Fermentation Technology Optimization of Rose Flower Enzyme Using Box-Behnken Design and  Its Antioxidant Activity Determination

XIA Guodeng; YAN Cheng; LI Linke; LI Ping; DUAN Minyan; WU Yingchuan

doi:10.13386/j.issn1002-0306.2020090304

Volume 42 Issue 13

Jun. 2021

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Science and Technology of Food Industry > 2021 > 42(13): 193-201. > DOI: 10.13386/j.issn1002-0306.2020090304

XIA Guodeng, YAN Cheng, LI Linke, et al. Fermentation Technology Optimization of Rose Flower Enzyme Using Box-Behnken Design and Its Antioxidant Activity Determination[J]. Science and Technology of Food Industry, 2021, 42(13): 193−201. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020090304.

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Fermentation Technology Optimization of Rose Flower Enzyme Using Box-Behnken Design and Its Antioxidant Activity Determination

1.
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
2.
Lijiang Chengmei Biotechnology Co., Ltd., Lijiang 674100, China
3.
Lijiang Chenghai Qinxiang Rose Manor Co., Ltd., Lijiang 674100, China

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Received Date: September 28, 2020
Available Online: May 10, 2021

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Abstract

Abstract

In this study, dried rose petals were used as the main raw material, and compound lactic acid bacteria were used for fermentation to optimize the fermentation technology of rose flower enzymes. Taking fermentation time, initial pH, inoculum amount, and fermentation temperature as investigation factors, SOD (superoxide dismutase) enzyme activity and total phenol content as evaluation indicators, response surface optimization combined with Box-Behnken design experiment to obtain the best fermentation process conditions. In addition, the research also used DPPH (1, 1-diphenyl-2-trinitrophenylhydrazine), ABTS (2, 2'-diazo-bis-3-ethylbenzothiazolin-6-sulfonic acid) and FRAP (ferric ion reduction method) method to detect the in vitro antioxidant activity of rose flower enzymes before and after fermentation, and to determine the SOD enzyme activity, total phenol content, flavonoids content, anthocyanin content, pH and titratable acid. The results showed that the optimal process conditions for the fermentation of rose flower enzyme liquid were: Fermentation time 68 h, initial pH5.4, inoculation amount 1 g, and fermentation temperature 32 ℃. Under these conditions, the SOD enzyme activity of the rose flower enzyme solution was 132.07 U/mL, the total phenol content was 6.28 mg/mL, the total flavonoid content was 3.48 mg/mL, the anthocyanin content was 5.64 mg/100 mL, and the titratable acid was 2.82 g/100 g, which was a greater improvement than before fermentation. When the rose enzyme liquid before and after fermentation and 8 mg/mL Vc volume fraction were 0.45%, the DPPH free radical scavenging rates were 73.32%, 83.70%, 35.05%, and the ABTS free radical scavenging rates were 82.18%, 92.74%, 52.82%, respectively. The FRAP values were 0.25, 0.28, and 0.115, respectively. The results of three different antioxidant methods, DPPH, ABTS, and FRAP, all showedthat rose enzyme liquid hadthe strongest antioxidant capacity after fermentation.
- fermented rose,
- fermentation,
- SOD enzyme activity,
- total phenols,
- total flavonoids,
- anthocyanin,
- antioxidant activity

