Ginseng Oligopeptides against Benzopyrene-Induced Inflammatory Injury of Lung Epithelial Cells

GAO Chao; LIU Jie; ZHAO Chuanxin

doi:10.13386/j.issn1002-0306.2020100138

Volume 42 Issue 16

Aug. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(16): 336-343. > DOI: 10.13386/j.issn1002-0306.2020100138

GAO Chao, LIU Jie, ZHAO Chuanxin. Ginseng Oligopeptides against Benzopyrene-Induced Inflammatory Injury of Lung Epithelial Cells[J]. Science and Technology of Food Industry, 2021, 42(16): 336−343. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020100138.

Citation:

PDF (1216 KB)

Ginseng Oligopeptides against Benzopyrene-Induced Inflammatory Injury of Lung Epithelial Cells

1.
Department of Pharmacy, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510000, China
2.
State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen 518060, China
3.
Department of Science and Education, Dongying People’s Hospital, Dongying 257000, China

More Information

Received Date: October 19, 2020
Available Online: June 20, 2021

Graphical Abstract

Abstract

Abstract

In this study, ginseng oligopeptides were extracted from ginseng, and the amino acid sequence was analyzed. CCK-8 technology was used to screen the oligopeptides with the best effect on protecting human lung epithelial type II (A549) cells from benzopyrene (Bap) damage. Flow cytometry detection of ginseng oligopeptide-1 (Renshen Oligopeptides-1, RSO-1) at 10 and 50 μmol/L concentration of 10 nmol/L Bap-induced A549 cell apoptosis and mitochondrial membrane. The effect of potential decreased and reactive oxygen species (ROS) content increased. Western blotting verified that Bap at a concentration of 10 nmol/L affects the Aromatic Hydrocarbon Receptor (AHR), NF-κB and MAPK signaling pathways in A549 cells and the influence of AP-1 factor, and tested the inhibitory effect of RSO-1 on Bap toxicity at 10 and 50 μmol/L. The results showed that 10 nmol/L Bap exposure induced oxidative stress in A549 cells (significantly different from the blank control group, P<0.05), activated aromatic hydrocarbon receptor (AHR) and NF-κB signaling pathway and induce cell apoptosis (compared with the blank control group, there was a significant difference, P<0.05). The concentration of 10 and 50 μmol/L ginseng oligopeptide (Ginseng Oligopeptide, RSO, RSO-1~amino acid sequence-EGHGF) could inhibit the expression of AP-1 factor and aryl hydrocarbon receptor (AHR) caused by Bap exposure and NF-κB activation, thereby inhibiting cell apoptosis was caused by Bap exposure (compared with Bap group, both have significant difference, P<0.05). This research would provide a theoretical basis for the application and in-depth development of ginseng.
- ginseng,
- benzopyrene,
- oligopeptide,
- aromatic hydrocarbon receptor,
- NF-κB

FullText(HTML)

References (29)

