Protective Effects of Propolis Ethanol Extract on Mouse Aortic Endothelial Cells Injury

YANG Bo; ZHU Yuxuan; MIAO Xiaoqing; XU Xiaolan; YANG Wenchao

doi:10.13386/j.issn1002-0306.2020100065

Volume 42 Issue 15

Jul. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(15): 332-336. > DOI: 10.13386/j.issn1002-0306.2020100065

YANG Bo, ZHU Yuxuan, MIAO Xiaoqing, et al. Protective Effects of Propolis Ethanol Extract on Mouse Aortic Endothelial Cells Injury[J]. Science and Technology of Food Industry, 2021, 42(15): 332−336. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020100065.

Citation:

PDF (905 KB)

Protective Effects of Propolis Ethanol Extract on Mouse Aortic Endothelial Cells Injury

YANG Bo^1,,
ZHU Yuxuan¹,
MIAO Xiaoqing³,
XU Xiaolan^{2, 3, ,},
YANG Wenchao^{1, 2, 3, ,}

1.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350000, China
2.
College of Animal Science (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350000, China
3.
Bee Product Processing and Application Research Center of the Ministry of Education, Fuzhou 350000, China

More Information

Received Date: October 11, 2020
Available Online: May 30, 2021

Graphical Abstract

Abstract

Abstract

Objective: The aim of this study was to investigate the protective effects of ethanol extracts of propolis (EEP) on mouse aortic endothelial cells (MAEC) from injury induced by lipopolysaccharide (LPS). Methods: Cell proliferation rate was determined by CCK-8. Then MAEC were divided into the blank group, LPS model group, low-dose (2.5 μg/mL), middle-dose (5 μg/mL), and high-dose (10 μg/mL) groups of EEP. The TNF-α and IL-6 levels were evaluated by ELISA. The expression levels of ICAM-1, VCAM-1 and MCP-1 were measured by Western Blot. Results: Compared with the control group, the cell proliferation rate of MAEC in the LPS group was extremely significant reduced (P<0.001), and the levels of ICAM-1, VCAM-1, MCP-1, TNF-α and IL-6 were extremely significant increased (P<0.001). The cell proliferation rate of EEP groups increased significantly (P<0.05 or P<0.01), and the content of TNF-α, IL-6 and the expression levels of ICAM-1, VCAM-1, and MCP-1 decreased compared with these of LPS group. There were significant differences between each propolis group and LPS group (P<0.01 or P<0.001). Conclusion: EEP could effectively inhibit LPS-induced the expression of inflammatory factors in MAEC, and it has the effect of protecting endothelial cells.
- propolis,
- mouse aortic endothelial cells,
- lipopolysaccharide,
- anti-inflammatory,
- inflammatory factor

FullText(HTML)

References (36)