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[1]	Sabokbar, Nayereh, Khodaiyan, et al. Optimization of processing conditions to improve antioxidant activities of apple juice and whey based novel beverage fermented by kefir grains[J]. Journal of Food Science and Technology,2015,52(6):3422−3432.
[2]	Imao K, Wang H, Komatsu M, et al. Free radical scavenging activity of fermented papaya preparation and its effect on lipid peroxide level and superoxide dismutase activity in iron-induced epileptic foci of rats[J]. Iubmb Life,2010,45(1):11−23.
[3]	최지수, 이설희, 박영서. Anti-diabetic effect of mulberry leaf extract fermented with Lactobacillus plantarum[J]. 2020, 52(2): 191-199.
[4]	Aruoma O I, Colognato R, Fontana I, et al. Molecular effects of fermented papaya preparation on oxidative damage, MAP Kinase activation and modulation of the benzo[a]pyrene mediated genotoxicity[J]. BioFactors,2010:26.
[5]	王子丹. 微生物酵素的研究进展[J]. 农业科技与装备,2015(8):. 67−68.
[6]	宗宁宇, 张志国. 食用玫瑰花的加工利用研究进展[J]. 农产品加工(下半月),2017(9):44−46, 52.
[7]	陈烨, 段旻燕, 严成, 等. 玫瑰花粉对面团流变学特性及酥性饼干品质的影响[J]. 食品工业科技,2020,41(19):67−71, 77.
[8]	张文, 王超, 张晶, 等. 食用玫瑰的研究进展[J]. 中国野生植物资源,2016,35(3):24−30. doi: 10.3969/j.issn.1006-9690.2016.03.008
[9]	郑淑彦, 王伟, 董金金, 等. 食用玫瑰营养保健功能及产品开发研究进展[J]. 食品研究与开发,2016,37(23):206−211. doi: 10.3969/j.issn.1005-6521.2016.23.048
[10]	Manouchehri R, Saharkhiz M J, Karami A, et al. Extraction of essential oils from damask rose using green and conventional techniques: Microwave and ohmic assisted hydrodistillation versus hydrodistillation[J]. Sustainable Chemistry & Pharmacy,2018,8:76−81.
[11]	Rakesh Kumar, Vijai K. Agnihotri, Swati Sood, et al. Agronomic interventions for the improvement of essential oil content and composition of damask rose (Rosa damascena Mill.) under western Himalayas[J]. Industrial Crops and Products,2013,48:171−177. doi: 10.1016/j.indcrop.2013.04.020
[12]	Mihalev K, Yoncheva N, Karagyozov V, et al. Colour stability improvement of strawberry beverage by fortification with polyphenoliccopigments naturally occurring in rose petals[J]. Innovative Food Science & Emerging Technologies,2007,8(3):318−321.
[13]	樊丹敏. 玫瑰花酵素加工工艺研究[J]. 现代食品,2018(9):154−157, 161.
[14]	莫大美, 吴荣书. 复合菌种发酵法制备玫瑰酵素工艺研究[J]. 食品工业,2016,37(10):64−69.
[15]	Navajas-Porras B, S. Pérez-Burillo, J. Morales-Pérez, et al. Relationship of quality parameters, antioxidant capacity and total phenolic content of EVOO with ripening state and olive variety[J]. Food Chemistry,2020,325:126926. doi: 10.1016/j.foodchem.2020.126926
[16]	Zhao P, Qi C, Wang G, et al. Enrichment and purification of total flavonoids from Cortex Juglandis Mandshuricae extracts and their suppressive effect on carbon tetrachloride-induced hepatic injury in mice[J]. Journal of Chromatography B,2015,1007:8−17. doi: 10.1016/j.jchromb.2015.10.019
[17]	张唯, 严成, 张曦, 等. 超高压提取玫瑰花色苷及稳定性研究[J]. 中国调味品,2018,43(8):151−157. doi: 10.3969/j.issn.1000-9973.2018.08.036
[18]	陈晨, 胡文忠, 田沛源, 等. 超声辅助提取香蕉皮多酚工艺优化及其抗氧化性的分析[J]. 食品科学,2014,35(2):12−17. doi: 10.7506/spkx1002-6630-201402003
[19]	Asma Lekouaghet, Abdelatif Boutefnouchet, Chawki Bensuici, et al. In vitro evaluation of antioxidant and anti-inflammatory activities of the hydroalcoholic extract and its fractions from LeuzeaconiferaL. roots[J]. South African Journal of Botany,2020,132:103−107. doi: 10.1016/j.sajb.2020.03.042
[20]	谭飔, 夏国灯, 韩杨, 等. 辣木籽多酚提取工艺优化及其抗氧化活性研究[J]. 食品科技,2019,44(1):280−285.
[21]	Wang Mei-Xue, Zhaoxue-Zhong, Gai Chun-Yu, et al. Antioxidativecapacity of Irbesartan[J]. Chemical Research in Chinese Universities,2011,27(1):75−79 (in Chinese).
[22]	方敏, 王耀峰, 宫智勇. 15种水果和33种蔬菜的抗氧化活性研究[J]. 食品科学,2008,29(10):97−100. doi: 10.3321/j.issn:1002-6630.2008.10.015
[23]	李加兴, 余娇, 黄诚, 等. 猕猴桃籽油的体外抗氧化活性[J]. 食品科学, 2012, 33(23): 51-54.
[24]	战伟伟, 魏晓宇, 高本杰, 等. 蓝靛果椰子复合酵素发酵工艺优化[J]. 中国酿造,2017,36(1):191−195.
[25]	陈艳艳, 于波, 潘黛安, 等. 乳杆菌发酵葛根水提液工艺研究及其解酒功效探讨[J]. 中国酿造,2020,39(8):182−186. doi: 10.11882/j.issn.0254-5071.2020.08.034
[26]	王瑜, 李立郎, 杨娟, 等. 刺梨酵素发酵工艺优化及发酵前后风味与活性成分分析[J]. 食品科技,2019,44(10):74−81.
[27]	洪厚胜, 朱曼利, 李伟, 等. 葡萄果渣酵素的发酵工艺优化及其理化特性[J]. 食品科学,2019,40(8):63−72. doi: 10.7506/spkx1002-6630-20180510-166
[28]	Kalt Wilhelmina, Hanneken Anne, Milbury Paul, et al. Recent research on polyphenolics in vision and eye health[J]. Journal of Agricultural and Food Chemistry,2010,58(7).
[29]	彭长连, 陈少薇, 林植芳, 等. 用清除有机自由基DPPH法评价植物抗氧化能力[J]. 生物化学与生物物理进展,2000,27(6):658−661. doi: 10.3321/j.issn:1000-3282.2000.06.022
[30]	周小琦, 方敏, 宫智勇. 玫瑰花中总黄酮提取工艺及其抗氧化与抑菌作用的研究[J]. 食品科学,2010,31(20):102−105.
[31]	沈清, 楼乐燕, 尹培, 等. 5 种梅干菜的酚类化合物及抗氧化能力比较分析[J]. 食品科学,2018,39(12):212−218. doi: 10.7506/spkx1002-6630-201812033
[32]	李杰, 赵声兰, 陈朝银. 核桃青皮果蔬酵素的成分组成及体外抗氧化活性研究[J]. 食品工业科技,2016,37(10):117−122.

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