-8
    [23]
    Del R B, Sgorbati B, Miglioll M, et al. Adhesion, auto aggregation and hydrophobicity of 13 strains of Bifidobacterium longum[J]. Lett Appl Microbiol,2000,31(6):438−442.
    [24]
    李清, 刘小莉, 王英, 等. 植物乳杆菌表面性质及对Caco-2 细胞的黏附[J]. 食品科学,2015,36(9):97−101. doi: 10.7506/spkx1002-6630-201509018
    [25]
    Wang Y, Deletre J, Guilot A, et a1. Influence of coling temperature and duration on cold adaptation of Lactobacillus acidophilus RD758[J]. Cryobiology,2005,50(3):294−307.
  • Cited by

    Periodical cited type(2)

    1. 赵佳佳,顾尧,李言,钱海峰,王立. 高活性天然酵母发酵剂的优化. 食品与机械. 2024(02): 197-203+212 .
    2. 滕堃如,李斌,曹梦思,陈红贺,陈楠楠,刘明,郭新光. 益生菌消化道耐受性的体外评价方法研究进展. 食品科技. 2023(11): 41-49 .

    Other cited types(4)

Catalog

    Article Metrics

    Article views (291) PDF downloads (39) Cited by(6)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return