References

[1]	黎阳, 张铁军, 刘素香, 等. 人参化学成分和药理研究进展[J]. 中草药,2009,40(1):164−166.
[2]	姚梦杰, 吕金朋, 张乔, 等. 人参化学成分及药理作用研究[J]. 吉林中医药,2017,37(12):1261−1263.
[3]	Zhang W, Ye L, Wang F, et al. Immunomodulatory effects of the Meretrix oligopeptide (QLNWD) on immune-deficient mice[J]. Molecules,2019,24(24):4452. doi: 10.3390/molecules24244452
[4]	Shao J J, Yao Z, Wu H, et al. Study on extraction of antioxidant oligopeptide from oyster shell[J]. Journal of Chinese Medicinal Materials,2013,36(9):1395−1397.
[5]	Denning N L, Yang W L, Hansen L, et al. C23, an oligopeptide derived from cold-inducible RNA-binding protein, suppresses inflammation and reduces lung injury in neonatal sepsis[J]. J Pediatr Surg,2019,54(10):2053−2060. doi: 10.1016/j.jpedsurg.2018.12.020
[6]	Makra L, Tombácz S, Bálint B, et al. Influences of meteorological parameters and biological and chemical air pollutants on the incidence of asthma and rhinitis[J]. Climate Research,2015,37(1):99−119.
[7]	刘强, 高建华, 石勇, 等. 北黄海北部表层沉积物中多环芳烃的分布特征及控制因素分析[J]. 海洋环境科学,2020(1):53−58. doi: 10.12111/j.mes20200108
[8]	Gao M, Li Y, Sun Y, et al. A common carcinogen benzo[a]pyrene causes p53 overexpression in mouse cervix via DNA damage[J]. Mutation Research/fundamental & Molecular Mechanisms of Mutagenesis,2011,724(1−2):69−75.
[9]	Toydemir G, Loonen L M P, Venkatasubramanian P B, et al. Coffee induces AHR- and Nrf2-mediated transcription in intestinal epithelial cells[J]. Food Chem,2020,341(Pt 2):128261.
[10]	Hitzman R T, Dunlap T L, Howell C E, et al. 6-Prenylnaringenin from hops disrupts eralpha-mediated downregulation of CYP1A1 to facilitate estrogen detoxification[J]. Chem Res Toxicol,2020.
[11]	Abnet C C, Fagundes R B, Strickland P T, et al. The influence of genetic polymorphisms in Ahr, CYP1A1, CYP1A2, CYP1B1, GST M1, GST T1 and UGT1A1 on urine 1-hydroxypyrene glucuronide concentrations in healthy subjects from Rio Grande do Sul, Brazil[J]. Carcinogenesis,2007,28(1):112−117. doi: 10.1093/carcin/bgl131
[12]	Ko H M, Choi S H, Kim Y, et al. Effect of Rosa laevigata on PM10-induced inflammatory response of human lung epithelial cells[J]. Evid Based Complement Alternat Med,2020,2020(4):2893609.
[13]	Lin L J, Huang H Y. DFSG, a novel herbal cocktail with anti-asthma activity, suppressed MUC5AC in A549 cells and alleviated allergic airway hypersensitivity and inflammatory cell infiltration in a chronic asthma mouse model[J]. Biomed Pharmacother,2020,121:109584. doi: 10.1016/j.biopha.2019.109584
[14]	Lawrence T. The nuclear factor NF-kappaB pathway in inflammation[J]. Cold Spring Harb Perspect Biol,2009,1(6):a001651.
[15]	杨全伟, 肖柳, 黄雄超, 等. 金蝉花多糖的含量组成分析及其对LPS诱导的THP-1细胞NF-κB活性的研究[J]. 中国现代中药,2016,18(9):1129−1134.
[16]	张莉萍, 蒋纪恺, Tam J. Western blot检测蛋白表达的方法改进及初步应用[J]. 第三军医大学学报,2001(10):112−113.
[17]	Pinkse M W H, Uitto P M, Hilhorst M J, et al. Selective isolation at the femtomole level of phosphopeptides from proteolytic digests using 2D-NanoLC-ESI-MS/MS and titanium oxide precolumns[J]. Analytical Chemistry,2004,76(14):3935−3943. doi: 10.1021/ac0498617
[18]	Han M, Ye S, Wen W, et al. Oxidative stress-mediated pro-inflammatory responses in lung epithelial cells exposed to multi-walled carbon nanotubes[C]. //The 4th International Conference on Bioinformatics and Biomedical Engineering, 2010: 1−4.
[19]	Stern M, Meagher L C, Savill J, et al. Apoptosis in human eosinophils: Programmed cell death in the eosinophil leads to phagocytosis by macrophages and is modulated by IL-5[J]. Journal of Immunology,1992,148(11):3543−3549.
[20]	Luo Z, Xu X, Sho T, et al. ROS-induced autophagy regulates porcine trophectoderm cell apoptosis, proliferation, and differentiation[J]. Am J Physiol Cell Physiol,2019,316(2):C198−C209. doi: 10.1152/ajpcell.00256.2018
[21]	Fehrenbach E, Northoff H. Free radicals, exercise, apoptosis, and heat shock proteins[J]. Exercise Immunology Review,2001,7(1):66−89.
[22]	Qian W, Kumar N, Roginskaya V, et al. Chemoptogenetic damage to mitochondria causes rapid telomere dysfunction[J]. Proc Natl Acad Sci USA,2019,116(37):18435−18444. doi: 10.1073/pnas.1910574116
[23]	袁建成, 程君涛, 秦孝健, 等. 线粒体细胞色素C释放在H₂O₂诱导内皮细胞凋亡中的作用[J]. 中华烧伤杂志,2001,17(6):360−363. doi: 10.3760/cma.j.issn.1009-2587.2001.06.013
[24]	Oberacker T, Bajorat J, Ziola S, et al. Enhanced expression of thioredoxin-interacting-protein regulates oxidative DNA damage and aging[J]. FEBS Lett,2018,592(13):2297−2307. doi: 10.1002/1873-3468.13156
[25]	Mitchell J P, Carmody R J. NF-kappaB and the transcriptional control of inflammation[J]. Int Rev Cell Mol Biol,2018,335:41−84.
[26]	Lu Y, Suh S-J, Kwak C-H, et al. Saucerneol F, a new lignan, inhibits iNOS expression via MAPKs, NF-κB and AP-1 inactivation in LPS-induced RAW264.7 cells[J]. International Immunopharmacology,2012,12(1):175−181. doi: 10.1016/j.intimp.2011.11.008
[27]	Gunhild Trøen, Vigdis Nygaard, Tor-Kristian Jenssen, et al. Constitutive expression of the AP-1 transcription factors c-jun, junD, junB, and c-fos and the marginal zone B-Cell transcription factor notch2 in splenic marginal zone lymphoma[J]. Journal of Molecular Diagnostics,2004,6(4):297−307. doi: 10.1016/S1525-1578(10)60525-9
[28]	Wang E, Liu X, Tu W, et al. Benzo(a)pyrene facilitates Dermatophagoides group 1(Der f 1)-induced epithelial cytokine release through aryl hydrocarbon receptor in asthma[J]. Allergy, 2019, 74(9): 1675−1690.
[29]	Su C M, Hou G G, Wang C H, et al. Potential multifunctional agents with anti-hepatoma and anti-inflammation properties by inhibiting NF-кB activation[J]. J Enzyme Inhib Med Chem, 2019, 34(1): 1287−1297.