References

[1]	胡钟竞, 王杰. 动脉粥样硬化形成机制及影响因素研究概况[J]. 临床医药文献电子杂志,2020,50(7):197−198.
[2]	Taleb Soraya. Inflammation in atherosclerosis[J]. Archives of Cardiovascular Diseases,2016,109(12):708−715. doi: 10.1016/j.acvd.2016.04.002
[3]	Small D M. Cellular mechanisms for lipid deposition in atherosclerosis (first of two parts)[J]. The New England Journal of Medicine,1977,297(16):873−877. doi: 10.1056/NEJM197710202971608
[4]	刘俊田. 动脉粥样硬化发病的炎症机制的研究进展[J]. 西安交通大学学报(医学版),2015,36(2):141−152.
[5]	全国蜂产品标准化工作组(SAC/SWG 2). GB/T 24283-2018蜂胶[S]. 国家市场监督管理总局, 中国国家标准化管理委员会, 2018.
[6]	Huang Y L, Huang Z Q, Watanabe C, et al. Combined direct analysis in real-time mass spectrometry (DART-MS) with analytical pyrolysis for characterization of Chinese crude propolis[J]. Journal of analytical and applied pyrolysis,2019,137:227−236. doi: 10.1016/j.jaap.2018.11.030
[7]	张婷婷. GC-MS和紫外光谱法分析中国蜂胶乙醇提取物[D]. 南昌: 南昌大学, 2017.
[8]	Santos L M, Fonseca M S, Sokolonski Ana R, et al. Propolis: Types, composition, biological activities, and veterinary product patent prospecting[J]. Journal of the Science of Food and Agriculture,2020,100(4):1369−1382. doi: 10.1002/jsfa.10024
[9]	Xu X L, Pu R X, Li Y J, et al. Chemical compositions of propolis from china and the united states and their antimicrobial activities against Penicillium notatum[J]. Molecules,2019,24(19):3576. doi: 10.3390/molecules24193576
[10]	Okińczyc P, Paluch E, Franiczek R, et al. Antimicrobial activity of Apis mellifera L. and Trigona sp. propolis from Nepal and its phytochemical analysis[J]. Biomedicine & Pharmacotherapy,2020,129:110435.
[11]	Zhang H, Fu Y Y, Niu F G, et al. Enhanced antioxidant activity and in vitro release of propolis by acid-induced aggregation using heat-denatured zein and carboxymethyl chitosan[J]. Food Hydrocolloids,2018,81:104−112. doi: 10.1016/j.foodhyd.2018.02.019
[12]	Annie R P, Mikhael H F, Rodrigues T, et al. Green propolis increases myeloid suppressor cells and CD4 + Foxp3 + cells and reduces Th2 inflammation in the lungs after allergen exposure[J]. Journal of Ethno Pharmacology,2020,252:112496.
[13]	Shi Y Z, Liu Y C, Zheng Y F, et al. Ethanol extract of chinese propolis attenuates early diabetic retinopathy by protecting the blood retinal barrier in streptozotocin induced diabetic rats[J]. Journal of Food Science,2019,84(2):358−369. doi: 10.1111/1750-3841.14435
[14]	Lima L D C, Andrade S P, Campos P P, et al. Brazilian green propolis modulates inflammation, angiogenesis and fibrogenesis in intraperitoneal implant in mice[J]. BMC Complement Altern Med,2014,14:177. doi: 10.1186/1472-6882-14-177
[15]	Franchin M, Freires I A, Lazarini J G, et al. The use of Brazilian propolis for discovery and development of novel anti-inflammatory drugs[J]. European Journal of Medicinal Chemistry,2018,153:49−55. doi: 10.1016/j.ejmech.2017.06.050
[16]	Kitamura H, Saito K, Fujimoto J, et al. Brazilian propolis ethanol extract and its component kaempferol induce myeloid-derived suppressor cells from macrophages of mice in vivo and in vitro[J]. BMC complementary medicine and therapies,2018,18(1):138. doi: 10.1186/s12906-018-2198-5
[17]	Fang Y, Sang H, Yuan N, et al. Ethanolic extract of propolis inhibits atherosclerosis in Apo E-knockout mice[J]. Lipids in Health and Disease,2013,12:123. doi: 10.1186/1476-511X-12-123
[18]	赵胜男. 白杨素通过抑制NF-κB信号通路缓解血管内皮炎症[D]. 武汉: 华中科技大学, 2019.
[19]	Dauphinee S M, Karsan A. Lipopolysaccharide signaling in endothelial cells[J]. Laboratory Investigation,2006,86(1):9−22. doi: 10.1038/labinvest.3700366
[20]	任德成. 内皮细胞损伤的机制及保护药物的筛选研究[D]. 北京: 中国协和医科大学, 2002.
[21]	Zhong Y, Liu C, Feng J, et al. Curcumin affects ox-LDL-induced IL-6, TNF-α, MCP-1 secretion and cholesterol efflux in THP-1 cells by suppressing the TLR4/NF-κB/miR33a signaling pathway[J]. Experimental and Therapeutic Medicine,2020,20(3):1856−1870.
[22]	周信, 张小荣, 张秋燕, 等. 生半夏及其炮制品对小鼠主动脉内皮细胞炎性因子分泌的影响[J]. 中国实验方剂学杂志,2013,19(10):261−265.
[23]	Klinghammer L, Urschel K, Cicha K, et al. Impact of telmisartan on the inflammatory state in patients with coronary atherosclerosis Influence on IP-10, TNF-α and MCP-1[J]. Cytokine,2013,62(2):290−296. doi: 10.1016/j.cyto.2013.02.001
[24]	Zhang Y, Yang X, Bian F, et al. TNF-α promotes early atherosclerosis by increasing transcytosis of LDL across endothelial cells: crosstalk between NF-κ B and PPAR-γ[J]. Journal of Molecular and Cellular Cardiology,2014,72:85−94. doi: 10.1016/j.yjmcc.2014.02.012
[25]	张伟洁, 郑宏. IL-6介导免疫炎性反应作用及其与疾病关系的研究进展[J]. 细胞与分子免疫学杂志,2017,33(5):699−703.
[26]	解慧梅, 胡格, 索占伟, 等. 人参皂甙Rb1和黄芪多糖对微血管内皮细胞分泌NO、IL-6和TNF-α的影响[J]. 畜牧兽医学报,2006(9):903−907. doi: 10.3321/j.issn:0366-6964.2006.09.013
[27]	郭建恩, 高飞, 胡亚涛, 等. 瓜蒌薤白半夏汤对动脉粥样硬化小鼠炎症因子、ICAM-1、VCAM-1表达的影响[J]. 暨南大学学报(自然科学与医学版),2017,38(3):234−239.
[28]	黄志广. 重组SAK-HV对小鼠主动脉内皮细胞的作用及机制研究[D]. 南宁: 广西医科大学, 2016.
[29]	Kan I, Takeshi K, Hiroyuki I, et al. Induction of ICAM-1 and VCAM-1 on the mouse lingual lymphatic endothelium with TNF-α[J]. Acta Histochemica et Cytochemica,2008,41(5):115−120. doi: 10.1267/ahc.08017
[30]	Liang C J, Lee C W, Sung H C, et al. Magnolol reduced TNF-α-induced vascular cell adhesion molecule-1 expression in endothelial cells via JNK/p38 and NF-κB signaling pathways[J]. The American Journal of Chinese Medicine: An International Journal of Comparative Medicine East and West,2014,42(3):619−37.
[31]	Nageh M F, Sandberg E T, Marotti K R, et al. Deficiency of inflammatory cell adhesion molecules protects against atherosclerosis in mice[J]. Arteriosclerosis, Thrombosis, and Vascular Biology,1997,17(8):1517−1520. doi: 10.1161/01.ATV.17.8.1517
[32]	穆伟. 动脉粥样硬化中血管细胞粘附分子-1的表达与基因干预研究[D]. 济南: 山东大学, 2015.
[33]	Lin J T, Kakkar V, Lu X J. Impact of MCP-1 in atherosclerosis[J]. Current Pharmaceutical Design,2014,20(28):4580−4588. doi: 10.2174/1381612820666140522115801
[34]	Appakkudal R A, Bradley R, Ganju R K. LPS-induced MCP-1 expression in human microvascular endothelial cells is mediated by the tyrosine kinase, Pyk2 via the p38 MAPK/NF-κ B-dependent pathway[J]. Molecular immunology,2009,46(5):962−968. doi: 10.1016/j.molimm.2008.09.022
[35]	Zhang Y J, Catherine A E, Barrett J R. MCP-1: Structure/Activity Analysis[J]. Methods,1996,10(1):93−103. doi: 10.1006/meth.1996.0083
[36]	Chang M L, Guo F, Zhou Z D, et al. HBP induces the expression of monocyte chemoattractant protein-1 via the FAK/PI3K/AKT and p38 MAPK/NF-κB pathways in vascular endothelial cells[J]. Cellular Signaling,2018,43:85−94. doi: 10.1016/j.cellsig.2017.12.008

