Molecular Characteristics of Polysaccharides from <i>Radix Paeoniae Alba</i> and Its Effects on Macrophages

CAO Rongan; ZHANG Chengtai; JI Ruixue; ZHANG Jiamiao; JIA Jian; NIU Guangcai; WANG Changyuan; LI Xingge

doi:10.13386/j.issn1002-0306.2020090317

Volume 42 Issue 15

Jul. 2021

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Science and Technology of Food Industry > 2021 > 42(15): 157-162. > DOI: 10.13386/j.issn1002-0306.2020090317

CAO Rongan, ZHANG Chengtai, JI Ruixue, et al. Molecular Characteristics of Polysaccharides from Radix Paeoniae Alba and Its Effects on Macrophages [J]. Science and Technology of Food Industry, 2021, 42(15): 157−162. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020090317.

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Molecular Characteristics of Polysaccharides from Radix Paeoniae Alba and Its Effects on Macrophages

1.
College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China
2.
Agri-Food Processing and Engineering Technology Research Center of Heilongjiang Province, Daqing 163319, China

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Received Date: September 29, 2020
Available Online: May 27, 2021

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Abstract

Abstract

The polysaccharide was extracted from Radix Paeoniae Alba by the method of water extraction and ethanol precipitation. The optimum extraction parameters of the polysaccharide were determined by orthogonal test and its chemical compositions was investigated. The molecular weight of the polysaccharide was analyzed by HPSEC-UV-MALLS-RI system, which monosaccharide composition and glycosidic linkage was investigated by GC-MS. The effect of the polysaccharides on RAW264.7 cells proliferation was determined by WST assay. The results showed that the optimum extraction parameters were as follows: the ratio of liquid to material was 25:1 mL/g, extraction temperature 85 ℃, extraction time 3.0 h, with the yield of 12.40%. The polysaccharide was composed of 96.2%±1.2% total sugar and 5.5%±0.6% protein, no sulfate and uronic acid, with the molecular weight and radius of gyration 2.3×10⁶ u and 234.9 nm, respectively. The polysaccharide mainly contained glucose with the linkage of →4)-Glu-(1→, which also could promote the proliferation of RAW264.7 cells at the concentrations of 2, 5, 10 μg/mL.It was nothing that the cell proliferation rate of the 10 μg/mL treatment group was significantly increased, which exhibitedsignificant difference with other groups (P< 0.05).
- Radix Paeoniae Alba,
- polysaccharide,
- extraction procedure,
- molecular characterization,
- cell proliferation

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