Supplements (0)

Cited By

Cited by

Periodical cited type(9)

1.	宋德方，李洪淼，许嘉媛，赵佳怡，刘金花. 罗汉果苷零蔗糖酸奶的研制及品质分析. 辽宁农业职业技术学院学报. 2025(02): 11-14 .
2.	李春冬，徐同，高缘，刘国强，呼日，徐伟良，马信雅，高志海，吉日嘎拉图，郭梁. 高脂酸乳与普通酸乳品质对比分析. 乳业科学与技术. 2025(02): 9-14 .
3.	段泊安，李倩文，王晓楠，陈树兴. 山茶花低糖酸奶工艺优化及其抗氧化活性分析. 食品安全质量检测学报. 2024(09): 271-277 .
4.	周洋，黄亚杰，文进. 响应面法优化无乳糖酸奶的配方研究. 中国酿造. 2024(06): 189-194 .
5.	於荣荣，孙欣燕，周頔，徐升，韩彬，汤泉，董艺凝. 基于混料设计研究代糖配比及其对炼乳品质的影响. 食品安全质量检测学报. 2024(15): 147-157 .
6.	徐广新，杨仁琴，周炜，张海霞，华惠，印伯星，王来娣. 响应面法优化桂花酒酿酸奶制备工艺. 食品安全质量检测学报. 2024(23): 145-151 .
7.	尹丽萍，张剑林，殷娜，黎进雪，王妍凌，达菊庆，李宁，武运. 模糊数学综合评价法结合响应面法优化红葡萄酒风味发酵乳工艺. 中国酿造. 2023(01): 168-173 .
8.	沈雍徽，陈娜，邢宇，黄威. 不同糖醇对凝固型酸奶品质的影响. 中国乳业. 2023(12): 86-91 .
9.	谭丽丽，程雅芳，付晶晶. 罗汉果食品开发研究进展. 食品安全导刊. 2022(10): 184-186+192 .