Supplements (0)

Cited By

Cited by

Periodical cited type(7)

1.	王如月，虎海防，罗莎莎，甄紫怡，徐业勇，胡晓静. 杏李不同采收成熟度果实品质分析. 中国农业科技导报(中英文). 2025(02): 158-169 .
2.	孙建城，王登亮，马创举，刘丽丽，陈骏，刘春荣，吴群. 不同采收期对华柑4号柑橘果实品质的影响. 中国果树. 2024(07): 67-73 .
3.	宋欣悦，吕靖芳，戴芬，朱作艺. 不同采收成熟度菲油果营养品质评价. 农产品质量与安全. 2024(05): 20-24+72 .
4.	方玉凤，温宝阳，王英东. 黑龙江省野生山刺玫优良种源选择利用研究. 林业科技. 2024(06): 1-6 .
5.	赵妍，祝文雪，李先宽，高鑫，付鲲，张坚. 酸枣种质资源表型多样性及营养成分分析. 种子. 2024(11): 120-126 .
6.	吴斌，苏金生，邢文婷，宋顺，马伏宁，黄东梅. 不同品种百香果果实转色期糖酸品质性状评价. 果树学报. 2024(12): 2532-2542 .
7.	李星星，周国富，骆官雨，陈思蓉，张金龙，陈国华，张晓明. 橘小实蝇对不同品种苹果的选择偏好及适应性. 中国农业科学. 2023(17): 3358-3371 .