Fluorescence Aptasensor for 17<i>β</i>-Estradiol Determination Based on Black Phosphorus Nanosheets

LIU Xiao; ZHU Chenglong; PANG Yuehong; FENG Yongwei; SHI Xueli; ZHANG Yi

doi:10.13386/j.issn1002-0306.2020090046

Volume 42 Issue 11

May 2021

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Science and Technology of Food Industry > 2021 > 42(11): 248-254. > DOI: 10.13386/j.issn1002-0306.2020090046

LIU Xiao, ZHU Chenglong, PANG Yuehong, et al. Fluorescence Aptasensor for 17 β -Estradiol Determination Based on Black Phosphorus Nanosheets [J]. Science and Technology of Food Industry, 2021, 42(11): 248−254. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020090046.

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Fluorescence Aptasensor for 17β-Estradiol Determination Based on Black Phosphorus Nanosheets

1.
Institute of Analytical Food Safety, College of Food Science and Technology, State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
2.
Food Safety Inspection and Testing Center of Wuxi, Wuxi 214122, China
3.
Maternal and Child Health Hospital of Shijiazhuang, Shijiazhuang 050051, China

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Received Date: September 09, 2020
Available Online: April 05, 2021

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Abstract

Abstract

A fluorescence aptasensor for quantitative detection of 17β-estradiol was constructed. Black phosphorus nanosheets (BPNs) were prepared by ultrasound-assisted aqueous phase exfoliation using black phosphorus crystal (BP) as fluorescence receptors. A 17β-estradiol fluorescence aptasensor was constructed between FAM labeled aptamers and BPNs based on the fluorescence resonance energy transfer (FRET). After optimization of BPNs concentration, FAM-Apt concentration and reaction time, samples of tap water and milk were determined. The results showed that the prepared BPNs had independent lamellar structure and uniform particle size.With the concentrations of BPNs and FAM-Apt were 10 μg/mL and 7.5 nmol/L respectively, the linear range of the sensor was 1.5~60 ng/mL and the detection limit was 1.0 ng/mL. The recoveries of tap water and milk were 91.2%~104.8% and 87.5%~104.3%. The relative standard deviation (RSD) were 4.99%~10.53% and 4.48%~11.24%, respectively. This method with simple, high sensitive and strong specificity could be completed within 30 min and could realize quantitative detection of E2 in actual samples.
- aptasensor,
- black phosphorus nanosheets,
- 17β-estradiol,
- fluorescence resonance energy transfer

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